Supplemental data

ATP citrate lyase inhibition can suppress tumor cell growth Georgia Hatzivassiliou, Fangping Zhao, Daniel E. Bauer, Charalambos Andreadis, Anthony N. Shaw, Dashyant Dhanak, Sunil R. Hingorani, David A. Tuveson, and Craig B. Thompson

Figure S1. The sensitivity profile of wild-type and cytochrome c-null cells to ACL inhibition by SB-204990 in vitro correlates with their levels of glucose-dependent lipid synthesis A: Wild-type and cytochrome c-null cell lines were pre-incubated in medium plus 1% FCS for 24 hours and the level of their glucose-dependent lipid synthesis was determined as described in the Experimental Procedures. B: The in vitro sensitivity of wild-type and cytochrome c-null cells to SB-204990 was determined by the SRB assay. OD 565 values of SB-204990 treated cells were normalized to the values obtained for vehicle-treated samples after a 1-day treatment. Plotted is the mean ± SD of triplicate samples from a representative experiment.

Figure S2. Pharmacological inhibition of ACL by SB-204990 in tumor xenografts in vivo is associated with increased tumor differentiation A–C: Glandular differentiation is detected by H&E staining (A), mucin production is detected by mucicarmine staining (B) and caspase activation is detected by immunohistochemistry using an anti-cleaved caspase 3 antibody (C) in A549 xenografts from individual mice treated with vehicle or SB-204990. Original magnification is 200×.