sperm preparation, handling & storage · of innovative media solutions and lab supplies to the...

Solutions for the andrology laboratory

SPERM PREPARATION, HANDLING & STORAGE

Day 3

Day 1

Day 5

UNINTERRUP TED CULTURE

with simpler processes, less stress and better results

HELP AT EVERY STEP

TRANSFER BLASTOCYST

IVF

PREINCUBATE

RETRIEVE AND RINSE OOCYTES

PREINCUBATE WASH

ICSI

Vit Kit®-FreezeVit Kit®-Thaw

SEPARATESPERM

VITRIFICATION

DENUDE(ICSI)

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Multipurpose Handling Medium®

Continuous Single Culture® medium

Hyaluronidase Continuous Single Culture medium

Continuous Single Culture medium



Multipurpose Handling Medium

ISolate®



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Learn about the latest Irvine Scientific solutions for reproductive technologies in our brochures: - From gametes to blastocysts - Vitrification of oocytes and embryos - Sperm preparation, handling and storageVisit www.irvinesci.com

Irvine Scientific has been a well-recognized supplier of innovative media solutions and lab supplies to the A.R.T. community for almost 30 years.

SPERM PREPARATION, HANDLING AND STORAGE

· Maintain high quality, viable sperm

· Improve chance of fertilization

· Maximize survival rates

to greater workflow efficiency and increased pregnancy rates

LEADING THE WAY

Media solutions, such as ISolate® for sperm preparation and Freezing Medium TYB for cryopreservation, have set industry standards in the field of andrology. Irvine Scientific pioneered the use of vitrification to increase survival rates of cryopreserved oocytes and embryos with Vit Kit-Freeze and Vit Kit-Thaw.Today, with a new generation of optimized, multi-use media, Multipurpose Handling Medium® and Continuous Single Culture®, Irvine Scientific continues to increase workflow efficiency and contribute to successful pregnancies in clinics throughout the world.

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· Achieve high concentrations of progressive, motile spermatozoa

· Remove cellular debris, abnormal or immature sperm, seminal fluid, bacteria and lymphocytes

· Increase recovery rate and number of total motile sperm

PREPARE HIGH QUALITY SPERM FROM FRESH OR FROZEN SAMPLES

HIGH PERCENTAGE OF MORPHOLOGICALLY NORMAL FORMS

We use ISolate specifically to prepare frozen sperm samples.By using an improved method

(provided by Irvine Scientific) in combination with ISolate, we have

improved the yield of motile spermatozoa and so reduced the number of vials required for each treatment cycle.”

Charlotte Hall, Senior Embryologist, Sussex Downs Fertility Centre,

Eastbourne, U.K.

“

Leading commerical mediumISolate

Data courtesy of Dr. J. G. Mehta, Laboratory Director, Queen's Hospital, Romford, U.K.

25-30 36-4031-35 >40

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12.711 11

12.5

Mean Sperm Count Recovery (mil/mL) by Age Group

HIGHER RECOVERY

17.8 17.3 18

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ISolate Rapid one or two layer separations. Ready to use 50% and 90% gradients – no dilution required.

ISolate Stock SolutionRapid one layer separations. Ready-to-use, 90% gradients or dilute as required.

IMPROVE CHANCE OF FERTILIZATIONfrom any semen sample

ISolate is one of the most widely used media for isolation and purification of sperm prior to IUI, ICSI and IVF.

Well-established as a standard technique for separating healthy sperm, the concentrations of ISolate used in density gradient centrifugation can vary according to requirements for speed of preparation, level of purity or final sperm concentration. ISolate is available in a range of concentrations and sizes to meet the needs of every laboratory.

Select the ISolate product to meet requirements

ISolate ConcentrateDilute 100% isotonic solution as required.

