supernova for cali technique
TRANSCRIPT
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SUPERNOVA A MONOMERIC PHOTOSENSITIZING FLUORESCENT PROTEIN FOR
CHROMOPHORE-ASSISTED LIGHT INACTIVATION
KIWAMU TAKEMOTO ET AL. 2013SCIENTIFIC REPORTS
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PHOTOSENSITIZERS
Bulina et al., 2006
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CALI CHROMOPHORE-ASSISTED LIGHT INACTIVATION
Photosensitizer ProteinTarget Protein
intense light irradiation Target inactivation
Cellular Death
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PHOTOSENSITIZERS
Li et al., 2012
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KILLER RED VS SUPER NOVA
Takemoto et al., 2013, Bulina et al., 2006
579 nm610 nm
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SuperNova KillerRed EGFP
Cx43
Keratin
Fibrillarin
KILLER RED VS SUPER NOVA
Scale bar 10µmTakemoto et al., 2013,
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EXPERIMENTAL RESULTS
0 15 30
45 60 75 90
Takemoto et al., 2013, Bulina et al., 2006Magnification 100x
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EXPERIMENTAL RESULTSActin-GFP Cofilin-SuperNova
BeforeCALI
AfterCALI
Takemoto et al., 2013Scale bar 10µm
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CALI APPLICATIONS
Metaphase Early Cytokinesis Late Cytokinesis Death Survival
Ou et al., 2012
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CALI IN C.ELEGANS
Scale bar 5µm Scale bar 2,5µm
WT CALI QR.a QR.a (pig-1) QR.p
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REFERENCES
Wei Li, Nico Stuurman, Guangshuo Ou. (2012). Chromophore-assisted laser inactivation in neural development. Neuroscience Bulletin. 28 (4), 333–341.
Maria E Bulina, Konstantin A Lukyanov, Olga V Britanova, Daria Onichtchouk, Sergey Lukyanov & Dmitriy M Chudakov. (2006). Chromophore-assisted light inactivation (CALI) using the phototoxic fluorescent protein KillerRed. Nature Protocols. 1 (2), 947-953.
Maria E Bulina, Dmitriy M Chudakov, Olga V Britanova, Yurii G Yanushevich, Dmitry B Staroverov, Tatyana V Chepurnykh, Ekaterina M Merzlyak, Maria A Shkrob Sergey Lukyanov & Konstantin. (2006). A genetically encoded photosensitizer. Nature Biotechnology. 24 (1), 95-99.
Guangshuo Ou, Nico Stuurman, Michael D’Ambrosio, Ronald D. Vale. (2010). Polarized Myosin Produces Unequal-Size Daughters During Asymmetric Cell Division. Science. 330, 677-679.
Takemoto, K., Matsuda, T., Sakai, N., Fu, D., Noda, M., Uchiyama, S., ... & Nagai, T. (2013). SuperNova, a monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation. Scientific reports, 3.
Look for diagnosis.com (2014). Chromophore-Assisted Light Inactivation (CALI) using the phototoxic fluorescent protein KillerRed. Available: http://jcs.biologists.org/content/127/8/1621/F1.large.jpg.