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Specimen Requirements
Heparin - green top EDTA - purple top Storage range 4°C = 2 - 8°C Room Temp = 18 - 25°C (Minimum volumes do not allow for retest) Rev 13JUL2017 | Page 1 of 4
For a complete test menu and specimen requirements please visit www.mplnet.com or call Client Services at 800.932.2943
Test Name (Test Code) Specimen(s) Volume (min.) Anticoag/ Medium Preferred (accepted)
Storage Stability Shipping Special Instructions
Flow CytometryWhole blood 4mL (1mL)
Bone marrow aspirate 2mL (0.5mL)
Sodium Heparin (EDTA)
BM core biopsy 2cm (1cm)
Fresh tissue 5mm3
FNA 4mL (0.5mL)
Sterile container, addition of 2-4mL transport media
Leukemia / myeloma / lymphoma analysis
Fluids, CSF, pleural, synovial, pericardial fluids
4mL (0.5mL) Sterile container, addition of 2-4mL transport media
Paroxysmal nocturnal hemoglobinuria; Leukocyte adhesion deficiency; ZAP-70
Whole blood 5mL (0.5mL) Sodium Heparin (EDTA)
Bronchoalveolar lavage lymphocyte subsets
Bronchoalveolar lavage 10mL (5mL) Sterile container, addition of 2-4mL transport media
4°C 48hrs Ice pack Protect from extreme temperatureSeparate ice pack from specimen
Pathology & ImmunohistochemistryAspirate 4mL (0.5mL) Sodium Heparin (flow and
cytogenetics; EDTA molecular studies)
RT 48 hrs Ambient
Core biopsy 1cm Formalin or B-Plus
Aspirate clot 5mm3 Formalin or B-Plus
Aspirate smear 4-6 slides Air dried
Bone marrow pathology evaluation(All specimen types required)
Blood smear 1 slide Air dried
RT Indefinite Ambient
Use hematopathology collection kit and include CBC report
Paraffin embed tissue Block Formalin fixedImmunohistochemistry markers
Slides; each marker/analyte – cut at 3μ and centrally located on “plus” slides
3 slides Air dried
RT Indefinite Ambient Protect from extreme temperature
Specimen Requirements
Heparin - green top EDTA - purple top Storage range 4°C = 2 - 8°C Room Temp = 18 - 25°C (Minimum volumes do not allow for retest) Rev 13JUL2017 | Page 2 of 4
For a complete test menu and specimen requirements please visit www.mplnet.com or call Client Services at 800.932.2943
Test Name (Test Code) Specimen(s) Volume (min.) Anticoag/ Medium Preferred (accepted)
Storage Stability Shipping Special Instructions
Molecular Oncology
Whole blood 5mL (3mL) EDTA or Sodium Heparin RT 72hrs AmbientHematological - NGSIgVH somatic hypermutationMyeloproliferative panelsCALR mutationJAK2 Exon 12 mutationMPL mutationMYD88 mutation
Bone marrow 3mL (1mL) EDTA or Sodium Heparin RT 72hrs Ambient
Paraffin embed tissue Block or 3-5 Slides 5um min
Formalin fixed RT Indefinite Ambient
Whole blood 5mL (3mL) EDTA or Sodium Heparin RT 72hrs Ambient
Solid Tumor- NGS
EGFR, KRAS, BRAF, IDH1/2 Genes
Colon, Lung, Melanoma, GIST panels
8 gene Solid Tumor panels
Bone marrow 3mL (1mL) EDTA or Sodium Heparin RT 72hrs Ambient
Protect from extreme temperature with ice pack.Do not allow the ice pack to directly contact the sample.Protect from extreme temperature with ice pack.Do not allow the ice pack to directly contact the sample.
Whole blood 5mL (3mL) EDTA or Sodium Heparin RT 72hrs Ambient
Bone marrow 3mL (1mL) EDTA or Sodium Heparin RT 72hrs Ambient
BM core biopsy 5mm3
Fresh tissue 5mm3
Fine needle aspirate 3mL (2mL)
4°C 72hrs Ice pack
Protect from extreme temperature with ice pack.Do not allow the ice pack to directly contact the sample.
Frozen tissue 5mm3
Sterile container, addition of 2-4mL transport media
-20°C Indefinite Dry ice Freeze immediately
PCR TestingB cell IgH heavy chain gene rearrangementB cell IgH kappa light chain gene rearrangementBCL2 t(14;18) gene rearrangement T cell receptor gamma gene rearrangement KIT (D816V) mutations
Paraffin embed tissue Block or 3-5 Slides 5um min
Formalin fixed RT Indefinite Ambient No heavy metal fixatives
Whole blood 5mL (3mL)KIT mutations AMLAML Mutation profile: FLT3, NPMI; CEBPA mutation, JAK2 V617F mutation
Bone Marrow 3mL (1mL)
EDTA or Sodium Heparin RT 72hrs Ambient
Whole blood 10mL min.ABL Kinase gene mutationBCR/ABL major p210 and minor p190 transcriptsPML-RARA t(15;17) short and long form
Bone marrow 2mL (1ml)
EDTA 4°C 48hrs Ambient
Protect from extreme temperature with ice pack.Do not allow the ice pack to directly contact the sample.
