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PRC Science Lab Safety Procedures ‐ 1 ‐ 2016‐2017

SCIENCE LABORATORY SAFETY

The following safety procedures and practices apply to all students, faculty and staff working in the Preston Ridge Campus science labs.

For questions or concerns regarding the safety procedures in your laboratory classroom, contact the Science Lab Manager for assistance. Science Lab Manager: Melanie Yates Office: J223 Phone: 972‐377‐1624 Email: [email protected] Lab Safety Training: Laboratory instructors must cover all lab safety practices and waste disposal procedures with their students on the first day of lab. At the beginning of each lab period, instructors should discuss the types of hazardous materials being used for that lab and present the hazards associated with working with these materials. Lab safety procedures are to be enforced at all times. This includes dry labs, open labs, review sessions, quizzes and practical exams. PLEASE NOTE: Any CHEM or BIOL lab students that miss the first day of lab MUST view the Starting with Safety lab safety training DVD that is available in the Science Den, located in Lawler Hall at PRC (Room LH202) before they will be permitted to attend the next lab meeting. The Science Den staff will provide the student with documentation to verify completion that can be returned to the instructor. 1. SAFETY EQUIPMENT: LOCATE THE FOLLOWING IN YOUR LABORATORY AREA: A. Emergency Exit Routes: There are exit signs on or above each door that can be used as an exit route during an emergency. B. Fire Extinguisher: Pull safety pin out, point hose at base of fire, squeeze handle. C. Fire Blanket: Use if clothing should catch fire. Remove the blanket from the container and wrap blanket around the individual. Roll the person on the floor to smother the flames. D. Safety Shower: Should any substance spill on your skin or clothing, stand underneath the shower head at the nearest safety shower station and pull the metal triangle down. E. Eyewash Station: Should any substance be splashed in your eyes, wash them thoroughly at the nearest eye wash station. Bend so that eyes are over the spigots, force eyes wide open then push metal plate or remove plastic caps to start water flow. Rinse eyes for 15 minutes. F. Chemical Splash Goggles and Safety Glasses: To keep a chemical substance from being splashed into your eyes, indirect vent chemical splash goggles must be worn during experiments that involve the use of any potentially hazardous chemicals or materials. Safety glasses must be worn when working around projectiles. Safety glasses, face shields or Visorgog type eyewear are not acceptable alternatives to chemical splash goggles. All BIOL and CHEM students must provide their own chemical splash goggles.


G. First Aid Kit: Minor injuries such as small cuts can be treated effectively in the lab. Open the first aid kit to determine its contents and use as appropriate. Report any injuries to your instructor. Staff and faculty members should report any injuries to the Lab Manager. Injuries or illness should be documented on a Collin College Incident Report and submitted to the Lab Manager. H. Medical Waste Containers: Red plastic medical waste containers are to be used for any glass or sharp object that has come in contact with potentially biohazard material such as bacteria, blood, urine, saliva and/or other human or animal fluids. I. Broken Glass Containers: Plastic containers for any broken glass that has NOT come into contact with biohazard materials or hazardous chemicals described above. J. Bacteriocide Spray: Bottles containing dilute Lysol cleaning solution, used to clean your work areas before and after lab and to clean up most spills. K. Chemical Waste Containers: Chemical wastes should never be poured down a drain or discarded in a trash can. During the semester, specific chemical waste containers will be available to discard chemical wastes. Consult with your instructor or the Lab Manager if you are unsure about a disposal procedure. Chemical waste containers must be closed at all times unless waste is actively being added to the container. L. Emergency Telephone: The phone in the classroom or prep area may be used to call for 911 emergency services and Campus Police. Campus Police – dial 5555. M. Safety Data Sheets: SDS/MSDS Binders are available in each laboratory classroom and prep area for all chemicals in use during your lab course. The SDS Binder for this room should be located on or near the instructor’s bench. Locate the binder for your work area and familiarize yourself with the information provided by the Safety Data Sheet. 2. DO NOT eat, drink, smoke/use tobacco products, store food, or apply cosmetics in the laboratory. Faculty and staff are permitted to have drinks in CLOSED containers at PREP AREA computer workstations only. Food and drink are never permitted in any of the laboratory classrooms. 3. WASH your hands with soap and warm water before leaving the lab and/or after removing contaminated gloves. Do not wear personal protective equipment (gloves, lab coats, etc.) out of the laboratory area unless you are transporting hazardous materials or wastes. 4. CLEAN and disinfect work surfaces at the beginning and end of every lab period. Keep extra books, bags and clothing in designated places so your work area is uncluttered. 5. DRESS: The following statements regarding dress apply to all faculty, staff, and students unless otherwise stated below.

