shiri freilich janet thornton’s group, ebi cambridge university
DESCRIPTION
Relating the evolution of gene content to tissue specialization. Shiri Freilich Janet Thornton’s group, EBI Cambridge University. “...And when he was 93, Grandpa decided that the time had come for a man-to-man conversation. (I was 36 at the time, I have been married for 15 years). - PowerPoint PPT PresentationTRANSCRIPT
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Shiri FreilichJanet Thornton’s group, EBICambridge University
Relating the evolution of gene content to tissue specialization
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“...And when he was 93, Grandpa decided that the time had come for a man-to-man conversation.
(I was 36 at the time, I have been married for 15 years).
‘All my life I am all the time looking at woman, looking and learning. Nu, and what I learned, I want to learn to you now also.’
‘Woman, in some ways is just like us exactly the same. But in some other ways a woman is entirely different.’
‘But you know what? In which ways a woman is just like us and in which ways she is very different –
nu, on this I am still working’.”
(Amos Oz, A Tale of Love and Darkness)
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The full genome sequence from various species can highlight the common versus unique
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What’s new?
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The transition from unicellularity to multicellularity as an interior
design challenge
- gene
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Overview
• Part 1: Expression pattern of ‘old’ and ‘new’ proteins in mouse tissues
• Part 2: Expression pattern of singleton and duplicate proteins in mouse tissues
• Part 3: The evolution of tissue-specific metabolic pathways in mammals
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Part 1:
Relating age and function of a protein to its expression pattern in mouse tissues
A collaboration with Tom Freeman’s group
(MRC RFCGR)Freilich et al, Genome Biol. 2005;6(7):R56.
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Processing mouse expression and sequence data
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Classification of mouse proteins into phylogenetic groups
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Tissues have a similar composition of functional classes
Tissue type
Frac
tion
enzymes
transcription regul.signal transductiontransporters
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Tissues have a similar composition of phylogenetic classes
Tissue type
Frac
tion
universaleukaryote sp.metazoan sp.mammalian sp.
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Expression distribution of proteins from different
categories• Tissues have almost identical functional/
phylogenetic composition• Tissue diversity must be achieved through
differences in the protein composition within each category
• Do proteins from different categories duffer in their expression pattern?
Number of tissues where protein is expressed
Frac
tion
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Regulatory proteins are more specifically expressed
Number of tissues where is expressed
Frac
tion
~1/3
~1/10
Mouse proteinsclassified into functional groupsRegulatory
proteins
Metabolic proteins
enzymes
transcription regul.signal transductiontransporters
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Metazoan-specific proteins are more specifically expressed
Present in a unicellular ancestor of metazoa
Number of tissues where protein is expressed
Frac
tion
universaleukaryote sp.metazoan sp.mammalian sp.
Mouse proteinsclassified into phyletic groupsSpecific to
metzoa
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Functional categories overlap with phylogenetic categories
• Most of the pre-metazoan proteins are metabolic proteins (transporters and enzymes)
• Most of the metazoan-specific proteins are regulatory proteins (signal transduction and transcription regulation)
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Identifying the dominant influence: function or age
• Obvious differences between ‘old’ and ‘new’ proteins, within the metabolic functional group
• Yet, less than 1/3 of the pre-metazoa proteins are expressed in all tissues
Number of tissues where protein is expressed
Frac
tion
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Still, some of the pre-metazoa proteins are tissue specific
• Functions occurring in the unicellular cell become tissue-specific in multicellular species (Ldh example)
• Universal genes that have been duplicated become specific to a tissue whilst a second copy maintains its original expression pattern (Pgk-2 example)
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Part 2:
Relating duplication events to expression pattern in mouse tissuesFreilich et al, Genome Biol. 2006;7.
“…duplication events had contributed greatly to the attainment of the complex body organisation in metazoa, where cells having identical genetic material can differentiate … due to the presence of duplicated genes in their genomes” Ohno S. (1970). Evolution by gene duplication.
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The subfunctionalization model
Lynch M & Force A, Genetics. 154 (2000):The division of expression of an ancestor gene between its daughter duplicates promotes the retention of a gene in the genome
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Microarray expression data provide support to the
subfunctionalization model• Gu et al: expression divergence between
duplicate genes increases with evolutionary time (differentiation modes in yeast). Trends Genet. 2002;18: 609-13.
• Makova et al: spatial expression divergence between duplicate genes increases with evolutionary time (human tissues). Genome Res. 2003;13:1638-45 .
• Huminiecki and Wolfe: a general trend for increased tissue-specificity of expression as family size increase was observed for mammalian genes. Genome Res. 2004;14:1870-79 .
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Project Overview:The relationship between gene
duplication and breadth of expression
Protein’s perspective: 1. Does duplication event lead to an increase in tissue
specificity?
