single-biomolecule circuits with carbon nanotube wiring brett goldsmith collins lab department of...
TRANSCRIPT
Single-biomolecule Circuits With
Carbon Nanotube Wiring
Brett GoldsmithCollins LabDepartment of Physics and Astronomy
John Coroneus and Greg WeissDepartment of Molecular Biology and Biochemistry
Overview: Creating a single-biomolecule circuit
Device Preparation
Electrically Measured Reactions
First: Redox Reactions
Second: Selective Reaction
Third: Proteins
PMMA/PEG
PMMA/PEG
Source
Drain
Nanotube Devices
R = 3MW
Electrochemical Oxidation of Nanotubes
ReferenceCounter
Source Drain
PMMAcoating
Electrolytedrop
Mannik et al. PRL (2006) Goldsmith el al. Science (2007)
Oxidation Dynamics
localization: session W26
Electrochemical Reduction of Nanotubes
Production of Carboxyl Groups by KMnO4
Goldsmith el al. Science (2007)
Selective Chemistry of Nanotubes
functionalized nanotube in:
buffer
buffer with 10 mM EDC
Protein Attached Circuits: Streptavidin
Goldsmith el al. Science (2007)
Protein Attached Circuits: Lysozyme
functionalized nanotube in:
buffer
buffer with enzyme substrate
Protein Attached Circuits: Lysozyme
Enzyme withoutsubstrate
Enzyme with substrate
360
420
Summary
Engineering the Microworld at The University of California, IrvineUCI Integrated Nanosystems Research Facility
Dr. Phil Collins
Brett GoldsmithAlex KaneBucky KhalapSteve HuntDanny WanACS-PRF
Electrically Measured ReactionsFirst: Redox ReactionsSecond: Selective ReactionThird: Proteins