Restrain long hair, loose clothing and dangling jewelry. Sturdy and enclosed closed‐toe shoes are the ONLY appropriate footwear for a laboratory at any time. DO

NOT wear sandals, flip‐flops or other open‐toed footwear. Those wearing inappropriate footwear will not be permitted into the laboratory. Shoes should cover the entire foot, including the top of the foot, toes and heel. Toe shoes, barefoot style sport shoes, slippers and other ‘soft’ shoes made of mostly absorbent fabrics or shoes that expose any part of the foot are not permitted.

Contact lenses are an added safety risk and should not be worn while performing lab exercises. Take the necessary precautions when wearing contact lenses in the lab.

Indirect vent chemical splash goggles are required to be worn by all staff, students and instructors during the use of hazardous chemicals, biohazard materials and/or dissections. Those without appropriate eye protection will not be permitted into the laboratory and will be asked to leave. Goggles must fit appropriately over eyeglasses.


Clothing that leaves any part of the legs or ankles exposed is never recommended in the laboratory setting and may be restricted by your instructor or supervisor. Shorts, tank tops, miniskirts, and sleeveless shirts should not be worn when working with hazardous chemicals/wastes, biological agents or when performing dissections.

All Chemistry lab students, faculty and staff are required to wear a lab coat or laboratory apron when working with hazardous chemicals or wastes of any kind.

All students, faculty and staff are required to wear gloves when working with hazardous chemicals, biohazards/potentially pathogenic materials, and/or when performing dissections.

All Microbiology students (2420, 2421), faculty and staff must wear a disposable lab coat when working with biological materials or hazardous chemicals/wastes. These lab coats are to remain in the microbiology lab for the duration of the semester and will be sterilized and disposed of at the end of the term.

All Biology students (1406/1408/1407/1409/2401/2402), faculty and staff must wear a disposable lab coat OR apron when working with hazardous materials. This includes work with hazardous chemicals, when performing dissections, when working with bacteria and when handing chemical or biohazard wastes.

Students must provide their own lab coat or apron, goggles and gloves. Faculty and staff should contact the Lab Manager if they need a lab coat, apron or goggles.

6. MONITOR RISK: Inform the instructor if you are pregnant, taking immunosuppressive medicines or have any medical condition that might require special precautions in the lab, such as medications that would influence your response or reflex time. Under no circumstance should you attend a lab session or work in the laboratory setting while under the influence of any chemical substance. Situations in which drug or alcohol use is suspected in the laboratory, or elsewhere on campus, will be immediately referred to the Collin Police Department. Students that are unable to wear chemical splash goggles or closed toe shoes or are unable to adhere to any safety related requirements due to medical reasons should contact the ACCESS Office for appropriate accommodations to be made. Otherwise, all students are expected to adhere to all safety requirements. 7. HEATING APPARATUS: Use chemical splash goggles for all experiments in which solutions, chemicals or glassware are heated. NEVER leave any heat source unattended. Point the mouth of a test tube away from yourself and other people when heating the tube. Handle hot glassware with tongs, safety gloves and other appropriate aids – NEVER BARE HANDS. Turn OFF and UNPLUG non‐essential laboratory equipment when not in use. 8. AVOID SPILLS: Place liquids toward the center of the bench, away from the edges. Disinfect any equipment that comes into contact with live material or body fluids, and clean up any spills as soon as they occur. Notify your instructor or the Lab Manager of any chemical spills. 9. LABELS: READ labels carefully before removing substances from their containers. PROPERLY label glassware before use. Containers of unknown (unlabeled) chemicals should be brought to the attention of your instructor or a member of the lab staff. 10. MOUTH PIPETTING is strictly prohibited; use mechanical pipetting devices only. 11. DISCARD used chemicals and biohazardous materials into appropriately labeled containers – do not dispose of them down the sink or in trash cans. Students may not remove chemicals or biologicals from the laboratory classroom under any circumstance. Hazardous materials may only be taken out of the lab by authorized laboratory personnel or faculty with the approval of the Lab Manager. 12. BROKEN GLASS: Do not handle broken glassware with bare hands. Dispose of all cracked or broken glassware in a puncture‐resistant container – NOT the regular trash. Discuss with instructor what was broken and where to discard it. Discard broken glassware contaminated with hazardous chemicals in an appropriately labeled container.


