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StarGenetics Exercise Development Excel Workbook: Fly Visualizer

Introduction1. StarGenetics exercises are developed in Excel. You can modify this Excel Workbook to develop your own StarGenetics exercise.

2. This Excel Workbook contains 4 additional tabs:

-Mating Engine: this tab specifies the type of visualizer used within the software and its specific characteristics.

-Genes & Alleles: this tab specifies the alleles and genes in question and their location within the genome.

-Genes interaction: this tab defines the relationship between genotypes and phenotypes.

-Organisms for mating: this tab defines the organisms presented by StarGenetics to the users.

The information presented in each tab is divided into 'Instructions' and 'Input'. 'Instructions' describes how to specify 'Input', which is the information

within the Excel workbook that the software utilizes.

3. StarGenetics can open any properly formatted exercise that has been developed in Excel like this one (for ex: StarGenetics will open this exact Excel file as is).

However, encrypting them is recommended so that users do not have access to the information. You can then provide the encrypted version to your users for the

To convert an Excel file into an encrypted SGZ format, click on 'Tools -> SGZ encryptor' and follow the instructions.

For further information on StarGenetics go to: http://web.mit.edu/star/genetics/



M ti i thi t b ifi th t f i li d ithi th ft d it ifi h t i ti



Mating engine: this tab specifies the type of visualizer used within the software and its specific characteristics

INSTRUCTIONS-Name: name of organism.

-Visualizer: currently the following visualizers are supported:

Fly

Yeast

Fish

Smiley Face

Peas

XX/XY

MATa/MATalpha

Self

-Total progeny per cross

-Male/Female recombination rate:

a recombination rate of "0" means there is no recombination (for ex: recombination in Drosophila males does not occur)

a recombination rate of "1" means that recombination frequency is equivalent to the actual distance between genes

-Matings: number of matings that an individual fly can participate in.

INPUTName Pisum sativum

Visualizer Peas

Mating type Self

Total progeny per cross 8

Male recombination rate 1

Female recombination rate 1

Matings 100

-Mating type: the mating types currently supported at this moment are:

& A t t t t t t t t



enes & A e es: t s ta spec es t e a e es an genes n quest on an t e r ocat on w t n t e genome

INSTRUCTIONS

NOTE: The information contained in 'Input' within this tab is never presented to the student.

-Alleles: for each gene in question, name the alleles, separated by a comma. Order of alleles does not matter. You can use upper and lower case letters (E.g., W

-Gene: specify the gene name for each set of alleles.

-Chromosome: specify the chromosome where the desired genes are found.

-Gene Location: relative location of genes within a chromosome is defined in centimorgans and determines the recombination rate between genes.

-Notes: the information in 'Notes' is not presented to the software, but can be used to assist with problem development.

INPUTAlleles Gene Chromosome Gene Location Notes

S,s Plant height 1 1 Tall dominant

W,w Flower color 2 1 Purple dominant

T,t Flower Pod position 3 10 Axial dominantY,y Pod color 4 1 Green dominant

P,p Pod shape 5 1 Inflated dominant

U,u Pea color 6 1 Green dominant

R,r Pea shape 7 1 Smooth dominant



Genes interaction: this tab defines the relationship between genotypes and phenotypes



Genes interaction: this tab defines the relationship between genotypes and phenotypes

INSTRUCTIONSNOTE: The information contained in 'Input' is never presented to the user.

1. Specify the genotypes that result in NON WILD TYPE phenotypes for ANY of the phenotypes in question.

If a genotype is not specified, then the software will assign the DEFAULT WILD TYPE phenotype for the genotype in questi

- Space ( ) to indicate different sets of alleles found in the same gene (for ex: "y1 y2, Y1 Y2")

For alleles associated with dominant phenotypes: you can abbreviate the number of genotypes that would need to be

(for ex: the "Y2" allele is associated with a dominant body color phenotype --> you can substitute "Y2,y2" and "Y2,Y2", wit

3. Indicate the mutant phenotype for each genotype specified. The current phenotypes supported for the peas visualiz

-Plant height: defines the height of the plant stem (tall or short).

-Flower color: defines the color of the flower petals (purple or white).

-Flower & pod position: defines the location(s) of flower and pod development on the plant (axial or terminal).

-Pod color: defines the color of the pods on the plant (green or yellow).

-Pod shape: defines the shape of the pods on the plant (inflated or pinched).

-Pea color: defines the color of the peas that develop on the plant (green or yellow).

P h d fi th h f th th t d l th l t ( th i kl d)

2. Use the following nomenclature to describe genotypes:

- Comma (,) to indicate alleles of the same gene (for ex: "W,w" or "w,w")

- Semicolon (;) to indicate alleles from different genes (for ex: "w,w;b,b")



Pea shape

SMOOTH

Organisms for mating: this tab defines the organisms presented by StarGenetics to the users



Organisms for mating: this tab defines the organisms presented by StarGenetics to the users

INSTRUCTIONSNOTE: Information within the 'Traits' and 'Determinants' columns is not read by the software and is used to assist with problem development.

1. Create a column for each of the organisms that you would like StarGenetics to present to the users.

2. Assign a name for each of the organisms provided to the users and place that name as a column header. This name will be presented to the users.

3. Describe the following traits for each of the organisms provided to the users: - Genotype for each gene/alleles in question: annotate the genotype for each of the organisms. Use a separate cell for each allele/gene and comma to sepa

Order of the alleles does not matter.

INPUTTrait Determinants Purple flowered plant 1 Purple flowered plant 2 White flowered plant Mystery mutant

Plant height S,s S,S S,S S,S S,S

Flower color W,w W,W W,w w,w W,W

Flower Pod position T,t T,T T,T T,T T,T

Pod color Y,y Y,Y Y,Y Y,Y y,y

Pod shape P,p P,P P,P P,P p,p

Pea color U,u U,U U,U U,U U,U

Pea shape R,r R,R R,R R,R R,R

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