Skin Care-Anti-ageingSkin Care-Anti-ageing

Low carb meals &snacksLow carb meals &snacks

Healthy JointsHealthy Joints

Energy-Sport nutritionEnergy-Sport nutrition

Hair care productsHair care products

®

1000®

4000

Part I. What is NatiColPart I. What is NatiCol®®??

The Weishardt company introduces,NatiCol® 1000 and NatiCol® 4000, a complete range of marine hydrolyzedcollagen

NatiCol® is selectively derived fromfish skin & scales which have beenconsumed by mankind for a long time.

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Part I. NatiColPart I. NatiCol®® Flow chart Flow chart

Weishardt International is certified

ISO 9001/2000

Concentration

Degreasing

Purification

Enzymatic hydrolysis

Sterilisation

Extraction

Acid treatment

Filtration

Spray-drying

100% Fish & Scales skin

®

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Part I. NatiColPart I. NatiCol®® Traceability Traceability

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Integrating the fish activity from beginning to end, Weishardt controls each step. All raw material is veterinary controlled.

Weishardt controls each step and can guarantee total traceability from the farm to the delivered batch.

Part I. NatiColPart I. NatiCol®® safety safety

Applying gelatine process affords the best security.

2 sterilisations assure the best bacteriology.

Ionic demineralisation and filtrations the maximum purity and highest collagen ratio.

Weishardt, while purchasing various species worldwide guarantees the best long term security, for you and your customers.

Choosing NatiColNatiCol®® means also avoiding some risks and questions

regarding BSE for bovine collagen, avian flu for chicken collagen or any epizootic disease which could appear localy.

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Part I. NatiColPart I. NatiCol®® composition? composition?

NatiCol® contains:

・　 93-95% protein mainly collagen peptides type I.・　 Less than 6% water・　 Less than 2% mineral salts

NatiCol® is:

Carbohydrate FREE Fat FREE PURINE FREE CHOLESTEROL FREE OGM FREE PRESERVATIVE FREE

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Part I. NatiColPart I. NatiCol®® molecular weight molecular weight

Weishardt has developed two molecular weights covering the main market needs and adaped to various applications.

NatiCol® 4000 is close to usual Marine collagen found on the market (Av<4000Da). Its very high organoleptic properties make Naticol 4000 one of the most appreciated and make the difference!

NatiCol® 1000 is obtained through a special process in order to obtain low molecular weight short chains of peptides (Av<2000Da)It is particularly adapted to topic applications. Due to its faster assimilation by organism, it can be also adapted to some oral applications.

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Part I. NatiColPart I. NatiCol®®

1000 1000 specificityspecificity

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Molecular weight Distribution (MWD)

0,3-1 Kda minimum 40% 1-2 Kda 30%

NatiCol® has an extraordinary level oflow molecular weight which makes it so

unique compared to conventional collagen

®Brand X

MWD (Kda)

0,3-1 Kda3-5 Kda

®

1000

4000

Part I. NatiColPart I. NatiCol®® Amino-acid (AA) compositionAmino-acid (AA) composition

NatiCol® contains:

・　 27.6% of Glycine・　 23% of Proline & Hydroxyproline

which are the main components of skin

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0 5 10 15 20 25 30

Glycine

Proline

Hydroxyproline

Ac. glutamique

Ac. aspart ique

Alanine

Arginine

Serine

Histidine

Hydroxylisine

Tyrosine

NatiCol® contains:

8 essentials amino- acids which represents

14,5% of the total AA

0 0,5 1 1,5 2 2,5 3 3,5

Isoleucine

Leucine

Lysine

Methionine

Phenylalanine

Tréonine

Tryptophane

Valine

Esse

ntia

l AA

(%)

AA

(%)

85.5%

14.5%

Part I. NatiColPart I. NatiCol®® Amino-acid (AA) compositionAmino-acid (AA) composition

CHEMICAL INDEX ( way to look for the ideal protein) Naticol index is 146 bearing in mind that above 100 means complete

protein. Only the absence of Tryptophane is a limiting factor as for all collagens.

The index of amino-acids (Pro, Hypro, Gly) necessary for skin is double that of soy or milk protein.

0,00 5,00 10,00 15,00 20,00 25,00 30,00

Aspartic acid

Thréonine

Sérine

Glutamic acid

Proline

Hydroxyproline

Glycine

Alanine

Valine

Cystéine

Méthionine

Isoleucine

Leucine

Phénylalanine

Tyrosine

Lysine

Hydroxylisine

Histidine

Arginine

Scales

NaticolAA composition varies slightly according to age, food and origin.

