SIVA RAMAKRISHNA

UPPALAPATI DOB: July 20th, 1985 Email: meetsivaram@gmail.com Ph: +91 9972856712, 9848893812 Personal Website: http://meetsivaram.wix.com/scientist-site

I AM

RECENT POSITION

Principal Investigator

Employed in Vins Bioproducts Ltd, Hyderabad on ‘quid pro quo’ basis contract (from March, 2014 to Sep, 2015) in ‘Equine Anti-Gas gangrene Antitoxin’ development.

OBJECTIVE

To be in an esteemed institution where work environment reinforces culture on a daily basis by encouraging its members to exercise organizational practices.

AWARDS

EDUCATION

BSc Hons.

In Biosciences Sri Sathya Sai University, Puttaparthi, India CGPA: 4.91/5.00

PhD

In Microbiology Bharathiar Univer-sity, Coimbatore & DFRL, Mysore

MSc Hons.

In Biosciences Sri Sathya Sai University, Puttaparthi, India CGPA: 5.00/5.00

Pre-University

Vikas Junior College Guntur, India 94.6%

SSC

Sarada High School Proddatur, India 87.5%

TIME LINE

1985

2015

2014

2013

2012

2011

2010

2009

2008

2007

2006

2005

2004

2003

2002

2001

Family re-location

JRF SRF

PI @ Vins Bio

Born

ABOUT ME • Full Name: Siva R Uppalapati

• Also called: Siva Ram • Father’s name: Jagan Mohan U Late • Mother’s name: Krishna Veni Late • Siblings: Sasank U, Wipro Software • Date of Birth: 20th JULY, 1985. • Address: Microbiology Dept, DFRL, Mysore, Karnataka, India • Nationality: Indian • Race: Asian • Languages: English, Telugu, Hindi • Health: Non-smoker, Non-alcoholic • Food habits: Veg & Non-veg • Veteran status: No • Sports: Table Tennis & Cricket • Hobbies: Medical & Science Fiction • Marital status: Unmarried • Status: In a relationship • Blood group: O +ve • Interests: Photography & Theatre

1/5

SIVA RAM

DRDO Technology Group Award for ‘Chimeric Gene Synthesis’ as the best technology innovation in DFRL

Best research paper award in TSICON 2013 International conference on “Biology of Natural Toxins” held at BITS, Pilani, KK Birla Goa campus, Goa

First prize in oral presentation in National Seminar on “Emerging Trends on Animal Biotechnology” held at Kakatiya University, Warangal, India.

Sri AV Gokak Gold medal in Masters

National 246th rank (98.6 percentile) in Graduate Aptitude Test in Engineering (GATE)

‘Council of Scientific and Industrial Research - Junior Research Fellowship JRF’ and ‘Senior RF’

2013

2013

2010

2009

2009

2009

Represented Vins Bioproducts Ltd, Hyderabad in TSICON 2014 held at Kolkata, India on ‘Development of Equine Anti-gas gangrene antitoxin’

2014

Selected for ‘Journey through Science day’ held by Pepsico and New York Academy of Sciences for presentation held in New York, USA, 2015.

2015

• Training classes on Molecular Biology and Developmental Biology to graduates and post graduates for national level competitive exams in Mysore University, India.

• Guest lectures delivered in JSS Women’s College, Mysore; MS Ramaiah Arts and Science College, Bangalore; Vignan University, Guntur, India on Therapy against Clostridial gas gangrene by Structural Vaccinology.

SIVA RAMAKRISHNA UPPALAPATI CURRICULUM VITAE

2/5

RESEARCH EXPERIENCE

Projects Involved Role

Real Time PCR detection of Salmonella Typhimurium from foods

• Standardization of qPCR by Taguchi optimization strategy. • Assay design and application on natural food samples.

Immunotherapeutics and immunodiagnostics against Anthrax

• Development of indirect Dot ELISA for detection of B. anthracis. • Development of an easy immunofluorescent assay for differential detection of

B. anthracis. • Design and development of low cost broad range epitope mapping strategy for

MoAbs.

