Supporting information

Seeing the Diabetes Visual Detection of Glucose Based on the

Intrinsic Peroxidase-Like Activity of MoS2 Nanosheets

Tianran Lin Liangshuang Zhong Liangqia Guo Fengfu Fu and Guonan Chen

Ministry of Education Key Laboratory of Analysis and Detection for Food Safety

Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food

Safety and Department of Chemistry Fuzhou University Fuzhou Fujian 350108

China Fax +86 591-22866135 Tel +86 591-22866135 E-mail lqguofzueducn

Electronic Supplementary Material (ESI) for NanoscaleThis journal is copy The Royal Society of Chemistry 2014

J S K Mo L

5000 nm

Element Weight Atomic Mo L 1297 358S K 1026 847

G H

I

FigS2 The color changing with the increasing of reaction time after mixing MoS2

nanosheets (18 microg mL-1) with TMB (12 mmol L-1) and H2O2 (004 mmol L-1) in a

reaction volume of 05 mL Tris-HCl buffer (10 mmol L-1 pH 69)

0 min 1 min 3 min 5 min

20 min 30 min 45 min

13 min 15 min

7 min 9 min 11 min

FigS3 TEM image (A) HRTEM image (B) TEM-based EDX pattern (C) XPS Mo3d core-level spectrum (D) XPS S 2p core-level spectrum (E) AFM image (F) AFM 3D height profile (G) height profile along the white line shown in the AFM image(H) SEM-based EDX pattern (Inset are SEM image S and Mo mapping images) (I)of MoS2 nanosheets after catalysis reaction The signals of Cu C and O in TEM-based EDX and SEM-based EDX patterns (C I) originated from the carbon film supported by copper grids

100 nm100 nm 5 nm5 nm

1 nm1 nm

d=062nm

A B C

Cou

nts

Energy (kev)

240 238 236 234 232 230 228 226 224 222

Inte

nsit

y (a

u)

Binding Energy (eV)

Raw Intensity Peak Sum Background Mo 3d

32

Mo 3d52

S 2s

Mo 3d32

Mo 3d52

S 2s

176 172 168 164 160 156

Binding Energy (eV)

Inte

nsit

y (a

u)

Raw intensity Peak sum Background S 2p

32

S 2p12

S 2p12

S 2p32

D E

F G

H

I S K Mo L

2000 nm

Element Weight Atomic Mo L 1517 409S K 1185 955

FigS4 Photos of oxidation reaction of ABTS (20 mmol L-1) in Tris-HCl buffer (pH

70 10 mmol L-1) (A) and OPD (300 mmol L-1) in HAc-NaAc buffer (pH 40 200

mmol L-1) (B) in the presence of H2O2 (004 mmol L-1) (a) MoS2 (18 microg mL-1) (b)

and H2O2 (004 mmol L-1) + MoS2 (18 microg mL-1) (c) at room temperature after

reaction for 12 min

B

A

a b c

a b c

0 100 200 300 400 500 60000

01

02

03

04

05

06

07

08

Abs

Time s

180 144108072036

MoS2 (ug mL-1)

0

FigS5 The time-dependent absorbance changes at 652 nm in the presence of

different concentrations of MoS2 nanosheets in Tris-HCl buffer (10 mmol L-1 pH 69)

at 30 degC

FigS6 Effect of pH (A) temperature (B) H2O2 concentration (C) and reaction time

(D) on the peroxidase-like activity of MoS2 nanosheets for the TMB oxidation The

experiment was carried out using 18 microg mL-1 MoS2 nanosheets in a reaction volume

of 05 mL in Tris-HCl buffer (10 mmol L-1 pH 69) with 12 mmol L-1 TMB and 004

mmol L-1 H2O2 for 30 min at 30 degC The error bars represent the standard deviation of

three measurements

0 300 600 900 1200 1500 180001

02

03

04

05

06

07

08

09

Abs

Time s

0 1 2 3 4 5 6 7 8 9

00

02

04

06

08

10

12

14

16

18

Abs

pH20 30 40 50 60 70

06

07

08

09

10

11

12

13

14

15

16

17

Abs

Temperature

0 2000 4000 6000 8000 10000

00

05

10

15

20

25

Abs

H2O

2 mmol L-1

A B

C D

FigS7 Steady-state kinetic assay of HRP The velocity (v) of the reaction was

measured using HRP (5 ng L-1) in 05 mL Tris-HCl buffer (10 mmol L-1 pH 69) at

30 (A) The concentration of TMB was 12 mmol L-1 and H2O2 concentration was

varied (B) The concentration of H2O2 was 32 mmol L-1 and TMB concentration was

varied

0000 0002 0004 0006 00080

5

10

15

20

25

30

H2O

2 mol L-1

v10

-8m

olL

-1s-1

01 02 03 04 05

5

10

15

20

25

30

35

v10

-8m

olL

-1s-1

TMBmmol L-1

A B

300 350 400 450 500 550 600 650 700

00

01

02

03

04

05

Abs

Wavelength (nm)

1

2

3

4

5

6

7

FigS8 Absorption spectra of TMB (1) MoS2 nanosheets (7) and a fixed concentration of TMB interacting with different concentrations of MoS2 nanosheets (2-6)

(1) 50 microL TMB (12 mmol L-1) + 50 microL H2O + 200 microL Tris-HCl (pH 69 10 mmol L-1)

+ 200 microL H2O

(2) 50 microL TMB (12 mmol L-1) + 10microL MoS2 (18 microg mL-1) + 40 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(3) 50 microL TMB (12 mmol L-1) + 20 microL MoS2 (18microg mL-1) + 30 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(4) 50 microL TMB (12 mmol L-1) + 30 microL MoS2 (18 microg mL-1) + 20 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(5) 50 microL TMB (12 mmol L-1) + 40 microL MoS2 (18 microg mL-1) + 10 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(6) 50 microL TMB (12 mmol L-1) + 50 microL MoS2 (18 microg mL-1) + 200 microL Tris-HCl (pH69

10 mmol L-1) + 200 microL H2O

(7) 50 microL H2O + 50 microL MoS2 (18 microg mL-1) + 200 microL Tris-HCl (pH6910 mmol L-1) +

200 microL H2O

00

02

04

06

08

10

12

14

Abs

5μL 10μL 15μL 20μL 25μL 30μL

control NaN3

ascorbic acid

thioureaSOD

FigS9 The catalytic reaction at the presence of different radical scavengers

Procedures 50 μL MoS2 (18 μg mL-1) 200 μL Tris-HCl (69 10 mmol L-1) 50 μL

TMB (12 mmol L-1) and different volumes of radical scavenger were mixed The

volume of the mixture was adjusted to 400 μL with water then 200 μL H2O2 (01

mmol L-1) was added After the reaction was carried out at room temperature for 30

min 20 μL H2SO4 (vv20) was added into the above mixture The absorbance at

450 nm was recorded The original concentrations of NaN3 ascorbic acid thiourea

and SOD were 1 mmol L-1 1 mmol L-1 1 mmol L- and 20 U mL-1 respectively

00

02

04

06

08

10

12

14

16

18

Abs

375μM 75μM 1125μM

control ClO- middotOH H2O

2

1O2

middotO2

-

Fig S10The response of this catalytic reaction to various ROSProcedures 50 μL MoS2 (18 μg mL-1) 200 μL Tris-HCl (69 10 mmol L-1) 50 μL TMB (12 mmol L-1) and 200 μL ROS were mixed After the reaction was carried out at room temperature for 30 min The absorbance at 652 nm was recorded

The generation of ROS was performed using documented protocols (Abo et al 2011 J Am Chem Soc 133 10629-10637 Chen et al 2013 J Am Chem Soc 135 11595-11602 Lee et al 2009 Adv Funct Mater 19(12) 1884-1890) Hydroxyl radical (OH) was generated by the Fenton reaction between H2O2 and ferrous sulphate at a molar concentration ratio of 101 Hypochlorite (ClO-) and superoxide anion (O2

-) were obtained from NaOCl and KO2 respectively The singlet oxygen (1O2) was produced by H2O2 with NaClO The absorbance was enhanced greatly at the presence of OH ClO- as well as H2O2

FigS11 Selectivity analysis for glucose detection by monitoring the relative

absorbance Inset of FigS9 were images of colored production for the different

solutions ((a) 5 mmol L-1 fructose (b) 5 mmol L-1 lactose (c) 025 mmol L-1 maltose

(d) 005 mmol L-1 glucose and blank) The error bars represent the standard deviation

of three measurements

00

01

02

03

04

05

06

A-A

0

fructose

5 mmol L-1lactose

5 mmol L-1

maltose

025 mmol L-1

glucose

005 mmol L-1

a b c d

a b c d

e

e

Table S1 Comparison of the apparent Michaelies-Menten constant (Km) and

maximum reaction rate (Vmax) between MoS2 and HRP Km is the Michaelies constant

Vmax is the maximal reaction velocity

Catalyst substance Km (mmol L-1) Vmax (mol L-1s)

MoS2 H2O2 00116 429times10-8

MoS2 TMB 0525 516times10-8

HRP H2O2 109 585times10-8

HRP TMB 0172 418times10-8

Table S2 Results of determination of glucose in serum samples

Glucose meter method a

(mmol L-1)

Proposed method b

(mmol L-1)

RSD

()