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I. NatiColI. NatiCol®® solubility solubility

100% Natural Fish Collagen 2 types: Powder (Pd) & Micro-granulated (MG) White powder, Odourless

Excellent solubility in cold water (5%) Highly dispersible (up to 30% of MG) Very good clarity

Brand X®

®

Part I. NatiColPart I. NatiCol®® Key benefits Key benefits

■ 　 100% Natural Marine product■ 　 2 types: Powder & Micro-granulated■ 　 93 to 95% of pure Collagen■ 　 High level of oligo-peptides ■ 　 High level of essential amino-acids■ 　 Neutral taste and odour■ 　 Excellent solubility in cold water■ 　 Halaal certified

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NatiCol is a natural source of collagen type I, the

major protein found in connective tissues and

skin (more than 75%) which plays an essential

role in maintaining skin tone, suppleness &

elasticity. Unfortunately after the age of 25

collagen synthesis reduces at a rate of 1,5% a

year. By the age of 45 collagen levels can have

fallen by as much as 30% 1,2,3.

The external effects of this process are the appearance of wrinkles, fine lines & dry skin

Part II. NatiColPart II. NatiCol®® Skin benefits Skin benefits

Epidermis

Dermis

Hypodermis

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Part II. NatiColPart II. NatiCol®® Skin benefits Skin benefits

• NatiCol provides a pure & concentrated level of marine collagen (95%). Used in rejenuvating formulaes it help to replenish the body’s own

collagen and provides essential nutrients to re-build collagen cells due to its small molecule (tripeptides) and its high bioavailability.

• Regular use of products integrating NatiCol marine collagen may help slow the signs of

ageing by increasing Collagen synthesis, skin elasticity & firmness and reduce fine lines and

wrinkles .

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Part II. NatiColPart II. NatiCol®® 　　　　 Skin care markets & Skin care markets & applicationsapplications

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1. Topical cosmetic products 2. Oral cosmetic products

Skin care markets

Skin Anti-ageing Anti-wrinkle effect

Reduce fine lines Skin firmness Skin elasticity

Increase collagen synthesis

Applications

Part II. NatiColPart II. NatiCol®® Cosmetic use Cosmetic use

COSMETIC USE

DOSAGE/FREQUENCY

SAFETY/TOLERANCE

• NatiCol® can be used in topical cosmetic anti-age and skin care preparations (cream, lotions, emulsions).

• NatiCol® can be used in oral cosmetic «Cosmetofood» anti-age and skin care preparations (tablets, soft gels, beauty drink..)

NatiCol® is 100% safe and has a good tolerance.

1. Topical Dosage: 0,2 to 2%2. Oral Dosage: 5g per day4. Frequency of use: Daily or twice daily for 2-4 months3. Solubility : NatiCol is soluble in water, by stirring at room temperature.

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Part II. NatiColPart II. NatiCol®® Cosmetofood suggested formulationsCosmetofood suggested formulations

Example 2: Cosmetic supplements (sticks) for instant drinks

•NatiCol® (marine Collagen) : 800mg• Acerola extract (vit C) : 100mg• CoQ10 : 10 mg• Marine Chondroitin sulfate : 100 mg• Flavour

Example 1: Cosmetic supplements (tablets, capsules) for anti-wrinkle and anti-ageing effect

•NatiCol® (marine Collagen) :1000mg•Vitamin C : 100mg•Vitamin A (retinol) : 50 mg• OPC (grape seed extract) : 50 mg

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Part II. NatiColPart II. NatiCol®® Cosmetofood suggested formulationsCosmetofood suggested formulations

Example 3: Cosmetic Supplements for Skin Whitening & Anti-ageing effect

•NatiCol® (marine Collagen) :800mg•Fytoperfect (skin whitening complex) : 800mg•Vitamin C : 100 mg•Vitamin E : 100 mg

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Part II. NatiColPart II. NatiCol®®

　　　　　　　　　　　　 Cosmetic suggested Cosmetic suggested formulationsformulations

Example 5: Cosmetic cream for anti-wrinkle & moisturizing effect

• NatiCol® (95% pure marine Collagen) : 2%• Other ingredients: Vitamin E, Soy& Advocate oil, Glycerin, perfume, sodium sulfate, preservatives….

Example 4: Cosmetic gel for anti-wrinkle effect

• NatiCol® (95% pure marine Collagen) : 2%• Other ingredients: Carpobol , Glycerin, Perfume, sodium sulfate,

ethanolamin, preservatives

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Part II. BibliographyPart II. Bibliography

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• Babraj JA et al 2005: Collagen synthesis in human musculoskeletal tisues and skin. Am J. Ph. Endocrinol Metab. 289 (5) : 864-9.

• Fligiel SE et al (2003): Collagen degradation in aged/phodamaged skin in vivo and after exposure to matrix metalloproteinase-1 in vitro. J. Invest. Dermatol. 120, (5) 842-8.

• Chung JH (2001): Modulation of skin collagen metabolism in aged and photoaged human skin in vivo. J. Invest. Dermatol. 117, (5): 1218-24.

• Reuther T et al (2004): Active ingredients in dermatocosmetics. Hautartz.. 55 (7) :630-6.