See your instructor or a member of the lab staff if a hazardous waste/broken glass container is needed. Lubricate glass pieces before inserting into corks, stoppers, etc. To prevent glass breakage, insert glass with a twisting motion. 13. INCIDENT REPORT: Report all spills, accidents, strange occurrences, or other safety incidents PROMPTLY to the instructor or a member of the lab staff.

Lab staff will forward all incident reports to the Lab Manager as well as promptly report any identified or potential safety hazards or violations.

Lab staff should notify the Lab Manager when emergency personnel have been called to their area.

If you are injured or become ill in the lab, do not leave the lab area without first notifying your instructor, your lab partner, or a staff member.

14. PROFESSIONAL CONDUCT is required to avoid creating dangerous situations. Do NOT perform unauthorized experiments, touch or handle laboratory equipment without your instructor’s approval, work in the lab without faculty supervision, or carry out practical jokes or pranks. If you have any questions concerning the safety of a procedure, consult with your instructor or the Lab Manager before carrying out any portion of your experiment or laboratory task. Faculty and Staff: Do not work with hazardous chemicals while completely alone in the laboratory. Perform these and other potentially hazardous duties while in the presence of another staff or faculty member. Students: You must have the permission of your instructor and be in the presence of an informed faculty member to work in the lab or attend another lab section outside of your regularly scheduled lab time. Notify your lab partner and your lab instructor immediately in the event of an accident, spill, injury or other hazardous condition. 15. LABORATORY DOORS: All laboratory doors must be closed in accordance with local fire safety codes. If you leave, inform your instructor who can then designate someone to open the door at your knock when you return. Do not prop the laboratory doors open. 16. BIOSAFETY: Wear gloves, lab coat, and goggles when handling biohazard materials. Do not, under any circumstances, remove any biohazard materials from the laboratory classroom. Dispose of all biohazard wastes in the appropriately labeled can, bucket or bottle. Practice good aseptic technique and wash your hands thoroughly. Label everything! Unlabeled tubes and petri dishes may pose a risk to other students and laboratory personnel and must be disposed of as biological waste when discovered. 17. CHEMICAL SAFETY: Review the Chemical Hygiene Plan prior to using any chemicals in the laboratory. The CHP is available in the front section of the SDS binder at the Instructor’s Desk and in each laboratory prep area.

Consult the Safety Data Sheet (SDS) prior to using any hazardous chemical. SDS are available in a binder at or near the instructor’s bench and in each laboratory prep area.

Treat every chemical as if it were hazardous.

Instructors will provide chemical specific safety information prior to the start of each laboratory exercise.

If you are ever unsure about the hazards of a particular chemical in use, consult your instructor or the Lab Manager before handling the chemical.

Pour more concentrated solutions into less concentrated solutions to avoid violent reactions (i.e., always add acid to water; not water to acid).

Adhere to chemical waste disposal procedures.

Use fume hoods whenever possible.

Never taste chemicals. Never directly smell a chemical. To safely smell a chemical, wave the vapors towards the nose.

Tightly secure bottle or container lids when you are finished using them. Do not return leftover chemicals to the original container.

Do not use open flames while using ether.


18. PERSONAL HYGEINE: Remain aware of your surroundings and actions when working with chemicals and biohazard materials.

Always wash your hands before touching your personal belongings during the use of chemicals and biohazard substances.

Do not touch your clothing, hair, face, mouth, nose or eyes with gloved or unwashed hands.