Serum 1 500 453plusmn002 c -94

Serum 2 667 631plusmn001 c -54

Serum 3 833 779plusmn001 c -65

a The glucose determination was performed by the conventional enzymatic method at

the First Affiliated Hospital of Fujian Medical Universityb The confidence level was 95c n=3

J S K Mo L

5000 nm

Element Weight Atomic Mo L 1297 358S K 1026 847

G H

I

FigS2 The color changing with the increasing of reaction time after mixing MoS2

nanosheets (18 microg mL-1) with TMB (12 mmol L-1) and H2O2 (004 mmol L-1) in a

reaction volume of 05 mL Tris-HCl buffer (10 mmol L-1 pH 69)

0 min 1 min 3 min 5 min

20 min 30 min 45 min

13 min 15 min

7 min 9 min 11 min

FigS3 TEM image (A) HRTEM image (B) TEM-based EDX pattern (C) XPS Mo3d core-level spectrum (D) XPS S 2p core-level spectrum (E) AFM image (F) AFM 3D height profile (G) height profile along the white line shown in the AFM image(H) SEM-based EDX pattern (Inset are SEM image S and Mo mapping images) (I)of MoS2 nanosheets after catalysis reaction The signals of Cu C and O in TEM-based EDX and SEM-based EDX patterns (C I) originated from the carbon film supported by copper grids

100 nm100 nm 5 nm5 nm

1 nm1 nm

d=062nm

A B C

Cou

nts

Energy (kev)

240 238 236 234 232 230 228 226 224 222

Inte

nsit

y (a

u)

Binding Energy (eV)

Raw Intensity Peak Sum Background Mo 3d

32

Mo 3d52

S 2s

Mo 3d32

Mo 3d52

S 2s

176 172 168 164 160 156

Binding Energy (eV)

Inte

nsit

y (a

u)

Raw intensity Peak sum Background S 2p

32

S 2p12

S 2p12

S 2p32

D E

F G

H

I S K Mo L

2000 nm

Element Weight Atomic Mo L 1517 409S K 1185 955

FigS4 Photos of oxidation reaction of ABTS (20 mmol L-1) in Tris-HCl buffer (pH

70 10 mmol L-1) (A) and OPD (300 mmol L-1) in HAc-NaAc buffer (pH 40 200

mmol L-1) (B) in the presence of H2O2 (004 mmol L-1) (a) MoS2 (18 microg mL-1) (b)

and H2O2 (004 mmol L-1) + MoS2 (18 microg mL-1) (c) at room temperature after

reaction for 12 min

B

A

a b c

a b c

0 100 200 300 400 500 60000

01

02

03

04

05

06

07

08

Abs

Time s

180 144108072036

MoS2 (ug mL-1)

0

FigS5 The time-dependent absorbance changes at 652 nm in the presence of

different concentrations of MoS2 nanosheets in Tris-HCl buffer (10 mmol L-1 pH 69)

at 30 degC

FigS6 Effect of pH (A) temperature (B) H2O2 concentration (C) and reaction time

(D) on the peroxidase-like activity of MoS2 nanosheets for the TMB oxidation The

experiment was carried out using 18 microg mL-1 MoS2 nanosheets in a reaction volume

of 05 mL in Tris-HCl buffer (10 mmol L-1 pH 69) with 12 mmol L-1 TMB and 004

mmol L-1 H2O2 for 30 min at 30 degC The error bars represent the standard deviation of

three measurements

0 300 600 900 1200 1500 180001

02

03

04

05

06

07

08

09

Abs

Time s

0 1 2 3 4 5 6 7 8 9

00

02

04

06

08

10

12

14

16

18

Abs

pH20 30 40 50 60 70

06

07

08

09

10

11

12

13

14

15

16

17

Abs

Temperature

0 2000 4000 6000 8000 10000

00

05

10

15

20

25

Abs

H2O

2 mmol L-1

A B

C D

FigS7 Steady-state kinetic assay of HRP The velocity (v) of the reaction was

measured using HRP (5 ng L-1) in 05 mL Tris-HCl buffer (10 mmol L-1 pH 69) at

30 (A) The concentration of TMB was 12 mmol L-1 and H2O2 concentration was

varied (B) The concentration of H2O2 was 32 mmol L-1 and TMB concentration was

varied

0000 0002 0004 0006 00080

5

10

15

20

25

30

H2O

2 mol L-1

v10

-8m

olL

-1s-1

01 02 03 04 05

5

10

15

20

25

30

35

v10

-8m

olL

-1s-1

TMBmmol L-1

A B

300 350 400 450 500 550 600 650 700

00

01

02

03

04

05

Abs

Wavelength (nm)

1

2

3

4

5

6

7

FigS8 Absorption spectra of TMB (1) MoS2 nanosheets (7) and a fixed concentration of TMB interacting with different concentrations of MoS2 nanosheets (2-6)

(1) 50 microL TMB (12 mmol L-1) + 50 microL H2O + 200 microL Tris-HCl (pH 69 10 mmol L-1)

+ 200 microL H2O

(2) 50 microL TMB (12 mmol L-1) + 10microL MoS2 (18 microg mL-1) + 40 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(3) 50 microL TMB (12 mmol L-1) + 20 microL MoS2 (18microg mL-1) + 30 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(4) 50 microL TMB (12 mmol L-1) + 30 microL MoS2 (18 microg mL-1) + 20 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(5) 50 microL TMB (12 mmol L-1) + 40 microL MoS2 (18 microg mL-1) + 10 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(6) 50 microL TMB (12 mmol L-1) + 50 microL MoS2 (18 microg mL-1) + 200 microL Tris-HCl (pH69

10 mmol L-1) + 200 microL H2O

(7) 50 microL H2O + 50 microL MoS2 (18 microg mL-1) + 200 microL Tris-HCl (pH6910 mmol L-1) +

200 microL H2O

00

02

04

06

08

10

12

14

Abs

5μL 10μL 15μL 20μL 25μL 30μL

control NaN3

ascorbic acid

thioureaSOD

FigS9 The catalytic reaction at the presence of different radical scavengers

Procedures 50 μL MoS2 (18 μg mL-1) 200 μL Tris-HCl (69 10 mmol L-1) 50 μL

TMB (12 mmol L-1) and different volumes of radical scavenger were mixed The

volume of the mixture was adjusted to 400 μL with water then 200 μL H2O2 (01

mmol L-1) was added After the reaction was carried out at room temperature for 30

min 20 μL H2SO4 (vv20) was added into the above mixture The absorbance at

450 nm was recorded The original concentrations of NaN3 ascorbic acid thiourea

and SOD were 1 mmol L-1 1 mmol L-1 1 mmol L- and 20 U mL-1 respectively

00

02

04

06

08

10

12

14

16

18

Abs

375μM 75μM 1125μM

control ClO- middotOH H2O

2

1O2

middotO2

-

Fig S10The response of this catalytic reaction to various ROSProcedures 50 μL MoS2 (18 μg mL-1) 200 μL Tris-HCl (69 10 mmol L-1) 50 μL TMB (12 mmol L-1) and 200 μL ROS were mixed After the reaction was carried out at room temperature for 30 min The absorbance at 652 nm was recorded

The generation of ROS was performed using documented protocols (Abo et al 2011 J Am Chem Soc 133 10629-10637 Chen et al 2013 J Am Chem Soc 135 11595-11602 Lee et al 2009 Adv Funct Mater 19(12) 1884-1890) Hydroxyl radical (OH) was generated by the Fenton reaction between H2O2 and ferrous sulphate at a molar concentration ratio of 101 Hypochlorite (ClO-) and superoxide anion (O2

-) were obtained from NaOCl and KO2 respectively The singlet oxygen (1O2) was produced by H2O2 with NaClO The absorbance was enhanced greatly at the presence of OH ClO- as well as H2O2

FigS11 Selectivity analysis for glucose detection by monitoring the relative

absorbance Inset of FigS9 were images of colored production for the different

solutions ((a) 5 mmol L-1 fructose (b) 5 mmol L-1 lactose (c) 025 mmol L-1 maltose

(d) 005 mmol L-1 glucose and blank) The error bars represent the standard deviation

of three measurements

00

01

02

03

04

05

06

A-A

0

fructose

5 mmol L-1lactose

5 mmol L-1

maltose

025 mmol L-1

glucose

005 mmol L-1

a b c d

a b c d

e

e

Table S1 Comparison of the apparent Michaelies-Menten constant (Km) and

maximum reaction rate (Vmax) between MoS2 and HRP Km is the Michaelies constant

Vmax is the maximal reaction velocity

Catalyst substance Km (mmol L-1) Vmax (mol L-1s)

MoS2 H2O2 00116 429times10-8

MoS2 TMB 0525 516times10-8

HRP H2O2 109 585times10-8

HRP TMB 0172 418times10-8

Table S2 Results of determination of glucose in serum samples

Glucose meter method a

(mmol L-1)

Proposed method b

(mmol L-1)

RSD

()

Serum 1 500 453plusmn002 c -94

Serum 2 667 631plusmn001 c -54

Serum 3 833 779plusmn001 c -65

a The glucose determination was performed by the conventional enzymatic method at

the First Affiliated Hospital of Fujian Medical Universityb The confidence level was 95c n=3

FigS2 The color changing with the increasing of reaction time after mixing MoS2

nanosheets (18 microg mL-1) with TMB (12 mmol L-1) and H2O2 (004 mmol L-1) in a

reaction volume of 05 mL Tris-HCl buffer (10 mmol L-1 pH 69)

