single cell capture
DESCRIPTION
Capture of a single cell on a microfluidic chip.TRANSCRIPT
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Single bacterial spore detection Single bacterial spore detection with magnetoelastic particle sensors with magnetoelastic particle sensors
on a microfluidic platformon a microfluidic platformAuburn University Detection and Auburn University Detection and
Food Safety CenterFood Safety CenterJ.W. HongJ.W. Hong
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Chip Design – Top ViewChip Design – Top View
Unit: [um]Unit: [um]
Fluidic channels for sample loadingFluidic channels for sample loadingFluidic channels for sensor loading Fluidic channels for sensor loading Control channels Control channels
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Top View (Enlarged)Top View (Enlarged)
Unit: [um]Unit: [um]
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3D View3D View
Fluidic channels for sample loadingFluidic channels for sample loadingFluidic channels for sensor loading Fluidic channels for sensor loading Control channels Control channels
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3D View (Enlarged)3D View (Enlarged)
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Multilayer Soft LithographyMultilayer Soft Lithography
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Pneumatic Controlled ValvesPneumatic Controlled Valves
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Valve ActuationValve Actuation
Valve actuation pressure: < 3.5 psiValve actuation pressure: < 3.5 psi
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Fluidic channels for sample loadingFluidic channels for sample loadingFluidic channels for sensor loading Fluidic channels for sensor loading Control channels Control channels
Chip Operation with Food DyesChip Operation with Food Dyes
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Fluoresbrite YG 2.0-um microspheres (Polysciences, Inc.)Fluoresbrite YG 2.0-um microspheres (Polysciences, Inc.)
Chip Operation Chip Operation with Fluorescent Microsphereswith Fluorescent Microspheres
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Sensor LoadingSensor Loading
A particle sensor in the chamberA particle sensor in the chamber
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Spore CaptureSpore Capture
2um-fluorescent microspheres
Particle sensor