SCENAR-therapy SCENAR-therapy as a universal method for as a universal method for managing oxidative stressmanaging oxidative stress

Emergency Care DepartmentEmergency Care Department,, Rostov State Medical University, Rostov State Medical University, Rostov-on-DonRostov-on-Don, ,

RITM OKB ZAORITM OKB ZAO, , TaganrogTaganrog, , RussiaRussia

L.V. Klimova, A.V. Tarakanov, A.J. CherchagoL.V. Klimova, A.V. Tarakanov, A.J. Cherchago

Oxidative stressOxidative stress

Increases the Increases the deleterious effectdeleterious effect of of::

xenobioticxenobioticss, hypoxia, , hypoxia, ischemiaischemia andand reperfusion, reperfusion, inflammationinflammation, , bacterialbacterial and and virus infectionsvirus infections, , alcoholalcohol, hyperglycemia, hyperglycemia (diabetes), (diabetes), ......

Greatly influences onGreatly influences on::

reproductionreproduction, , ontogenesis and cell ontogenesis and cell proliferationproliferation, , carcinogenesis, carcinogenesis, atherogenesis, ageing processatherogenesis, ageing processeses, , ……

Oxidative stressOxidative stress

is prooxidant – antioxidant prooxidant – antioxidant disbalancedisbalance

with prooxidants prevailing and antioxidant systems astrainwith prooxidants prevailing and antioxidant systems astrain

What is What is prooxidantprooxidant??

OxygenOxygen

MakesMakes 65% 65% of the human body weightof the human body weight

We breath inWe breath in 400400 litres of litres of ОО22 daily daily

““The amount of free oxygen is the The amount of free oxygen is the chemical measure for the living chemical measure for the living matter”matter”

V.I. VernadskyV.I. Vernadsky

Physiological norm Physiological norm

95-98%95-98% of the of the used used ОО22 goes on producing goes on producing

energy and providing oxidative catabolismenergy and providing oxidative catabolism

2-5%2-5% turns into turns into prooxidantsprooxidants thatthat in physiological conditions play the key role in the in physiological conditions play the key role in the

cell metabolism, maintain the cell metabolism, maintain the homeostasishomeostasis of the of the body internal environmentbody internal environment and its adaptive and its adaptive

reactions.reactions.

Prooxidants are:activated oxygen metabolites, active oxygen forms, free radicals of this

etiology

In pathology the amount of In pathology the amount of prooxidants increases and free prooxidants increases and free

radical oxidation activation radical oxidation activation processes of all boimolecules, in processes of all boimolecules, in

particular lipids of biological particular lipids of biological membranes, go out of control. membranes, go out of control.

Structure of the cell membraneStructure of the cell membrane

Lipid peroxidation productsLipid peroxidation products

produce toxic effect due to produce toxic effect due to biopolymerbiopolymer linking, linking, mitochondrial swellingmitochondrial swelling and division and division of of oxidative phosphorylationoxidative phosphorylation, as well as , as well as

inactivation of enzymes that are involved in inactivation of enzymes that are involved in breathing and glycolysis. They oxidate breathing and glycolysis. They oxidate

proteins, damage DNA, can slow down and proteins, damage DNA, can slow down and even stop division and growth of cells, even stop division and growth of cells,

destabilize cell membranes. destabilize cell membranes.

ANTIOXIDANTis any substance which, when present at low concentrations relative to the

substrate being oxidized, will inhibit or retard that oxidation

Halliwell B., Gutteridge J.M.C. (1989)

AntioxidantsAntioxidantsDiffer in chemical nature, Differ in chemical nature, dissolubilitydissolubility, , molecular massmolecular mass, ,

cell specificity, action mechanism and principle.cell specificity, action mechanism and principle.

Antioxidants are enzymes, Antioxidants are enzymes, polyphenolpolyphenols, s, flavonoidsflavonoids, , hormoneshormones, vitamines, selenic and , vitamines, selenic and thioic thioic compounds, compounds, carnosine, ubiquinones, etc. carnosine, ubiquinones, etc.

They are regenerated in the body They are regenerated in the body non-stop, as well as come with non-stop, as well as come with food or antioxidative drugs. food or antioxidative drugs.

AntioxidantsAntioxidants

can be either can be either directdirect or or indirectindirect in their in their effecteffect

Direct antioxidantsDirect antioxidants

act act in vivoin vivo,, as well as as well as in vitroin vitro. Direct antioxidants . Direct antioxidants are most endogenic and are most endogenic and syntheticsynthetic antioxidants. antioxidants.