0 min 1 min 3 min 5 min

20 min 30 min 45 min

13 min 15 min

7 min 9 min 11 min

FigS3 TEM image (A) HRTEM image (B) TEM-based EDX pattern (C) XPS Mo3d core-level spectrum (D) XPS S 2p core-level spectrum (E) AFM image (F) AFM 3D height profile (G) height profile along the white line shown in the AFM image(H) SEM-based EDX pattern (Inset are SEM image S and Mo mapping images) (I)of MoS2 nanosheets after catalysis reaction The signals of Cu C and O in TEM-based EDX and SEM-based EDX patterns (C I) originated from the carbon film supported by copper grids

100 nm100 nm 5 nm5 nm

1 nm1 nm

d=062nm

A B C

Cou

nts

Energy (kev)

240 238 236 234 232 230 228 226 224 222

Inte

nsit

y (a

u)

Binding Energy (eV)

Raw Intensity Peak Sum Background Mo 3d

32

Mo 3d52

S 2s

Mo 3d32

Mo 3d52

S 2s

176 172 168 164 160 156

Binding Energy (eV)

Inte

nsit

y (a

u)

Raw intensity Peak sum Background S 2p

32

S 2p12

S 2p12

S 2p32

D E

F G

H

I S K Mo L

2000 nm

Element Weight Atomic Mo L 1517 409S K 1185 955

FigS4 Photos of oxidation reaction of ABTS (20 mmol L-1) in Tris-HCl buffer (pH

70 10 mmol L-1) (A) and OPD (300 mmol L-1) in HAc-NaAc buffer (pH 40 200

mmol L-1) (B) in the presence of H2O2 (004 mmol L-1) (a) MoS2 (18 microg mL-1) (b)

and H2O2 (004 mmol L-1) + MoS2 (18 microg mL-1) (c) at room temperature after

reaction for 12 min

B

A

a b c

a b c

0 100 200 300 400 500 60000

01

02

03

04

05

06

07

08

Abs

Time s

180 144108072036

MoS2 (ug mL-1)

0

FigS5 The time-dependent absorbance changes at 652 nm in the presence of

different concentrations of MoS2 nanosheets in Tris-HCl buffer (10 mmol L-1 pH 69)

at 30 degC

FigS6 Effect of pH (A) temperature (B) H2O2 concentration (C) and reaction time

(D) on the peroxidase-like activity of MoS2 nanosheets for the TMB oxidation The

experiment was carried out using 18 microg mL-1 MoS2 nanosheets in a reaction volume

of 05 mL in Tris-HCl buffer (10 mmol L-1 pH 69) with 12 mmol L-1 TMB and 004

mmol L-1 H2O2 for 30 min at 30 degC The error bars represent the standard deviation of

three measurements

0 300 600 900 1200 1500 180001

02

03

04

05

06

07

08

09

Abs

Time s

0 1 2 3 4 5 6 7 8 9

00

02

04

06

08

10

12

14

16

18

Abs

pH20 30 40 50 60 70

06

07

08

09

10

11

12

13

14

15

16

17

Abs

Temperature

0 2000 4000 6000 8000 10000

00

05

10

15

20

25

Abs

H2O

2 mmol L-1

A B

C D

FigS7 Steady-state kinetic assay of HRP The velocity (v) of the reaction was

measured using HRP (5 ng L-1) in 05 mL Tris-HCl buffer (10 mmol L-1 pH 69) at

30 (A) The concentration of TMB was 12 mmol L-1 and H2O2 concentration was

varied (B) The concentration of H2O2 was 32 mmol L-1 and TMB concentration was

varied

0000 0002 0004 0006 00080

5

10

15

20

25

30

H2O

2 mol L-1

v10

-8m

olL

-1s-1

01 02 03 04 05

5

10

15

20

25

30

35

v10

-8m

olL

-1s-1

TMBmmol L-1

A B

300 350 400 450 500 550 600 650 700

00

01

02

03

04

05

Abs

Wavelength (nm)

1

2

3

4

5

6

7

FigS8 Absorption spectra of TMB (1) MoS2 nanosheets (7) and a fixed concentration of TMB interacting with different concentrations of MoS2 nanosheets (2-6)

(1) 50 microL TMB (12 mmol L-1) + 50 microL H2O + 200 microL Tris-HCl (pH 69 10 mmol L-1)

+ 200 microL H2O

(2) 50 microL TMB (12 mmol L-1) + 10microL MoS2 (18 microg mL-1) + 40 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(3) 50 microL TMB (12 mmol L-1) + 20 microL MoS2 (18microg mL-1) + 30 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(4) 50 microL TMB (12 mmol L-1) + 30 microL MoS2 (18 microg mL-1) + 20 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(5) 50 microL TMB (12 mmol L-1) + 40 microL MoS2 (18 microg mL-1) + 10 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(6) 50 microL TMB (12 mmol L-1) + 50 microL MoS2 (18 microg mL-1) + 200 microL Tris-HCl (pH69

10 mmol L-1) + 200 microL H2O

(7) 50 microL H2O + 50 microL MoS2 (18 microg mL-1) + 200 microL Tris-HCl (pH6910 mmol L-1) +

200 microL H2O

00

02

04

06

08

10

12

14

Abs

5μL 10μL 15μL 20μL 25μL 30μL

control NaN3

ascorbic acid

thioureaSOD

FigS9 The catalytic reaction at the presence of different radical scavengers

Procedures 50 μL MoS2 (18 μg mL-1) 200 μL Tris-HCl (69 10 mmol L-1) 50 μL

TMB (12 mmol L-1) and different volumes of radical scavenger were mixed The

volume of the mixture was adjusted to 400 μL with water then 200 μL H2O2 (01

mmol L-1) was added After the reaction was carried out at room temperature for 30

min 20 μL H2SO4 (vv20) was added into the above mixture The absorbance at

450 nm was recorded The original concentrations of NaN3 ascorbic acid thiourea

and SOD were 1 mmol L-1 1 mmol L-1 1 mmol L- and 20 U mL-1 respectively

00

02

04

06

08

10

12

14

16

18

Abs

375μM 75μM 1125μM

control ClO- middotOH H2O

2

1O2

middotO2

-

Fig S10The response of this catalytic reaction to various ROSProcedures 50 μL MoS2 (18 μg mL-1) 200 μL Tris-HCl (69 10 mmol L-1) 50 μL TMB (12 mmol L-1) and 200 μL ROS were mixed After the reaction was carried out at room temperature for 30 min The absorbance at 652 nm was recorded

The generation of ROS was performed using documented protocols (Abo et al 2011 J Am Chem Soc 133 10629-10637 Chen et al 2013 J Am Chem Soc 135 11595-11602 Lee et al 2009 Adv Funct Mater 19(12) 1884-1890) Hydroxyl radical (OH) was generated by the Fenton reaction between H2O2 and ferrous sulphate at a molar concentration ratio of 101 Hypochlorite (ClO-) and superoxide anion (O2

-) were obtained from NaOCl and KO2 respectively The singlet oxygen (1O2) was produced by H2O2 with NaClO The absorbance was enhanced greatly at the presence of OH ClO- as well as H2O2

FigS11 Selectivity analysis for glucose detection by monitoring the relative

absorbance Inset of FigS9 were images of colored production for the different

solutions ((a) 5 mmol L-1 fructose (b) 5 mmol L-1 lactose (c) 025 mmol L-1 maltose

(d) 005 mmol L-1 glucose and blank) The error bars represent the standard deviation

of three measurements

00

01

02

03

04

05

06

A-A

0

fructose

5 mmol L-1lactose

5 mmol L-1

maltose

025 mmol L-1

glucose

005 mmol L-1

a b c d

a b c d

e

e

Table S1 Comparison of the apparent Michaelies-Menten constant (Km) and

maximum reaction rate (Vmax) between MoS2 and HRP Km is the Michaelies constant

Vmax is the maximal reaction velocity

Catalyst substance Km (mmol L-1) Vmax (mol L-1s)

MoS2 H2O2 00116 429times10-8

MoS2 TMB 0525 516times10-8

HRP H2O2 109 585times10-8

HRP TMB 0172 418times10-8

Table S2 Results of determination of glucose in serum samples

Glucose meter method a

(mmol L-1)

Proposed method b

(mmol L-1)

RSD

()

Serum 1 500 453plusmn002 c -94

Serum 2 667 631plusmn001 c -54

Serum 3 833 779plusmn001 c -65

a The glucose determination was performed by the conventional enzymatic method at

the First Affiliated Hospital of Fujian Medical Universityb The confidence level was 95c n=3

FigS3 TEM image (A) HRTEM image (B) TEM-based EDX pattern (C) XPS Mo3d core-level spectrum (D) XPS S 2p core-level spectrum (E) AFM image (F) AFM 3D height profile (G) height profile along the white line shown in the AFM image(H) SEM-based EDX pattern (Inset are SEM image S and Mo mapping images) (I)of MoS2 nanosheets after catalysis reaction The signals of Cu C and O in TEM-based EDX and SEM-based EDX patterns (C I) originated from the carbon film supported by copper grids

100 nm100 nm 5 nm5 nm

1 nm1 nm

d=062nm

A B C

Cou

nts

Energy (kev)

240 238 236 234 232 230 228 226 224 222

Inte

nsit

y (a

u)

Binding Energy (eV)