SAVE TIME AND ENSURE CONSISTENCYPREPARE CUSTOM GRADIENTS

SAMPLES N=20 PRE-WASH ISolate COMMERCIAL ALT. 1

COMMERCIAL ALT. 2

Motility % 57.8 ± 9.1 81.4 ± 6.6 62.5 ± 13.2 85.7 ± 8.0

Total motile sperm (x106)

26.1 ± 16.7 15.8 ± 18.2 14.2 ± 15.9 10.6 ± 19.7

Recovery % - 48.9 45.3 30.8

DNA damage % 7.5 ± 9.2 7.2 ± 5.2 8.8 ± 4.7 11.2 ± 7.4

Semen quality parameters before and after separation by three different dual density gradients. (Malvezzi et al. Reproductive Biology and Endocrinology 2014 12:121).

HIGH RECOVERY OF MOTILE SPERM FROM HEALTHY AND LOW QUALITY SAMPLES

SAMPLE QUALITY PARAMETERS UNTREATED ISolate

Oligozoospermia (n=10)

Motile sperm recovery (%) - 32.9

Normal chromatin (%) 64.3 90.3

Normozoospermia (n=10)

Motile sperm recovery (%) - 44

Normal chromatin (%) 80.1 86.9

Good motile sperm recovery and normal chromatin condensation in healthy and low quality semen samples (Claassens et al. Hum. Reprod.1998 Nov;13(11):1998).

Since 1997, its use for density gradient centrifugation of semen samples has been referenced in more than 250 peer-reviewed publications. ISolate maintains physiological pH under ambient conditions by utilizing a colloidal suspension of silica particles stabilized with covalently-bound hydrophilic silane in HEPES-buffered HTF.

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One-step alternative to cryopreservation - enable semen storage off-site

· Preserve sperm viability during short-term storage at 2° to 8°C

· Store semen up to four days – no freezing, glycerol-free*

TYB PROTECTS SPERM DURING REFRIGERATION

SEMINAL FLUID: TYB 1:1, 4ºC

SEMINAL FLUID ONLY, 4ºC

Motility after 48 hours 29.6 + 21.5 6.2 +/- 9.2

*Baek et al. Can men produce and store semen at home? Poster presented at: ASRM 2006, Louisiana, USA P-1006

High number CASA analysis demonstrated significantly increased

parameters for CASA motility categories.

CE-marked TYB media was found to be a superior cryoprotective media than a glycerol-

only sperm freezing medium.”

Chey Dearing, Hammersmith Hospital, London UK

“

Refrigeration Medium TYB Simply add medium to semen and refrigerate.

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MAXIMIZE SPERM SURVIVAL

HIGHER MOTILITY THAN COMPETITOR'S MEDIUM

Hammadeh et al., J Androl. 2001 Nov–Dec;22(6):1012–8.

Increase recovery, maintain post-thaw viability

Freezing Medium TYB uses glycerol and the unique ability of TEST-yolk buffer (TYB) to protect sperm at low temperatures during long term storage. Freezing Medium TYB is the first and only CE-marked, FDA-cleared cryopreservation medium that contains egg yolk.

Use less medium to reduce costs and minimize dilution of samples

ArcticTM Sperm Cryopreservation Medium is a new TYB-free, dual-buffered medium designed for modern sperm processing. It is formulated to require less medium per application to save money and minimize dilution of frozen samples.

Maximize sperm survival rates after long- or short-term storage

· Maximize sperm survival from healthy or poor quality samples

HIGHER POST-THAW RECOVERY WITH IMPROVED QUALITY

FRESH POST-THAW

RAW SEMEN FREEZING MEDIUM TYB

COMMERCIAL MEDIUM, GLYCEROL ONLY

Poor quality semen (n=35)

Normal morphology (%)

11.7 ± 6.1 9.3 ± 5.6 7.8 ± 4.2

Condense chromatin (%)

78.9 ± 10.3 70.7 ± 10.8 64.6 ± 13.0

Motility (%) 28.3 ± 22.5 13.3 ± 9.9 10.7 ± 7.5

Healthy semen (n = 25)

Normal morphology (%)

26.3 ± 7.5 22.2 ± 6.4 19.4 ± 6.5

Condense chromatin (%)

92.4 ± 8.5 88.7 ± 11.2 85.5 ± 12.5

Motility (%) 52.8 ± 27.3 32.7 ± 19.1 29.1 ± 17.1

Freezing Medium TYB For cryopreservation, FDA-cleared.Ready-to-use.

