stem cell identification
DESCRIPTION
In The Name of God. Stem Cell Identification. Nozad H. Stem cell workshop Stem cell Research center Tabriz medical university. Stem Cell Characteristics. ‘Blank cells’ ( unspecialized ) 2.Capable of dividing and renewing themselves for long periods of time - PowerPoint PPT PresentationTRANSCRIPT
Stem Cell Identification
Nozad H.Stem cell workshop
Stem cell Research center Tabriz medical university
In The Name of God
Stem Cell Characteristics
1. ‘Blank cells’ (unspecialized)
2.Capable of dividing and renewing themselves for long periods of time
(proliferation and self renewal)
3. Have the potential to give rise to specialized cell types (differentiation)
4. Plasticity and Cell based therapies
5. Very low percentage available(0.01-0.03%)
How we can identify Stem cells
1. In vitro single cell colony assay
2. Single cell PCR , looking for upregulation and downregulation of lineage related genes
3. In Vivo assay( Stem cell Transplantation)
Sorting single cell stem cell to evaluate the lineage potential
Co-cultures
Analysis
Single stem cell
OP9 cell line
For 3 to 5 weeksOP9DL1 cell line
Hematopoiesis - Blood Cell Formation
Myeloid Lymphoid
ErythrocytePlatelet
MacrophageGranulocyte
NK cell B cell T cellSelf-renew
al
Stem Cells
Committed progenitors
Mature cells
Bone
mar
row
Thymus,Blood,Spleen
CD34-Flt3-CD150+Lin-ScahighKithigh
CD34+Flt3- CD150-Lin-ScahighKithigh
LT-HSC
ST-HSC
MPPs CD34+Flt3+Lin-ScahighKithigh
Identifying mesenchymal stem cell 1. A heterogeneous population, mixed population of fibroblast-like plastic-adherent cells
2. differentiate into a range of mature connective tissue cell types .
3. MSCs have been isolated from skeletal muscle, adipose tissue, deciduous teeth,
4. No surface antigen individually, or in combination, has been unified as a “supreme” marker
5. A supportive role to HSCs during hematopoiesis.
6. expressed markers on MSC depending of the source of MSCs
7. MSC do not express haematopoietic and endothelial cell markers
Neural Stem cell
1. Express CD1332. Express low level of CD24 and PNA(peanut agglotinin in SVZ mic3. CXCR4, FGF R4 , BMI-1, Brg1, FABP7, Nestin 3. Combination of this marker by Nestin gene expression is the best NSCs