Raw Intensity Peak Sum Background Mo 3d

32

Mo 3d52

S 2s

Mo 3d32

Mo 3d52

S 2s

176 172 168 164 160 156

Binding Energy (eV)

Inte

nsit

y (a

u)

Raw intensity Peak sum Background S 2p

32

S 2p12

S 2p12

S 2p32

D E

F G

H

I S K Mo L

2000 nm

Element Weight Atomic Mo L 1517 409S K 1185 955

FigS4 Photos of oxidation reaction of ABTS (20 mmol L-1) in Tris-HCl buffer (pH

70 10 mmol L-1) (A) and OPD (300 mmol L-1) in HAc-NaAc buffer (pH 40 200

mmol L-1) (B) in the presence of H2O2 (004 mmol L-1) (a) MoS2 (18 microg mL-1) (b)

and H2O2 (004 mmol L-1) + MoS2 (18 microg mL-1) (c) at room temperature after

reaction for 12 min

B

A

a b c

a b c

0 100 200 300 400 500 60000

01

02

03

04

05

06

07

08

Abs

Time s

180 144108072036

MoS2 (ug mL-1)

0

FigS5 The time-dependent absorbance changes at 652 nm in the presence of

different concentrations of MoS2 nanosheets in Tris-HCl buffer (10 mmol L-1 pH 69)

at 30 degC

FigS6 Effect of pH (A) temperature (B) H2O2 concentration (C) and reaction time

(D) on the peroxidase-like activity of MoS2 nanosheets for the TMB oxidation The

experiment was carried out using 18 microg mL-1 MoS2 nanosheets in a reaction volume

of 05 mL in Tris-HCl buffer (10 mmol L-1 pH 69) with 12 mmol L-1 TMB and 004

mmol L-1 H2O2 for 30 min at 30 degC The error bars represent the standard deviation of

three measurements

0 300 600 900 1200 1500 180001

02

03

04

05

06

07

08

09

Abs

Time s

0 1 2 3 4 5 6 7 8 9

00

02

04

06

08

10

12

14

16

18

Abs

pH20 30 40 50 60 70

06

07

08

09

10

11

12

13

14

15

16

17

Abs

Temperature

0 2000 4000 6000 8000 10000

00

05

10

15

20

25

Abs

H2O

2 mmol L-1

A B

C D

FigS7 Steady-state kinetic assay of HRP The velocity (v) of the reaction was

measured using HRP (5 ng L-1) in 05 mL Tris-HCl buffer (10 mmol L-1 pH 69) at

30 (A) The concentration of TMB was 12 mmol L-1 and H2O2 concentration was

varied (B) The concentration of H2O2 was 32 mmol L-1 and TMB concentration was

varied

0000 0002 0004 0006 00080

5

10

15

20

25

30

H2O

2 mol L-1

v10

-8m

olL

-1s-1

01 02 03 04 05

5

10

15

20

25

30

35

v10

-8m

olL

-1s-1

TMBmmol L-1

A B

300 350 400 450 500 550 600 650 700

00

01

02

03

04

05

Abs

Wavelength (nm)

1

2

3

4

5

6

7

FigS8 Absorption spectra of TMB (1) MoS2 nanosheets (7) and a fixed concentration of TMB interacting with different concentrations of MoS2 nanosheets (2-6)

(1) 50 microL TMB (12 mmol L-1) + 50 microL H2O + 200 microL Tris-HCl (pH 69 10 mmol L-1)

+ 200 microL H2O

(2) 50 microL TMB (12 mmol L-1) + 10microL MoS2 (18 microg mL-1) + 40 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(3) 50 microL TMB (12 mmol L-1) + 20 microL MoS2 (18microg mL-1) + 30 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(4) 50 microL TMB (12 mmol L-1) + 30 microL MoS2 (18 microg mL-1) + 20 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(5) 50 microL TMB (12 mmol L-1) + 40 microL MoS2 (18 microg mL-1) + 10 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(6) 50 microL TMB (12 mmol L-1) + 50 microL MoS2 (18 microg mL-1) + 200 microL Tris-HCl (pH69

10 mmol L-1) + 200 microL H2O

(7) 50 microL H2O + 50 microL MoS2 (18 microg mL-1) + 200 microL Tris-HCl (pH6910 mmol L-1) +

200 microL H2O

00

02

04

06

08

10

12

14

Abs

5μL 10μL 15μL 20μL 25μL 30μL

control NaN3

ascorbic acid

thioureaSOD

FigS9 The catalytic reaction at the presence of different radical scavengers

Procedures 50 μL MoS2 (18 μg mL-1) 200 μL Tris-HCl (69 10 mmol L-1) 50 μL

TMB (12 mmol L-1) and different volumes of radical scavenger were mixed The

volume of the mixture was adjusted to 400 μL with water then 200 μL H2O2 (01

mmol L-1) was added After the reaction was carried out at room temperature for 30

min 20 μL H2SO4 (vv20) was added into the above mixture The absorbance at

450 nm was recorded The original concentrations of NaN3 ascorbic acid thiourea

and SOD were 1 mmol L-1 1 mmol L-1 1 mmol L- and 20 U mL-1 respectively

00

02

04

06

08

10

12

14

16

18

Abs

375μM 75μM 1125μM

control ClO- middotOH H2O

2

1O2

middotO2

-

Fig S10The response of this catalytic reaction to various ROSProcedures 50 μL MoS2 (18 μg mL-1) 200 μL Tris-HCl (69 10 mmol L-1) 50 μL TMB (12 mmol L-1) and 200 μL ROS were mixed After the reaction was carried out at room temperature for 30 min The absorbance at 652 nm was recorded

The generation of ROS was performed using documented protocols (Abo et al 2011 J Am Chem Soc 133 10629-10637 Chen et al 2013 J Am Chem Soc 135 11595-11602 Lee et al 2009 Adv Funct Mater 19(12) 1884-1890) Hydroxyl radical (OH) was generated by the Fenton reaction between H2O2 and ferrous sulphate at a molar concentration ratio of 101 Hypochlorite (ClO-) and superoxide anion (O2

-) were obtained from NaOCl and KO2 respectively The singlet oxygen (1O2) was produced by H2O2 with NaClO The absorbance was enhanced greatly at the presence of OH ClO- as well as H2O2

FigS11 Selectivity analysis for glucose detection by monitoring the relative

absorbance Inset of FigS9 were images of colored production for the different

solutions ((a) 5 mmol L-1 fructose (b) 5 mmol L-1 lactose (c) 025 mmol L-1 maltose

(d) 005 mmol L-1 glucose and blank) The error bars represent the standard deviation

of three measurements

00

01

02

03

04

05

06

A-A

0

fructose

5 mmol L-1lactose

5 mmol L-1

maltose

025 mmol L-1

glucose

005 mmol L-1

a b c d

a b c d

e

e

Table S1 Comparison of the apparent Michaelies-Menten constant (Km) and

maximum reaction rate (Vmax) between MoS2 and HRP Km is the Michaelies constant

Vmax is the maximal reaction velocity

Catalyst substance Km (mmol L-1) Vmax (mol L-1s)

MoS2 H2O2 00116 429times10-8

MoS2 TMB 0525 516times10-8

HRP H2O2 109 585times10-8

HRP TMB 0172 418times10-8

Table S2 Results of determination of glucose in serum samples

Glucose meter method a

(mmol L-1)

Proposed method b

(mmol L-1)

RSD

()

Serum 1 500 453plusmn002 c -94

Serum 2 667 631plusmn001 c -54

Serum 3 833 779plusmn001 c -65

a The glucose determination was performed by the conventional enzymatic method at

the First Affiliated Hospital of Fujian Medical Universityb The confidence level was 95c n=3

FigS4 Photos of oxidation reaction of ABTS (20 mmol L-1) in Tris-HCl buffer (pH

70 10 mmol L-1) (A) and OPD (300 mmol L-1) in HAc-NaAc buffer (pH 40 200

mmol L-1) (B) in the presence of H2O2 (004 mmol L-1) (a) MoS2 (18 microg mL-1) (b)

and H2O2 (004 mmol L-1) + MoS2 (18 microg mL-1) (c) at room temperature after

reaction for 12 min

B

A

a b c

a b c

0 100 200 300 400 500 60000

01

02

03

04

05

06

07

08

Abs

Time s

180 144108072036

MoS2 (ug mL-1)

0

FigS5 The time-dependent absorbance changes at 652 nm in the presence of

different concentrations of MoS2 nanosheets in Tris-HCl buffer (10 mmol L-1 pH 69)

at 30 degC

FigS6 Effect of pH (A) temperature (B) H2O2 concentration (C) and reaction time

(D) on the peroxidase-like activity of MoS2 nanosheets for the TMB oxidation The

experiment was carried out using 18 microg mL-1 MoS2 nanosheets in a reaction volume

of 05 mL in Tris-HCl buffer (10 mmol L-1 pH 69) with 12 mmol L-1 TMB and 004

mmol L-1 H2O2 for 30 min at 30 degC The error bars represent the standard deviation of

three measurements

0 300 600 900 1200 1500 180001

02

03

04

05

06

07

08

09

Abs

Time s

0 1 2 3 4 5 6 7 8 9

00

02

04

06

08

10

12

14

16

18

Abs

pH20 30 40 50 60 70

06

07

08

09

10

11

12

13

14

15

16

17

Abs

Temperature

0 2000 4000 6000 8000 10000

00

05

10

15

20

25

Abs

H2O

2 mmol L-1

A B

C D

FigS7 Steady-state kinetic assay of HRP The velocity (v) of the reaction was

measured using HRP (5 ng L-1) in 05 mL Tris-HCl buffer (10 mmol L-1 pH 69) at

30 (A) The concentration of TMB was 12 mmol L-1 and H2O2 concentration was

varied (B) The concentration of H2O2 was 32 mmol L-1 and TMB concentration was

varied

0000 0002 0004 0006 00080

5

10

15

20

25

30

H2O

2 mol L-1

v10

-8m

olL

-1s-1

01 02 03 04 05

5

10

15

20

25

30

35

v10

-8m

olL

-1s-1

TMBmmol L-1

A B

300 350 400 450 500 550 600 650 700

00

01

02

03

04

05

Abs

Wavelength (nm)