Specimen Requirements
Heparin - green top EDTA - purple top Storage range 4°C = 2 - 8°C Room Temp = 18 - 25°C (Minimum volumes do not allow for retest) Rev 13JUL2017 | Page 3 of 4
For a complete test menu and specimen requirements please visit www.mplnet.com or call Client Services at 800.932.2943
Test Name (Test Code) Specimen(s) Volume (min.) Anticoag/ Medium Preferred (accepted)
Storage Stability Shipping Special Instructions
Cytogenetics - OncologyWhole blood 7mL (5mL)
Bone marrow 3mL (1mL)
Sodium Heparin
BM core biopsy or fresh tissue
5mm3
Chromosome analysis
Fine needle aspirate 4mL (2mL)
Sterile container, addition of 2-4mL transport medium
RT 48hrs Ambient Protect from extreme temperature with ice pack.Do not allow the ice pack to directly contact the sample. For cancer cytogenetics please send WBC count with requisition. Call before sending specimen on Friday. Ship on Saturday for Monday delivery only.
Whole blood 5mL (3mL)
Bone marrow 3mL (1mL)
Sodium Heparin (EDTA) RT 72hrs Ambient
Fine needle aspirate 3mL (2mL)
Fluorescence in situ hybridization (FISH) probes for hematological disorders
Fresh Tissue 5mm3
Sterile container, addition of 2-4mL transport medium
4°C 72hrs Ice pack
BlockFluorescence in situ hybridization (FISH) probes for solid tumors
Paraffin embed tissue
3-4 Slides, 5 microns
Formalin fixed RT Indefinite Ambient
Protect from extreme temperature with ice pack.Do not allow the ice pack to directly contact the sample.
Bladder Cancer FISH Urine 50mL (35mL) PreservCyt® 4°C - 30°C 48hrs Ship overnight.
Molar pregnancy: 13+/18+/21+/X/Y
FFPE 3-4 Slides, Blocks Formalin fixed RT Indefinite Ambient Protect from extreme temperature
Cytogenetics - Constitutional
Newborn blood 1mL min Sodium Hep (ped tube)
Whole blood 7mL (5mL) Sodium Hep
Chromosome analysis
Umbilical blood 2mL (1mL) Sodium Hep
RT 48hrs Ambient
Solid tissue chromosome analysis
POC, chorionic villus, fetal tissue
Tissue, Skin biopsy
Sterile container, addition of 2-4mL transport media
RT 48hrs Ambient
Protect from extreme temperature. Call before sending specimen on Friday. Ship on Saturday for Monday delivery only if sample is protected from extreme temperature. Include gestational age and due date.
Specimen Requirements
Heparin - green top EDTA - purple top Storage range 4°C = 2 - 8°C Room Temp = 18 - 25°C (Minimum volumes do not allow for retest) Rev 13JUL2017 | Page 4 of 4
For a complete test menu and specimen requirements please visit www.mplnet.com or call Client Services at 800.932.2943
Test Name (Test Code) Specimen(s) Volume (min.) Anticoag/ Medium Preferred (accepted)
Storage Stability Shipping Special Instructions
Medical GeneticsRT 72hrs AmbientCystic fibrosis Luminex xTAG 39 Whole blood 5mL (3mL) EDTA
4°C 30 days Ice pack Protect from extreme temperature Separate ice pack from specimen
RT 72hrs AmbientCystic fibrosis MiSeqDx 139 Variant Assay by NGSCystic fibrosis MiSeqDx Clinical Sequence Assay by NGS
Whole blood 5mL (3mL) EDTA
4°C 30 days Ice pack Protect from extreme temperature Separate ice pack from specimen
Infectious DiseaseCervical cells 3mL ThinPrep® or SurePath™ 2-30ºC 21 days Ambient
Cervical or urethral swab One swab APTIMA Unisex Swab Specimen Collection
2-30ºC 60 days Ambient
Urine 20-30mL Preservative-free urine collection cup
4°C 24hrs Ice pack Void first 10mL
Chlamydia trachomatis Neisseria gonorrhoeae
Urine 2mL APTIMA urine tube RT 30 days Ambient
HPV high risk by AptimaTrichomonas Vaginalis
Cervical cells 2mL(1mL) ThinPrep® or SurePath™ RT 21 days Ambient
HPV genotyping Liquid cytology 2mL ThinPrep® or SurePath™ RT 21 days Ambient
HSV lesion viral transport media 4°C 96hrs Ice pack Separate ice pack from specimen
Whole blood 2mL EDTA 4°C 96hrs Ice pack Separate ice pack from specimen
Fresh Tissue 5mm3 4°C 96hrs Ice pack Separate ice pack from specimen
Frozen tissue 5mm3
Sterile container, addition of 2-4mL transport medium -20°C ~ Dry ice Freeze immediately
Swab from any site viral transport media 4°C 96hrs Ice pack Separate ice pack from specimen
4°C 72hrs Ice packCSF 2mL (1mL) Sterile container
-20°C ~ Dry ice Freeze within 4 hrs
Herpes Simplex Type I/2 qualitative by real-time PCR
Cervical cells 2mL (1mL) ThinPrep® or SurePath™ RT 21 days Ambient