sn ps
TRANSCRIPT
SNP GENOTYPING
Whitney Barfield and Daniel Salahuddin
Genotyping
Genotyping provides a measurement of the genetic variation between members of a species
SNP Example
PCR
Genotyping
The light emitted from the dye in the excited state is received by a computer.
Real-time PCR Machine PCR
The Read-Out
SNPSWhitney Barfield and Daniel Salahuddin
SNP SPP1
SPP1 binds and alters activity of a metalloproteinase
Metalloproteinase Family of genes
involved in adipose tissue remodeling
Linked to measures of obesity
Secreted Phosphoprotein 1 Obesity
SNP ATP2A1
ATPase, Ca++ transporting, cardiac muscle, fast twitch 1(ATP2A1) Involved in muscular
excitation and contraction
Brody disease (BD) inherited disorder of
skeletal muscle function Exercise induced symptoms
ATPase Clinical Symptoms
SNP MTCH2
Mtch2-resident complex participates in the mitochondrial apoptotic program.
Programmed cell death, or apoptosis is critical for both the
development and maintenance of tissues
Mitochondrial Carrier Homolog 2
(C. elegans)Caenorhabditis elegans