running gc. 1.launch the “totalchrom” software 2.click on “run” 3.select “take...
TRANSCRIPT
Running GC
Running GC1. Launch the
“TotalChrom” Software
2. Click on “Run”3. Select “Take Control…”4. Select “Clarus 580”5. Click “OK”
Running GC
• The GC touch screen panel should show that the GC is in external control.
• Once in external control:• Build Method• Build Sequence
Build Method
Build Method
1. Click on “Method” button
Build Method
2. Select “Create new method”
3. Click on “OK”
Build Method4. Type in a description5. Click “Next”
6. Type in a header text7. Click “Next”
• The Data Acquisition window should look like the following
8. Click “Next”
Build Method
• Autosampler tab should look like the following
• Adjust as you see fit• What is shown is a good
standard 9. Click “Oven/Inlets” Tab
Build Method
10. Adjust the Oven Ramp program as directed.• Make sure to hit enter
on the keyboard after every entry
11. Adjust the “Injector A” temperature. Typically this is set around 225 C.
12. Click on “Carrier” Tab
Build Method
13. Adjust the Carrier tab as directed.• Typical settings are
shown.14. Click on “Detectors” Tab
Build Method
15. Adjust the Detectors tab as directed.• Typical settings are
shown.16. Click on “Next”
Build Method
Click “Next”
Click “Next”
Click “Next”
(No Need to Mess with)
Click “Finish”
Build Method
17. File Save18. Create a unique name19. Click on “Save”20. Close Method window
Build Sequence
Build Sequence
1. Click on “Sequence” button
Build Sequence
2. Select “Create new sequence”3. Click on “OK”
Build Sequence
4. Select the listed options5. Click on “OK”
Build Sequence6. Type in a unique name7. Type in which vial to be used8. Select a method (brings up a
new window)9. Click on “Add+Close” if all vials
are added or click on “Add” to add more vials
Build Sequence
10. File Save11. Create a unique name12. Click on “Save”13. Close Sequence window
Running Sequence
Running Sequence
1. Click on “Setup” button
Running Sequence
1. Select a sequence (new window will open / not shown)
2. Click OK
Running Sequence• The GC will take some
time to upload the sequence information, equilibrate, and do pre-run operation
• You will know that the sequence upload properly because the Instrument-Hands On and Instrument-Modify will no longer be grayed out
• Once the everything is ready the lettering here should be in green.
• I/F will read Ready3. Click on Run4. Start Run