Contact our research specialists to learn more North America: +1 781 376 4150 • Rest of world: +45 45 650 929 support@exiqon.com • www.exiqon.com

Amplifi cation of a synthetic microRNA was performed using the miRCURY™

LNA microRNA PCR system. Hsa-miR-100 (2 × 108 copies) input was used

to generate cDNA. A ten-fold serial dilution ranging from 2 × 109 copies to

2 copies cDNA was amplifi ed by real-time PCR. A. The amplifi cation curves

and B. the standard curve show excellent linear correlation between the

decreasing cycle numbers and the logarithm of the microRNA copy number,

proving the assay enables sensitive microRNA detection down to less than

10 copies with a wide dynamic range of at least 8 logs.

Accurate quantifi cation over a wide dynamic range of microRNA copy numbers

Marie-Louise Lunn, Ph.D.

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R2 = 0.994Amplification efficiency 100%

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2 copies

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B

Start your microRNA discovery from a single cell MicroRNAs are small wonders. To study them, life science

researchers need extremely accurate and sensitive tools

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With the new miRCURY™ LNA microRNA PCR system,

your discovery starts from 10 pg total RNA – you only need

a single cell. You can even detect high and low expressed

microRNAs from the same sample in your experiment, as

the dynamic range is 8 logs wide.

Our PCR system combines microRNA-specifi c primers

and SYBR® Green detection and is part of Exiqon’s popular

miRCURY™ LNA product line, the most complete range of

tools for microRNA analysis available today.

The miRCURY™ LNA tools enable you to seek, fi nd and

verify microRNAs. Accelerate your discoveries.

Marie-Louise Lunn, Ph.D., Product Manager

9133

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To order or request additional information, please visit our Web site or:Call: 1-800-843-4388 (Continental US and Canada) 516-422-4100 (All other locations)FAX: 516-422-4097 E-mail: cshpress@cshl.eduWrite: Cold Spring Harbor Laboratory Press, 500 Sunnyside Blvd., Woodbury, NY 11797-2924

CONTENTS

PART 1: CHEMISTRY ANDGENETICS

1. The Mendelian View of the World2. Nucleic Acids Convey Genetic

Information3. The Importance of Weak Chemical

Interactions4. The Importance of High-Energy

Bonds5. Weak and Strong Bonds

Determine MacromolecularStructure

PART 2: MAINTENANCE OFTHE GENOME

6. The Structures of DNA and RNA

7. Genome Structure, Chromatin,and the Nucleosome

8. The Replication of DNA9. The Mutability and Repair of

DNA10. Homologous Recombination at the

Molecular Level11. Site-Specific Recombination and

Transposition of DNA

PART 3: EXPRESSION OF THEGENOME

12. Mechanisms of Transcription13. RNA Splicing14. Translation15. The Genetic Code

PART 4: REGULATION

16. Transcriptional Regulation inProkaryotes

17. Transcriptional Regulation inEukaryotes

18. Regulatory RNAs19. Gene Regulation in Development

and Evolution20. Genome Analysis and Systems

Biology

PART 5: METHODS

21. Techniques of Molecular Biology22. Model Organisms

INDEX

By James D. Watson, Cold Spring Harbor Laboratory, Tania A. Baker,Massachusetts Institute of Technology, Stephen P. Bell, Massachusetts Institute of

Technology, Alexander Gann, Cold Spring Harbor Laboratory, Michael Levine, University ofCalifornia, Berkeley, and Richard Losick, Harvard University

This sixth edition of James D. Watson’s classic textbook Molecular Biology of the Gene has beenthoroughly revised and updated. Accessible to anyone interested in molecular biology and genet-

ics, the book provides a historical basis for the field, concise descriptions of fundamental chemicalconcepts, a comprehensive survey of genome maintenance and expression, and a discussion of stan-dard techniques and model organisms commonly used in molecular biology studies. It includes allnew chapters on the regulatory RNAs and genomics and systems biology. The book has an accompa-nying Web site www.aw-bc.com, which contains interactive tutorials, animations, and critical-think-ing exercises designed to help students explore and visualize complex concepts.

2008, 841 pp., illus., indexHardcover $151 ISBN 978-080539592-1

This book is co-published with Benjamin Cummings. Requests for textbook examination copies, orders for course adoptions, and ALL individual orders outside of the U.S. should be sent to Benjamin Cummings at www.aw-bc.com.

The Power to Question

© 2006-2007 Santa Cruz Biotechnology, Inc., all rights reserved. "Santa Cruz Biotechnology" and the Santa Cruz Biotechnology, Inc. logo are registered trademarks of Santa Cruz Biotechnology, Inc.

santa cruz biotechnology, inc.

cell signaling research antibodies and siRNA gene silencers

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Zoom in on new targetsin cancer research

LightCycler® 480 Real-Time PCR System

Today’s cancer research requires increasingly accurate and versatile tools to

shed light on genetic and epigenetic variation patterns. Choose the

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■ miRNA Analysis in B-Cell Lymphoma

■ Analysis of Transcriptional Repression

View recently published studies using the LightCycler® 480 System for these

and other applications at www.lightcycler480.com

For general laboratory use. Not for use in diagnostic procedures.

This LightCycler® 480 Real-Time PCR System is licensed under U.S. Patent 6,814,934and corresponding claims in its non-U.S. counterparts and under one or more of U.S.Patents Nos. 5,038,852, 5,656,493, 5,333,675, or corresponding claims in their non-U.S.counterparts, for use in life science, by implication or by estoppel under any patentclaims or for any other implication. The product is covered in-part by US 5,871,908, co-exclusively licensed from Evotec OAI AG. Parts of the Software used for theLightCycler® 480 System are licensed from Idaho Technology Inc., Salt Lake City, UT,USA. LIGHTCYCLER and HRM are trademarks of Roche. Other brands or productnames are trademarks of their respective holders.

© 2008 Roche Diagnostics GmbH. All rights reserved.

Analysis of tumor suppressor gene methylationwith the LightCycler® 480 System. Mixtures ofunmethylated and fully methylated genomic DNAwere treated with bisulfite. A fragment of thePPP3CC tumor suppressor gene was then amplifiedand analyzed by high-resolution melting. Thisallowed semi-quantitative determination of theportion of methylated DNA contained in each case.

Roche Diagnostics GmbHRoche Applied Science68298 Mannheim, Germany