Part III. NatiColPart III. NatiCol : Function in : Function in healthy jointshealthy joints

Both ends of the bone (epiphysis) -covered by the

articular cartilage- are kept in position by the joint capsule.

The synovial membrane binds the 2 articular surfaces and

secretes a synovial fluid which ensures cartilage lubrification.

Epiphysis

Articular cartilage

surface

Joint capsule

Synovial membrane

Epiphysis

Part III. NatiCol- Normal Articular Part III. NatiCol- Normal Articular CartilageCartilage

•Articular cartilage is a connective tissue made of cellular components (Chondrocytes) and an extracellular matrix , formed by collagen fibres and a gel called fundamental substance.

•This « fundamental substance » contains free proteoglycanes and free glycoproteines..

•Proteoglucanes are large molecules made up of a long chain of hyaluronic acid bound with protein and GlycoAminoGlycans (mainly Chonroitin sulfate).

Chondrocytes(Joint cells)

Extracellular matrix

Part III. What are Chondrocytes?Part III. What are Chondrocytes?

Chondrocytes are cells that help cartilage to grow and stay healthy.

They synthesize the collagen and proteoglucanes that form the extracellular matrix, the basis of

the cartilage tissue

Part III. What are GAGs?Part III. What are GAGs?

GlycosAminoGlycanes(GAGs)-previously called mucopolysaccharides-are large molecules formed by the

repetition of more than 100 polyssacharide units.

Chondroitin sulfate(CS) is the main GAG found in the articular cartilage.

CS is a glucoside sulfate polymer (50-100 Kda) formed by the repetition of dissacharide units of galactosamine and

glycuronic acid.

CS has a strong electronegative structure which confers a high hydrophobic capacity.

Part III. What is Collagen?Part III. What is Collagen?

•Collagen is an essential and major component of connective tissues (joints, tendons, cartilage, ligaments, blood vessels), and makes up 25% of the total protein mass.

•It is a complex trimer structural protein with a high level of glycine and proline and morever contains Hydroxyproline and Hydroxylysine found in very few other proteins

• There are 3 main types of collagen:

•Type I and III (found mainly in skin, tendon and bone)

•Type II (found exclusively in cartilage)

Part III. NatiColPart III. NatiCol

Healthy Joints markets & Healthy Joints markets & applicationsapplications

1. Senior Joint Health 2. Sportsmen Joint health 3. Menopausal women

Joints markets

1. Osteoarthritis2. Healthy Joint functions3. Osteoporosis

Applications

Part III. What is Osteoarthritis?Part III. What is Osteoarthritis?

Osteoarthritis (OA) is the most common form of age-related disease in people aged 65 and older.

It is a degenerative joint disease characterised by the breakdown of joint articular cartilage, triggering pain, stiffness

and swelling.

Rheumatoid arthritis (RA) is an autoimmune disease different from OA. In fact, the immune system attacks the body’s own

tissue as though it were a foreign invader.

People with RA produce rheumatoid factor, an immunity-related substance that targets the synovial membrane (protective layer that

encases joints). The consequences are severe pain and inflammation, joint pain and loss of joint movement & functions.

Part III. How does NatiCol work?Part III. How does NatiCol work?

Clinical studies have shown that hydrolysed collagen targets and has a positive effect on joint tissue 1, 2, 3,4, 7.

Daily supplements of 6-10 g of hydrolysed collagen are generally admitted to reduce pain and improve mobility when use with complementary ingredients.

It has been suggested that hydrolysed collagen stimulates chondrocyte production, which is involved in the biosynthesis of type II collagen, essential to new cartilage formation. 1, 2, 4, 6,

Part III. BibliographyPart III. Bibliography

• Oesser S Seifert J. (2003): Stimulation of type II collagen biosynthesis and secretion in bovine chondrocytes cutured with degraded collagen. Cell Tissue Res. 311 (3) : 393-9.

• Oesser S et al (1999) : Oral administration of 14C labeled gelatin hydrolysate leads to an accumulation of radioactivity in cartilage of mice (C57/BL). Nutrient matabolism 129, 1891-1895.

• Deal Cl, Moskowitz RW (1999): Nutraceuticals as therapetic agents in osteoarthritis. The role of glycosamine, chondroitin sulfate, and collagen hydrolysate. Rheum Dis Clin North Am. 25 (2) : 379-95

• Moskowitz RW (2000): Role of Collagen hydrolysate in bone and joint disease. Semin Arthritis Rheum.. 30 (2) : 87-99.

• Takeda et al 1982 (1982) : ACute and subacute toxicity studies on collageb wound dressing (CAS) in mice and rats. J Toxicol Sci. 7 (2): 63-91.

• Pesakova V, Stol M, Adam M. (1990) : Comparison of the influence of gelatine and collagen substrates on growth of chondrocytes. Folia Biol. 36 (5): 264-70.

• Novotna et al. (1991): The role of cartilage minor collagens in inducing arthritis. Z. Rheumato. 50 (2): 93-8.