1

2

3

4

5

6

7

FigS8 Absorption spectra of TMB (1) MoS2 nanosheets (7) and a fixed concentration of TMB interacting with different concentrations of MoS2 nanosheets (2-6)

(1) 50 microL TMB (12 mmol L-1) + 50 microL H2O + 200 microL Tris-HCl (pH 69 10 mmol L-1)

+ 200 microL H2O

(2) 50 microL TMB (12 mmol L-1) + 10microL MoS2 (18 microg mL-1) + 40 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(3) 50 microL TMB (12 mmol L-1) + 20 microL MoS2 (18microg mL-1) + 30 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(4) 50 microL TMB (12 mmol L-1) + 30 microL MoS2 (18 microg mL-1) + 20 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(5) 50 microL TMB (12 mmol L-1) + 40 microL MoS2 (18 microg mL-1) + 10 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(6) 50 microL TMB (12 mmol L-1) + 50 microL MoS2 (18 microg mL-1) + 200 microL Tris-HCl (pH69

10 mmol L-1) + 200 microL H2O

(7) 50 microL H2O + 50 microL MoS2 (18 microg mL-1) + 200 microL Tris-HCl (pH6910 mmol L-1) +

200 microL H2O

00

02

04

06

08

10

12

14

Abs

5μL 10μL 15μL 20μL 25μL 30μL

control NaN3

ascorbic acid

thioureaSOD

FigS9 The catalytic reaction at the presence of different radical scavengers

Procedures 50 μL MoS2 (18 μg mL-1) 200 μL Tris-HCl (69 10 mmol L-1) 50 μL

TMB (12 mmol L-1) and different volumes of radical scavenger were mixed The

volume of the mixture was adjusted to 400 μL with water then 200 μL H2O2 (01

mmol L-1) was added After the reaction was carried out at room temperature for 30

min 20 μL H2SO4 (vv20) was added into the above mixture The absorbance at

450 nm was recorded The original concentrations of NaN3 ascorbic acid thiourea

and SOD were 1 mmol L-1 1 mmol L-1 1 mmol L- and 20 U mL-1 respectively

00

02

04

06

08

10

12

14

16

18

Abs

375μM 75μM 1125μM

control ClO- middotOH H2O

2

1O2

middotO2

-

Fig S10The response of this catalytic reaction to various ROSProcedures 50 μL MoS2 (18 μg mL-1) 200 μL Tris-HCl (69 10 mmol L-1) 50 μL TMB (12 mmol L-1) and 200 μL ROS were mixed After the reaction was carried out at room temperature for 30 min The absorbance at 652 nm was recorded

The generation of ROS was performed using documented protocols (Abo et al 2011 J Am Chem Soc 133 10629-10637 Chen et al 2013 J Am Chem Soc 135 11595-11602 Lee et al 2009 Adv Funct Mater 19(12) 1884-1890) Hydroxyl radical (OH) was generated by the Fenton reaction between H2O2 and ferrous sulphate at a molar concentration ratio of 101 Hypochlorite (ClO-) and superoxide anion (O2

-) were obtained from NaOCl and KO2 respectively The singlet oxygen (1O2) was produced by H2O2 with NaClO The absorbance was enhanced greatly at the presence of OH ClO- as well as H2O2

FigS11 Selectivity analysis for glucose detection by monitoring the relative

absorbance Inset of FigS9 were images of colored production for the different

solutions ((a) 5 mmol L-1 fructose (b) 5 mmol L-1 lactose (c) 025 mmol L-1 maltose

(d) 005 mmol L-1 glucose and blank) The error bars represent the standard deviation

of three measurements

00

01

02

03

04

05

06

A-A

0

fructose

5 mmol L-1lactose

5 mmol L-1

maltose

025 mmol L-1

glucose

005 mmol L-1

a b c d

a b c d

e

e

Table S1 Comparison of the apparent Michaelies-Menten constant (Km) and

maximum reaction rate (Vmax) between MoS2 and HRP Km is the Michaelies constant

Vmax is the maximal reaction velocity

Catalyst substance Km (mmol L-1) Vmax (mol L-1s)

MoS2 H2O2 00116 429times10-8

MoS2 TMB 0525 516times10-8

HRP H2O2 109 585times10-8

HRP TMB 0172 418times10-8

Table S2 Results of determination of glucose in serum samples

Glucose meter method a

(mmol L-1)

Proposed method b

(mmol L-1)

RSD

()

Serum 1 500 453plusmn002 c -94

Serum 2 667 631plusmn001 c -54

Serum 3 833 779plusmn001 c -65

a The glucose determination was performed by the conventional enzymatic method at

the First Affiliated Hospital of Fujian Medical Universityb The confidence level was 95c n=3

0 100 200 300 400 500 60000

01

02

03

04

05

06

07

08

Abs

Time s

180 144108072036

MoS2 (ug mL-1)

0

FigS5 The time-dependent absorbance changes at 652 nm in the presence of

different concentrations of MoS2 nanosheets in Tris-HCl buffer (10 mmol L-1 pH 69)

at 30 degC

FigS6 Effect of pH (A) temperature (B) H2O2 concentration (C) and reaction time

(D) on the peroxidase-like activity of MoS2 nanosheets for the TMB oxidation The

experiment was carried out using 18 microg mL-1 MoS2 nanosheets in a reaction volume

of 05 mL in Tris-HCl buffer (10 mmol L-1 pH 69) with 12 mmol L-1 TMB and 004

mmol L-1 H2O2 for 30 min at 30 degC The error bars represent the standard deviation of

three measurements

0 300 600 900 1200 1500 180001

02

03

04

05

06

07

08

09

Abs

Time s

0 1 2 3 4 5 6 7 8 9

00

02

04

06

08

10

12

14

16

18

Abs

pH20 30 40 50 60 70

06

07

08

09

10

11

12

13

14

15

16

17

Abs

Temperature

0 2000 4000 6000 8000 10000

00

05

10

15

20

25

Abs

H2O

2 mmol L-1

A B

C D

FigS7 Steady-state kinetic assay of HRP The velocity (v) of the reaction was

measured using HRP (5 ng L-1) in 05 mL Tris-HCl buffer (10 mmol L-1 pH 69) at

30 (A) The concentration of TMB was 12 mmol L-1 and H2O2 concentration was

varied (B) The concentration of H2O2 was 32 mmol L-1 and TMB concentration was

varied

0000 0002 0004 0006 00080

5

10

15

20

25

30

H2O

2 mol L-1

v10

-8m

olL

-1s-1

01 02 03 04 05

5

10

15

20

25

30

35

v10

-8m

olL

-1s-1

TMBmmol L-1

A B

300 350 400 450 500 550 600 650 700

00

01

02

03

04

05

Abs

Wavelength (nm)

1

2

3

4

5

6

7

FigS8 Absorption spectra of TMB (1) MoS2 nanosheets (7) and a fixed concentration of TMB interacting with different concentrations of MoS2 nanosheets (2-6)

(1) 50 microL TMB (12 mmol L-1) + 50 microL H2O + 200 microL Tris-HCl (pH 69 10 mmol L-1)

+ 200 microL H2O

(2) 50 microL TMB (12 mmol L-1) + 10microL MoS2 (18 microg mL-1) + 40 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(3) 50 microL TMB (12 mmol L-1) + 20 microL MoS2 (18microg mL-1) + 30 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(4) 50 microL TMB (12 mmol L-1) + 30 microL MoS2 (18 microg mL-1) + 20 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(5) 50 microL TMB (12 mmol L-1) + 40 microL MoS2 (18 microg mL-1) + 10 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(6) 50 microL TMB (12 mmol L-1) + 50 microL MoS2 (18 microg mL-1) + 200 microL Tris-HCl (pH69

10 mmol L-1) + 200 microL H2O

(7) 50 microL H2O + 50 microL MoS2 (18 microg mL-1) + 200 microL Tris-HCl (pH6910 mmol L-1) +

200 microL H2O

00

02

04

06

08

10

12

14

Abs

5μL 10μL 15μL 20μL 25μL 30μL

control NaN3

ascorbic acid

thioureaSOD

FigS9 The catalytic reaction at the presence of different radical scavengers

Procedures 50 μL MoS2 (18 μg mL-1) 200 μL Tris-HCl (69 10 mmol L-1) 50 μL

TMB (12 mmol L-1) and different volumes of radical scavenger were mixed The

volume of the mixture was adjusted to 400 μL with water then 200 μL H2O2 (01

mmol L-1) was added After the reaction was carried out at room temperature for 30

min 20 μL H2SO4 (vv20) was added into the above mixture The absorbance at

450 nm was recorded The original concentrations of NaN3 ascorbic acid thiourea

and SOD were 1 mmol L-1 1 mmol L-1 1 mmol L- and 20 U mL-1 respectively

00

02

04

06

08

10

12

14

16

18

Abs

375μM 75μM 1125μM

control ClO- middotOH H2O

2

1O2

middotO2

-

Fig S10The response of this catalytic reaction to various ROSProcedures 50 μL MoS2 (18 μg mL-1) 200 μL Tris-HCl (69 10 mmol L-1) 50 μL TMB (12 mmol L-1) and 200 μL ROS were mixed After the reaction was carried out at room temperature for 30 min The absorbance at 652 nm was recorded