Immunotherapeutics against Yersiniosis and plague.

• Study design and validation of experimental procedures on rationale behind design of a chimeric antigen encompassing domains of Y. pestis.

Virulence of Burkholderia species. • Functional and biochemical characterization of a novel toxin BLF1 elaborated by Burkholderia species.

• Structural analysis of BLF1 for development of a protective molecule.

Anthrax outbreak in Karnataka, India

• Core member of action team constituted by DRDO in response to an anthrax outbreak in animals.

Equine anti-gas gangrene antitoxin development

• Developed industrial scale C. septicum and C. novyi toxin production and extraction protocols.

• Developed immunization schedules in equines. • Product development in compliance with British and Indian pharmacopoeia.

Immuno assay based potency testing of Anti-snake venom serum products.

• Standardization and development of modified indirect ELISA based potency testing.

Mycotoxin diagnosis • Optimization of multiplex PCR for concurrent detection of major cereal associated mycotoxins.

• Development of Sandwich Dot ELISA for detection of Ochratoxin A from cereals.

Determination of microbial safety of Tender Coconut Water (TCW) product developed for Indian Army.

• Standardization and development of qPCR for assessment of B. cereus cells from TCW.

Generation and Characterization of a Bivalent Alpha Toxin Chimera from Clostridium perfringens and Staphylococcus aureus for Detection and Prophylactic Applications

PhD THESIS

Major achievements:

TEACHING EXPERIENCE

Heterologous protection against alpha toxins of C. perfringens and S. aureus induced by chimera in mouse model.

In silico, In vitro, in vivo evidence implicating stable interaction between N and C domains of C. per-fringens alpha toxin

A rapid, reliable and robust duplex PCR with IAC to detect alpha toxins of C. perfringens and S. aureus.

A Western blot and Dot ELISA system for differ-ential detec-tion of alpha toxins of C. per-fringens and S. aureus.

Rational design of a novel structural chimeric candi-date vaccine against C. perfrin-gens and S. aureus co-infections.

ARTICLES UNDER REVIEW • Litty Babu, SR Uppalapati, HS Murali and HV Batra.

Molecular diversity of drug resistant Klebsiella pneumoniae strains isolated from a hospital in Karnataka, India – Submitted to Microbial Research.

• Jaya Prahash C, SR Uppalapati and JJ Kingston. Thermal process validation of tender coconut water spiked with Bacillus cereus using RT-PCR technique – Submitted to Journal of Food Science and Technology.

• SR Uppalapati, JJ Kingston, HS Murali and HV Batra. Taguchi optimization of SYBR Green based duplex real-time PCR for concurrent detection of enterotoxigenic Clostridium perfringens and Listeria monocytogenes – Submitted to Food Microbiology.

• JJ Kingston, SR Uppalapati, Nisha M, HS Murali and HV Batra. Detection of Salmonella Typhimurium in food samples using Taqman-MGB probe based real-time PCR – Submitted to Food Microbiology.

• SR Uppalapati, JJ Kingston, HS Murali and HV Batra. Indirect immunofluroscence assay for detection of Bacillus anthracis spores and vegetative cells – Submitted to Current Science.

• Nirmaladevi D, M Venkataramana, SR Uppalapati, VK Gupta, Yli-Mattila T, Clement Tsui KM, Srinivas C and Chandranayak Maraka S (2015) Molecular phylogeny, pathogenicity and toxigenicity of Fusarium oxysporum f. sp. lycopersici. Scientific Reports (Accepted).

• R Rashmi, M Venkataramana, SR Uppalapati, et al., (2015) Development of sandwich dot-ELISA for specific detection of Ochratoxin A and its application on to contaminated cereal grains originating from India. Frontiers in Microbiology. Volume 6:511. Top ten most viewed article in May and June, 2015.

• Chitradevi STS, Kaur G, SR Uppalapati, et al., (2015) Co-administration of rIpaB domain of Shigella with rGroEL of S. Typhi enhances the immune responses and protective efficacy against Shigella infection. Cellular & Molecular Immunology. (Epub ahead).

