Molecular Constellations 2015

Superresolution Fluorescence Microscopy

C. Cremer

Institute of Molecular Biology (IMB), Mainz/DE

Institute for Pharmacy & Molecular Biotechnology (IPMB)/Kirchhoff-Institute for

Physics (KIP), University Heidelberg

For more information see www.optics.imb-mainz.de

L

etizia

Mancin

o

Heidelberg Mainz

Superresolution Microscopy* of DNA

Distribution in a human Cell Nucleus.

Intensity Image

after Labelling

with a DNA

specific

Fluorochrome:

~ 5,000 DNA

sites /µm2

Maximum ~ 27,000

DNA sites/µm2

Szczurek et al. (2014) *Localization Microscopy (SPDM)

SPDM 4

Superresolution

Microscopy* of

Human

Influenza A

Virus (IAV)

associated with

a human Cell

Membrane

*Localization

Microscopy

(SPDM)

Cremer et al. 2014

Counting of

individual IAV*

along membrane:

N ~ 100

*cluster Ø ~ 120

nm

3 point-like objects in x,y plane with

next neigbour distances 50 nm

a) Labelling with same spectral

signature

b) Labeling with different unique

spectral signatures B,G,R

Computation (scalar Theory):

NA = 1.4, λexc = 488 nm

Optical Isolation by the

“Supernova“ Mode: Induction of

stochastically distributed short

flashes of light emission by

individual molecules Cremer et al. 1996, 1999, 2010, 2011

Principle of Localization Microscopy based Superresolution

(Spectral Precision Distance Microscopy/SPDM)

For details see www.optics.imb-mainz.de

Nobel Prize 2014 awarded to former Cremer-

Lab Associate Prof. Stefan Hell

A former associate of the Cremer Lab, Prof. Dr. Stefan

Hell, was awarded the Nobel Prize 2014 in Chemistry

for his work on advanced far field light microscopy.

Three scientists share the award: Two Scientists from

USA, Prof. Eric Betzig and Prof. William Moerner, for

developing single molecule microscopy using

photoactivable proteins, and Prof. Stefan Hell from

Germany, who has been awarded for the development

of Stimulated Emission Depletion Microscopy, a

"focused nanoscopy" method (Scientifc background

given at www.nobelprize.org).

Publications Cremer Lab 2014 part I

A.T. Szczurek, K. Prakash, H. K. Lee, D. J. Żurek-Biesiada, G. Best, M.

Hagmann, J. W. Dobrucki, C. Cremer, U. Birk (2014) Single molecule

localization microscopy of the distribution of chromatin using Hoechst and DAPI

fluorescent probes. Nucleus 5 (4): 1-10.

T. Cremer, C. Cremer, P. Lichter (2014) Recollections of a scientific journey

published in Human Genetics: from chromosome territories to interphase

cytogenetics and comparative genome hybridization. Hum Genet (2014)

133:403–416.

Q. Wang, R. Dierkes, R. Kaufmann, C. Cremer (2014) Quantitative analysis of

individual Hepatocyte Growth Factor Receptor Clusters in Influenza A virus

infected human epithelial cells using localization microscopy, Biochimica et

Biophysica Acta 1838: 1191–1198.

C. Cremer, R. Kaufmann, M. Gunkel, F. Polanski, P. Müller, R. Dierkes, S.

Degenhard, C. Wege, M. Hausmann, U. Birk (2014) Application Perspectives of

localization microscopy in Virology. Histochem. Cell. Biol., DOI

10.1007/s00418-014-1203-4.

Publications Cremer Lab 2014 part II

P. Müller, N. A. Lemmermann ,• R. Kaufmann , M. Gunkel, D. Paech , G.

Hildenbrand , R. Holtappels, C. Cremer, M. Hausmann (2014) Spatial

distribution and structural arrangement of a murine cytomegalovirus

glycoprotein detected by SPDM localization microscopy. Histochem Cell Biol

DOI 10.1007/s00418-014-1185-2.

C. Cremer (2014) Far‐field Light Microscopy. Encyclopedia of Life Sciences

(eLS), DOI:10.1002/9780470015902.a0005922.pub2.

C. Cremer (2014) Vorstoss in atomare Dimensionen. Laborjournal 7-8/2014: 65

– 67.

A. L. Grab, M. Hagmann, R. Dahint, C. Cremer (2015) Localization microscopy

(SPDM) facilitates high precision control of lithographically produced

nanostructures. Micron 68: 1-7.

C. Cremer Lab (2014)

Udo Birk (Deputy GL) Margund Bach

Gerrit Best Zhe Chen

Johann von Hase Florian Schock

Anna Luise Grab Hyun-Keun Lee

Martin Hagmann Ramon Perez

Dongyu Ma Kirti Prakash

Jan Neumann Wladimir Schaufler

Dimka Pieper-Krasteva Mei Yu Chen

Aleksander Szczurek Dominik Waibel

Sabrina Rossberger

Alexander Saroori

Collaboration Partners 2014

Heike Allgayer, Univ. Heidelberg/DKFZ

Thomas Cremer, Univ. Muenchen (LMU)

Stefan Dithmar, Univ. Heidelberg/Wiesbaden

Michael Hausmann, Univ. Heidelberg

Reiner Heintzmann, Univ. Jena Peter Huber, DKFZ Heidelberg

Rene Ketting, IMB, Mainz

George Reid, IMB Mainz John Stamatoyannopoulos, Univ. Washington

Letiz

ia M

ancin

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