Supplementary Figure 1

0

-1

-2

-3

-4

0

-1

-2

-3

-4

SU11274 PHA665752 SGX523827 + EGF/bFGF 827 + EGF/bFGF 827 + EGF/bFGF

827 + HGF 827 + HGF 827 + HGFSU11274 PHA665752 SGX523

A.

B.

100806040200

50403020100

100806040200

0 5 10 15 20

0 50 100 150 200

0 50 100 150 200

number of plated cells

131 + EGF/bFGF 131 + EGF/bFGF 131 + EGF/bFGF

131 + HGF 131 + HGF 131 + HGF

0

-1

-2

-3

-4

0

-1

-2

-3

-4

number of plated cells

SU11274 PHA665752 SGX523

SU11274 PHA665752 SGX523

0 50 100 150 200

0 50 100 150 200

0 50 100 150 200

5004003002001000

0 50 100 150 200

0 20 40 60 80 100

Pro

po

rtio

n o

f w

ells

w

/o s

ph

ere

(lo

g1

0)

Pro

po

rtio

n o

f w

ells

w

/o s

ph

ere

(lo

g1

0)

Pro

po

rtio

n o

f w

ells

w

/o s

ph

ere

(lo

g1

0)

Pro

po

rtio

n o

f w

ells

w

/o s

ph

ere

(lo

g1

0)

2.08% 31.8% 25.4%

2.83%9.00% 7.53%

Iso

typ

e-A

PC

Iso

typ

e-A

PC

CD

133-

AP

CC

D13

3-A

PC

464T+EGF/bFGF

464T+EGF/bFGF

-SU11274

464T+EGF/bFGF

+SU11274

464T+HGF

464T+HGF

-SU11274

464T+HGF

+SU11274

Supplementary Figure 2

GFP ABC Merge Merge + DAPI

Supplementary Figure 3

Fie

ld 1

Fie

ld 2

Fie

ld 3

GFP ABC Merge Merge + DAPI

GFP ABC Merge Merge + DAPI

mCMV backbone reporter

mCMV-TCF/LEF reporter

464T

Rel

ativ

e L

uci

fera

se U

nit

Wnt3a(ng/ml)

0 25 50

Supplementary Figure 4

Supplementary table 1

Symbol Name

Fold increase in 464T

METhigh/+ cells

Fold increase in 578T

METhigh/+ cells References

CD44 CD44 antigen 3.59 12.22 Am J Pathol, 1999, 154(2):515

MET MET protooncogene 1.99 19.65 Cancer Res, 2002, 62(18):5126

PLAUR Plasminogen activator receptor, urokinase-type 2.02 2.74 Proc Natl Acad Sci USA, 1999, 96(4):1603

CCND1 Cyclin D1 2.41 1.82 Nature, 1999, 398(6726):422

NRCAM Neuronal cell adhesion molecule 2.28 1.19 Genes Dev, 2002, 16(16)2058

FOSL1 Fos-like antigen 1 1.55 1.42 Proc Natl Acad Sci USA, 1999, 96(4):1603

JUNv-jun avian sarcoma virus 17 oncogene homolog

1.10 1.90 Proc Natl Acad Sci USA, 1999, 96(4):1603

L1CAM L1 cell adhesion molecule 1.12 1.76 J Cell Biol, 2005, 168(4):633

JAG1 Jagged 1 1.15 1.50 Proc Natl Acad Sci USA, 2009, 106(4)633

CLDN1 Claudin 1 1.28 1.21 Oncol Res, 2001, 12(11-12):469

TCF7 Transcription factor, T cell-specific 1 1.38 1.11 Science, 1999, 285(5435):1923

MYC Oncogene myc 1.08 1.37 Science, 1998, 281(5382):1509

BIRC5Baculoviral IAP repeat-containing protein 5 (Survivin)

1.2 1.00 Cancer Res, 2001, 61(24)8664

LEF1 Lymphoid enhancer-binding factor 1 0.91 2.85 J Biol Chem, 2002, 277(36)33398

BMP4 Bone morphogenetic protein 4 1.41 0.86 Cancer Res, 2002, 62(10)2744

FGF18 Fibroblast growth factor 18 1.30 0.92 Cancer Res, 2003, 63(19)6116

GAST Gastrin 1.45 0.77 J Clin Invest, 2000, 106(4)533

Expression levels of WNT target genes in METhigh/+ cells