The generation of ROS was performed using documented protocols (Abo et al 2011 J Am Chem Soc 133 10629-10637 Chen et al 2013 J Am Chem Soc 135 11595-11602 Lee et al 2009 Adv Funct Mater 19(12) 1884-1890) Hydroxyl radical (OH) was generated by the Fenton reaction between H2O2 and ferrous sulphate at a molar concentration ratio of 101 Hypochlorite (ClO-) and superoxide anion (O2

-) were obtained from NaOCl and KO2 respectively The singlet oxygen (1O2) was produced by H2O2 with NaClO The absorbance was enhanced greatly at the presence of OH ClO- as well as H2O2

FigS11 Selectivity analysis for glucose detection by monitoring the relative

absorbance Inset of FigS9 were images of colored production for the different

solutions ((a) 5 mmol L-1 fructose (b) 5 mmol L-1 lactose (c) 025 mmol L-1 maltose

(d) 005 mmol L-1 glucose and blank) The error bars represent the standard deviation

of three measurements

00

01

02

03

04

05

06

A-A

0

fructose

5 mmol L-1lactose

5 mmol L-1

maltose

025 mmol L-1

glucose

005 mmol L-1

a b c d

a b c d

e

e

Table S1 Comparison of the apparent Michaelies-Menten constant (Km) and

maximum reaction rate (Vmax) between MoS2 and HRP Km is the Michaelies constant

Vmax is the maximal reaction velocity

Catalyst substance Km (mmol L-1) Vmax (mol L-1s)

MoS2 H2O2 00116 429times10-8

MoS2 TMB 0525 516times10-8

HRP H2O2 109 585times10-8

HRP TMB 0172 418times10-8

Table S2 Results of determination of glucose in serum samples

Glucose meter method a

(mmol L-1)

Proposed method b

(mmol L-1)

RSD

()

Serum 1 500 453plusmn002 c -94

Serum 2 667 631plusmn001 c -54

Serum 3 833 779plusmn001 c -65

a The glucose determination was performed by the conventional enzymatic method at

the First Affiliated Hospital of Fujian Medical Universityb The confidence level was 95c n=3

FigS6 Effect of pH (A) temperature (B) H2O2 concentration (C) and reaction time

(D) on the peroxidase-like activity of MoS2 nanosheets for the TMB oxidation The

experiment was carried out using 18 microg mL-1 MoS2 nanosheets in a reaction volume

of 05 mL in Tris-HCl buffer (10 mmol L-1 pH 69) with 12 mmol L-1 TMB and 004

mmol L-1 H2O2 for 30 min at 30 degC The error bars represent the standard deviation of

three measurements

0 300 600 900 1200 1500 180001

02

03

04

05

06

07

08

09

Abs

Time s

0 1 2 3 4 5 6 7 8 9

00

02

04

06

08

10

12

14

16

18

Abs

pH20 30 40 50 60 70

06

07

08

09

10

11

12

13

14

15

16

17

Abs

Temperature

0 2000 4000 6000 8000 10000

00

05

10

15

20

25

Abs

H2O

2 mmol L-1

A B

C D

FigS7 Steady-state kinetic assay of HRP The velocity (v) of the reaction was

measured using HRP (5 ng L-1) in 05 mL Tris-HCl buffer (10 mmol L-1 pH 69) at

30 (A) The concentration of TMB was 12 mmol L-1 and H2O2 concentration was

varied (B) The concentration of H2O2 was 32 mmol L-1 and TMB concentration was

varied

0000 0002 0004 0006 00080

5

10

15

20

25

30

H2O

2 mol L-1

v10

-8m

olL

-1s-1

01 02 03 04 05

5

10

15

20

25

30

35

v10

-8m

olL

-1s-1

TMBmmol L-1

A B

300 350 400 450 500 550 600 650 700

00

01

02

03

04

05

Abs

Wavelength (nm)

1

2

3

4

5

6

7

FigS8 Absorption spectra of TMB (1) MoS2 nanosheets (7) and a fixed concentration of TMB interacting with different concentrations of MoS2 nanosheets (2-6)

(1) 50 microL TMB (12 mmol L-1) + 50 microL H2O + 200 microL Tris-HCl (pH 69 10 mmol L-1)

+ 200 microL H2O

(2) 50 microL TMB (12 mmol L-1) + 10microL MoS2 (18 microg mL-1) + 40 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(3) 50 microL TMB (12 mmol L-1) + 20 microL MoS2 (18microg mL-1) + 30 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(4) 50 microL TMB (12 mmol L-1) + 30 microL MoS2 (18 microg mL-1) + 20 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(5) 50 microL TMB (12 mmol L-1) + 40 microL MoS2 (18 microg mL-1) + 10 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(6) 50 microL TMB (12 mmol L-1) + 50 microL MoS2 (18 microg mL-1) + 200 microL Tris-HCl (pH69

10 mmol L-1) + 200 microL H2O

(7) 50 microL H2O + 50 microL MoS2 (18 microg mL-1) + 200 microL Tris-HCl (pH6910 mmol L-1) +

200 microL H2O

00

02

04

06

08

10

12

14

Abs

5μL 10μL 15μL 20μL 25μL 30μL

control NaN3

ascorbic acid

thioureaSOD

FigS9 The catalytic reaction at the presence of different radical scavengers

Procedures 50 μL MoS2 (18 μg mL-1) 200 μL Tris-HCl (69 10 mmol L-1) 50 μL

TMB (12 mmol L-1) and different volumes of radical scavenger were mixed The

volume of the mixture was adjusted to 400 μL with water then 200 μL H2O2 (01

mmol L-1) was added After the reaction was carried out at room temperature for 30

min 20 μL H2SO4 (vv20) was added into the above mixture The absorbance at

450 nm was recorded The original concentrations of NaN3 ascorbic acid thiourea

and SOD were 1 mmol L-1 1 mmol L-1 1 mmol L- and 20 U mL-1 respectively

00

02

04

06

08

10

12

14

16

18

Abs

375μM 75μM 1125μM

control ClO- middotOH H2O

2

1O2

middotO2

-

Fig S10The response of this catalytic reaction to various ROSProcedures 50 μL MoS2 (18 μg mL-1) 200 μL Tris-HCl (69 10 mmol L-1) 50 μL TMB (12 mmol L-1) and 200 μL ROS were mixed After the reaction was carried out at room temperature for 30 min The absorbance at 652 nm was recorded

The generation of ROS was performed using documented protocols (Abo et al 2011 J Am Chem Soc 133 10629-10637 Chen et al 2013 J Am Chem Soc 135 11595-11602 Lee et al 2009 Adv Funct Mater 19(12) 1884-1890) Hydroxyl radical (OH) was generated by the Fenton reaction between H2O2 and ferrous sulphate at a molar concentration ratio of 101 Hypochlorite (ClO-) and superoxide anion (O2

-) were obtained from NaOCl and KO2 respectively The singlet oxygen (1O2) was produced by H2O2 with NaClO The absorbance was enhanced greatly at the presence of OH ClO- as well as H2O2

FigS11 Selectivity analysis for glucose detection by monitoring the relative

absorbance Inset of FigS9 were images of colored production for the different

solutions ((a) 5 mmol L-1 fructose (b) 5 mmol L-1 lactose (c) 025 mmol L-1 maltose

(d) 005 mmol L-1 glucose and blank) The error bars represent the standard deviation

of three measurements

00

01

02

03

04

05

06

A-A

0

fructose

5 mmol L-1lactose

5 mmol L-1

maltose

025 mmol L-1

glucose

005 mmol L-1

a b c d

a b c d

e

e

Table S1 Comparison of the apparent Michaelies-Menten constant (Km) and

maximum reaction rate (Vmax) between MoS2 and HRP Km is the Michaelies constant

Vmax is the maximal reaction velocity

Catalyst substance Km (mmol L-1) Vmax (mol L-1s)

MoS2 H2O2 00116 429times10-8

MoS2 TMB 0525 516times10-8

HRP H2O2 109 585times10-8

HRP TMB 0172 418times10-8

Table S2 Results of determination of glucose in serum samples

Glucose meter method a

(mmol L-1)

Proposed method b

(mmol L-1)

RSD

()

Serum 1 500 453plusmn002 c -94

Serum 2 667 631plusmn001 c -54

Serum 3 833 779plusmn001 c -65

a The glucose determination was performed by the conventional enzymatic method at

the First Affiliated Hospital of Fujian Medical Universityb The confidence level was 95c n=3