Commonly antioxidants are functionally Commonly antioxidants are functionally connected with each other, can be in connected with each other, can be in synergetic or antagonistic interaction, synergetic or antagonistic interaction, have a dose-dependent effect, and thus have a dose-dependent effect, and thus the drug-management of the oxidative the drug-management of the oxidative stress in any pathology can be stress in any pathology can be problematic. problematic.

Indirect antioxidants Indirect antioxidants

act onlyact only in vivoin vivo, , i.e. in the alive systems by i.e. in the alive systems by stimulating the stimulating the synthesissynthesis of endogenic of endogenic

antioxidants, stabilization of cell antioxidants, stabilization of cell membranes and normalization of the membranes and normalization of the

metabolic processes. metabolic processes.

Such an effect can be when using not only Such an effect can be when using not only chemical substances, but a number of chemical substances, but a number of

non-drug therapies (for example, non-drug therapies (for example, SCENAR-therapySCENAR-therapy). ).

Since late 90th the influence of Since late 90th the influence of SCENAR-therapy SCENAR-therapy on the processes associated with oxidative on the processes associated with oxidative stress in patients with various acute and stress in patients with various acute and chronic pathologies is in the focus of the chronic pathologies is in the focus of the researches.researches.

Most biochemical researches were Most biochemical researches were performed in performed in biochemical laboratory (Research Institute of biochemical laboratory (Research Institute of Biology, Southern Federal University) by Biology, Southern Federal University) by researches from SFU and Rostov State Medical researches from SFU and Rostov State Medical University, as well as doctors from several University, as well as doctors from several medical institutions of Rostov-on-Don medical institutions of Rostov-on-Don performed laboratory researches. performed laboratory researches.

Some research activities were performed in the Some research activities were performed in the Perm Medical Academy. Perm Medical Academy.

Pilot Pilot Scientific Research Scientific Research

Acute Acute experimental pancreatitisexperimental pancreatitis (2006)(2006)

Experimental animalExperimental animalss– – lab rats with various stages lab rats with various stages of acute of acute experimental pancreatitisexperimental pancreatitis

Research organisationResearch organisation: : Rostov State Medical Rostov State Medical UniversityUniversity

To administer To administer SCENAR-therapySCENAR-therapy researches have researches have constructed special cell in the body of the lab constructed special cell in the body of the lab animals which will enable stimulation with animals which will enable stimulation with SCENAR 97.4 of the inner side of the rat’s paw SCENAR 97.4 of the inner side of the rat’s paw setting the stimulation energy that doesn’t involve setting the stimulation energy that doesn’t involve painful sensations at a 60Hz frequency. painful sensations at a 60Hz frequency.

Treatment courseTreatment course: : 10 sessions, 15 minutes each, 10 sessions, 15 minutes each, daily, beginning with the first AEPdaily, beginning with the first AEP..

Blood plasmaBlood plasma and and erythrocyteerythrocytes were analyzed s were analyzed

HBO-induced oxidative stress (2009)HBO-induced oxidative stress (2009)

Experimental animalsExperimental animals - - lab white rats that were lab white rats that were exposed to exposed to hyperbaric oxygenhyperbaric oxygenation (HBO)ation (HBO)

Research basisResearch basis: : Department of Biochemistry Department of Biochemistry and Microbiology, Southern Federal and Microbiology, Southern Federal University, Rostov-on-DonUniversity, Rostov-on-Don

In the research the experimental group of rats In the research the experimental group of rats

after the HBO-exposure (1 hour at a pressure after the HBO-exposure (1 hour at a pressure of of 00..5 5 MPa MPa in the special pressure chamber in the special pressure chamber with the alkaline absorber) underwent 5 daily with the alkaline absorber) underwent 5 daily SCENAR-sessions, 5 minute each. SCENAR-sessions, 5 minute each.

Blood plasmaBlood plasma,, erythrocyteerythrocytes, brain and liver of s, brain and liver of the animals were analyzed. the animals were analyzed.