Time of duplication perspective:2. Does the date of duplication event matters? (i.e., do
duplication events occurring in the ancestral unicellular lead to an increase in tissue specificity)
Protein-family perspective: 3. does a protein family maintain a non-specific expression
pattern? (i.e., is a specific expression of proteins from big families complementary)
(Freilich et al, Genome Biol. 2006;7(10):R89)
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Singleton proteins are more globally expressed
Number of tissues in which protein is expressed
Frac
tion
Singletons (570) Duplicate proteins (1886) Proteins with many close homologues (417)
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Negative correlation between expression breadth and number
of homologues
Groups of proteins, ordered by their number of homologues
Mea
n nu
mbe
r of
ti
ssue
s
SingletonsDuplicate proteins
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Large variation
Number of homologues proteins
Num
ber
of t
issu
es
Correlation -0.20P-value 1.5e-55
SingletonsDuplicate proteins
Mean number of expressed tissues
Correlation -0.20 P-value 1.5e-55
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Does the date of duplication event matter?
(i.e., do duplication events occurring in the ancestral unicellular lead to an increase in tissue specificity)
?
Increase in tissue specificity
Global expression
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Identifying ‘old’ and ‘new’ duplications
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Only post-multicelullarity duplication events lead to
expression specificity
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The protein-family perspective: Is the specific expression of
family members complementary?
Proteins from big families tend to be more specifically expressed.
Does a protein family maintain a non specific expression pattern?
?
Complementary expression
Overlapping expression
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Calculating the cumulative tissue distribution of protein
families
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Cumulative tissue distribution of protein families is not
correlated with family size
Complementary expression pattern in protein families:
While a duplication event leads to a tissue specialisation of one or
both copy, the total tissue-distribution of the protein family remains
constant.
Protein families, ordered by size
Aver
age
tiss
ue-
cove
rage
of
prot
ein
fam
ilies
Singletons
Families with any expression information
Families with >=75%expression information
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The findings support the subfunctionalization model
Protein’s perspective: 1. Does duplication event lead to an increase in
tissue specificity? YesTime of duplication perspective:2. Does the date of duplication event maters?
only duplication events that that place in a multicellular species lead to a specific expression-> suggests that expression divergence, following gene duplication, promotes the retention of a gene in the genome
Protein-family perspective: 3. Does a protein family maintain a non-specific
expression pattern? Yes-> suggests the division of expression between family members
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Part 3:The evolution of the mammalian
metabolic pathways
Some of the tissue-specific pathways are specific to mammals.
Can we understand how tissue-differentiation of animals’ metabolism reflects their evolution?
Freilich et al, BMC evolutionary biology 2008, 8:247.
STEROID HORMONE METABOLISM
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Why studying metabolic networks?
• Metabolic networks’ structure and composition are well defined
• Available metabolic databases
• Genotype is highly related to phenotype
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The structure of the KEGG database
STEROID HORMONE METABOLISM
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The reactions within a pathway can be absent/present in a
species
Arabidopsis Thaliana
Homo Sapiens
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Project overview:
• Identification of pathways absent/present in a species
• Classification of human pathways according to their phyletic origin
• Characterization of lineage-specific metabolic pathways
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Phylogenetic classification of human pathways
All pathways in human (metabolic)Universal pathways
Eukaryota-specific pathways
Metazoan-specificpathways
Mammalian-specific pathways
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Components of theEukaryotic membrane(sphingolipids, glycosaminoglycan)
Phylogenetic classification of human pathways
33 Universal pathways
8 Eukaryota-specific pathways
10 Metazoan-specific pathways
14 Mammalian-spc. pathways
Metabolic skeleton: sugars, nucleotides, some amino-acids, energy
Tissue specific activities:Neuronal guidance, hormonal activity, digestion Tissue specific activities and intracellular signaling(blood cell recognition)
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The pathways can be linked to form a network
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The network structure of the metabolic pathways
Universal pathwaysEukaryota-spc. pathwaysMetazoan-spc. pathwaysMammalian-spc. pathways
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The integration of the steroid biosynthesis pathway into the sterol biosynthesis pathway
sterol
cholesterol
steroid hormonebile acid
UniversalEukaryotaHuman
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From manually selected examples to a computational approach
Creating a list of adjacent reactions:
2.7.4.2 -> 4.1.1.334.1.1.33->2.5.1.1..2.5.1.1->2.5.1.212.5.1.21-
>1.14.99.7
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Using the adjacency list for a large-scale characterization
of the metabolic network
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What’s new (metabolic pathways)?
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Summary
• ‘New’ genes tend to be more tissue-specific, ‘ancient’ genes tend to be globally expressed
• Despite this trend, many metazoan genes are ubiquitous and many universal proteins are tissue specific
• ‘New’ duplications of ‘old’ and ‘new’ proteins lead to a more specific expression, and therefore can facilitate the evolution of new, tissue-specific, functions
• The core of metabolic-pathways, inherited from a uniclellular ancestor, provides a platform for the evolution of mammalian-specific, tissue-specific pathways
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Thanks
Thornton GroupJanet ThorntonTim MassinghamEric BlancExpression data:Tom Freeman
Sumit Bhattacharyya