SIM Scientific Instruments SIM Scientific Instruments

Manufacturer GmbHManufacturer GmbH

Im Erlengrund 21

D-46149 Oberhausen

Tel. +49(0)208-94 10 78-0

Fax +49(0)208-94 10 78-88

www.sim-gmbh.de

Scientific Instruments Manufacturer GmbH

Company History I

1997 formation of SIM GmbH in Muelheim,managing directors Rolf Eichelberg, Werner Kientopf

1998 shortly after start of production in the end of 1997, 2 new members of staff are recruited (1 precision mechanic and 1 precision mechanic apprentice)

1999 after a short interstation in Moers, SIM GmbH moves to Muelheim, Lutherstraße (200 m2 for workshop and offices), Muelheim, Lutherstraße (200 m2 for workshop and offices), transaction volumne 315 000 DM

2000 SIM GmbH becomes Channel Partner of Agilent Technologies

workshop in Muelheim during

move in, 1999

Company History II

2004 Pyrolysis System of Frontier Laboratories is incorporated in the product line (incl. service and maintenance)

2005 in January SIM takes over distribution, service, and maintenance for Markes thermal desorption systemstransaction volume 3.6 million Euro, 15 staff members

2006 move into the new company building in Oberhausen at the beginning of the year (750 m2 incl. laboratory, seminar and beginning of the year (750 m2 incl. laboratory, seminar and meeting rooms) transaction volume 4.8 million Euro, 15 staff members

SIM Company 2010

• Analytica 2010 in Munich: presentation of the new SIM

product line

• cooperation with foreign distributors

(SRA – France, Italy / JSB – Benelux, UK / LQA / USA)

• 24 staff members since August• 24 staff members since August

SIM Development Plan

end of the

1st phase of construction:

Dez. 2005

start of the

2nd phase of construction:

Nov./Dez. 2010

• development of functional chromatography

accessories

• custom-made chromatography solutions

Products & Services

• sales of chromatography, thermodesorption,

and pyrolysis systems, accessories, and

spare parts

• qualified consulting and support service

Beginning (1997)

manufacturing of special adapters for

gas chromatography

SIM S/SL Inlet (1999)

1. twist off the coupling ring

2. remount upper part of the inlet together with liner

3. change liner

4. mount upper inlet part with the new linernew liner

5. twist on the coupling ring

� gas tubing is not bent at each liner exchange

� no breakage of the tubing

� convenient maintenance

MSD Direct Inlet Probe (2002)

Analytica 2002:

DIP for 5973MSD new appearance

adaptation to 5975 MSD

and new colour(according to changeof 6890GC)

DIP for Agilent 5975 MSD + 7000 Triple Q (2010)

Hydrogen Sensor

integrated in

7890 GC

integrated in

6890/6850 GC

external version formany other GCs

Hydrogen Sensor

� eliminates any risk of explosion

� gas sensor mounted in the oven:

constant detection of hydrogen in the

GC oven

� LEDs show the measured gas

concentrationconcentration

� H2 -concentration above preset

threshold (10 % LEL*): acoustic and

optical signals dependant on H2-

concentration

� hydrogen alarm (25 % LEL – 1% H2):

safety valve unit switches to inert gas

*LEL – lower explosion limit

Hydrogen Sensor - Specifications

� integrated devicegas sensor, carrier gas switch and LED display together with power supply integrated in GC 7890 / 6890

� external device (for Agilent and most other GCs)gas sensor integrated in GC oven , carrier gas switch and LED display are in external controller

≙

� detection range0 – 25 % LEL* (≙ 0 – 1.0 % by vol. H2)

� 0.4 % by vol. H2

optical alarm: first red LEDacoustic alarm: starts with intermettent beep

� 1.0 % by vol. H2

optical alarm: 4 red LEDs, acoustic alarm continues, carrier gas is switched to N2 / He

*LEL – lower explosion limit

OFD Olfactory Detector (Sniffer)