FigS7 Steady-state kinetic assay of HRP The velocity (v) of the reaction was

measured using HRP (5 ng L-1) in 05 mL Tris-HCl buffer (10 mmol L-1 pH 69) at

30 (A) The concentration of TMB was 12 mmol L-1 and H2O2 concentration was

varied (B) The concentration of H2O2 was 32 mmol L-1 and TMB concentration was

varied

0000 0002 0004 0006 00080

5

10

15

20

25

30

H2O

2 mol L-1

v10

-8m

olL

-1s-1

01 02 03 04 05

5

10

15

20

25

30

35

v10

-8m

olL

-1s-1

TMBmmol L-1

A B

300 350 400 450 500 550 600 650 700

00

01

02

03

04

05

Abs

Wavelength (nm)

1

2

3

4

5

6

7

FigS8 Absorption spectra of TMB (1) MoS2 nanosheets (7) and a fixed concentration of TMB interacting with different concentrations of MoS2 nanosheets (2-6)

(1) 50 microL TMB (12 mmol L-1) + 50 microL H2O + 200 microL Tris-HCl (pH 69 10 mmol L-1)

+ 200 microL H2O

(2) 50 microL TMB (12 mmol L-1) + 10microL MoS2 (18 microg mL-1) + 40 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(3) 50 microL TMB (12 mmol L-1) + 20 microL MoS2 (18microg mL-1) + 30 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(4) 50 microL TMB (12 mmol L-1) + 30 microL MoS2 (18 microg mL-1) + 20 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(5) 50 microL TMB (12 mmol L-1) + 40 microL MoS2 (18 microg mL-1) + 10 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(6) 50 microL TMB (12 mmol L-1) + 50 microL MoS2 (18 microg mL-1) + 200 microL Tris-HCl (pH69

10 mmol L-1) + 200 microL H2O

(7) 50 microL H2O + 50 microL MoS2 (18 microg mL-1) + 200 microL Tris-HCl (pH6910 mmol L-1) +

200 microL H2O

00

02

04

06

08

10

12

14

Abs

5μL 10μL 15μL 20μL 25μL 30μL

control NaN3

ascorbic acid

thioureaSOD

FigS9 The catalytic reaction at the presence of different radical scavengers

Procedures 50 μL MoS2 (18 μg mL-1) 200 μL Tris-HCl (69 10 mmol L-1) 50 μL

TMB (12 mmol L-1) and different volumes of radical scavenger were mixed The

volume of the mixture was adjusted to 400 μL with water then 200 μL H2O2 (01

mmol L-1) was added After the reaction was carried out at room temperature for 30

min 20 μL H2SO4 (vv20) was added into the above mixture The absorbance at

450 nm was recorded The original concentrations of NaN3 ascorbic acid thiourea

and SOD were 1 mmol L-1 1 mmol L-1 1 mmol L- and 20 U mL-1 respectively

00

02

04

06

08

10

12

14

16

18

Abs

375μM 75μM 1125μM

control ClO- middotOH H2O

2

1O2

middotO2

-

Fig S10The response of this catalytic reaction to various ROSProcedures 50 μL MoS2 (18 μg mL-1) 200 μL Tris-HCl (69 10 mmol L-1) 50 μL TMB (12 mmol L-1) and 200 μL ROS were mixed After the reaction was carried out at room temperature for 30 min The absorbance at 652 nm was recorded

The generation of ROS was performed using documented protocols (Abo et al 2011 J Am Chem Soc 133 10629-10637 Chen et al 2013 J Am Chem Soc 135 11595-11602 Lee et al 2009 Adv Funct Mater 19(12) 1884-1890) Hydroxyl radical (OH) was generated by the Fenton reaction between H2O2 and ferrous sulphate at a molar concentration ratio of 101 Hypochlorite (ClO-) and superoxide anion (O2

-) were obtained from NaOCl and KO2 respectively The singlet oxygen (1O2) was produced by H2O2 with NaClO The absorbance was enhanced greatly at the presence of OH ClO- as well as H2O2

FigS11 Selectivity analysis for glucose detection by monitoring the relative

absorbance Inset of FigS9 were images of colored production for the different

solutions ((a) 5 mmol L-1 fructose (b) 5 mmol L-1 lactose (c) 025 mmol L-1 maltose

(d) 005 mmol L-1 glucose and blank) The error bars represent the standard deviation

of three measurements

00

01

02

03

04

05

06

A-A

0

fructose

5 mmol L-1lactose

5 mmol L-1

maltose

025 mmol L-1

glucose

005 mmol L-1

a b c d

a b c d

e

e

Table S1 Comparison of the apparent Michaelies-Menten constant (Km) and

maximum reaction rate (Vmax) between MoS2 and HRP Km is the Michaelies constant

Vmax is the maximal reaction velocity

Catalyst substance Km (mmol L-1) Vmax (mol L-1s)

MoS2 H2O2 00116 429times10-8

MoS2 TMB 0525 516times10-8

HRP H2O2 109 585times10-8

HRP TMB 0172 418times10-8

Table S2 Results of determination of glucose in serum samples

Glucose meter method a

(mmol L-1)

Proposed method b

(mmol L-1)

RSD

()

Serum 1 500 453plusmn002 c -94

Serum 2 667 631plusmn001 c -54

Serum 3 833 779plusmn001 c -65

a The glucose determination was performed by the conventional enzymatic method at

the First Affiliated Hospital of Fujian Medical Universityb The confidence level was 95c n=3

300 350 400 450 500 550 600 650 700

00

01

02

03

04

05

Abs

Wavelength (nm)

1

2

3

4

5

6

7

FigS8 Absorption spectra of TMB (1) MoS2 nanosheets (7) and a fixed concentration of TMB interacting with different concentrations of MoS2 nanosheets (2-6)

(1) 50 microL TMB (12 mmol L-1) + 50 microL H2O + 200 microL Tris-HCl (pH 69 10 mmol L-1)

+ 200 microL H2O

(2) 50 microL TMB (12 mmol L-1) + 10microL MoS2 (18 microg mL-1) + 40 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(3) 50 microL TMB (12 mmol L-1) + 20 microL MoS2 (18microg mL-1) + 30 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(4) 50 microL TMB (12 mmol L-1) + 30 microL MoS2 (18 microg mL-1) + 20 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(5) 50 microL TMB (12 mmol L-1) + 40 microL MoS2 (18 microg mL-1) + 10 microL H2O + 200 microL

Tris-HCl (pH69 10 mmol L-1) + 200 microL H2O

(6) 50 microL TMB (12 mmol L-1) + 50 microL MoS2 (18 microg mL-1) + 200 microL Tris-HCl (pH69

10 mmol L-1) + 200 microL H2O

(7) 50 microL H2O + 50 microL MoS2 (18 microg mL-1) + 200 microL Tris-HCl (pH6910 mmol L-1) +

200 microL H2O

00

02

04

06

08

10

12

14

Abs

5μL 10μL 15μL 20μL 25μL 30μL

control NaN3

ascorbic acid

thioureaSOD

FigS9 The catalytic reaction at the presence of different radical scavengers

Procedures 50 μL MoS2 (18 μg mL-1) 200 μL Tris-HCl (69 10 mmol L-1) 50 μL

TMB (12 mmol L-1) and different volumes of radical scavenger were mixed The

volume of the mixture was adjusted to 400 μL with water then 200 μL H2O2 (01

mmol L-1) was added After the reaction was carried out at room temperature for 30

min 20 μL H2SO4 (vv20) was added into the above mixture The absorbance at

450 nm was recorded The original concentrations of NaN3 ascorbic acid thiourea

and SOD were 1 mmol L-1 1 mmol L-1 1 mmol L- and 20 U mL-1 respectively

00

02

04

06

08

10

12

14

16

18

Abs

375μM 75μM 1125μM

control ClO- middotOH H2O

2

1O2

middotO2

-

Fig S10The response of this catalytic reaction to various ROSProcedures 50 μL MoS2 (18 μg mL-1) 200 μL Tris-HCl (69 10 mmol L-1) 50 μL TMB (12 mmol L-1) and 200 μL ROS were mixed After the reaction was carried out at room temperature for 30 min The absorbance at 652 nm was recorded

The generation of ROS was performed using documented protocols (Abo et al 2011 J Am Chem Soc 133 10629-10637 Chen et al 2013 J Am Chem Soc 135 11595-11602 Lee et al 2009 Adv Funct Mater 19(12) 1884-1890) Hydroxyl radical (OH) was generated by the Fenton reaction between H2O2 and ferrous sulphate at a molar concentration ratio of 101 Hypochlorite (ClO-) and superoxide anion (O2

-) were obtained from NaOCl and KO2 respectively The singlet oxygen (1O2) was produced by H2O2 with NaClO The absorbance was enhanced greatly at the presence of OH ClO- as well as H2O2

FigS11 Selectivity analysis for glucose detection by monitoring the relative

absorbance Inset of FigS9 were images of colored production for the different

solutions ((a) 5 mmol L-1 fructose (b) 5 mmol L-1 lactose (c) 025 mmol L-1 maltose

(d) 005 mmol L-1 glucose and blank) The error bars represent the standard deviation

of three measurements

00

01

02

03

04

05

06

A-A

0

fructose

5 mmol L-1lactose

5 mmol L-1

maltose

025 mmol L-1

glucose

005 mmol L-1

a b c d

a b c d

e

e

Table S1 Comparison of the apparent Michaelies-Menten constant (Km) and

maximum reaction rate (Vmax) between MoS2 and HRP Km is the Michaelies constant

Vmax is the maximal reaction velocity

Catalyst substance Km (mmol L-1) Vmax (mol L-1s)