Arctic Sperm Cryopreservation MediumConvenient kit size and refrigerated storage.Ready to use.

· Designed to maintain consistent pH before and after freezing with dual buffer base (HEPES/MOPS)

· Use 3:1 ratio of semen to medium · Improve post-thaw performance with the aid of key

antioxidants and amino acids

Supplier AArctic SCM

% M

otili

ty (T

est/

Cont

rol)

0 %

60 %

100 %

140 %

20 %40 %

80 %

120 %

160 %180 %

100 % 117 %Post Thaw Motility

100 % 117 %Post Gradient

Motility

100 % 127 %2 hr post Wash

Motility

Maintain high quality, viable sperm

MAINTAIN QUALITY

Work outside the incubator

Sperm Washing Medium is suitable for washing, swim up prep-aration, holding sperm prior to IUI and IVF, and other sperm-processing procedures performed outside of a CO2 incubator.

· Save time: ready-to-use, no mixing or filtering

· Maintain physiological pH 7.2–7.4 at ambient temperature

Process sperm in a safe environment

Multipurpose Handling Medium provides a versatile solution for swim up preparation, sperm washing, oocyte retrieval and rinsing, IUI, ICSI, and embryo transfer.

· Maintain physiological pH 7.2–7.4 and osmolality over a broad temperature range

· Support maintenance of cellular homeostasis

· Reduce risk of toxicity using a dual buffer system (HEPES and MOPS)

· Save time – no mixing or filtering, ready to use

Multipurpose Handling Medium – CompleteReady-to-use.

Multipurpose Handling MediumAdd preferred supplements.

Sperm Washing MediumAdd preferred antibiotics.

Multiple processes,one medium

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ORDERING INFORMATION

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ITEM CATALOG # SIZE ADDITION AL INFORMATION SHELF LIFE STORAGE

ISolate 99264 12 x 6 mL (up to 18 isolations)2 x 50 mL (up to 25 isolations)2 x 100 mL (up to 50 isolations)

Ready-to-use density gradient: 50% upper layer, 90% lower layer

2 years* 2–8°C

ISolate Stock Solution 99275 100 mL Ready-to-use or for dilution: 90% density gradient

2 years* 2–8°C

ISolate Concentrate 99306 100 mL 100% isotonic solution for custom dilution 2 years* 2–8°C

ITEM CATALOG # SIZE ADDITIONAL INFORMATION SHELF LIFE STORAGE

Freezing Medium TYB 90128 20 x 5 mL100 mL

Contains 20% egg yolk - from USDA certified SPF (Virus Free) laying flocks, heat inactivated at 56° C for 30 minutes, 12% (w/v) glycerol, gentamicin 10 µg/mL

2 years* -10°C


Arctic Sperm Cryopreservation-Medium

90170 12 x 5 mL Ready-to-use, egg yolk-free and antibiotic-free. Contains 28% (v/v) glycerol, 2% HSA

18 months* 2-8°C


Refrigeration Medium TYB 90129 20 x 5 mL Contains 20% egg yolk - from USDA certified SPF (Virus Free) laying flocks, heat inactivated at 56° C for 30 minutes, gentamicin 10 µg/mL

2 years* -10°C


Multipurpose Handling Medium – Complete (MHM-C)

90166 100 mL500 mL

Ready-to-use. Contains key amino acids, 0.5% HSA, gentamicin 10 mg/L.

12 months* 2–8°C

Multipurpose Handling Medium (MHM)

90163 100 mL500 mL

Contains gentamicin 10 mg/L. Add preferred supplements.

12 months* 2–8°C

Sperm Washing Medium 9983 12 x 12 mL100 mL

Ready-to-use outside the incubator. Contains 0.5% HSA. Add preferred antibiotics.

2 years* 2–8°C

*From date of manufacture

ORDERING INFORMATION


7% Polyvinylpyrrolidone (PVP) Solution with HSA

90121 5 x 0.5 mL Decrease sperm motility for easier micromanipulation during ICSI. Contains 5mg/mL HSA.

