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separation mechanism

18
1 Separations Separation in based upon differential migration between the stationary and mobile phases. Stationary Phase - the phase which remains fixed in the column, e.g. C18, Silica Mobile Phase - carries the sample through the stationary phase as it moves through the column. Injector Detector Column Solvents Mixer Pumps High Performance Liquid Chromatograph Waste

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Page 1: Separation mechanism

1

Separations

Separation in based upon differential

migration between the stationary and

mobile phases.

Stationary Phase - the phase which

remains fixed in the column, e.g. C18,

Silica

Mobile Phase - carries the sample

through the stationary phase as it

moves through the column.

Injector

Detector

Column

Solvents

Mixer

Pumps

High Performance Liquid Chromatograph

Waste

Page 2: Separation mechanism

2

Separations

Injector

Detector

Column

Solvents

Mixer

Pumps

Chromatogram

Start Injection

mAU

time

High Performance Liquid Chromatograph

Page 3: Separation mechanism

3

Separations

Injector

Detector

Column

Solvents

Mixer

Pumps

Chromatogram

Start Injection

mAU

time

Page 4: Separation mechanism

4

Separations

Injector

Detector

Column

Solvents

Pumps

Mixer

Chromatogram

Start Injection

mAU

time

Page 5: Separation mechanism

5

Separations

Injector

Detector

Column

Solvents

Pumps

Mixer

Chromatogram

Start Injection

mAU

time

Page 6: Separation mechanism

6

Separations

Injector

Detector

Column

Solvents

Pumps

Mixer

Chromatogram

Start Injection

mAU

time

Page 7: Separation mechanism

7

Separations

Injector

Detector

Column

Solvents

Pumps

Mixer

Chromatogram

Start Injection

mAU

time

Page 8: Separation mechanism

8

Separations

Injector

Detector

Column

Solvents

Pumps

Mixer

Chromatogram

Start Injection

mAU

time

Page 9: Separation mechanism

9

Separations

Injector

Detector

Column

Solvents

Pumps

Mixer

Chromatogram

Start Injection

mAU

time

Page 10: Separation mechanism

10

Separations

Injector

Detector

Column

Solvents

Pumps

Mixer

Chromatogram

Start Injection

mAU

time

Page 11: Separation mechanism

11

Separations

Injector

Detector

Column

Solvents

Pumps

Mixer

Chromatogram

Start Injection

mAU

time

Page 12: Separation mechanism

12

Separations

Injector

Detector

Column

Solvents

Pumps

Mixer

Chromatogram

Start Injection

mAU

time

Page 13: Separation mechanism

13

Separations

Injector

Detector

Column

Solvents

Pumps

Mixer

Chromatogram

Start Injection

mAU

time

Page 14: Separation mechanism

14

Separations

Injector

Detector

Column

Solvents

Pumps

Mixer

Chromatogram

Start Injection

mAU

time

Page 15: Separation mechanism

15

Separations

Injector

Detector

Column

Solvents

Pumps

Mixer

Chromatogram

Start Injection

mAU

time

Page 16: Separation mechanism

16

Separations

Injector

Detector

Column

Solvents

Pumps

Mixer

Chromatogram

Start Injection

mAU

time

Page 17: Separation mechanism

17

Separations

Injector

Detector

Column

Solvents

Pumps

Mixer

Chromatogram

Start Injection

mAU

time

Page 18: Separation mechanism

18

HPLC Applications

Chemical

Environmental

Pharmaceuticals

Consumer Products

Clinical

polystyrenes

dyes

phthalates

tetracyclines

corticosteroids

antidepressants

barbiturates

amino acids

vitamins

homocysteine

Bioscience

proteins

peptides

nucleotides

lipids

antioxidants

sugars

polyaromatic hydrocarbons

Inorganic ions

herbicides


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