sara a. lombardi & kennedy t. paynter marine estuarine environmental science university of...
DESCRIPTION
Differences in the gaping response and hemolymph pH of the eastern oyster Crassostrea virginica and the Asian oyster Crassostrea ariakensis when exposed to hypoxic environments. Sara A. Lombardi & Kennedy T. Paynter Marine Estuarine Environmental Science University of Maryland - PowerPoint PPT PresentationTRANSCRIPT
![Page 1: Sara A. Lombardi & Kennedy T. Paynter Marine Estuarine Environmental Science University of Maryland College Park, MD USA](https://reader035.vdocuments.site/reader035/viewer/2022081520/56816942550346895de0c96b/html5/thumbnails/1.jpg)
Differences in the gaping response and hemolymph pH of the eastern oyster
Crassostrea virginica and the Asian oyster Crassostrea ariakensis when exposed to hypoxic
environments
Sara A. Lombardi & Kennedy T. Paynter
Marine Estuarine Environmental ScienceUniversity of Maryland College Park, MD USA
![Page 2: Sara A. Lombardi & Kennedy T. Paynter Marine Estuarine Environmental Science University of Maryland College Park, MD USA](https://reader035.vdocuments.site/reader035/viewer/2022081520/56816942550346895de0c96b/html5/thumbnails/2.jpg)
Oysters and the Chesapeake Bay
Population decline of Crassostrea virginica 1% of historic population (Newell 1988)
Ecosystem service decline Before 1870: 6 day Bay filtration Now: approximately 325 days (Newell 1988)
Introduction of C. ariakensis as a solution? Increased comparisons
between the species Growth Perkinsis marinus infection (Dermo)
![Page 3: Sara A. Lombardi & Kennedy T. Paynter Marine Estuarine Environmental Science University of Maryland College Park, MD USA](https://reader035.vdocuments.site/reader035/viewer/2022081520/56816942550346895de0c96b/html5/thumbnails/3.jpg)
C. virginica and C. ariakensis during low oxygen
Harlan 2007
“C. ariakensis began gaping during the sparging process ….. The native oyster remained closed until 1-2 days before its death”
![Page 4: Sara A. Lombardi & Kennedy T. Paynter Marine Estuarine Environmental Science University of Maryland College Park, MD USA](https://reader035.vdocuments.site/reader035/viewer/2022081520/56816942550346895de0c96b/html5/thumbnails/4.jpg)
Why gape? Risk: Greater exposure to predators
Probably a physiological underpinning
Is gaping a response to release CO2? Acidic metabolic byproducts accumulating in the
hemolymph CO2 + H2O H2CO3 HCO3
- + H+
![Page 5: Sara A. Lombardi & Kennedy T. Paynter Marine Estuarine Environmental Science University of Maryland College Park, MD USA](https://reader035.vdocuments.site/reader035/viewer/2022081520/56816942550346895de0c96b/html5/thumbnails/5.jpg)
ObjectivesCompare the physiological responses of Crassostrea ariakensis and Crassostrea virginica to low oxygen
Assess gaping response after hypoxic exposure Frequency of gape Extent of gape Analyze correlations between gaping and external
water pH Inhibit gaping and assess hemolymph pH
Effect of time Effect of species
![Page 6: Sara A. Lombardi & Kennedy T. Paynter Marine Estuarine Environmental Science University of Maryland College Park, MD USA](https://reader035.vdocuments.site/reader035/viewer/2022081520/56816942550346895de0c96b/html5/thumbnails/6.jpg)
Gaping Study Methods Oysters from Horn Point Lab,
Cambridge, MD Starved for 1 week in 15ppt 25° C
water Respiration chambers filled and
sparged until oxygen concentration below 0.5mgL-1
Individual oysters were placed into a chamber which was then sealed
At periodic intervals, oyster gaping response was assessed 8-72 hours after hypoxic immersion the pH of
the water surround each oyster was analyzed.