6 months* 2–8°C

10% Polyvinylpyrrolidone (PVP) Solution with HSA

90123 5 x 0.5 mL Decrease sperm motility for easier micromanipulation during ICSI. Contains 5mg/mL HSA.

6 months* 2–8°C

Polyvinylpyrrolidone (PVP) – Lyophilized

99219 10 x 1 mL Sterile, lyophilized powder for custom PVP concentrations. Must be reconstituted in Modified HTF Medium (90126).

2 years* 2–8°C

Modified HTF Medium 90126 100 mL500 mL

For handling sperm outside of an incubator. HEPES-buffered. Contains 10 mg/L gentamicin. Requires protein supplement.

12 months* 2–8°C

Human Serum Albumin Solution 9988 12 x 5 mL100 mL

Essential supplement to basal medium. Contains HSA 100 mg/mL in normal saline.

3 years* 2–8°C

Serum Substitute Supplement™ 99193 12 x 12 mL100 mL

Essential supplement to basal medium. Contains HSA 50 mg/mL, alpha and beta globulins 10 mg/mL.

2 years* 2–8°C

COUNTING AND MOTILITY EVALUATION OF UNDILUTED SPERM

SUPPLEMENTS AND MICROMANIPULATION SOLUTIONS

ITEM CATALOG # SIZE ADDITIONAL INFORMATION

Centrifuge IS-300 1 unit

NUNC 4 Well Dish N176740 120 dishes per case For IVF use.

Makler Counting Chamber Kit IS-363 1 unit Requires a standard microscope with 20X objective or 20X eyepiece.

Makler Counting Chamber – Chamber Grip

IS-363CG 1 unit

Makler Counting Chamber (Inverted) IS-365 1 unit Requires an inverted microscope.

Makler Counting Chamber (CASA) IS-373 1 unit Same as IS-363 but without grid.

Replacement Cover Glass (CASA) IS-374 1 unit Without grid. Compatible with IS-363 and IS-373.

Replacement Cover Glass IS-364 1 unit With grid. Compatible with IS-363.

Replacement Cover Glass (Inverted) IS-366 1 unit With grid. Compatible with IS-365.

*From date of manufacture

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Irvine Scientific was the first ART manufacturing company in the USA to receive ISO 13485:2003 quality system certification, the rigorous international quality assurance standard designed specifically for Medical Devices.

Products are manufactured in accordance with the Guidelines for Manufacture of Medical Device and In Vitro Diagnostic Products and the Good Manufacturing Practices (GMPs)

for Medical Devices.

Every Irvine Scientific product is subject to a stringent Quality System, unrivalled in the industry, and produced in well-established, cGMP compliant facilities.

Formulations use WFI Grade Water and chemicals that meet USP and ACS standards where available. All products are membrane-filtered and aseptically processed according to manufacturing procedures which have been validated to meet a sterility assurance level of 10-3. Every batch of medium is thoroughly tested for functionality, endotoxin level, pH, osmolality and sterility. Results are provided in lot-specific Certificates of Analysis.

With the exception of certain ancillary products, all products in this brochure are CE marked and FDA cleared. Quality and regulatory management systems ensure compliance with the broadest scope of regulatory requirements, including ISO 13485:2012, FDA’s QSR, European Medical Devices Directive (93/42/EEC).

at every step

PEACE OF MIND

Irvine Scientific®, the Irvine Scientific logo, ISolate®, Multipurpose Handling Medium® (MHM®), Serum Substitute Supplement™ (SSS™), Continuous Single Culture® , Arctic™ and Vit Kit® are trademarks of Irvine Scientific Sales Company, Inc.All other trademarks are the property of their respective owners.

© 2018 Irvine Scientific P/N 10646ART Rev.3

Irvine Scientific develops and supplies products and services wherever our expertise in the growth and handling of mammalian cells can contribute to the health and well-being of mankind – today and in the future.

Over 45 years of knowledge and experience

Innovative products, solutions and services

Unrivalled quality systems

www.irvinesci.com

sperm preparation, handling & storage · of innovative media solutions and lab supplies to the...

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