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Staining of ConnectiveTissue

Muscles and Bones

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STAIN BASICCOMPONENT

RESULTS

Gomoris

Silver

Impregnation

Silver nitrate

black

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Van

Geisons

Picric acid

and acid

fuchsinNuclei=brownish black to black

Collagen(fibrous connective

tissue)=pink;deep red

Muscle,cytoplasm,RBC and

fibrin=yellow

MassonsTrichome

NS=ironhematoxylin

CS=red dye Muscle,RBC and keratin=redNuclei=blue-black

Collagen and mucus=blue

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Mallorys

aniline Blue

Collagen fibrils,cytoplasm,fibroglia

fibrils,axon cylinders and

neuroglia=redEF=pale pink or yellow

RBC and myelin sheets=yellow

Azocarmine Azocarmine

dye

Amyloid c,.t and mucous

colloid=deep blue

Nuclei=red

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Weigerts Elastic

Tissue

Basic

fuchsin,resorcin,

ferric chloride

EF=dark blue or blue-black

Verhoeffs

EF=black;Nuclei=gray to black;Collagen=redCytoplasm and muscle=yellow

Orcein(Taenzer-

Unna Orcein

Method)

EF=dark brown

Nuclei=blue

Krajians

Technique

Congo red

EF=bright red

Fibrrin and connective tse=dark blueRBC=orange-yellow

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MSB TechniqueMartius yellow,brilliant

crystal scarlet,soluble blue

Nuclei=blue

Erythrocytes=yellow

Muscle=red

Collagen=blue

Fibrin=red

Mallorys(PTAH) Phosphotungstic acid

Fibrin,muscle

striations,neuroglia,amoebae=db

Nuclei,cilia,rbc=blue

Myelin=lighter blue

Collagen,osteoid,cartilage,ef=deep

brownish-red

Cyoplasm=pale pinkish brown

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Grams Iodine

Purple or blue

Highmans Congo red Congo red

Amyloid=deep pink-red;Nuclei=blue

Alkaline congo red congo red

Amyloid,ef,eosinophil granules=red

Nuclei=blue

Krajians

Amyloid(Modified

bennhold Method) Congo red

Amyloid=red;Ef=red but darker

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High pH Congo redtech(eastwood and

cole)Congo red

Amyloid,et,eosinophilic gran=redNuclei=blue

Methyl Violet-Crystal

VioletMethyl or crystal

violetAmyloid=purplish red

Nuclei,cytoplasm connective

tse=shades

Induced Fluorescent

Staining w/

Thioflavin-TThioflavin-T

UV=Silver-blue

Yellow

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Modified gomorisTrichome Mf=red;Collagen=greenNuclei=blue-black

Rapid gomoris

Trichome(fmt)

Myofibrils=greenIntermyofibrillar mtrl=bright red

PTAH Nuclei=deep blueFibrin=blue

Collagen=reddish

Coarse ef=bluish

Myelin=blue to blue-grayMuscle,neuroglia=blue

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L

A

B

S

C

I

E

N

T

I

S

T

Heidenhains ironhematoxylin Muscle

striation,mitohondria,myelin,chromatin=grey-black

Lissamine fast

red-tartrazine

methodNuclei=black

Muscles,RBC=red

Collagen=yellow

Schmorls Picro-

Thionin method

Lacunae and canaliculi=dark brown-black

Bone matrix=yellow or brownish- yellow

Cells=red

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Rapid toluidine-eosin

stain

Eosin and toluidine

N=blue

C=blue/pink

E.G=redBasophils and mast

cell granules=blue

Romanosky-Include

Jenner, Giemsa, May

Grunwald and Leishmanstains

Wrights & Leishmans

stains: modern

Main difference lies in the

manner & method of

polychroming methyleneblue

Methylene blue/azure

b and eosin

Wright

Methylene blue

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Giemsa-By itself: stains rbcand neutrophilgranu;es poorlyAzurophil granules red, stained wellInclusion bodies -excellent

Thioninnw/eosin andmethyleneblue

Azurophil granules red

Wright-giemsa N=purple/blue

C=pink/blue

E=pink/red

May-grunwald-

giemsa

Peroxidase reaction C=Green dark granule