Repeated Measure Analysis of Variance
![Page 7: Sara A. Lombardi & Kennedy T. Paynter Marine Estuarine Environmental Science University of Maryland College Park, MD USA](https://reader035.vdocuments.site/reader035/viewer/2022081520/56816942550346895de0c96b/html5/thumbnails/7.jpg)
Gaping Response
C. ariakensis gapes more often than C. virginica (p<0.0006)
12 hrs
10 days
![Page 8: Sara A. Lombardi & Kennedy T. Paynter Marine Estuarine Environmental Science University of Maryland College Park, MD USA](https://reader035.vdocuments.site/reader035/viewer/2022081520/56816942550346895de0c96b/html5/thumbnails/8.jpg)
Gape Distance
C. ariakensis gapes to a greater degree than C. virginica during early (0-72 hours) hypoxic exposure (p=0.0057)
![Page 9: Sara A. Lombardi & Kennedy T. Paynter Marine Estuarine Environmental Science University of Maryland College Park, MD USA](https://reader035.vdocuments.site/reader035/viewer/2022081520/56816942550346895de0c96b/html5/thumbnails/9.jpg)
Gaping and Water pH
Gaping is correlated with acidic external environments (p=0.034)
![Page 10: Sara A. Lombardi & Kennedy T. Paynter Marine Estuarine Environmental Science University of Maryland College Park, MD USA](https://reader035.vdocuments.site/reader035/viewer/2022081520/56816942550346895de0c96b/html5/thumbnails/10.jpg)
Hemolymph pH Methods Clamped using 2” binder clips and
placed on lab bench at ~25° C air temperature
Sacrificed at hr 0 (control), 2, 4, 6, 8, 10, 12, & 24
Dorsal and ventral side drilled Hemolymph sampled with 5ml glass
syringe pH analyzed with bench top meter
and micro pH probe Performed Perkinsis marinus analysis
Excluded those with P. marinus score greater than 1 from analysis
Two-way ANOVA
![Page 11: Sara A. Lombardi & Kennedy T. Paynter Marine Estuarine Environmental Science University of Maryland College Park, MD USA](https://reader035.vdocuments.site/reader035/viewer/2022081520/56816942550346895de0c96b/html5/thumbnails/11.jpg)
Hemolymph pH after clamping
Species: p<0.0001Time: p<0.0001Interaction: p=0.2934
![Page 12: Sara A. Lombardi & Kennedy T. Paynter Marine Estuarine Environmental Science University of Maryland College Park, MD USA](https://reader035.vdocuments.site/reader035/viewer/2022081520/56816942550346895de0c96b/html5/thumbnails/12.jpg)
Change in hemolymph pH after clamping
Species: p= 0.0214Time: p<0.0001Interaction: p=0.2934
**
![Page 13: Sara A. Lombardi & Kennedy T. Paynter Marine Estuarine Environmental Science University of Maryland College Park, MD USA](https://reader035.vdocuments.site/reader035/viewer/2022081520/56816942550346895de0c96b/html5/thumbnails/13.jpg)
Conclusions C. ariakensis gapes more often than C. virginica
(p<0.0006)
C. ariakensis gapes to a greater extent than C. virginica (p=0.0057)
Gaping is correlated with acidic external environments (p=0.034)
When gaping is prevented…
C. ariakensis hemolymph pH is more acidic than C. virginica (p<0.0001)
![Page 14: Sara A. Lombardi & Kennedy T. Paynter Marine Estuarine Environmental Science University of Maryland College Park, MD USA](https://reader035.vdocuments.site/reader035/viewer/2022081520/56816942550346895de0c96b/html5/thumbnails/14.jpg)
Conclusions and Implications
C. ariakensis may respond differently to low oxygen and different acid-base homeostatic abilities
Gaping may be a mechanism to prevent or limit metabolic acidosis in C. ariakensis
![Page 15: Sara A. Lombardi & Kennedy T. Paynter Marine Estuarine Environmental Science University of Maryland College Park, MD USA](https://reader035.vdocuments.site/reader035/viewer/2022081520/56816942550346895de0c96b/html5/thumbnails/15.jpg)
Next steps Assess pH over time simultaneously with
calcium and carbon dioxide concentration Assess hemolymph pH of gaping and
ungaped oysters when exposed to low oxygen Effect of gaping on hemolymph pH
Assess the effect of acidosis on adductor muscle function
![Page 16: Sara A. Lombardi & Kennedy T. Paynter Marine Estuarine Environmental Science University of Maryland College Park, MD USA](https://reader035.vdocuments.site/reader035/viewer/2022081520/56816942550346895de0c96b/html5/thumbnails/16.jpg)
References Acknowledgements
Funding Oyster Recovery Partnership National Oceanographic and
Atmospheric Administration – Chesapeake Bay Office
Army Corp of Engineers – Baltimore district
University of Maryland’s Horn Point Lab Oyster Hatchery Dr. Donald Merrit Stephanie Tobash Alexander
University of Maryland Paynter lab staff and
students
Dwyer J. J & Burnett L.E 1996. Acid-base status of the oyster Crassostrea virginica in response to air exposure and to infections by Perkinsus marinus. Biol. Bull. 190 :13-137
Newell R. I. E 1998. Ecological changes in Chesapeake Bay: Are they the result of overharvesting the American oyster, Crassostrea virginica? Understanding the estuary: Advances in Chesapeake bay research. Proceedings of a conference 29-31. Chesapeake Research consortium publication 129.
Harlan N.P. 2007 A comparison of the physiology and biochemistry of the eastern oyster, Crassostrea virginica, C. ariakensis. Masters of Science.
Stickle W.B., Kapper M.A, Liu L., Gnaiger E., & Wang S.Y. 1989. Metabolic adaptations of several species of crustaceans and Molluscs to Hypoxia: Tolerance and Microcalorimetric studies. Biological Bulletin 177:303-312