suppression of a tomato prolyl 4 …...khansa mekkaoui thank you get in touch...
TRANSCRIPT
Mekkaoui, K.1, Perrakis A.1, Kaloudas D.1, Bita E.1, Krokida A.1, Giannoutsou E.2, Arhondakis S.1, Blazakis K.1, Imam, El H.1, Denic, D.1, Azariadis A.1 Kalaitzis, P.1
11Dept Horticultural Genetics & Biotechnology, Mediterranean Agronomic Institute of Chania (CIHEAM),Crete, Greece.
2Lab of Botany, Dept of Biology, Kapodistrian University of Athens, Athens, Greece.
SUPPRESSION OF A TOMATO PROLYL 4-HYDROXYLASE ALTERS FRUIT GROWTH PROGRAMS
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PROLYL 4-HYDROXYLASES
Proline hydroxylationGorres & Raines (2010) Crit. Rev. Biochem. Mol.
Proline Hydroxyproline
2-oxoglutarate Succinate
Proline
Secreted Peptide Hormones
Post-translational modifications of HRGPs
Eric Nguema-Ona et al., (2014) Frontiers in Plant Science
Marsubayashi (2011) Plant Cell Phys
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Suppression of P4Hs expression
Alteration of frequency of Proline hydroxylation
Alteration of substrates proteins
Alteration of developmentalprograms
Tomato fruit development
Cheniclet et al. (2005) Plant physiol
Azzi et al., (2015) JXB
Inhibition of Proline Hydroxylation induced degradation of a Proline Rich
Protein in Soybean cell culture
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Schmidt et al., 1991 Plant Phys.
Repetitive proline rich protein
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Control P4H1
P4H7 P4H9
VIGs suppression of 3 P4Hs
Fragostefanakis et al., Plant Mol Biol 2014
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Gene expression of tomato P4Hs during fruit growth
Suppression of expression by RNAi of tomato P4H3 during
fruit growth
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pHELLSGATE_12http://www.snapgene.com
Down-regulation of P4H3 decreases fruit size
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Expression of AGPs and EXTENSINS during fruit growth in RNAi lines
Text – Calibri, 16 pt
2 to 4 slides
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Expression of AGPs and EXTENSINS during fruit growth in RNAi lines
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Western blot analysis of AGPs and extensins during tomato fruit
growth of P4H3 RNAi lines
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Immunolocalization: 30 dpa fruit
Immunolocalization: 30 dpa fruit
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Conclusions
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P4H3 silencing
Alteration of fruit size
Alteration of AGPs and EXTsstructure/content
No alteration in AGPs and EXTs transcript levels
Thank YouKhansa Mekkaoui
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Department of Horticultural Genetics & Biotechnology - September 2018