� absolutely olfactory neutrality of all components,

esp. the insulating of the transfer line (odorless

even at temperatures up to 300 °C)

� heatable transfer line without “cold spots“,

optional with temperature program corresponding optional with temperature program corresponding

to the GC run

� addition of humidified air prevents the nasal

mucosa from drying out

� comfortable working position due to the vertically

adjustable sniffing port

OFD Olfactory Detector (Sniffer)

� simultaneous detection with other GC detectors

(e.g. FID, ECD, MSD) using different splitters

(standard parallel splitter, microfluidic splitter,

microfluidic Deans Switch) with manual control or

EPC

� different ways for rapid recording of odor � different ways for rapid recording of odor

impressions and intensities (short cuts, menu bar,

via keyboard or pen tablet)

� integrated in Agilent ChemStation / MS ChemStation:

• odor impressions/intensity added to chromatogram

• temperature of transfer line and all gas flows are

saved in the methods•olfactogram is saved in the folder of the GC run

1-Octen-3-on(character impact compound)

Intensity: +6

Odor Impression:

fresh mushrooms

(pilzig, frische Champignons)

Retention Time:

23.41 min

OFD Analysis of a Mushroom Extract

1-Octen-3-one

23.41 min

GC: Agilent 68/7890, column Varian CP7889 50m x 0.32mm x:0.4µm, Inlet: PTV, Split 1:1, 250 °C, carrier

gas: He (1.27 ml/min), oven program: 50 °C for 5 min, to 240 °C at 2 °C/min, hold for 60 min

MSD: capillary to MSD: 0.4 m x 0.1 mm x 0 µm, He: 2.36 ml/min, EI-Scan 35-390 amu

OFD: capillary to OFD: 1.28 m x 0.1 mm x 0 µm, He: 5.0 ml/min,

Octanal

Sample:

Chinese Matsutake

(Tricholoma matsutake),

pentane/dichloromethane

extract(detail 21.5 – 27 min)

ICECUBE – Multi Cooling Device

ICECUBE: PTV Inlet Assembly

ICECUBE: PTV-Temperature Profile

PTV cooling with ICE-CUBE (blue line) and without ICE-CUBE (red line):

7890 GC with oven constant 60 °C,

PTVinitial: 350 °C 7890 GC with oven: 325 °C (for 15 min), fast

cool down mode to 30 °C, PTVinitial: 350 °C

���� cooling down the PTV with ICECUBE to 30 °C needs only 6.5 min , simultaneously with an oven temperature of 30 °C, the PTV reaches 0 °C (14 min)

���� at a constant temperature of 60 °C, the PTV with ICECUBE can be cooled down to 30 °C within only 5 minutes

ICECUBE – Multi Cooling Device

� compressor cooling for all

temperature controlled GC inlets,

Agilent G4514A autosampler tray,

and SIM CryoTrap

� PTV cooling down to 0 °C (at oven � PTV cooling down to 0 °C (at oven

temperature < 55 °C)

� fast and convenient without the coolants LN2, LCO2

� energy-saving due to discontinuous cooling

� maintenance free

ICECUBE: Specifications

GC Inlets (PTV,MMI) Agilent Tray G4514A

ICECUBE No. B 0800 04 50 B 0801 04 50

Tmin (ICECUBE cooling) 0 °C(GC oven < 55 °C)

5 °C

Tmin (LCO2 cooling): - 60 °C -

Tmin (LN2 cooling,optional): - 160 °C -

Controls integrated in GC 6890/7890 (using 24 V DC solenoid valve)

integrated in ICECUBE itself

B 0800 04 50 / B 0801 04 50:

size H: 25 cm (9.9 in.), W: 23 cm (9.1 in.), D: 53 cm (20.9 in.) weight 21 kg (46.3 lb)

electric parameters 230/120 V, 50/60 Hz, 1.1 / 2.2 A, 250 Wmax. allowableworking pressure 25 barambient temp. limit + 5°C to + 32°Crelative humidity max. 85 %