Do not put writing utensils or laboratory equipment in your mouth while working in the lab. 19. EMERGENCY RESPONSE: Always notify a member of the lab staff in the event of an injury or illness during your lab. The lab staff is there to assist in these situations. Members of the lab staff or faculty should contact emergency personnel in the event of a fire, serious injury or illness in the laboratory areas. Collin College Police ext. 5555 Frisco Emergency Services 911 Fire Emergency Procedure: 1) Confine the fire or emergency; 2) Alert personnel in the immediate vicinity of the crises 3) Summon all necessary aid 4) Evacuate the building if necessary Chemical Injury to the Eye: 1) GET TO ANY WATER SOURCE IMMEDIATELY. This may be the eyewash or any sink near the accident site. 2) WASH THE EYE THOROUGHLY WITH PLAIN WATER for no less than 15 minutes. The eye is a moist environment that readily absorbs many chemicals into tissues below the outer surface, so a 15 minute wash is necessary to leach out the chemicals that have penetrated the eye. Alkaline materials, such as ammonia, are far more serious in the eye than are strong acid solutions because acids will precipitate a protein barrier and prevent further penetration of the eye, whereas a strong base will not (that means a base will penetrate deeper and cause more tissues to be damaged). Burns from Fire or Chemicals: 1) A person with burning clothes should be prevented from running and the flames should be SMOTHERED WITH FIRE BLANKET. 2) Thermal burns can be best treated, IF THE SKIN IS NOT BROKEN, by immersing the burned areas in cool or cold water. 3) Chemical burns must be treated first with thorough washing of the burned areas with water. SPEED in washing the burn site is critical and the burn must be flushed with water. Cuts and Abrasions: 1) IMMEDIATELY cleanse wound and surrounding skin with soap and warm water, wiping AWAY from the wound. 2) Hold a sterile pad over the wound until bleeding stops. The pad should be held firmly over the open wound. 20. ELECTRICAL EQUIPMENT: Some experiments will require electric circuits to be connected to a power supply (instead of a battery). In these cases, under NO circumstances are electric circuit connections to be touched while the power supply is on. If a connection needs to be adjusted, TURN THE POWER SUPPLY OFF BEFORE MAKING ADJUSTMENTS! Unplug any equipment that is not in use. Never leave equipment running unattended.


Texas Hazard Communication Act The Texas Hazard Communication Act (Right‐to‐Know Act), Chapter 502 of the Texas Health and Safety Code (revised 1993) requires Collin County Community College to: Provide students, employees, fire departments, the Texas Department of Health, and other persons with specific information on the hazards of chemical in use. As required by this law, Collin County Community College must provide the student with certain information and training. A brief summary of the law follows. 1. Students who may be exposed to hazardous chemicals or biologicals shall be informed of the exposure and shall have ready access to the most current Safety Data Sheets (SDS), which detail physical and health hazards and other pertinent information. 2. Students shall receive training by the college on the hazards of the chemicals and biologicals to which they may be exposed and on the measures they can take to protect themselves from those hazards. 3. Students shall not be required to work with hazardous chemicals from unlabeled containers, except portable containers for immediate use, the contents of which are known by the user. 4. Students may file complaints with the Texas Department of Health, and may not be discriminated against in any manner for the exercise of any rights provided by this Act. Hazardous Chemicals Definition: Hazardous chemicals are any products or materials that present any physical or health hazards when used, unless they are exempted under the law. Some examples of more commonly used hazardous chemicals are fuels, cleaning products, solvents, many types of oils, compressed gases, many types of paints, pesticides, herbicides, refrigerants, laboratory chemicals, cement, welding rods, etc. In March of 2012, the Occupational Safety and Health Administration (OSHA) formally adopted the Globally Harmonized System of Classification and Labeling of Chemicals (GHS) and the Hazard Communication Standard (HCS) now aligns with the GHS. Access the HCS/HazCom 2012 Final Rule here. http://www.osha.gov/dsg/hazcom/ghs‐final‐rule.html.

Major Changes to the Hazard Communication Standard Hazard classification: Provides specific criteria for classification of health and physical hazards, as well as classification of mixtures. Labels: Chemical manufacturers and importers will be required to provide a label that includes a harmonized signal word, pictogram, and hazard statement for each hazard class and category. Precautionary statements must also be provided. Safety Data Sheets: Now have a specified 16‐section format. Information and Training: Employers are required to train workers by December 1, 2013 on the new labels elements and safety data sheets format to facilitate recognition and understanding. Faculty and staff hired after December 1, 2013 must also complete training prior to working in the labs. We are continuously in the process of updating our classroom MSDS binders to the newly reformatted Safety Data Sheets now available from our chemical manufacturers. During this transition, you may find both MSDS and SDS in the binders in your classrooms.


