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June 18, 2022 June 18, 2022 Slit Lamp Examination - IC Slit Lamp Examination - IC 1 INSTRUCTION COURSE ON SLIT LAMP EXAMINATION Part II

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Page 1: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 11

INSTRUCTION COURSE ON SLIT

LAMP EXAMINATIONPart II

Page 2: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 22

M.P. STATE OPHTHALMIC CONFERENCE

UJJAIN

Page 3: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 33

Presented by Presented by

Dr. Sanjay ShrivastavaDr. Sanjay Shrivastava

Dr. Nikhilesh Trivedi Dr. Nikhilesh Trivedi

Dr. Kavita KumarDr. Kavita Kumar

Page 4: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 44

The science of examination with a slit lamp is called Biomicroscopy as it allows in vivo study of living tissues at high magnification.

Page 5: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 55

SET UPSET UP

Prerequisites – Preferably a dark room and slit lamp biomicroscope

Position – Patient is seated comfortably on an adjustable stool, with chin resting against chin rest and forehead against head rest bar.

Page 6: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 66

Examination of Various Ocular Structures

Page 7: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 77

1. LIDS

Diffuse Illumination Direct Focal Illumination

1. Hyperaemia – Capillary engorgement and suffusion of superficial vessels

Individual hair follicle

2. Diffuse inflammation (Edema) Meibomian gland orifice

3. Hordeolum externum Cilium

4. Tumours and Naevi Papillae on lid margin

5. Punctal Occlusion

6. Regurgitation on Pressure over sac

7. Mites and Parasites

Page 8: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 88

2. CONJUNCTIVA

- As the conjunctiva is transparent it is

mainly studied by light reflected from

sclera.

- Eversion of lid – Examination of upper

tarsal conjunctiva

- Double eversion of lid – Upper fornix

examination.

Page 9: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 99

(a) Diffuse Illumination

Following things are noted - General status of ocular surface.- Conjunctival congestion- Inflammation - Presence of foreign body- Tear Film assessment – Marginal tear meniscus

Page 10: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 1010

Diffuse Illumination

- Cysts- Concretions- Tumours- Nodules- Conjunctival pigmentation- Staining of tear film.- Staining of any conjunctival defect.

Page 11: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 1111

(b) Direct Focal Illumination :- Detailed examination of all lesions - Depth of any lesion.- Papillae- Follicles- Blood Vessels over a tumour - Neovascularization - Extent of subconjunctival haemorrhage

Page 12: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 1212

(c) Specular Reflection - Zones of intensely brilliant reflexes - Conjunctival elevated zones - Mucus and Waxy meibomian gland secretions- Elevated papillae

(d) Indirect Illumination :- Vessels- Conjunctival scars.

(e) Sclerotic Scatter Technique : - Pannus in Trachoma

Page 13: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 1313

3. CORNEA Diffuse Illumination

- Generalised view of surface of cornea

- Size

- Shape

- Transperancy

- Foreign Body

- Opacity

- Staining of Cornea

Page 14: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 1414

Direct Focal Illumination

Integrity of corneal epitheliumThickness of corneaDepth of lesionVascularisation-superficial/deepFolds in Descemet's membraneRupture in Descemet's mvmebrane

Page 15: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 1515

Hypopyon Ulcer seen in Direct Hypopyon Ulcer seen in Direct Focal IlluminationFocal Illumination

Page 16: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 1616

Direct Focal Illumination

Keratic precipitates can be seen in high powerOpacity in anterior part of cornea.DegenerationsDystrophies

Pigmentation KF ring Argyrosis Precorneal tear film

Page 17: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 1717

Specular Reflection

- Endothelial cell count (average 2800 cells/ Sq.mm)- Morphology of endothelium- Endothelial dystrophics- Hassall Henle bodies which are warts in descemet's membrane.- Bullae- Blood staining of endothelium

Page 18: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 1818

Sclerotic Scatter - Central nebular corneal opacity- Dystrophies- Corneal oedema- Rupture in descemet's membrane

Page 19: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 1919

Retro Illumination - Dystrophies of Descemet's membrane- Vacuoles- Keratic Precipitates (Stellate)- Oedema of epithelium- Bullae- Ghost Vessels

Page 20: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 2020

Corneal Staining

Fluorescein 2% Rose Bengal 1% Alcian Blue

Seen with cobalt blue filter Seen in red free filter Mucus

Corneal abrasion Mucus

Corneal erosion Keratin

Epithelial defect Abnormal and Devitalised epithelium

Ulcers Double staining

Siedel's test

Applanation tonometry

Break up time

Fit of contact lens

Page 21: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 2121

4. SCLERA AND EPISCLERA On Diffuse Illumination :- Deep, red, dusky congestion of episcleral vessels

On Direct Focal Illumination - Episcleritis – Raised congested nodule- Deep Scleritis – Conjunctival congestion associated with episcleral vessel congestion with peripheral keratitis and uveitis.