• Shreya D, SR Uppalapati, JJ Kingston, et al., (2015) Immunization with recombinant bivalent chimera r-Cpae confers protection against alpha toxin and enterotoxin of Clostridium perfringens type A in murine model. Molecular Immunology. Volume 65 issue 1, pages 51-7.

• JJ Kingston, S Majumdar, SR Uppalapati, et al., (2014) Anthrax outbreak among cattle and its detection by Extractible Antigen 1 based Sandwich ELISA and immuno PCR. Indian Journal of Microbiology. (Published online).

• SR Uppalapati, JJ Kingston, HS Murali, HV Batra (2014) Heterologous protection against alpha toxins of Clostridium perfringens and Staphylococcus aureus induced by binding domain recombinant chimeric protein. Vaccine. Volume 32. Issue 25, pages 3075-3081.

• SR Uppalapati, JJ Kingston, HS Murali, HV Batra (2013) In Silico, In Vitro and In Vivo Analysis of Binding Affinity between N and C-Domains of Clostridium perfringens Alpha Toxin. PLoS ONE. Volume 8. Issue 12, e82024.

• SR Priyanka, SR Uppalapati, JJ Kingston, et al., (2013) Development of ISSR derived SCAR marker targeted PCR for identification of Aspergillus Section Flavi members. Letters in Applied Microbiology. Volume 58, pages 414-422.

• SR Uppalapati, JJ Kingston, HS Murali, HV Batra (2013) Taguchi Optimization of Duplex PCR for Simultaneous Identification of Staphylococcus aureus and Clostridium perfringens Alpha Toxins. FEMS Microbiology Letters Volume 340, Issue 2, pages 93–100.

• MS Shivakiran, JJ Kingston, SR Uppalapati, et al., (2013); Application of Extractable Antigen 1 (EA1) for specific detection of Bacillus anthracis cells. International Journal of Pharma and Bio Sciences Volume 4, Issue 2, pages 274–283.

• SR Uppalapati, JJ Kingston, HS Murali, HV Batra (2012) Generation and Characterization of an Inter-generic Bivalent Alpha Domain Fusion Protein αCS from Clostridium perfringens and Staphylococcus aureus for Concurrent Diagnosis and Therapeutic Applications. Journal of Applied Microbiology, Volume 113, Issue 2, pages 448–458.

• R Rashmi, MV Ramana, R Shylaja, SR Uppalapati, et al., (2012) Evaluation of a multiplex PCR assay for concurrent detection of four major mycotoxigenic fungi from foods. Journal of Applied Microbiology Volume 114, Issue 3, pages 819-27.

• Uppalapati SR and BS Vijayakumar (2009). Studies on aerobiology of Vidyagiri complex, Sri Sathya Sai University, Prasanthi Nilayam campus, Puttaparthi Mandal, Anantapur Dst, A.P. Biozone International J. Life Science 1(1):1-6.

SIVA RAMAKRISHNA UPPALAPATI CURRICULUM VITAE

3/5

ARTICLES PUBLISHED

• A monoclonal antibody specific for rEa1-C of Bacillus anthracis and a process for preparation thereof (2013). HV Batra, JJ Kingston, Shivakiran MS, SR Uppalapati, Radhika MU, HS Murali, U Tuteja and AS Bawa. Indian Patent file No. 2880/DEL/2011 A.

• A chimeric gene r-αCS encoding fusion protein composed of alpha toxins of Clostridium perfringens and Staphylococcus aureus. (2014) SR Uppalapati, JJ Kingston, HS Murali, HV Batra. Indian Patent file No. 726/DEL/2013.

• A chimeric gene r-cpae encoding fusion protein composed of alpha and enterotoxins of Clostridium perfringens (2014) Shreya D, SR Uppalapati, JJ Kingston, HS Murali, HV Batra. Indian Patent file No. 1918/DEL/2014.