Safety Data Sheets (SDS) A Safety Data Sheet (SDS), formerly known as a Material Safety Data Sheet (MSDS) is prepared by the chemical manufacturer in accordance with OSHA's 2012 Hazard Communication Standard and indicates the hazard and safe handling information for a chemical. All SDS for all chemicals used in the laboratory are located in a 3‐ring binder on or near the instructor’s desk. As of June 1, 2015, the HCS will require new SDSs to be in a uniform format, and include the section numbers, the headings, and associated information under the headings below:

Section 1, Identification includes product identifier; manufacturer or distributor name, address, phone number; emergency phone number; recommended use; restrictions on use.

Section 2, Hazard(s) identification includes all hazards regarding the chemical; required label elements.

Section 3, Composition/information on ingredients includes information on chemical ingredients; trade secret claims.

Section 4, First‐aid measures includes important symptoms/ effects, acute, delayed; required treatment.

Section 5, Fire‐fighting measures lists suitable extinguishing techniques, equipment; chemical hazards from fire.

Section 6, Accidental release measures lists emergency procedures; protective equipment; proper methods of containment and cleanup.

Section 7, Handling and storage lists precautions for safe handling and storage, including incompatibilities.

Section 8, Exposure controls/personal protection lists OSHA's Permissible Exposure Limits (PELs); Threshold Limit Values (TLVs); appropriate engineering controls; personal protective equipment (PPE).

Section 9, Physical and chemical properties lists the chemical's characteristics.

Section 10, Stability and reactivity lists chemical stability and possibility of hazardous reactions.

Section 11, Toxicological information includes routes of exposure; related symptoms, acute and chronic effects; numerical measures of toxicity.

Section 12, Ecological information*

Section 13, Disposal considerations*

Section 14, Transport information*

Section 15, Regulatory information*

Section 16, Other information, includes the date of preparation or last revision.

*Note: Since other Agencies regulate this information, OSHA will not be enforcing Sections 12‐15.

Employers must ensure that SDSs are readily accessible to employees.


GHS Labels

Sample Manufacturer Label

GHS Pictograms


Laboratory Waste Disposal As a Conditionally Exempt Small Quantity Generator of hazardous wastes, the PRC Science Labs must complete a waste stream determination for any waste streams generated by the labs. Students are not permitted to dispose of laboratory wastes (hazardous, non‐hazardous, biological, dissection, universal) without first consulting with their instructor or a member of the lab staff. All laboratory wastes must be profiled prior to disposal so please consult with the Lab Manager or a member of the lab staff prior to disposing of any laboratory wastes. Hazardous Waste Definition: A substance which exhibits one of the four hazardous characteristics (corrosivity, ignitability, reactivity, toxicity) or is specifically listed as hazardous waste by the EPA. Because of potential risk of harmful exposure, hazardous waste is regulated by both federal and state law. Hazardous wastes are strictly prohibited from drain disposal. All hazardous wastes are to be collected in the appropriately labeled container provided by the lab staff. This container must be kept CLOSED at all times when not being filled. Examples include acidic or basic solutions, heavy metals, dissection preservative and organic solvents. Non‐Hazardous Waste Definition: Chemical waste that does not exhibit the characteristics of corrosivity, ignitability, toxicity or reactivity OR appear on the EPA published F, K, P and/or U lists. Although non‐hazardous, these chemical wastes must not be indiscriminately placed in the regular trash or disposed of down the drain in order to comply with local regulations. All non‐hazardous wastes are to be collected in the appropriately labeled container provided by the lab staff. This container must be kept CLOSED at all times when not being filled. Examples include Benedict’s Solution, glucose, sucrose, simulated blood and wastes containing zinc, copper and aluminum. Universal Waste Definition: Universal waste is hazardous waste that is permitted for recycling or special handling. Spent batteries, mercury containing devices and lamps are examples that you may encounter in the labs. Universal wastes are collected in appropriately labeled containers provided by the lab staff. Biohazard/Biological Waste Definition: Any waste that is potentially biohazardous, infectious, or pathological. Biological waste includes animal blood, blood products, dissection waste from preserved or fresh specimens, contaminated petri dishes, and tubed media. All waste that is contaminated with bacteria or an otherwise potentially infectious substance is heavily regulated by the State of Texas. All contaminated waste of this nature must be discarded in the properly labeled containers in your laboratory classroom. Biohazard waste is never to be disposed of in a laboratory drain or trash can. The following tables outline the basic discard procedures for several types of chemical and biohazard waste that will be generated in your science labs. Consult with your instructor immediately if you are ever unsure of where or how to dispose or discard your laboratory wastes.


