Supplementary Figure 1

TA

K1

E14.5 E18.5

0.00

0.25

0.50

0.75

1.00

1.25

1.50

1.75

Tak

1 m

RN

A e

xpre

ssio

n

Sternal Chondrocytes

3 6 6 8 8 - - + - +

DayBMP

A

B

C

E14.5

Kn

ee

Prx1Cre; R26R

WT Tak1Col2

CT

X-R

ay

P0

E18.5

Alc

ian

Blu

e/

Ali

zari

n R

ed

WT Tak1Col2

A

B

Tak1Prx WT

4 weeks

C

Supplementary Figure 2

A1 A2

A3 A4

B1 B2

WT Tak1Col2 WT Tak1Col2

E14.5 E18.5

A

WT Tak1Prx WT Tak1Prx

E14.5 E18.5

Supplementary Figure 3

C1 C2 C3 C4

A1 A2 A3 A4

E18.5

WT Tak1Col2B

C

B1 B2

D5

D3

D

1 DMSO TI-2

Alkaline Phosphatase

pSmad 1/5/8

tSmad 1/5/8

-actin

BMP - + - +

DMSOTI-2

+ + - - - - + +

Supplementary Figure 4

A B

DMSO TI-2 TI-2 - - 1 3 0.1 1 3 5

DMSO + + - - - - - -

BMP2 - + - - + + + +

12x SBE - LucC

p-Smad 1/5/8t-Smad 1/5/8

BMP2 - + + + + + TI-2 - - 0.1 1 3 5

p-p38t-p38

tubulin

C1 C2Noggin

* *

*

C3

Fo

ld In

du

ctio

n

0

150

50

100

200 UntreatedBMP

0

110

75

402

Fo

ld In

du

ctio

n