7/21/2019 S2-2014-337575-abstract

http://slidepdf.com/reader/full/s2-2014-337575-abstract 1/1

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ABSTRACT

The method of choice for analysis of drugs in multi-component preparations is chromatographic based technique such as high performance liquid

chromatography (HPLC). However, chromatographic method is time consuming

and requiring much effort. As a consequence, some simple methods such as Uv

spectrophotometry are continuously developed, especially in combination with the

chemometrics software. UV-vis spectrophotometry coupled with multivariate

calibration of partial least square (PLS) has been developed for quantitative

analysis of paracetamol, guaiphenesin and chlopheniramine maleate in the

 presence of phenylpropanolamine without separation step.

The calibration model is prepared by developing a series of sample

mixture comprising these drugs in certain proportion within the range 0  –   20

µg/mL. The evaluation of calibration model was based on coefficient ofdetermination (R 2), root mean square error of calibration (RMSEC), root mean

square error of cross validation (RMSECV), root mean square error of prediction

(RMSEP) and predicted residual sum of square (PRESS). The result showed that

Uv spectrophotometry combined with PLS can be used for quantitatve analysis of

drugs. The coefficient of determination (R 2) for the relationship between actual

values and predicted values was higher than 0.99 indicating good accuracy. The

RMSEC, RMSECV, RMSEP and PRESS values obtained were relatively low

indicating good precision.

The levels of PCT, GG and CTM obtained by uv spectrophotometry in

combination with PLS are 639.38 ± 5.23 mg/tab; 102.61 ± 1.48 mg/tab and 1.98 ±

0.0052 mg/tab respectively, and those by HPLC are 648.61 ± 7.58 mg/tab; 101.07± 0.80 mg/tab and 2.12 ± 0.03 mg/tab recpectively. The precision and accuracy of

developed method was compared to that of HPLC statistically resulting not

significantly different on precision but significantly different on accuracy. The

developed method was valid and succesfully used for analysis of paracetamol,

guaiphenesin and chlorpheniramine maleate in synthetic mixture and tablet

dosage form.

Keywords: Uv-vis spectrophotometry, multivariate calibrations, multi-component

drugs.