Noise Reduction Enclosures

� minimizing noise in the workplace

noise absorption approx. 9 dB(A)

� integrated safety items avoid overheating

- integrated fan as protection against

excessive heat- when pump is turned on the fan is working

Noise Reduction Enclosures

- when pump is turned on the fan is workingautomatically

- cutout switch > 60 °C

� lightweight construction, dividable into two

parts for easy handling

� available for pumps, cryostats and other

divices with high noise emission

� standard sizes as well as sizes on requestpreliminary model

� to illuminate the GC oven

during maintenance work

Oven Lamp

during maintenance work

� easy change of GC columns

lamp is switched on when the door is opened

HT-HPLC 200 column oven

for Temperature Programmed Separations up to 200°C

�fast LC without organic modifier for all HPLC systems

� subcritical water as mobile phase: low dielectric

constant so that no or only small amount of

organic modifier is needed

� decreasing viscosity with increasing

temperature:

� higher flow rates, fast separations

� temperature gradients instead of solvent

HT-HPLC: Principle

� temperature gradients instead of solvent

gradients

� optimal separations by the use of combined

temperature and solvent gradients

� applications for sulfonamides, pesticides ,

steroids, food preservatives etc.

� particularly suitable for IRMS (isotope ratio

mass spectrometry) where organic eluents

cannot be used

� max. 200 °C, 30 °C/min,

temperature program with 3 ramps

� 3 temperature zones: eluent preheating (1),

column heating (2), cooling in front of detector (3)

� effective cooling (compressor) , for temperature

sensitive detectors, short analysis cycles

HT-HPLC: HT HPLC 200 column oven

3

2

sensitive detectors, short analysis cycles

� principle: contact heating (column and capillary

embedded in aluminium blocks) – for fast and

precise temperature control

� exact temperature control

� temperature gradients

� high reproducibility

13

HPLC-Säule

SIM

AL blockcolumn

heating element

HT-HPLC Application: Steroids

� fast isothermal separation of a steroid mixture (1.4 min):Lit.: Teutenberg, T. et. al., J. Chromatogr. A, 114 (2006), 89-96

0 - acetonitrile

1 – estriol

LC System: Agilent 1200 SL and HT-HPLC 200 column ovencolumn: Zirchrom-PBD; 150x4.6 mm; 3 µm, 300 A mob. Phase: water/acetonitrile , ratio and temperature see fig.detection: UV (200 nm)

1 – estriol

2 – androstadienedione

3 – dehydroepiandrosterone

4 – estrone

HT-HPLC Application: Pesticides

� Separation of 250 pesticides in 20 min using temperature and solvent gradients :

LC system: Agilent 1200 SL and HT-HPLC 200 column ovencolumn: Zorbax C18 SB; 50x3mm; 1.8µm mob. Phase: gradient H2O/acetonitrile, 1.4 mL/min, temp. gradient 70 °C to 90 °Cdetection: TOFMS

HT-HPLC Application: Sulfonamides

� Separation of 5 sulfonamides and uracil with the eluent water (baseline separation for IRMS)Lit.: Jochmann, M. et al., GIT Labor-Fachzeitschrift 3 (2010), 182-185

1 – uracil

2 – sulfadiazin

column: Waters Xbridge C18; 75x4.6 mm; 2.5 µm mob. Phase: H2O, 0.1 % formic acid, 1 mL/min, temp. gradient 0 °C to 180 °Cdetection: UV (270 nm)

2 – sulfadiazin

3 – sulfathiazol

4 – sulfamerazin

5 – sulfamethoxazol

6 - sulfamethazin

Thermostatted Fluorescence Detector

� thermostatted flow cell integrated in

Agilent fluorescence detector

� high reproducibility of the fluorescence

measurement as fluorescence is

temperature sensitive

� temperature regulation with peltier

element

� additional conditioning of the column

eluate for constant detection temperature

� measurement independant of ambient

temperature