soil ph and alfalfa cultivation

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    PERROTIS COLLEGE

    AMERICAN FARM SCHOOL, THESSALONIKI, GREECE

    (Degrees validated b

    CARDIFF METROPOLITAN !NI"ERSIT#, !K$

    Module title: %IOMETR#Module code:

    Module Leader: Dr& At'aasi)s Gertsis 

    Module instructor: Dr& "asilei)s ")*i+isStudent name: Eseia DeiaModule Level: -Semester: . / Fall 012340125Submission date:

    Research Project (100% of final grade-ma !ords"#000$ - ord count: 0657

    Title:Correlation between soil pH and plantdevelopment of alfalfa (Medicago sativa L)

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    Table of Contents&able of 'ontents)

    Summar* +

    ,ntroduction +

    Plant botan* descrition+'ro re.uirements/

    So!ing methods deth time and rate

    Pests and diseases2arvest3

    orld!ide roduction3

    4sage5

    6arieties10Stud* area 11

    Location11

    'limate117bjective(s$ 1)

    Materials and Methods1)

    Results: 1#

    Statistical evaluation of roject1+

    8iscussions:19

    Major 'onclusions: 19

    References 1/

    endi 1)0

    endi ))1

    endi #))

    endi +)#

    endi 9)+

    endi /)9

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    Summary

     Alfalfa  is one of the most useful and widely grown hay crops in the world, known from

    era of the ancient Greeks and Romans. However, its yields and quality of production are

    affected by different factors such as deficiency of one or more crop requirements, outbreak of

    diseases or/and pests, place and climate where it is sown. or this pro!ect we chose to analy"e

    and find if there is any correlation between soil acidity and plant development. #he e$periment

    was conducted on a field from %erpeni, Republic of &oldova. #he soil samples were brought at

    the 'errotis (ollege laboratory and analy"ed their acidity. )ata that resulted from our statistical

    analysis showed and proved our hypothesis that there is a high correlation between soil pH and

    plant development.

    Introduction

    *itrogen fertili"ation, organic matter decay, leaching, rainfall, and harvest of crops are

    the main factors of soil pH declines. &ost favorable pH for plant growth differs with crop species

    and with soil physical and chemical properties. +enefits of sustaining appropriate soil pH for

    crop production by spreading lime consist of improved * fi$ation by legumes, improved

    availability of essential nutrients -e.g., ', &o, and (a, increased percent base saturation, and

    reduced solubility of to$ic elements -e.g., Al and &n -(addel, . 0., 1hang, H., and 2ise, 3.

    4556.

    or this pro!ect we chose alfalfa crop as it is widely adapted and probably the most

    popular forage legume for hay and being also used in pasture for gra"ing. (addel and his

    coauthors stated in their work that decreased yields, shorten stand life of the crop, and reduced

    alfalfa competitiveness against weeds are the effects of low soil pH and nutrient deficiencies in

    acid soils. Alfalfa harvested as hay removes larger quantities of nutrients -see the Appendi$ 7,

    from soil than most other forage crops and corps harvested for grain -(addel, . 0., 1hang, H.,

    and 2ise, 3. 4556.

    Plant botany description

     Alfalfa generally grows four to eight years8 however it can grow more than 45 years,

    depending on variety and climate -Alfalfa 4579. #he plant matures to a height of up to 7 meter,

    and has a deep root system of two to four meters, sometimes e$tending more than 7: m -Alfalfa

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    4579. #he deep root system makes the plant to

    be very resistant, especially to droughts and

    frost. Alfalfa is a ;glabrous plant with alternate

    trifoliate leaves8 leaflets, 95 mm, narrowly

    obovate, toothed in upper third with a mucronate

    tip8 stipules linearcrown> at

    the top of the root system. #his crown has many

    shoot buds that allows alfalfa to regrow many

    times after being gra"ed or mowed -Alfalfa 4579.