Clinical Clinical Scientific ResearchScientific Research

Expirimental groups Expirimental groups – – patients patients underwent multiple therapy, underwent multiple therapy, including SCENAR-therapyincluding SCENAR-therapy

Control groups Control groups – – patients patients underwent only various common underwent only various common therapiestherapies

SCENAR-therapySCENAR-therapy: : General Treatment TechniquesGeneral Treatment Techniques

3 pathways and 6 points3 pathways and 6 points

andand

collar zonecollar zone, , foreheadforehead, adrenal glands, adrenal glands

Treatment techniques were alteredTreatment techniques were altered Stimulation energy – comfortableStimulation energy – comfortable

3 pathways, 6 points3 pathways, 6 points

1 2

43

5 6

Subjectively dosed mode with the Subjectively dosed mode with the labile-stabile stimulation labile-stabile stimulation techniquetechnique

3 pathways, 6 points3 pathways, 6 points

Individual dosed mode with the stabile stimulation technique based on automatic measurement of skin reactions change and evaluation of these changes

1 2

43

5 6

Collar zone, forehead, adrenal glands skin projection

123 4 5 6

Stimulation scheme and method procedure for ‘collar zone, forehead, adrenal glands’ in the SDM

with the labile-stabile stimulation

Collar zone, forehead, adrenal glands skin projection

1

2

36

5

4

1210

8

119

7

1

2

3

4

5

6

7

8

9

10

11

12

12 43 5 6

1 2

Individual dosed mode with the stabile stimulation based on automatic measurement of skin reactions change and evaluation of these changes

Research methods and testsResearch methods and testsPlasmaPlasma:: chemiluminescence analysis lipid peroxidation products content total peroxidase activity catalase and ceruloplasmin oxidase activity amount of ectoglobular hemoglobin nitrozilhemoglobin content

ErythrocyteErythrocytess:: content of lipid peroxidation products superoxide dismutase and catalase activity structural indices of erythrocyte membranes

Early postinfarction anginaEarly postinfarction angina (2003)(2003)

Experimental group (SCENAR-therapy)Experimental group (SCENAR-therapy) – 20 – 20 patientspatients

Control groupControl group – 20 – 20 patientspatientsMedical InstitutionMedical Institution : : Rostov Regional Rostov Regional

Cardiological Cardiological SanitariumSanitariumComplementary part of SCENAR-therapyComplementary part of SCENAR-therapy : :

stimulation of the neck and chest stimulation of the neck and chest anterior anterior surfacesurface, inner side of the arm and heart area. , inner side of the arm and heart area. The stimulation of the The stimulation of the abdominal wall abdominal wall was was complemented or altered in the treatment complemented or altered in the treatment (solar plexus area, lower abdomen).(solar plexus area, lower abdomen).

Treatment courseTreatment course: : 10 10 sessions with sessions with 2-3 2-3 day day breakbreak

Relapse of Relapse of duodenal ulcer duodenal ulcer (2004-2005)(2004-2005)

Experimental group (SCENAR-therapy)Experimental group (SCENAR-therapy) – – 3636 patients (monotherapy), 50 patients (multiple patients (monotherapy), 50 patients (multiple therapy)therapy)

Control groupControl group – 2– 255 patientspatientsMedical InstitutionMedical Institution : : Perm State Medical Academy Perm State Medical Academy

Complementary part of SCENAR-therapyComplementary part of SCENAR-therapy : : stimulation of the epigastrium area following stimulation of the epigastrium area following the reflexosegmental technique. the reflexosegmental technique.

Treatment courseTreatment course: : 10 10 daily sessions, 30 minutes daily sessions, 30 minutes eacheach

Acute renal failure caused by Acute renal failure caused by compression injury compression injury (2005)(2005)

Experimental group (SCENAR-therapy)Experimental group (SCENAR-therapy) – – 2525 patientspatients

Control groupControl group – – 4141 patientspatientsMedical InstitutionMedical Institution : : Department of Department of Acute Acute

HemodialysisHemodialysis, City Emergency Hospital , City Emergency Hospital №2№2, , Rostov-on-DonRostov-on-Don..

The treatment course started on patient’s The treatment course started on patient’s admissionadmission and in average included 8-10 daily and in average included 8-10 daily sessions, was finished as the diuresis sessions, was finished as the diuresis recovered.recovered.

Q-wave AMIQ-wave AMI(2007)(2007)

Experimental group (Experimental group (SCENAR-therapySCENAR-therapy)) – 30 – 30 patientspatients

Control groupControl group – – 4747 patientspatientsMedical InstitutionMedical Institution : : Cardiological Department, Cardiological Department,

City Hospital City Hospital №№2, Rostov-on-Don2, Rostov-on-Don

Complementary part of SCENAR-therapyComplementary part of SCENAR-therapy: : stimulation of the pericardium meridian (stimulation of the pericardium meridian (МС4, МС4, МС6 МС6 andand МС7 МС7) on the left and right arm for 2-) on the left and right arm for 2-3 minutes, as well as stimulation of the 3 minutes, as well as stimulation of the complaint Here and Now – treatment of the complaint Here and Now – treatment of the pain projections, if any. pain projections, if any.