Chemical Waste Table

Hazardous Waste

Containers In Fume

Hood or on Cart

1

Non‐Hazardous Waste

Containers In Fume

Hood or on Cart

2

Red Sharps Container

3

Broken Glass Bucket

4

Blue Recycle Trash Cans

(paper, plastic and aluminum cans)

5

Black Trash Cans

(Regular Trash)

6

Notify Your Instructor

for Procedure

7

Pipet Discard Tray In Fume Hood or on

Cart8

Ignitable chemical waste (flash point <60° C) Example: hexane

Toxic chemical waste (heavy metals and some solvents) Example: silver

Reactive chemical waste Example: sulfides

Corrosive chemical waste (pH ≤5.0 or ≥10.5) Example: hydrochloric acid, sodium hydroxide

F, K, P, U Listed wastes Example: methylene chloride

Non‐Hazardous wastes Example: aluminum, zinc and ammonium hydroxide solutions

Used weigh boats, weighing papers, plastic scoops (free of chemicals)

Kim Wipes, used to clean cuvettes

Recyclables: paper, plastic, aluminum cans

Paper towels used for routine cleaning

Gloves worn for routine protection

Broken glassware (no chemical residue)

Broken glassware (contains hazardous chemicals)

Broken glassware (contaminated with blood)

Broken flasks, Buchner funnels, adapter tubes, condensers or distilling apparatus

Hypodermic Needles, Syringes Used pipettes: Serological, transfer, Pasteur

Broken alcohol thermometers

Broken mercury thermometers

1) Hazardous Waste: Hazardous waste is handled and disposed of in accordance with the Resource Conservation and Recovery Act (RCRA). 2) Non‐Hazardous Waste: Non‐Hazardous waste is handled and disposed of in accordance with the City of Frisco’s Code of Ordinances and with permission from the North Texas Municipal Water District. Disposal methods vary according to actual constituents of the waste. 3) Red Sharps Containers: Regulated medical waste is disposed of through a contracted vendor. NO chemical waste. 4) Broken Glass Bucket: Uncontaminated broken glass is disposed of as solid waste in a puncture resistant container. NO chemical waste. 5) Blue Trash Cans: The blue cans are for recyclables only. Clean scrap paper, plastic, aluminum cans, etc. NO chemical waste. NO broken glass. 6) Black Trash Cans: Items thrown into the regular black trash cans are for uncontaminated waste only. This waste goes to the dumpsters. 7) Notify the lab staff in the event of broken controlled glassware or broken glass involving a hazardous chemical. 8) Pipettes are washed and reused when practical and safe to do so.


Biological Waste Table

Step Can

w/ Biohazard

Bag1

Red Sharps

Container2

Pipette Tray on Cart

3

White Buckets on Back Counter

4

(micro lab only)

Regular Trash

5

Broken Glass Bucket6

Labeled Rack Under Fume

Hood7

(micro lab only)

Animal Waste Can

w/ Biohazard Liner

8 Contaminated gloves and paper

Contaminated plastic Petri dishes, specimen cups (NO urine*)

Used lens paper and bibulous paper

Micro Lab: Gloves worn for routine protection or staining

Used slides and cover slips

Micropipette tips

Contaminated Pasteur and transfer pipettes

Contaminated swabs, spreaders, plastic loops

Contaminated glass serological pipettes

Contaminated plastic serological pipettes

Glass culture tubes, labels removed

Uncontaminated waste: swab wrappers, foil pouches, lens paper, paper towels, etc.

Paper towels used after hand washing & drying

Paper towels used for routine cleaning of bench before and after lab period

Paper towels used to wipe up biohazard spills

Uncontaminated broken glass

Plated media – not inoculated

Tubed media – not inoculated

Tubes of biohazard and hazardous waste

Dissection Waste (carcasses, body parts; fresh and preserved)