MoS2 H2O2 00116 429times10-8

MoS2 TMB 0525 516times10-8

HRP H2O2 109 585times10-8

HRP TMB 0172 418times10-8

Table S2 Results of determination of glucose in serum samples

Glucose meter method a

(mmol L-1)

Proposed method b

(mmol L-1)

RSD

()

Serum 1 500 453plusmn002 c -94

Serum 2 667 631plusmn001 c -54

Serum 3 833 779plusmn001 c -65

a The glucose determination was performed by the conventional enzymatic method at

the First Affiliated Hospital of Fujian Medical Universityb The confidence level was 95c n=3

00

02

04

06

08

10

12

14

Abs

5μL 10μL 15μL 20μL 25μL 30μL

control NaN3

ascorbic acid

thioureaSOD

FigS9 The catalytic reaction at the presence of different radical scavengers

Procedures 50 μL MoS2 (18 μg mL-1) 200 μL Tris-HCl (69 10 mmol L-1) 50 μL

TMB (12 mmol L-1) and different volumes of radical scavenger were mixed The

volume of the mixture was adjusted to 400 μL with water then 200 μL H2O2 (01

mmol L-1) was added After the reaction was carried out at room temperature for 30

min 20 μL H2SO4 (vv20) was added into the above mixture The absorbance at

450 nm was recorded The original concentrations of NaN3 ascorbic acid thiourea

and SOD were 1 mmol L-1 1 mmol L-1 1 mmol L- and 20 U mL-1 respectively

00

02

04

06

08

10

12

14

16

18

Abs

375μM 75μM 1125μM

control ClO- middotOH H2O

2

1O2

middotO2

-

Fig S10The response of this catalytic reaction to various ROSProcedures 50 μL MoS2 (18 μg mL-1) 200 μL Tris-HCl (69 10 mmol L-1) 50 μL TMB (12 mmol L-1) and 200 μL ROS were mixed After the reaction was carried out at room temperature for 30 min The absorbance at 652 nm was recorded

The generation of ROS was performed using documented protocols (Abo et al 2011 J Am Chem Soc 133 10629-10637 Chen et al 2013 J Am Chem Soc 135 11595-11602 Lee et al 2009 Adv Funct Mater 19(12) 1884-1890) Hydroxyl radical (OH) was generated by the Fenton reaction between H2O2 and ferrous sulphate at a molar concentration ratio of 101 Hypochlorite (ClO-) and superoxide anion (O2

-) were obtained from NaOCl and KO2 respectively The singlet oxygen (1O2) was produced by H2O2 with NaClO The absorbance was enhanced greatly at the presence of OH ClO- as well as H2O2

FigS11 Selectivity analysis for glucose detection by monitoring the relative

absorbance Inset of FigS9 were images of colored production for the different

solutions ((a) 5 mmol L-1 fructose (b) 5 mmol L-1 lactose (c) 025 mmol L-1 maltose

(d) 005 mmol L-1 glucose and blank) The error bars represent the standard deviation

of three measurements

00

01

02

03

04

05

06

A-A

0

fructose

5 mmol L-1lactose

5 mmol L-1

maltose

025 mmol L-1

glucose

005 mmol L-1

a b c d

a b c d

e

e

Table S1 Comparison of the apparent Michaelies-Menten constant (Km) and

maximum reaction rate (Vmax) between MoS2 and HRP Km is the Michaelies constant

Vmax is the maximal reaction velocity

Catalyst substance Km (mmol L-1) Vmax (mol L-1s)

MoS2 H2O2 00116 429times10-8

MoS2 TMB 0525 516times10-8

HRP H2O2 109 585times10-8

HRP TMB 0172 418times10-8

Table S2 Results of determination of glucose in serum samples

Glucose meter method a

(mmol L-1)

Proposed method b

(mmol L-1)

RSD

()

Serum 1 500 453plusmn002 c -94

Serum 2 667 631plusmn001 c -54

Serum 3 833 779plusmn001 c -65

a The glucose determination was performed by the conventional enzymatic method at

the First Affiliated Hospital of Fujian Medical Universityb The confidence level was 95c n=3

00

02

04

06

08

10

12

14

16

18

Abs

375μM 75μM 1125μM

control ClO- middotOH H2O

2

1O2

middotO2

-

Fig S10The response of this catalytic reaction to various ROSProcedures 50 μL MoS2 (18 μg mL-1) 200 μL Tris-HCl (69 10 mmol L-1) 50 μL TMB (12 mmol L-1) and 200 μL ROS were mixed After the reaction was carried out at room temperature for 30 min The absorbance at 652 nm was recorded

The generation of ROS was performed using documented protocols (Abo et al 2011 J Am Chem Soc 133 10629-10637 Chen et al 2013 J Am Chem Soc 135 11595-11602 Lee et al 2009 Adv Funct Mater 19(12) 1884-1890) Hydroxyl radical (OH) was generated by the Fenton reaction between H2O2 and ferrous sulphate at a molar concentration ratio of 101 Hypochlorite (ClO-) and superoxide anion (O2

-) were obtained from NaOCl and KO2 respectively The singlet oxygen (1O2) was produced by H2O2 with NaClO The absorbance was enhanced greatly at the presence of OH ClO- as well as H2O2

FigS11 Selectivity analysis for glucose detection by monitoring the relative

absorbance Inset of FigS9 were images of colored production for the different

solutions ((a) 5 mmol L-1 fructose (b) 5 mmol L-1 lactose (c) 025 mmol L-1 maltose

(d) 005 mmol L-1 glucose and blank) The error bars represent the standard deviation

of three measurements

00

01

02

03

04

05

06

A-A

0

fructose

5 mmol L-1lactose

5 mmol L-1

maltose

025 mmol L-1

glucose

005 mmol L-1

a b c d

a b c d

e

e

Table S1 Comparison of the apparent Michaelies-Menten constant (Km) and

maximum reaction rate (Vmax) between MoS2 and HRP Km is the Michaelies constant

Vmax is the maximal reaction velocity

Catalyst substance Km (mmol L-1) Vmax (mol L-1s)

MoS2 H2O2 00116 429times10-8

MoS2 TMB 0525 516times10-8

HRP H2O2 109 585times10-8

HRP TMB 0172 418times10-8

Table S2 Results of determination of glucose in serum samples

Glucose meter method a

(mmol L-1)

Proposed method b

(mmol L-1)

RSD

()

Serum 1 500 453plusmn002 c -94

Serum 2 667 631plusmn001 c -54

Serum 3 833 779plusmn001 c -65

a The glucose determination was performed by the conventional enzymatic method at

the First Affiliated Hospital of Fujian Medical Universityb The confidence level was 95c n=3

FigS11 Selectivity analysis for glucose detection by monitoring the relative

absorbance Inset of FigS9 were images of colored production for the different

solutions ((a) 5 mmol L-1 fructose (b) 5 mmol L-1 lactose (c) 025 mmol L-1 maltose

(d) 005 mmol L-1 glucose and blank) The error bars represent the standard deviation

of three measurements

00

01

02

03

04

05

06

A-A

0

fructose

5 mmol L-1lactose

5 mmol L-1

maltose

025 mmol L-1

glucose

005 mmol L-1

a b c d

a b c d

e

e

Table S1 Comparison of the apparent Michaelies-Menten constant (Km) and

maximum reaction rate (Vmax) between MoS2 and HRP Km is the Michaelies constant

Vmax is the maximal reaction velocity

Catalyst substance Km (mmol L-1) Vmax (mol L-1s)

MoS2 H2O2 00116 429times10-8

MoS2 TMB 0525 516times10-8

HRP H2O2 109 585times10-8

HRP TMB 0172 418times10-8

Table S2 Results of determination of glucose in serum samples

Glucose meter method a

(mmol L-1)

Proposed method b

(mmol L-1)

RSD

()

Serum 1 500 453plusmn002 c -94

Serum 2 667 631plusmn001 c -54

Serum 3 833 779plusmn001 c -65

a The glucose determination was performed by the conventional enzymatic method at

the First Affiliated Hospital of Fujian Medical Universityb The confidence level was 95c n=3

Table S1 Comparison of the apparent Michaelies-Menten constant (Km) and

maximum reaction rate (Vmax) between MoS2 and HRP Km is the Michaelies constant

Vmax is the maximal reaction velocity

Catalyst substance Km (mmol L-1) Vmax (mol L-1s)

MoS2 H2O2 00116 429times10-8

MoS2 TMB 0525 516times10-8

HRP H2O2 109 585times10-8

HRP TMB 0172 418times10-8

Table S2 Results of determination of glucose in serum samples

Glucose meter method a

(mmol L-1)

Proposed method b

(mmol L-1)

RSD

()

Serum 1 500 453plusmn002 c -94

Serum 2 667 631plusmn001 c -54

Serum 3 833 779plusmn001 c -65

a The glucose determination was performed by the conventional enzymatic method at

the First Affiliated Hospital of Fujian Medical Universityb The confidence level was 95c n=3

Table S2 Results of determination of glucose in serum samples

Glucose meter method a

(mmol L-1)

Proposed method b

(mmol L-1)

RSD

()

Serum 1 500 453plusmn002 c -94

Serum 2 667 631plusmn001 c -54

Serum 3 833 779plusmn001 c -65

a The glucose determination was performed by the conventional enzymatic method at

the First Affiliated Hospital of Fujian Medical Universityb The confidence level was 95c n=3