585 - Long term decomposition studies of wooden testblocks at Khao Yin National Park, Thailand

S. Sivichar, N. Boonyeun, N.L. Hvwel-Jones E.B.G.Jones

RIOTEC-Alycology, National Center fin- GeneticEngineering and Biotechnology, National Science andTechnology Development Agency, 113 Pahonyothin Rd..Khlong 1, Khlong Luang, Pathum Thanil 2120, Thailand.

In a survey of wood decay, 4 woods (Dipterocarpus alatus,ilia dolabriformis, Alstonia sclzolaris and Anisopteraoblonga) were immersed in two streams: Tad Tha Phuflows year-round; kni29 is ephemeral. These wererecovered each month over 1 year. 236 spp. of fungi werefound. Importantly, after 1 year decomposition had barelybegun. Usin g the same sites the work was extended toexamine other woods and to allow for recovery of up to 10yr. Work after 1 yr allows direct comparison with thepreliminary study. 5 spp. were used: Buchanania latijblicz,A .lanziteTa caloneura, Sindora siaznensis, Stereospermumneurctnthum and Tectona grandis. Buchanania and Tectonasupported the hi ghest diversity (20+ spp.) while Mangifferaand Sindora had <20 spp. each after l year. Diversity washigher- at lan29 (i6- 7 8 r;pp.) than at Tad Tha Phu (11-2'2spp.). This pattern held for each wood except Sindorawhere 16 spp. were found at km29 and 19 at Tad Tha Phu.At km29 Massarina thalassioides and Nectria sp01 werefound on all 5 wood while at Tad Tha Phu Trichoderma.sp., Candelabrum brocchiatom and Cancellidiumapplununim were on all 5 woods. After 1 year, diversity offun gi colonisin g was generally low. 30 spp. were foundfrom the 5 woods at km29. However, 4 of the 5 woods had<10 spp. The exception was Tectona with 20 spp.: 11 beingexclusive to Tectona. At Tad Tha Phu 21 spp. wererecorded at 12 months: Mangifera had 4 spp. while themaximum of 10 was from Shuford 6 of these wereexclusive to Sindora.

586 - Detecting diversity of ectomycorrhizal fungi inDouglas-fir dominated forests

J.E. Smith, T.A. Dreisbach' * , M. Brown' & R. Molina'

, USDA-Forest Service, PNW Research Station, 3200Jefferson Way, Corvallis, OR 97331, - 2S:vnthesis

Research & Analysis, 5326 SE Hawthorne Blvd., Portland,OR 97215, U.S.A.

Surveys for ectomycorrhizal mushroom and trufflesporocarps over 4 fall and 3 sprin g seasons resulted in alarge data set suitable for an analysis of the effect ofsampling intensity on observed fun gal diversity andabundance. An impressive 48 truffle and 215 mushroomtaxa were collected from three replicate stands in each ofthree forest age classes (young, rotation-age, and old-growth) of Douglas-fir dominated stands in the CascadeRange of Ore gon. Cumulative species richness was similaramong forest age-classes but differed between seasons.

Seventeen species accounted for 79% of the total sporocarpbiomass. We investigate and present l) how the observeddiversity and abundance relates to sampling intensity, 2)the likely number of species extant in the study area butundetected by the survey, 3) the sampling intensitynecessary to detect each known species. and 4) how thestatistical power to detect a si gnificant difference in fun,/aldiversity or abundance varies with samplin g intensity.Knowledge about methods for detecting the diversity ofcommon and uncommon ectomycorrhizal fun g al species isessential to maintaining the biological diversity of fungi.

587 - Effect of ectomycorrhizal inoculation on thegrowth of Cyclobalanopsis edithiae seedlings

K.Y. So' W.K. Chan

Hong Kong Polytechnic University, Hung H0171, Kowloon,

Hong Kong, China. - E-mail • ken01@lletvigator.com

Field collected soils and roots under a community of anative Fagaceae, Cyclobalanopsis edithiae, were used as aninstant inoculum in this study. Acorns of C. edithiae ofuniform wei ght and size were surface sterilized and sownon sterile sand for germination. The germinated seedlingswere planted individually into a I L plastic pot containing200 ml of field soil inoculum. An uninoculated controltreatment was also set up. The pots were arranged incompletely randomized block design and were placed in atemperature controlled unit at 25 °C. 5 seedlings wereharvested from both the control and the inoculated pots forgrowth analysis every 8 weeks. Root collar diameter, shootand root thy weight, leaf area, and the percentage ofectomycoiThizal colonization were measured andevaluated. The avera ge colonization rates of the inoculatedseedlings in the three harvests were 8.48. 14.28%, and10.40%. No infection was observed in the controlseedlin gs. The relative growth rate, root dry weight andtotal dry weight were significantly (p<0.05) hi gher in theinoculated seedlin gs than the control in all the threeharvests. The result su ggested that such low-cost andconvenient inoculation method could be further exploredfor the production of Fagaceae seedlings in nurserycondition.

588 - Fungi from North-West Caucasus: from themountains to the molecular level

M.V. Sogonov

Rdeyskv State Natural Reserve, Chelpanova Str. 27,175270 Ehohn, Novgorod Region, Russia. -sylvatica@fronzrzi.com

Soil microfungal communities were studied in 8 high-altitude ecosystems in Tebercla Reserve (Karach aevo-

Cherkessia, Russia): Rhododendron Lulu:talcum bushes,alpine fens, alpine snow beds, Geranium gytmrocazilon -

Heclvsarzon caucasicurn meadow, Festuca varia grassland'

178 Rook of ..kbstracts