    &oreover, ;crown is the central source of stems

    produced after defoliation8 a$illary buds above

    ground develop into branches. ?nflorescences are

    compact racemes up to 65 mm, borne in a$ils of upper leaves8 purple florets, @ mm, typically

    papilionacious. %eed pods spirally coiled,

    glabrous or pubescent8 pods turn from green to

    brown as they mature, and hold 4

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    an old alfalfa field rather than being rotated, or when alfalfa is sown into an e$isting alfalfa plot

    to thicken it -+agg . 4557. or that reason, alfalfa fields are suggested to be interchanged with

    other species -for e$ample, wheat or corn before reseeding -ennings . 4579. ?n the Appendi$

    9 is presented the difference between normal plant and the dwarf plant affected by to$ins.

    Crop reuirements

    For soil

      Alfalfa grows better on well

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    because their deficiency affects young tissue growth -+, and reduces the functioning of the

    nitrogenase en"yme in the *

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    $sage

     Alfalfa is very often mowed and harvested as hay, but may also be prepared into silage,

    gra"ed, or fed as green chop -(ertified

     Alfalfa %eed (ouncil ?nc. 4579. #he

    alfalfa field is not recommended to beused as pasture because it may provoke

    bloating in livestock -'lantde$ 4579.

     Alfalfa is considered ;3ing of

    odders= due to its high nutritional value

    -see the Appendi$ . ?t is used as food

    for high

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    -&edline 'lus 4579. ?ndirectly, alfalfa is a source of honey, because bees gather substantial

    quantities of nectar from alfalfa flowers.

    %arieties

    Flder varieties have been used for many decades8 however in the last few years

    appeared many better public and private varieties that are better adapted to particular climates

    -Rhodes 0. H., Enderwood .. D Henderlong '.R.4579.

    &ost varieties go dormant in the fall, this meaning that they have a reduced growth due

    to low temperatures and shorter days. >*ondormant> varieties that grow through the winter are

    cultivated in long

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    Study area

    Location As the study area

    we chose an alfalfa field

    which is situated in the

    south part and 75 meters

    above sea level in the

    village %erpeni, Republic

    of &oldova. #he

    coordinates of the field are

    6C.574@K* and 4.9:7@K

    -%tott . 4579. ?t is also

    situated appro$imately

    7755 meters away from

    the )niester River.

    Climate

    #he climate in the village is the same as the countryLs climate8 the only difference is the

    4

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    &b'ective(s)

    #he ob!ective of this paper is to find if there is any correlation between soil pH and plant

    development of alfalfa. ?t also proposes some treatments if there is any relation between soil

    acidity and plant development.

    Materials and Met!ods

    #he e$periment was conducted on a field of (herno"em soil -soil with lumpy or granular

    structure, high natural fertility, intensive and high humification, about 7:M content in the upper

    layers of humus with a mean initial pH ranging from :. to C.6

    #he field was divided into a grid of

    :5$74 meters from where were collected 76

    soil samples consisting of 7: cm. %oil

    samples were brought at the 'errotis

    (ollege laboratory where further analysis

    was conducted. %oil samples were oven<

    dried at :.:K( and ground to pass a 4

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    esults

    %oil pH 'lant height in cm

    C.9 4:

    C.9 46.:

    C.9 46.:C.6 4:

    C.6 4:

    C.4 47

    C.9 49

    C.9 49

    .: 7

    .: 7

    .@ 45

    :. 7C

    :. 7C

    7C.:#able 7

    ?n the table above, you can see all the collected data, from the soil analysis and plant

    height. ach number represents pH of the soil and the plant height in centimeters. +y careful

    observation you can notice that where the pH is higher the plant is higher.