PATENTS

0

10

20

ARTICLES CITATIONS PATENTS CONFERENCES

13

28

3

7

Cumulative I.F. – 33; Book chapters – 1; H- index - 3

PUBLICATION STATS

30

• Siva Ramakrishna Uppalapati and B.S. Vijaya Kumar., 2010. Aeromycological research in the new Millennium. A text book “Natural Resource Conservation and Environment Management” Ed: B. Behera, Siba Prasad Panda. A.P.H. Publishing Corporation, New Delhi. Page no: 274-282.

• U Siva Ramakrishna, S Jagdeesh Kumar, Vamsi Naveen Dheep, 2014. Managing the Mephitic Malady of Military: Development of Gas gangrene antitoxin. Presented at proceedings of The 4th Annual Conference and International Colloquium TSICon 2014 held during Nov 20-22, 2014 at Calcutta School of Tropical Medicine, Kolkata, India.

• U. Siva Ramakrishna, J.J. Kingston, H.S. Murali, H.V. Batra, 2013. Interactions between N and C domains of Clostridium perfringens Alpha toxin: In silico, in vitro and in vivo evidence. Presented at proceedings of First International Conference on Biology of Natural Toxins TSICon 2013 held during 19th – 21st Dec, 2013 at BITS, Pilani, KK Birla Goa campus, Goa, India.

• U Siva Ramakrishna & B S Vijayakumar, 2011. Studies on Aerobiology of Vidyagiri Complex, Sri Sathya Sai University, Prasanthi Nilayam Campus, (Gravity settle plate Method). at proceedings of National Conference on Impact of Biotechnology in Health Care and Industry (IBHI-2011) at Vel-Tech High Tech Engineering College, Chennai.

• U. Siva Ramakrishna, J.J. Kingston, H.S. Murali, H.V. Batra, 2010. Cloning, Expression and Purification of C and N domains of Clostridium perfringens. Presented at proceedings of National Seminar on “Emerging Trends on Animal Biotechnology” held during 26th – 27th March, 2010 at Kakatiya University, Warangal, India.

• U. Siva Ramakrishna, M.B.N.Chakravarthy, Robin Sharma & B.S. Vijayakumar, 2008. Studies on Aerobiology on Vidya Giri Complex, Sri Sathya Sai university, Prasanthi Nilayam Campus, Puttaparthi Mandal, Anantapur District, A.P. Presented at proceedings of National Symposium on the Biotechnology in Plant Disease Management for Sustainable Crop Protection organized by MACS’ Agharkar Research Institute, Pune and Indian Phytopathological Society, New Delhi (West Zone), September 17-18, 2008.

• M.B.N. Chakravarthy, U. Siva Ramakrishna, Robin Sharma, B.S. Vijayakumar and Ganesh Iyer, 2008. Studies on Semi-Solid fermentation of Drechslera puttaparthii sp.nov. and Testing its Enzyme Activity. Presented at proceedings of Leslie Coleman Memorial National Symposium on Plant Protection held during 4th - 6th Dec, 2008 at Department of Entymology, University of Agricultural Sciences, Gandhi Krishi Vigyan Kendra , Bangalore.

• U Siva Ramakrishna & B S Vijayakumar, 2008. Studies on Aerobiology of Vidyagiri Complex, Sri Sathya Sai University, Prasanthi Nilayam Campus, (Novel Aeroscope Method). Presented at the National Conference (XXI Annual Conference) on “Urban Environment – Present Scenario and Future Strategies held during December, 27th – 29th, 2008 organized by Department of Environmental Science, Bangalore University and National Environmental Science Academy (NESA), New Delhi, at Central College Campus, Bangalore University, Bangalore.