Treatment course: Treatment course: 10-14 sessions, every day10-14 sessions, every day

Chronic insomniaChronic insomnia (2007) (2007) Experimental group (Experimental group (SCENAR-therapySCENAR-therapy)) – 20 – 20

patientspatientsControl groupControl group – 53 – 53 patientspatientsPatientsPatients: : emergency doctors from different emergency doctors from different

hospitals of Rostov-on-Don and Rostov regionhospitals of Rostov-on-Don and Rostov region..

Complementary part of SCENAR-therapyComplementary part of SCENAR-therapy: : stimulation of the hypersensitive zones, areas stimulation of the hypersensitive zones, areas of muscle hyper- and hypertension in the of muscle hyper- and hypertension in the zones connected with the pathological focus zones connected with the pathological focus and out of them.and out of them.

Treatment course: Treatment course: 10 sessions, every other day10 sessions, every other day

Ischemic heart diseaseIschemic heart disease (2009)(2009)Experimental group (SCENAR-therapy)Experimental group (SCENAR-therapy) – 50 – 50

patientspatients

Control groupControl group – 73 – 73 patientspatients

Medical InstitutionMedical Institution : : Cardiological Department, City Hospital №7, Rostov-on-Don

SCENAR-therapy featuresSCENAR-therapy features: : one general technique one general technique ‘3 pathways, 6 points’ in the IDM, 60Hz ‘3 pathways, 6 points’ in the IDM, 60Hz frequency. frequency.

Treatment courseTreatment course:: 10-day course, morning 10-day course, morning session every day after breakfastsession every day after breakfast

SCENAR-therapy:SCENAR-therapy:local techniqueslocal techniques

stimulation with the add-on probes stimulation with the add-on probes (12 с(12 сmm22) ) in the Fin the F--SwSw mode of skin zones in the hand area mode of skin zones in the hand area ((thenarthenar andand hypo thenarhypo thenar) ) and feetand feet ( (space under toes ofspace under toes of regio regio plantaris pedisplantaris pedis) ) withwith finalfinal stimulation of the liver stimulation of the liver projection projection ((including Fincluding F13 13 andand FF14 14 points and liver points and liver meridianmeridian)), 10 min for each zone, 10 min for each zone. .

Stimulation energy was selected individually in each Stimulation energy was selected individually in each particular case. particular case.

AppendicularAppendicular purulent peritonitispurulent peritonitis (2008)(2008)

Experimental group (Experimental group (SCENAR-therapySCENAR-therapy)) – 38 – 38 patientspatients

Control groupControl group – 42 – 42 patientspatients

Medical InstitutionMedical Institution: : Department of Department of Purulent Purulent SurgerySurgery, City Emergency Hospital , City Emergency Hospital №2№2, Rostov-, Rostov-on-Donon-Don

Treatment courseTreatment course: : 5-day course, daily morning 5-day course, daily morning sessionssessions

Including SCENAR-therapy into the multiple Including SCENAR-therapy into the multiple therapy of acute and chronic diseases therapy of acute and chronic diseases

contributes to management of oxidative stress in contributes to management of oxidative stress in the blood plasma and erythrocytes by inhibiting the blood plasma and erythrocytes by inhibiting prooxidant production and high activity of lipid prooxidant production and high activity of lipid perioxidation and regulation of the activity of perioxidation and regulation of the activity of

body antioxidant system statebody antioxidant system state

FindingFinding 1 1

Antioxidative effect is achieved by treating Antioxidative effect is achieved by treating following both general and local SCENAR-following both general and local SCENAR-

techniquestechniques

Finding 2Finding 2

Indirect antioxidative effect of Indirect antioxidative effect of SCENAR-SCENAR-THERAPY THERAPY prevails over prevails over antioxidants of direct antioxidants of direct

effect as it normalizes the balance between the effect as it normalizes the balance between the oxidative and antioxidative processes by oxidative and antioxidative processes by activating natural universal processes of activating natural universal processes of

sanogenesissanogenesis

FindingFinding 3 3

Inhibition the oxidative stress is one of the Inhibition the oxidative stress is one of the signs of evident positive clinical effect of signs of evident positive clinical effect of

SCENAR-therapySCENAR-therapy

FindingFinding 4 4

Thank YouThank You