Supplemental Material:

Supplement table 1: Physico-chemical parameters of untreated and treated wastewaters were analysed according to APHA standard methods

protocol.

Parameters Petrochemical

inlet to ETP

Petrochemical

outlet from ETP

Petrol refinery

Inlet to ETP

Petrol refinery

Outlet from

ETP

Coke

oven Inlet

to ETP

Coke oven

outlet from

ETP

Tolerance limit

for Effluent std,

BIS

Tolerance limit

for Drinking

water

pH 9.1 6.6 9.1 6.8 8.50 7.10 5.5-9.0 6.5-8.5

Temperature [°C] 33.0 31.0 36.0 31.0 32.00 28.70 40 -

Dissolved oxygen [mg/l] 3.3 1.2 3.3 1.8 3.17 1.42 - -

Theoretical COD [mg/l] 3240 180 4540 261.0 810.30 225.39 250 -

HeadspaceBOD [mg/l] 55 10 200 31.0 92.30 21.90 30 -

Oil and grease [mg/l] 2.2 2 34.3 28.4 2.23 1.02 10 .01

Dissolved solids [mg/l] 3243.45 2062 3400 2100 1287.82 945.00 2100 500

Ammonical nitrogen [mg/l] 533.90 48.2 658.0 98.0 562.30 89.93 50 100

ParaNitrate nitrogen [mg/l] 56.16 10.7 66.16 46.0 55.39 23.21 - 45

Total hardness [mg/l] 518.19 272.87 650.19 305.0 495.39 140.21 - 300

S Phenol(mg/l) 128.47 0.95 142.47 1.05 123.60 0.60 1.0 0.001

Cyanide [mg/l] 1.3 0.18 1.9 0.2 20.70 0.20 0.2 0.05

A

B

Supplement Figure 1: A) Schematic of the studied petrochemical industry effluent treatment plant. B) Schematic of the studied steel industry effluent treatment plant.

1 2 3 4 5

Supplement Figure 2: DNA fragmentation of genomic DNA isolated from cultured cells

treated with the Petrochemical sludge extract. A representative result of three sets of

experiments is shown. Lane 1: Positive control benzo[a]pyrene; Lane 2: sludge extract 200 μl;

Lane 3: sludge extract 100 μl; Lane 4: sludge extract 50 μl; Lane 5: negative control (DMSO);

Lane 6: Molecular weight marker.

Supplement Figure 3: Immunoblot analysis showing P53 protein accumulation in cell

cultures exposed to petrochemical sludge extract. Lane 1: negative control; Lane 2: sludge

extract 50 μl; Lane 3: sludge extract 100 μl; Lane 4: sludge extract 200 μl; Lane 5: positive

control benzo[a]pyrene

Supplement Figure 4: DNA fragmentation of genomic DNA isolated from cultured cells

treated with the Petrochemical effluent water extract. A representative result of three sets of

experiments is shown. Lane 1: Molecular weight marker; Lane 2: Control (DMSO); Lane 3:

effluent extract 50 μl; Lane 4: effluent extract 100 μl; Lane 5: effluent extract 200 μl and Lane

6: Positive control benzo[a]pyrene

Supplement Figure 5: Immunoblot analysis showing P53 protein accumulation in cell

cultures exposed to petrochemical effluent water extract Lane 1: negative control; Lane 2:

effluent extract 50 μl; Lane 3: effluent extract 100 μl; Lane 4: effluent extract 200 μl; Lane 5:

positive control benzo[a]pyrene

1 2 3 4 5

Supplement Figure 6: DNA fragmentation of genomic DNA isolated from cultured cells

treated with the petroleum refinery sludge extract. A representative result of three sets of

experiments is shown. Lane 1: Molecular weight marker; Lane2: Control (DMSO); Lane 3:

sludge extract 50 μl; Lane 4: sludge extract 100 μl, Lane 5: sludge extract 200 μl; Lane 6:

Positive control benzo[a]pyrene

Supplement Figure 7: Immunoblot analysis showing P53 protein accumulation in cell

cultures exposed to petroleum refinery sludge extract. Lane 1: negative control; Lane 2:

sludge extract 50 μl; Lane 3: sludge extract 100 μl; Lane 4: sludge extract 200 μl; Lane 5:

positive control benzo[a]pyrene

Supplement Figure 8: DNA fragmentation of genomic DNA isolated from cultured cells

treated with the petroleum refinery effluent water extract. A representative result of three sets

of experiments is shown. Lane 1: Molecular weight marker; Lane 2: Control (DMSO); Lane

3: effluent extract 50 μl; Lane 4: effluent extract 100 μl; Lane 4: effluent extract 100 μl, Lane

5: effluent extract 200 μl and Lane 6: Positive control benzo[a]pyrene

Supplement Figure 9: Immunoblot analysis of P53 expression in cell cultures exposed to

petroleum refinery effluent water extracts. Lane 1: negative control; Lane 2: effluent extract

50 μl; Lane 3: effluent extract 100 μl; Lane 4: effluent extract 200 μl; Lane 5: positive control

benzo[a]pyrene

Supplement Figure 10: DNA fragmentation of genomic DNA isolated from cultured cells

treated with coke oven wastewater extract before treatment. A representative result of three

sets of experiments is shown. Lane 1: Positive control benzo[a]pyrene; Lane 2: water extract

50 μl; Lane 3: water extract 25 μl; Lane 4: water extract 10 μl, Lane 5: Control (DMSO) and

Lane 6: Molecular weight marker

Supplement Figure 11: Immunoblot analysis of P53 expression in cell cultures exposed to

coke oven wastewater extract before treatment. Lane 1: positive control benzo[a]pyrene; Lane

2: water extract 50μl; Lane 3: water extract 25 μl; Lane 4: water extract 10μl; Lane 5:

negative control.

Supplement Figure 12: DNA fragmentation of genomic DNA isolated from cultured cells

treated with the coke oven effluent water extract after treatment. A representative result of

three sets of experiments is shown. Lane 1: Molecular weight marker; Lane 2: effluent extract

10 μl; Lane 3: effluent extract 25 μl; Lane 4: effluent extract 50 μl, Lane 5: Control (DMSO)

and Lane 6: Positive control benzo[a]pyrene

Supplement Figure 13: Immunoblot analysis of P53 expression in cell cultures exposed to coke

oven effluent water extract after treatment. Lane 1: negative control; Lane 2: effluent extract 10

μl; Lane 3: effluent extract 25 μl; Lane 4: effluent extract 50 μl; Lane 5: positive control

Benzo[a]pyrene.

Supplement Figure 14: Cytotoxicity assay A. Sludges Extract (Petrochemical industy) B. Watewater Extract (Petrochemical industry) C. Sludges Extract (Petro refinery) D. ETP Sludges Extract (Petro refinery) E. Watewater Extract (Petro refinery) F. Watewater Extract (Steel industry before treatment) G. Watewater Extract (Steel industry after treatment)