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    Statistical evaluation of pro'ect

    Bivariate Fit of pH by plant height in cm

    Linear FitpH N 9.5C6:9 O 5.7C:5Pplant height in cm

    Summary of Fit R%quare 5.5945R%quare Ad! 5.@:76Root &ean %quare rror 5.75CC

    &ean of Response .@64@Fbservations -or %um 2gts 76

    Analysis of VarianceSource DF Sum of Squares Mean Square F atio&odel 7 6.5C:9: 6.5C:65 777.C:4rror 74 5.69C69 5.5965 !rob " F(. #otal 79 6.:74764 Q.5557P

    !arameter #stimates$erm #stimate St% #rror t atio !rob"&t&?ntercept 9.5C6:9 5.97C6@ @.:5 Q.5557Pplant height in cm 5.7C:5 5.57@: 75.:@ Q.5557P

    'orrelationVariable Mean St% Dev 'orrelation Signif( !rob )umber  plant height in cm 47.664 9.7C77C 5.:59C4 Q.5557P 76pH .@64@ 5.:@764

     An important thing is the correlation between soil pH and plant development.

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    Discussions

    ust having a simple look at the results we easily can observe the variance of plant

    height which is affected by the soil pH. Having the same look on the field we can see the

    difference that where the soil pH is higher the plants are higher, healthier, having more stems

    of a dark green color. Fn the other edge of the field where the soil pH is lower, the plants are

    dwarf having few stems of a light green to yellow color.

    ?n order to have a deep analysis and confirm our hypothesis we run the statistical

    analysis on &' 'ro 75 software. #he results showed above confirm our hypothesis that there

    is a high correlation between soil pH and plant development. As we can observe, the R %quare

    is relatively high and e$presses the 5M of the estimated correlation. Esing the bivariate normal

    ellipse of 'N5.5, we found that the plant height and soil pH are highly correlated8 the

    correlation being represented by :M.

    Major Conclusions:

    or this pro!ect we chose to analy"e and find if there is any correlation between soil

    acidity and plant development. #he e$periment was conducted on a field from %erpeni, Republic

    of &oldova. #he plant we chose is alfalfa, which  is one of the most useful and widely grown hay

    crops in the world, known from era of the ancient Greeks and Romans. #he soil samples were

    brought at the 'errotis (ollege laboratory and analy"ed their acidity. )ata that resulted from our

    statistical analysis showed and proved our hypothesis that there is a high correlation between

    soil pH and plant development.

    #his information might be useful to farmers who observed any difference of plant

    development on their fields. As recommendation, we propose to analy"e the field and the spot

    where the pH is low should be applied amendments such as liming, because the lime can

    correct the acidity.

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    eferences

    7. Alfalfa 4579, Britannica Online Encyclopedia, +ritanica.com, Retrieved *ovember 5@, 4579,

    ER0 Q http//www.britannica.com/+checked/topic/76::/alfalfa

    4. +agg . 4557,  Alala Autoto!icity"  &inistry of Agriculture and ood, Fntario, Retrieved

    )ecember 59, 4579, ER0 Q http//www.omafra.gov.on.ca/english/crops/field/autoto$.htm

    3. (addel, . 0., 1hang, H., and 2ise, 3. 4556, Responses o alala" red clover" and #hite clover

    to soil pH and lime treatments" orage and Gra"inglands, )ata viewed Fctober 4, 4579, ER0

    Q http//www.plantmanagementnetwork.org/pub/fg/research/4556/treat/  

    4. (alifornia Alfalfa 4579, Premium $uality Alala, Hay E%A ?nc., #e$as, E%A*  Retrieved

    *ovember 5@, 4579, ER0 Qhttp//www.hayusa.net/alfalfa.html

    5. (ash ). D Iuegao H. 455, Alala %anagement &uide or 'ing!ia" (nited 'ations Food and

     Agriculture Organization"  Retrieved *ovember 5@, 4579, ER0

    Qhttp//www.fao.org/ag/agp/AG'(/doc/ning$iaSguide/chapter7.pdf 

    6. (ash )., )itterline R. D )unn R. 79,  Alala )ariety *election, &ontana %tate Eniversity

    $tension, Retrieved *ovember 59, 4579, ER0

    Qhttp//msue$tension.org/publications/Agand*aturalResources/959AG.pdf 

    7. (ertified Alfalfa %eed (ouncil ?nc. 4579, Alala in the %outh, 2est Jirginia Eniversity, Retrieved

