seminar2012 mothter safe
TRANSCRIPT
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Human EmbryologyHuman Embryology
제일병원 병리과 전이경
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Critical periods of development for various organ systems and the resultant
malformations
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First week of development: ovulation to implantation
blastocyst
morule
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4.5 days
6 days
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Second week of development:bilaminar germ disc
Week of twosWeek of twos
• Inner cell mass (embryoblast)– Epiblast – Hypoblast
• Trophoblast – Cytotrophoblast– Syncytiotrophoblast
• Extraembryonic mesoderm– Somatopleuric mesoderm– Splanchnopleuric
mesoderm• Two cavities
– Amniotic cavity– Yolk sac
• Uteroplacental circulation12 days
Amniotic cavity
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Day 13
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Third week of development: Trilaminar germ disc
Gastrulation• Bilamelar germ disc is
converted into a trilamelar germ– Ectoderm– Mesoderm – Endoderm
• Primitive streak– Thickened linear
band of epiblast– 15- to 16-day
embryo: clearly visible
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Epiblast ->
1.ectoderm
2.mesoderm
3.endoderm
18-day embryo
1.25 mm in length
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Derivatives of the ectodermal germ layer
1. Central nervous system2. Peripheral nervous system3. Skin, including hair and nails4. Sensory epithelium of ear, nose, and eye5. Pituitary, mammary, and sweat glands
and enamel of the teeth
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Derivatives of the mesodermal germ layer
• Connective tissue, cartilage, bone and striated and smooth muscles
• heart, blood and lymph vessels and cells
• kidney, ovary and testis, genital ducts, serous membranes lining, spleen, and adrenal cortex
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Derivatives of the endodermal germ layer
1. Epithelial lining of the gastrointestinal tract, respiratory tract, and urinary bladder
2. Parenchyma of the thyroid, parathyroid, liver and pancreas
3. Epithelial lining of the tympanic cavity and auditory tube
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Fate of the primitive streak
• intraembryonic mesoderm 을 4 주말까지 만들다가 감소
• insignificant structures in the sacrococcygeal region of the embryo
• Sacrococcygeal teratoma
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Teratogenesis associated with gastrulation
• The beginning of the third week of development, when gastrulation is initiated, is a highly sensitive stage for teratogenic insult.
• At this time, fate maps can be made for various organ systems, such as the eyes and brain anlage, and these cell populations may be damaged by teratogens.
• For example, high doses of alcohol at this stage kill cells in the anterior midline of the germ disc, producing a deficiency of the midline in craniofacial structures and resulting in holoprosencephaly.
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Caudal dysgenesis (sirenomelia)
• Insufficient mesoderm in the lumbosacral region of the embryo
− Hypoplasia and fusion of lower limb
− Vertebral abnormalities
− Renal agenesis
− Imperforate anus
− Anomalies of the genital organs
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Formation of the notochord
B C
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Notochord:1. Primitive axis of the embryo
2. Induction - neural plate (future nervous system)
Fate of the Notochord:– Regress in the vertebral bodies– Intervertebral disc: persist as
the nucleus pulposus
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20 days
Neurulation includes
the formation of the neural plate (day 18-19), neural folds (day 20-21), and the neural tube (day 22-26); the latter will develop into the future brain and spinal cord
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Final closure
Anterior neuropore: 25th day
Posterior neuropore: 27th day
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Neural tube defectsNeural tube defects• Most common CNS malformation,
1/1000 births • Primary non-close of the neural tube• Spectrum
– Anencephaly– Meningocele– Meningoencephalocele– Meningomyelocele
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AnencephalyAnencephaly• Absence of scalp,
calvarium, and normal brain -> “frog’s face”
• Area cerebrovasculosa• Hypoplastic adrenal
glands with absent fetal zone
• Recurrence rate: 3-5%• DDx: amniotic disruption
sequence
Amnion disruption
consequence
Anencephaly
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HoloprosencephHoloprosencephalyaly
4th week, 4mm, 3 vesicle stage 5th week, 8 mm, 5 vesicle stage
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LissencephLissencephalyaly
• “Smooth brain”• Severity : the absence
(agyria) to reduction (pachygyria) of normal gyral pattern.
• Classic L./cobblestone L.
• Onset : no later than the 12th–16th week of gestation
• DDx: immature GA (until 27-28W)
•GA 31 wks•46, XX, del(17)(p13)•“Miller-Dieker” syndrome
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Formation and migration of neural crest cells in the spinal cord
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Neural crest cells• Vulnerable cell population • Easily killed by compounds such as alcohol and retinoic
acid.• Deficient in superoxide dismutase and catalase enzymes
that are responsible for scavenging free radicals.• Neural crest derivativesNeural crest derivatives
Connective tissue and bones of the face and skull Dermis in face and neck
☞ Severe craniofacial malformations☞ Treacher-Collins' Syndrome, DiGeorge anomaly...
Conotruncal septum in the heart ☞ cardiac anomalies including persistent truncus arteriosus, TOF and TGA
Cranial nerve ganglia, spinal ganglia, sympathetic chain and preaortic ganglia, parasympathetic ganglia of the gastrointestinal tract, glial cells, schwann cells, adrenal medulla, C cells of the thyroid gland, arachnoid and pia mater, melanocytes, odontoblasts
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Neural crest cells in conotruncal region
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MIGRATION PATHWAYS OF NEURAL CREST CELLS from forebrain, midbrain, and hindbrain regions into their final locations (shaded areas) in the pharyngeal arches and face
Skeletal structures of the head and face. Mesenchyme for these structures is derived from neural crest (blue), lateral plate mesoderm (yellow), and paraxial mesoderm (red).
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Treacher-Collins' Syndrome / mandibulofacial dysostosis
• Characterized by malar hypoplasia due to underdevelopment of cheek bones, mandibular hypoplasia, down-slanting eyes and malformed external ears
• Normal development and intelligence
• AD with variable penetrance
• 60% as new mutation www.treachercollins.org/main.html
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Velocardiofacial Syndrome/DiGeorge anomaly
• 22q11.2 deletion• “CATCH 22”
– Cardiac defects– Abnormal face– Thymic hypoplasia – Cleft palate– Hypocalcemia
• Abnormal development of neural crest cells
• Specific facial features – low-set ears, wide-set
eyes, a small jaw, and a short groove in the upper lip
• Etiology– Genetic causes,
exposure to retinoic acids, alcohol, and maternal DM
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Limb growth and development
A. 5-week embryo B. 6-week embryo C. 8-week embryo
The hindlimb buds are less well developed than those of the forelimbs.
The most sensitive period for teratogen-induced limb malformations is the fourth and fifth weeks of development
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Nasal pit
Lateral nasal prominenceMedial nasal prominence
5 주
10주
7 주
6 주
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A.B. 6.5-week embryo The palatine shelves are in the vertical position on each side of the tongue.
C.D. 7.5-week embryo The tongue has moved downward, and the palatine shelves have reached a horizontal position.
E.F. 10-week embryo The two palatine shelves have fused with each other and with the nasal septum.
C D
FE
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A, day 18 B, day 20 C, day 21 D, day 22*
Heart tube
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Atrial Septation (4-6 weeks)
Septum primum
ostium primum
Interventricular foramen
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ostium secundum
Septumsecundum
35 days
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Development of conotruncal ridges and closure of the interventricular
foramen
6 weeks Beginning of 7 weeks
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End of 7 weeks
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Third to eighth week:The embryonic period
• Period of organogenesis
• Each of three germ layers gives rise to its own tissues and organs.
• Major features of the external body form recognizable by the end of the second month
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