Gloves and paper towels used for dissections

1) Biohazard bags (from step cans): bags are sterilized on‐site in accordance with the provisions of 25 TAC §1.136 and disposed of as solid waste. 2) Red Sharps Container: regulated medical waste is disposed of through a contracted vendor. 3) Pipette Tray (for GLASS serological pipettes only): glass pipettes are disinfected and reused. 4) White Buckets (for tubes): tubes and caps are autoclaved and reused. Sterilized liquid media is disposed of via sanitary sewer. Sanitized solid media is collected in a container, labeled in accordance with the provisions of 25 TAC §1.136 and disposed of as solid waste. 5) Regular Trash: items thrown into the regular trash cans are for uncontaminated waste only. This waste goes to the dumpsters. 6) Broken Glass Bucket: uncontaminated broken glass is disposed of in a puncture resistant container. 7) Biohazard + Hazardous Waste: Tubes are first disinfected to remove biohazard. Remaining waste is treated and disposed of as Hazardous Waste. 8) Animal wastes (carcasses, tissues, bones, skin) are collected for incineration through a contracted vendor.


What Cannot Go Down the Drain?

Hazardous wastes on P, U, K and F lists. http://epa.gov/osw/hazard/wastetypes/listed.htm

Ignitable wastes: Ignitable wastes can create fires under certain conditions, are spontaneously combustible, or have a flash point less than 60 °C (140 °F). Examples include waste oils and used solvents.

Corrosive wastes: Corrosive wastes are acids or bases (pH less than or equal to 2, or greater than or equal to 12.5) that are capable of corroding

metal containers, such as storage tanks, drums, and barrels. Hydrochloric acid and sodium hydroxide are examples. Acidic and basic solutions can be neutralized and disposed of down the drain with copious amounts of water. This waste, along with your neutralization procedure, initial/final pH and volumes would be recorded on your log for sink disposal.

Reactive wastes: Reactive wastes are unstable under "normal" conditions. They can cause explosions, toxic fumes, gases, or vapors when heated,

compressed, or mixed with water. Examples include sodium, lithium‐sulfur batteries and explosives.

Toxic wastes: Toxic wastes are harmful or fatal when ingested or absorbed (e.g., containing mercury, lead, etc.). When toxic wastes are land disposed, contaminated liquid may leach from the waste and pollute ground water.

Any solutions or compounds containing any of the following:

Arsenic Cadmium Chromium Copper Cyanide Lead Mercury Nickel Selenium Silver Silver (free ion) Zinc Phenols Aluminum Ammonia nitrogen Aldrain Aluminum Carbaryl Chlordane Chlorpyrifos

DDT Demeton Dieldron Endosulfan Guthion Heptachlor Hexachlorocyclohexane Malathion Methoxychlor Mirex PCB (total) Parathion Phenanthrene Pentachlorophenol Selenium Silver (free ion) Toxaphene Tributyltin 2‐4‐5‐Tricholorophenol

Any wastewater having a pH less than 5.0, greater than 10.5.

Any liquid or vapor having a temperature higher than 150 degrees (65 degrees); or exhibiting heat in amounts which will inhibit biological activity in a treatment plant causing an interference; or, in any case, exhibiting heat in such quantities that the temperature at the treatment plant exceeds 40 degrees Celsius (104 degrees Fahrenheit).

Any free or emulsified fats, waxes, greases or oils containing substances which may solidify or become viscous at temperatures between 32 and 150

degrees Fahrenheit (0 and 65 degrees Celsius).

Petroleum oil, nonbiodegradable cutting oil, or products of mineral oil origin.

Any material or liquid with the potential to create an obstruction within the sanitary sewer.

Any noxious or malodorous liquids.

Kerosene, naphtha, benzene, toluene, xylene, ethers, perchlorates, bromates, carbides, hydrides, sulfides or any other substances which are a fire or other hazard to the system.

Any substance which creates a fire or explosion hazard in the POTW, including, but not limited to, wastestreams with a closed cup flashpoint of less

than 140 degrees Fahrenheit or 60 degrees Centigrade using the test methods specified in 40 CFR 261.21.

Untreated biohazard waste, untreated animal blood. Treated biohazard waste contaminated with hazardous chemical waste.

City of Frisco Local Ordinance – Chapter 94 Utilities Article II. Public Sewers and Industrial Wastes Discharge PART II ‐ CODE OF ORDINANCES \ CHAPTER 94 UTILITIES \ ARTICLE II. PUBLIC SEWERS AND INDUSTRIAL WASTES DISCHARGE \ Sec. 94‐20 Prohibited discharges

RCRA Subtitle C – Hazardous Waste Regulations http://www.epa.gov/osw/laws‐regs/regs‐haz.htm

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