st. marianna university school of medicineigakukai.marianna-u.ac.jp/idaishi/www/353/05-35-03manae...

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Page 1: St. Marianna University School of Medicineigakukai.marianna-u.ac.jp/idaishi/www/353/05-35-03Manae Kurokawa.pdfstem cells transfected with IGFII gene in a mouse model of muscle injury

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Page 2: St. Marianna University School of Medicineigakukai.marianna-u.ac.jp/idaishi/www/353/05-35-03Manae Kurokawa.pdfstem cells transfected with IGFII gene in a mouse model of muscle injury

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Page 3: St. Marianna University School of Medicineigakukai.marianna-u.ac.jp/idaishi/www/353/05-35-03Manae Kurokawa.pdfstem cells transfected with IGFII gene in a mouse model of muscle injury

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Page 4: St. Marianna University School of Medicineigakukai.marianna-u.ac.jp/idaishi/www/353/05-35-03Manae Kurokawa.pdfstem cells transfected with IGFII gene in a mouse model of muscle injury

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Page 5: St. Marianna University School of Medicineigakukai.marianna-u.ac.jp/idaishi/www/353/05-35-03Manae Kurokawa.pdfstem cells transfected with IGFII gene in a mouse model of muscle injury

���������24�� �� � �� �������������� ��������� !��� "�#�$%�&�� �'()*$+,-25�� .���/�&0��1 EphrinB2� EphB4 2�34�,� �� ��� 5,�670���/+,�82�����-9&� ��670����+,�0���:�;��<=� Tie2 1�ES 0�� >?@A@BCDE�0�� >?@A@BCDE�F OP90�2GH�&0�����34�,&� 670�I��J+,� PDGF1$%�&��K$�L��0�!MN��:O�PQ!�R�� 8� PDGF � B S�>?@A@BCDE�0�� >?@A@BCDE�F OP90�2GH�&0�T���34�,&� ���UV0��WCXC2-� aSMA ���1� OP90�!YZ[�0��\���34�,&� >?@A@BCDE��]^�_��0���/+,Q�82�`a+,�� bcde@���!f���T ghi!j9&�� >?@A@BCDE�0�� Flk-1���!34� >?@A@BCDE�F OP90�2GH�&0����670��WCXCTk� VE-cadherin� CD31����34�,&�� 2��8,��$l��� >?@A@BCDE�\mnOP9 0�2�GH�mo�p��q0�\mn��670���0���/TQ�82�`a+,&� rF8�0�s�R� +��tuvw�<O�x�yzt*I!;{O�2G�� 8,��0�!|}� ~1 3��H�mo��!*����������� �6T����*�vtu!���Q&� �� ES 0����/�&��0�v��V0�2G���@�����O�82�mo� ��������������� ������K$+,�����2��m'->X���T��O���� � ����de@(T���-�9&tu����M��mo�tu���O��!��O���Tk��

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.��8,(T�W�D!�&����� ES0���w�/�&���0�����8,(T��-����� ¡�¢���Tk�82�`+,&� 82���tuv��V�tu���1£ ���Tk9&� ���-��� ES 0��1¤

K¥��!1�4� �¦§�!�¨O©�� ªo«¬j�-­,®-�-¯°�±� ���-¯°��²�O�³��k��.�1´���¦§�!�¨�� rF�³�-µ¶���!·¸¹º)��Tk��

� �

1� Chiba S, Ikeda R, Kurokawa MS, YoshikawaH, Takeno M, Nagafuchi H, Tadokoro M,

Sekino H, Hashimoto T and Suzuki N. Ana-

tomical and functional recovery by embryonic

stem cell-derived neural tissue of a mouse

model of brain damage. J Neurol Sci 2004; 219:

107�117.2� Ikeda R, Kurokawa MS, Chiba S, YoshikawaH, Hashimoto T, Tadokoro M and Suzuki N.

Transplantation of motoneurons derived from

MASH1-transfected mouse ES cells reconsti-

tutes neural networks and improves motor

function in hemiplegic mice. Exp neurol 2004;

189: 280�292.3� Ikeda R, Kurokawa MS, Chiba S, YoshikawaH, Ide M, Tadokoro M, Nito S, Nakatsuji N,

Kondoh Y, Nagata K, Hashimoto T and

Suzuki N. Transplantation of neural cells de-

rived from retinoic acid-treated cynomolgus

monkey embryonic stem cells successfully im-

proved motor function of hemiplegic mice with

experimental brain injury. Neurobiol Dis 2005;

20: 38�48.4� Hamada M, Yoshikawa H, Ueda Y, Kuro-kawa MS, Watanabe K, Sakakibara M, Ta-

dokoro M, Akashi K, Aoki H and Suzuki N.

Introduction of the MASH1 gene into mouse

embryonic stem cells leads to di#erentiation of

motoneuron precursors lacking Nogo receptor

expression that can be applicable for transplan-

tation to spinal cord injury. Neurobiol Dis

2006; 22: 509�522.5� Kamochi H, Kurokawa MS, Yoshikawa H,Ueda Y, Masuda C, Takada E, Watanabe K,

Sakakibara M, Natuki Y, Kimura K, Beppu

M, Aoki H and Suzuki N. Transplantation of

myocyte precursors derived from embryonic

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stem cells transfected with IGFII gene in a

mouse model of muscle injury. Transplanta-

tion 2006; 82: 516�526.6� Homma R, Yoshikawa H, Takeno M, Kuro-kawa MS, Masuda C, Takada E, Tsubota K,

Ueno S and Suzuki N. Induction of epithelial

progenitors in vitro from mouse embryonic

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