Page 22: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 2222

5. AQUEOUS HUMOUR Diffuse Illumination :- Depth of Anterior Chamber- Contents of Anterior Chamber- Foreign Body- Hypopyon- Hyphaema- Vitreous prolapse in AC- Traumatic Catractous material in AC- Micro Filareae- Cyst and Tumours

Page 23: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 2323

Direct Focal Illumination

- Attachment of pupillary membrane - Cells- Flare- Foreign bodies- Depth of Anterior Chamber

Page 24: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 2424

AQUEOS FLARE

Flare is the ability to see the path of light when slit beam is obliquely aimed across the AC. It is due to light scattering by suspended colloid particles causing TYNDALL effect.

Page 25: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 2525

AQUEOS FLARE

It is seen in 2 mm x 1 mm slit beam with maximum light intensity.

- It is seen against the iris plane.

- It can be seen in conical beam by direct focal illumination technique.

Page 26: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 2626

Grading of flareGrading of flare

0 Absent

1+ Faint Barely detectable

2+ Moderate Iris and lens details clear

3+ Marked, iris and lens details hazy

4+ Intense fibrinous aqueous

Page 27: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 2727

Cells :Indication of active inflammation of iris

and ciliary body.Slit beam is directed across the AC and

beam is focused posterior to cornea. 2 x 1 mm slit beam is focused with

maximum light intensity.0 - 01+ - 5 – 10 2+ - 10 – 20 3+ - 20 – 504+ - > 50

Page 28: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 2828

ANTERIOR CHAMBERVan Herrick Method :

For assessment of peripheral anterior chamber depth using a slit lamp.

A comparison of depth of peripheral anterior chamber to the peripheral corneal thickness is used to determined the degree of shallowness of the anterior chamber.

Page 29: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 2929

ANTERIOR CHAMBER

An optical section of peripheral cornea and anterior chamber is made on slit lamp with illumination and viewing arms at 60° to each other.

Viewing arm is perpendicular to cornea. Magnification is 15 X.

Page 30: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 3030

GONIOSCOPY It is done to see the structures in the

recess of angle of anterior chamber.It is important in - Glaucoma- Foreign bodies entangled in angle of AC- Tumours arising in angle of AC.- Narrowing of angle can be identified by

a steep configuration of iris and angulation of slit light reflex as it passes into the angle recess.

Page 31: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 3131

6. IRIS Diffuse Illumination:

Carried under low magnification :

- Colour of Iris :

- Heterochromia iridium

- Heterochromia iridis

- Gross structural abnormality

- Iris Motility

Page 32: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 3232

Direct Focal Illumination - High Illumination- Light beam focused from temporal side- First periphery is inspected then pupillary zone to avoid dazzling.Observation :- Exudates- Vascular changes- Atrophic Changes- Neoplastic ChangesOptical Section :- Narrow beam is made to see details of crypts and furrows.

Page 33: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 3333

Retro Illumination

- Visualization of iris is via light

reflected from the lens

- Atrophic patches in iris.

- Holes

- Tears and dehiscences.

- Patency of Peripheral iridectomy

Page 34: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 3434

Indirect Illumination - Outline of Crypts- Atrophic areas - Perforations - Cysts and Tumours- Haemorrhages

Page 35: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 3535

Iris Scatter

- Beam is focused on corneoscleral junction.

- Magnification is kept low or medium.- Used to see –- Iris contour, depression, discontinuities- Any tumour- Holes and other defects

Page 36: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 3636

7. LENS

Diffuse Illumination :

- To visualize anterior lens surface with

part of its anterior capsule.

- Surface and suture system of adult

nucleus in older patients.

- Embryonic Y suture.

- Posterior Capsule when altered

pathologically

Page 37: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 3737

Direct Focal Illumination :

- Enables to see zone of discontinuity

- Finer details of tissue stratification

- Exact localization of minute changes.

