Stable Isotope and Metabolomics Core Facility

AECOM Diabetes CenterThe Einstein Stable Isotope & Metabolomics Core is within the NIH funded Diabetes Research Center at Einstein and anchors the CMCR Metabolomics Core. The Core uses stable isotope flux and metabolite profiling to help formulate and test hypotheses about the metabolic consequences of various changes in gene expression and protein function, in order to guide further integrative systems biology analyses of the underlying mechanisms in diabetes, insulin resistance, obesity, and diabetic complications. The Core objectives includes assessments of plasma, urine and tissue metabolite profiles for diagnosis/characterization of physiological and pathophysiological states. These include key metabolites in the glycolytic, gluconeogenic, pentose phosphate, and tricarboxylic cycle pathways, fatty acid oxidation and complex lipids, such as ceramides, diacylgylcerols, triacylglycerols (TAGs), and phospholipids. In the CMCR framework, the Core will use these methodologies to explore the specific effects that radiation has on the metabolic network of each organ, and to explore the metabolic consequences of protein modification, by radiation, on the function of the metabolic network.

CMCR-AECOM

Stable Isotope & Metabolomics CoreLocation: Price 368

Director: Irwin Kurland,

MD PhDSandhya Narasimhan, PhD

Lab Members:

Albert Einstein College of MedicineOF YESHIVA UNIVERSITY

BhavapriyaVaitheesvaran, PhD

Kirsten Hartil, PhD

Hui-Min Zhang PhD

Metabolite Profiling and Flux Analyses

LC/MS Specialist

Seahorse Extracellular Flux Analyses

Mitochondrial PhysiologyAnimal Research Coordinator

XF Applications in Cellular Bioenergetic AssaysXF Applications in Cellular Bioenergetic Assays

MitochondrialFunction

In vitro ToxicityCancer

Fatty Acid Oxidation& Glycolysis

Cell quality

Neurodegenerativediseases

Translation to Clinical Applications

Cardiovascularresearch

Obesity/diabetes

XF96 ExtracellularXF96 Extracellular Flux AnalyzerFlux Analyzer

LC/MS AnalysisLC/MS Analysis

Simultaneous analysis of multiple metabolites

Waters AquityHigh resolution Chromatography

Identification of unknown species by structure elucidation

Multivariate Statistical Analyses

LTQ-Orbitrap

Waters Xevo Tandem MS

Synapt G2 Linear Hybrid quadrapole Time of Flight MS with MALDI

Synapt G2 itself Synapt G2 with MALDI front end

GC/MS Analysis of MetabolitesGC/MS Analysis of Metabolites

Lipid profiling: Fatty acids DAGs, TAGsCholesterol

Metabolites from theglycolytic pathway, TCA cycle, pentose cycle and amino acids

Stable isotope assessments of dynamic glucose disposal (SipGTT), lipogenesis ([2H]2O or U-13C]lactate), glucose production ([U-13C]glucose), glycerol production/lipolysis ([2-13C]glycerol), Cori cycle ([U-13C]lactate)

Waters Premier GC-Time of Flight-MS