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Food & Nutrition Shelf-life of Australian vacuum packed beef-impact of meat colour Joanne Hughes| CSIRO Coopers Plains Mintrac Meat Inspection & Quality Assurance Conference 21 st Oct 2015 You can change this image to be appropriate for your topic by inserting an image in this space or use the alternate title slide with lines. Note: only one image should be used and do not overlap the title text. Enter your Business Unit or Flagship name in the ribbon above the url. Add collaborator logos in the white space below the ribbon. [delete instructions before use]

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Food & Nutrition

Shelf-life of Australian vacuum packed beef-impact of meat colourJoanne Hughes| CSIRO Coopers PlainsMintrac Meat Inspection & Quality Assurance Conference 21st Oct 2015

You can change this image to be appropriate for your topic by inserting an image in this space or use the alternate title slide with lines.Note: only one image should be used and do not overlap the title text.

Enter your Business Unit or Flagship name in the ribbon above the url.

Add collaborator logos in the white space below the ribbon.

[delete instructions before use]

Extension of shelf-life to promote global competitiveness

Falling $AUS, advantageous to export markets

Opportunities for growth in other export markets-Free Trade Agreements (FTAs)Increased demand for high quality Australian beef- especially with the rising Asian middle class

Enhancing our global competitiveness-differentiation in global environment

Background research

30 weeks (-0.5°C ) Micro & sensory

ok

pH can impact on micro &

sensory

Consumer acceptance

lower in darker or paler

muscles

Does meat colour impact shelf-life & eating quality?

“bitter”“sour”“off”

“less flavoursome”“less tender”

Long aged (20 weeks) vacuum packed chilled storage- design3 meat colour scores

1B, 1C (light)2, 3 (medium)4 and above (dark)

324 striploin samples from 3 export plants (mix of pasture & grain fed animals)

Stored at -1°C for 20 weeks at CSIRO

Assessed at 0, 2, 8, 12, 16 and 20 weeks

Determine effect of beef meat colour grade on:Biochemical propertiesMicrobiological growthEating qualityRetail display

Storing for longer reduced appearance & odour scores

Darker colours less acceptable after week 16

Appearance & odour on opening

3

12.00

4

20.00

5

6

2.00

7

8

0

2

8.00

1

16.00

App

eara

nce

scor

e

storage week 12.00 8.00 2.00

8

6

4

2

20.00 0

16.00

7

5

1

3

storage week

Odo

ur s

core

LightMediumDark

Acceptable

Unacceptable

Biochemical properties

Dark meat had a higher pH & less glycogen whereas light & medium colours had a lower pH- similar values observed

0

5.8

5.4

20 5.0

16 12 8

6.0

2

5.6

5.2

storage week

pH

LightMediumDark light medium dark

Glycogen (µmol/g) 37 37 33Lactate (µmol/g) 22 22 20

Dark colours had a higher pH

MLA 2011

Total viable count (TVC)-Increased at week 8 and reached a plateau- After week 8, dark muscles had higher values compared to light & medium coloured muscles

Microbiology- TVC increased at 8 weeks and was higher in dark meat

Dark colours higher TVC

12 8 2 0

8

6

4

2

20 0

16

5

7

3

1

storage week

Tot

al v

iabl

e co

unt (

TVC)

LightMediumDark

TVCs & LABs:- No major differences observed between plants- 2 plants with hot water

decontamination units

Brocothrix:- Similar increase at week 8,

but no difference between meat colours

Microbiology- LAB showed similar to TVCDark colours higher lactic acid bacteria

(LAB)

8 2 0

6

4

2

20 0

16 12

5

7

3

1

storage week

Lac

tic a

cid b

acte

ria (l

og cf

u/sq

cm) Light

MediumDark

MQ4 score (out of 100) was calculated by adding 4 sensory components:• Tenderness 30%• Overall liking 30%• Flavour 30%• Juiciness 10%

• MSA eating quality guide separated into:– 3, 4, 5 star ratings

Eating quality using Meat Standards Australia (MSA)- weeks 2, 12 & 20 only

Improved eating quality with storage > 12 weeksLighter colours continue to improve

MSA MQ4 by colourLight colours continue to improve

3

4

40

12

45

50

2 20

55

60

MQ

4 sc

ore

Storage week

LightMediumDark

Tenderness and juiciness scores increased with storageFlavour started to decrease at 20 weeks, apart from in the light coloured group

Tenderness, juiciness & flavourLight colour continues to improve

1

Lighter colours continued to improve

in eating quality

35

40

45

12

50

55

60

65

2 20 Storage week

Ten

dern

ess

LightMediumDark

35

40

12

45

50

55

20 2

60

Juici

ness

Storage week

LightMediumDark

40

12

45

50

2 20

55

60

Fla

vour

Storage week

LightMediumDark

Colour groups had a similar retail display life

Retail display steaksWeek 20, after 3 days retail display

Light

Medium

Dark

Steaks visually suitable 2 weeks = 6 days

12 weeks = 4 days20 weeks = 3 days

In summaryStoring meat reduced the appearance and odour of the product, but all meat

colours were still acceptable at week 20.

The development of spoilage associated microflora was more pronounced in dark muscles & consequently these muscles have a higher likelihood of reduced storage life.

All meat colours improved in eating quality with aging, especially between 2 and 12 weeks.

After 20 weeks storage, MQ4 scores indicate a 3 star product is achieved, meaning light & medium meat colours can achieve a “good everyday” eating quality when stored appropriately.

Future studies/ going forward

Determine shelf life/eating quality of long aged beef for export markets- “real life situation”

- especially to emerging markets- Biochemical, microbiological & sensory/ eating quality

Current project looking at the effect of purge on shelf-life of vacuum packed chilled meat, especially for Egyptian clients.ABARES Beef & veal Pork Lamb Chicken

Export vol 2013-14kt%

1,184/1611

74%

47/360

13%

250/ 470

53%

38/1066

4%

Main export markets

USA, Japan & China

USA, Canada& China

Middle east,USA & China

AcknowledgementsFunding from:

• Meat Livestock Australia

• Australian Meat Processors Corporation

Sensory:

• Rod Polkinghorne & Alan Gee

• CSIRO team & statistican (Gavin Kearney )

• Beef Processors

Thank youCSIRO Food & NutritionJoanne Hughes

t +61 7 3214 2068e [email protected] www.csiro.au