SIVA RAMAKRISHNA UPPALAPATI CURRICULUM VITAE

4/5

INTERESTS

Chimeric vaccine designing

Epidemiological studies of bacterial infections

Immuno & PCR diagnosis of microbes

Immunotherapeutics against cancer

Cancer Immunology - mechanisms

Bacterial & Viral infection

Cellular mechanisms involved in pathogenicity

Protein/Toxin structure analysis

Role of bacteria in Cancer

BOOK CHAPTER

CONFERENCES/SEMINARS

SKILLS

TECHNICAL

COMPUTER

MISCELLANEOUS

MOLECULAR TYPING

IMMUNO DIAGNOSTICS

VACCINOLOGY

SMALL ANIMAL HANDLING

PROTEOMICS eg. 2D GE

REAL TIME PCR

ANIMAL CELL CULTURE

BACTERIAL CLONING

STATISTICS

FACS

CN3D ANNOTATION

UPGMA/N-J DENDROGRAMS

PRIMER/PROBE DESIGNING

MICROSOFT POWERPOINT

MICROSOFT EXCEL

IMAGE ENHANCEMENT

CLUSTALW, BLAST

SURVIVAL STUDIES

CODON OPTIMIZATION

VIDEO MAKING & EDITING

LEADERSHIP

SOCIAL SERVICE

POETRY

PENCIL SKETCHING

VEDAM

TEACHING

PCR BASED DIAGNOSTICS: • A Taqman based Real-Time PCR assay for

detection of Salmonella Typhimurium using an MGB probe targeting a novel SNP in invJ locus.

• A SYBR green based Real-Time duplex PCR assay for detection of enterotoxigenic C. perfringens and Listeria monocytogenes from food samples and Bacillus cereus from tender coconut water.

• A rapid standardization method for PCR assays by Taguchi optimization strategy.

Siva Ramakrishna Uppalapati

5/5

SIVA RAMAKRISHNA UPPALAPATI CURRICULUM VITAE

ACHIEVEMENTS

Dr. M Venkata Ramana Principal Investigator R&D Vins Bioproducts Ltd, Hyderabad Ph: +91-781207333 Mail: ramana.micro@gmail.com

Dr. Harsh Vardhan Batra, Outstanding Scientist & Director, Defence Food Research Laboratory, Mysore, Karnataka, India Ph: +91-9342054402 Mail: drharshvardhanbatra@gmail.com

Dr. Joseph J Kingston Scientist D, Microbiology Division, Defence Food Research Laboratory, Mysore, Karnataka, India Ph: +91-9480292425 Mail: josephkingston@yahoo.com

REFERENCES

HARD-WORKING &

SKILLFUL

KNOWLEDGEABLE &

PRACTICAL

ORGANIZED &

FLEXIBLE

SUPPORTIVE &

CREATIVE

COMPETENCIES: CONVENTIONAL

& INNOVATIVE

I hereby declare that all the information above is correct and true to the best of my knowledge.

ANTIBODY BASED DIAGNOSTICS: • Indirect plate ELISA for detection of snake

venom from serum, rabies virus titers in patients and B. anthracis from food and blood samples.

• Indirect Dot ELISA for detection of B. anthracis, Ochratoxin A from food samples.

• Indirect Fluorescent-Antibody Assay for detection of Bacillus anthracis.

VACCINES & THERAPEUTICS: • Design & synthesis of novel chimeric candidate

vaccines against alpha toxins of C. perfringens & S. aureus; alpha & enterotoxins of C. perfringens.

• An equine trivalent antitoxin against alpha toxins of Clostridium perfringens, C. septicum and C. novyi responsible for Gas gangrene.

• Novel sub-unit candidate vaccine molecules against Burkholderia sps, Shigella sps and Staphylococcal enterotoxin B.

PRODUCTS DEVELOPED

ANTHRACHECK SAND-E Anthrax detection kit based on Indirect sandwich ELISA utilizing a novel in-house developed monoclonal antibody

GANGREVIN Anti-gas gangrene antitoxin of equine origin developed against lethal toxins of Clostridium perfringens, C. septicum & C. novyi

MOLECULAR TYPING: • RAPD and REP PCR analysis of C. perfringens

strains isolated from various areas around Mysore city, India.

• RAPD and REP PCR analysis of Penicillium strains isolated from food and feed products.

• ISSR microsatellite & ITS sequencing analysis of Fusarium oxysporum strains from contaminated Tomatoes from Andhra Pradesh, India.

• ISSR microsatellite analysis of Aspergillus section Flavi members isolated from cereals.

• Identification of section specific Sequence Characterized Amplified Region (SCAR) marker for identification of Aspergillus section Flavi.