- Differentiate between congenital and

developmental opacities, senile changes,

traumatic opacities, complicated opacities.

Page 38: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 3838

Lens under direct focal illuminationLens under direct focal illumination

Page 39: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 3939

Retro Illumination :1. Used to observe whether the opacity is –

- Obstructive – Opaque to light- Respersive – Scatters light- Refractile – Refracts, minimizes or

distorts the views of background.2. Observe anterior capsular changes

- Deposits, Foreign bodies, Opacities Below Capsule.

Page 40: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 4040

Examination of LensExamination of Lens

3. Direct Retro Illumination – Obstructive Lesion Indirect Retro Illumination – Refractile

and Respersive

Page 41: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 4141

Posterior Sub Capsular Cataract Seen in Direct Focal Illumination and Retro Illumination

Page 42: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 4242

Specular Reflection :-

Mirror reflexes of anterior and posterior

capsule can be seen as bright reflex, when

the beam is moved from side to side across

the surface of lens. It is due to irregular

reflection caused by small irregularities of

capsular surface known as SHAGREEN

reflex. Any capsular opacity can be seen by

this method.

Page 43: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 4343

Post Operative Evaluation of IOL Surgery :

- IOL Centration and Stability.

- Position of IOL

- Pupillary Capture

- Dislocated IOL

- Early Uveitis

- PCO :

- Soemmerring Ring

- Elschnigs Pears.

Page 44: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 4444

8.PUPIL Diffuse Illumination

SizeShapeContourPupillary Membranes

Page 45: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 4545

Examination of PupilExamination of Pupil

Direct Focal Illumination - Direct Reflex - Consensual reflex- Accomodation reflex- Neovascularisation at pupillary margin - Exfoliative changes.

Page 46: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 4646

9. VITREOUS

- Examination of Anterior 1/3rd vitreous can be made using direct focal illumination.

- Illumination is kept maximum

- Slit very narrow.

- Illumination is brought from largest angle possible, without disturbing the slit.

- It is seen as optically clear space with delicate undulating fibrils and membrane like structures.

Page 47: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 4747

Direct Focal Illumination :- Shape- Rigidity- Vitreo retinal attachments- Vitreous opacities- Vitreous Bands and Membranes- Any pigmented Debris.- Foreign bodies in vitreous- Haemorrhages in vitreous

Page 48: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 4848

Slit lamp biomicroscopy can be used is combination with various lenses and mirrors for a magnified stereoscopic evaluation of retina and vitreous by vertical tilting of a slit 10° - 20°.

Page 49: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 4949

Goldmann Three Mirror Lens Examination :

- Central planoconcave lens – 64 D.

- It has 3 mirrors at 59°, 67° & 73°.

- Central lens allows a magnified stereoscopic examination of central 30° of retina giving an erect image.

- Oblong mirror gives a view of posterior retina.

- Rectangular and anterior retinal mirrors examine corresponding retinal areas respectively.

Page 50: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 5050

Indirect Slit Lamp Biomicroscopy :- Carried out by hand held high plus condensing lenses.- + 90 D, + 78 D.- An inverted, stereo magnified image is formed between lens and slit lamp.- Lens is held 5 – 10 mm from patient's cornea. - Magnification is 10 X or 16 X.- Coaxial illumination for fundus examination.- Slit beam is angled 10° – 20° from axis of observation for vitreous examination.

Page 51: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 5151

Hruby Lens Biomicroscopy :

- Planoconcave – high minus lens (–55D)

- Neutralises the optical power of the eye.

- Forms a virtual erect image of fundus.

Page 52: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 5252

Auxillary Devices :These can be used along with slit lamp.

1. Gonioscope for angle structure examination.2. Applanation tonometer for IOP.3. Endothelial cell counts using Eisner grid.4. BUT5. Staining Procedures.

Page 53: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 5353

Auxillary Devices

6. Corneal Thickness – Pachymetry7. AC Depth – Van Hericks Method.8. Ophthalmodynamometry – Pulsations of

blood vessels. 9. Slit Lamp Photography10. Delivery of Argon, Diode and Nd YAG laser 11. Laser Interferometry12. Potential Acuity Meter Test.

Page 54: Sl exam pt ii

April 11, 2023April 11, 2023 Slit Lamp Examination - ICSlit Lamp Examination - IC 5454

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