supplementary data d a c d ab b figure s1. quantification of the western blot data in figure 4c. the...
TRANSCRIPT
Supplementary data
d
a
c
d
abb
Figure S1. Quantification of the Western blot data in figure 4C. The area density of each band was measured with a UVP gel document system. The data were normalized with β-tubulin and presented as ratio relative to the area density of β-tubulin. Bars represent means ± SEM. Bars with the same letters are not significantly different from each other (p>0.05).
00.
10.
5 1 2 50.0
0.2
0.4
0.6
G-1 (M)
BC
L-2
(OD
re
lati
ve
to
-a
cti
n)
00.
10.
5 1 2 50.00
0.02
0.04
0.06
0.08
0.10
G-1 (M)
cle
av
ed
-F
od
rin
(OD
re
lati
ve
to
-a
cti
n)
b
cdd
c
aa
00.
10.
5 1 2 50.0
0.2
0.4
0.6
G-1 (M)
cle
av
ed
PA
RP
(OD
re
lati
ve
to
-a
cti
n)
b
cc
c
a a
b
dc
d
a a
00.
10.
5 1 2 50.0
0.5
1.0
1.5
G-1 (M)
P2
1 C
IP1
(OD
re
lati
ve
to
-a
cti
n)
00.
10.
5 1 2 50.0
0.2
0.4
0.6
0.8
1.0
G-1 (M)
su
rviv
in(O
D r
ela
tiv
e t
o -
ac
tin
)
ba
c
dd
a
Supplementary data
Figure S2. Quantification of the Western blot data in figure 5C. The area density of each band was measured with a UVP gel document system. The data were normalized with β-tubulin and presented as ratio relative to the area density of β-tubulin. Bars represent means ± SEM. Bars with the same letters are not significantly different from each other (p>0.05).
00.
10.
5 1 2 50.0
0.5
1.0
1.5
2.0
G-1 (M)
Su
rviv
in(O
D r
ela
tiv
e t
o -
tub
ulin
)
caba a
bcab
00.
10.
5 1 2 50.00
0.05
0.10
0.15
0.20
G-1 (M)
cle
av
ed
PA
RP
(OD
re
lati
ve
to
-t
ub
ulin
)
a a a
b
c
b
00.
10.
5 1 2 50.00
0.05
0.10
0.15
0.20
G-1 (M)
cle
av
ed
-f
ord
rin
(OD
re
lati
ve
to
-tu
bu
lin)
bb
b
a aa
00.
10.
5 1 2 50.0
0.2
0.4
0.6
0.8
G-1 (M)
BC
L-2
(OD
re
lati
ve
to
-tu
bu
lin)
b
dc
a a a
00.
10.
5 1 2 50.0
0.2
0.4
0.6
G-1 (M)
P2
1 C
IP1
(OD
re
lati
ve
to
-tu
bu
lin)
a a
d
b
e
c
Supplementary data
Figure S3. Quantification of the Western blot data in figure 6A. The area density of each band was measured with a UVP gel document system. The data were normalized with β-tubulin and presented as ratio relative to the area density of β-tubulin. Bars represent means ± SEM. Bars with the same letters are not significantly different from each other (p>0.05).
00.
10.
5 1 2 50.0
0.5
1.0
1.5
G-1 (M)
p-H
isto
ne
H3
(Se
r10
)(O
D r
ela
tiv
e t
o -
tub
ulin
)
b
dc
cd
a a
00.
10.
5 1 2 50.0
0.2
0.4
0.6
0.8
1.0
G-1 (M)
p-N
UM
A(O
D r
ela
tiv
e t
o -
tub
ulin
)
b
cc
c
a a
00.
10.
5 1 2 50.0
0.5
1.0
1.5
G-1 (M)
p-A
uro
ra A
/B/C
(OD
re
lati
ve
to
-tu
bu
lin)
b
dc
d
a a
00.
10.
5 1 2 50.0
0.5
1.0
1.5
G-1 (M)
p-W
ee
1(O
D r
ela
tiv
e t
o -
tub
ulin
)c
b
d d
bca
00.
10.
5 1 2 50.0
0.5
1.0
1.5
G-1 (M)
p-C
DC
25
(T
48
)(O
D r
ela
tiv
e t
o -
tub
ulin
)
b
d
c
d
a a
00.
10.
5 1 2 50.0
0.1
0.2
0.3
0.4
0.5
G-1 (M)
p-C
DC
2 (
Y1
5)
(OD
re
lati
ve
to
-tu
bu
lin)
b
c
b
c
a a
Supplementary data
Figure S4. Quantification of the Western blot data in figure 6B. The area density of each band was measured with a UVP gel document system. The data were normalized with β-tubulin and presented as ratio relative to the area density of β-tubulin. Bars represent means ± SEM. Bars with the same letters are not significantly different from each other (p>0.05).
00.
10.
5 1 2 50.0
0.2
0.4
0.6
0.8
G-1 (M)
p-A
uro
ra A
/B/C
(OD
re
lati
ve
to
-tu
bu
lin)
b
dc
d
a a
00.
10.
5 1 2 50.0
0.2
0.4
0.6
0.8
G-1 (M)
p-w
ee
1(O
D r
ela
tiv
e t
o -
tub
ulin
)b bb b
aa
00.
10.
5 1 2 50.0
0.2
0.4
0.6
G-1 (M)
p-N
UM
A(O
D r
ela
tiv
e t
o -
tub
ulin
)
c
dd
a ab b
00.
10.
5 1 2 50.0
0.5
1.0
1.5
G-1 (M)
p-H
isto
ne
H3
(Se
r10
)(O
D r
ela
tiv
e t
o -
tub
ulin
)
b
d
c
ef
a
00.
10.
5 1 2 50.0
0.2
0.4
0.6
0.8
G-1 (M)
p-c
dc
25
(T
48
)(O
D r
ela
tiv
e t
o -
tub
ulin
)
b
dc
a a a
00.
10.
5 1 2 50.0
0.1
0.2
0.3
0.4
0.5
G-1 (M)
p-c
dc
2 (
Y1
5)
(OD
re
lati
ve
to
-tu
bu
lin)
b
d
c
d d
a