    *ovember 5@, 4579, ER0 Qhttp//www.caf.wvu.edu/Tforage/library/forglvst/bulletins/salfalfa.pdf 

    8. )i$on '. et. all. 455:, Establishing a *uccessul Alala +rop, &ontana %tate Eniversity

    $tension, Retrieved *ovember 5@, 4579, ER0

    Qhttp//msue$tension.org/publications/Agand*aturalResources/LJ:56AG.pdf 

    Page 2- of 0.

    http://www.britannica.com/EBchecked/topic/14595/alfalfahttp://www.britannica.com/EBchecked/topic/14595/alfalfahttp://www.omafra.gov.on.ca/english/crops/field/autotox.htmhttp://www.plantmanagementnetwork.org/pub/fg/research/2004/treat/http://www.plantmanagementnetwork.org/pub/fg/research/2004/treat/http://www.hayusa.net/alfalfa.htmlhttp://www.fao.org/ag/agp/AGPC/doc/ningxia_guide/chapter1.pdfhttp://msuextension.org/publications/AgandNaturalResources/MT199303AG.pdfhttp://www.caf.wvu.edu/~forage/library/forglvst/bulletins/salfalfa.pdfhttp://msuextension.org/publications/AgandNaturalResources/MT200504AG.pdfhttp://www.britannica.com/EBchecked/topic/14595/alfalfahttp://www.omafra.gov.on.ca/english/crops/field/autotox.htmhttp://www.plantmanagementnetwork.org/pub/fg/research/2004/treat/http://www.hayusa.net/alfalfa.htmlhttp://www.fao.org/ag/agp/AGPC/doc/ningxia_guide/chapter1.pdfhttp://msuextension.org/publications/AgandNaturalResources/MT199303AG.pdfhttp://www.caf.wvu.edu/~forage/library/forglvst/bulletins/salfalfa.pdfhttp://msuextension.org/publications/AgandNaturalResources/MT200504AG.pdf

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    . rame . 4579, %edicago sativa ,-"  AF, Retrieved )ecember 59, 4579, ER0

    Qhttp//www.fao.org/ag/agp/AG'(/doc/gbase/data/pf55596.htm

    10.Heu"e J. et. all. 4579, Alfalfa -&edicago sativa, eedipedia.org, ?*RA, (?RA), A1 and AF,Retrieved *ovember 5:, 4579, ER0 Q http//www.feedipedia.org/node/4C:

    77. Husman %.H. 4579, &ro#ing Alala or *eed in Arizona"  Eniversity of Ari"ona (ollege of

     Agriculture, Retrieved *ovember 5@, 4579, ER0 Qhttp//ag.ari"ona.edu/pubs/crops/a"774.pdf 

    12. ennings . 4579,  Alala or Dairy +attle"  Eniversity of Arkansas, )ivision of Agriculture,

    Retrieved *ovember 5@, 4579, ER0 Qhttp//www.uae$.edu/FtherSAreas/publications/')/%A<

    6555.pdf 

    79. ennings . 4579, (nderstanding Autoto!icity in Alala" Eniversity of Arkansas (ooperative

    $tension %ervice, Retrieved *ovember 5@, 4579, ER0

    Qhttp//www.uwe$.edu/ces/forage/wfc/proceedings4557/understandingSautoto$icitySinSalfalfa.ht

    m

     

    14. 0issbrant %. et. all. 4579, Phosphorus and Potassium Fertilization o Alala, 'urdue Eniversity,

    Retrieved *ovember 5@, 4579, ER0 Q http//www.e$tension.purdue.edu/e$tmedia/AI/AI

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    19. 'lantde$ 4579,  Alala .%edicago sativa/, Retrieved *ovember 5@, 4579, ER0

    Qhttp//www.plantde$.com/inde$.php/species

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    4C. 2orld 2eather and (limate ?nformation 4579,  Average #eather and climate in %oldova"

    Retrieved *ovember 5@, 4579, ER0 Qhttp//www.weather

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    *ppendi+ ,

    3ilograms of nutrient removed per ton of alfalfa produced, dry matter basis

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    *ppendi+ -

    'lant development within 95 days

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    *ppendi+ .

    ffect of autoto$icity on root development of alfalfa

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    *ppendi+ /

    !rincipal %iseases of alfalfa an% causal agentsDisease Agent

    +acterial leaf spot  6anthomonas alala

    (ommon leaf spot Pseudopeziza medicaginis

    Iellow leaf blotch ,eptotrochila medicaginis

    *temphylium *temphylium botryosum

    ,eptosphaerulina leaf spot ,eptosphaerulina briosianna5triolii 

    )owny mildew Peronospora trioliorum

    %pring black stem Phoma medicaginis var. medicaginis

     Alternaria Alternaria solani 

    +acterial stem blight Pseudomonas medicaginis or  syringae

    *tagonospora leaf spot *tagonospora meliloti 

    Rust (romyces striatus

    %ummer black stem +ercospora medicaginis%pring black stem Phoma medicaginis

    Fusarium wilt Fusarium o!ysporum

    )erticillium wilt )erticillium albo3atrum

    +acterial wilt +lavibacter michiganense subsp-insidiosum

    *clerotinia crown and stem rot *clerotinia trioliorum

    Rhi"octonia Rhizoctonia solani 

    Phytophthora root rot Phytophthora megasperma

     Anthracnose +olletotrichum triolii 

    Fusarium root rot Fusarium spp-

     Aphanomyces root rot  Aphanomyces euteiches

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    *ppendi+ 0

    Alfalfa pestsFoliage  

     Alfalfa weevils -larvae Hypera spp.

    (aterpillars +olias eurythene

    (utworms/army worms Eu!oa au!iliaris

    +lister beetles Epicauta spp.

     Aphids

    %potted alfalfa Therioaphis maculata5triolii 

    'ea  Acyrthosiphon pisum

    +lue alfalfa  Acyrthosiphon 7ondoi 

    (lover weevils *itona spp.

    Grasshoppers %elanophus spp.

    0eafhoppers  Agromyza rontella

    'otato leafhoppers Empoacea abae%pittlebugs -e.g. meadow Philaenus spumarius

    Roots/crowns  

    *ematodes

    Root knot %eloidogyne hapla

    Root lesion Pratylenchus penetrans

     Alfalfa stem Ditylenchus dipsaci 

    (lover

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    *ppendi+ 1

    Alfalfa see%s* sproute%* raw

    )utritional value per -.. g /0(1 o23

    nergy k -49 kcal(arbohydrates 4.7 g

    < )ietary fiber 7. g

    at 5.C g

    'rotein 6 g

    #hiamine -vit. +7 5.5C mg -CM

    Riboflavin -vit. +4 5.74 mg -77M

    *iacin -vit. +9 5.6@7 mg -9M

    'antothenic acid -+: 5.:9 mg -77M

    Jitamin + 5.596 mg -9M

    olate -vit. + 9 Vg -MJitamin ( @.4 mg -75M

    Jitamin 3 95.: Vg -4M

    (alcium 94 mg -9M

    ?ron 5. mg -CM

    &agnesium 4C mg -@M

    &anganese 5.7@@ mg -M

    'hosphorus C5 mg -75M

    'otassium C mg -4M

    %odium mg -5M

    1inc 5.4 mg -75M

    0ink to E%)A )atabase entry'ercentages are roughly appro$imated

    using E% recommendations for adults. 

    %ource  E%)A *utrient )atabase

    P 0. f 0.

    http://ndb.nal.usda.gov/ndb/search/list?qlookup=11001&format=Fullhttp://en.wikipedia.org/wiki/Dietary_Reference_Intakehttp://en.wikipedia.org/wiki/Dietary_Reference_Intakehttp://ndb.nal.usda.gov/ndb/search/listhttp://ndb.nal.usda.gov/ndb/search/listhttp://ndb.nal.usda.gov/ndb/search/list?qlookup=11001&format=Fullhttp://en.wikipedia.org/wiki/Dietary_Reference_Intakehttp://ndb.nal.usda.gov/ndb/search/list