Gov. science

college

Biotech

sem3

Khushal

khambhati

Roll no. 18

• the removal of introns from a primary transcript and the subsequent joining of exons in the production of a mature RNA molecule.

RNA splicing

transcriptio

n

exons

intron

s

Rna

splicingtranslation

DNA PRE-

MRNA

MATURED

RNA Polypeptid

e

historyphillip Allen Sharp is an American geneticist and molecular biologist who co-discovered RNA splicing. He shared the 1993 Nobel Prize with Richard J. Roberts for "the discovery that genes in eukaryotes are not contiguous strings but contain introns, and that the splicing of messenger RNA to delete those introns can occur in different ways, yielding different proteins from the same DNA sequence".

phillip Allen Sharp Richard J. Roberts

1 2 4 5 6 8 9 103 7

1 2 5 7 6 8 9 104 5 1 4 6 4

1 2 4 5 6 8 9 103 7

1 4 5 2 1 4 6 7 6 4 8 9 105

1 2 5 7 6 8 9 104 5 1 4 6 4

1 4 5 2 1 4 6 7 6 4 8 9 105

Splicing

site

GU site

Branch sitePyridines

track

Splicing

site

AG site

BREAKING

PHOSPHODIESTE

R BOND DUE TO

ADDITION OF –OH

GROUP

FORMATION OF

PHOSPHODIESTE

R BOND –

formation of loop

but what

would

help to do

these

Spliceoso

me

• It is a large and complex molecular machine which helps to remove the introns from pre-Mrna molecule

Spliceosome

●The complexes of snRNA and

proteins are called small nuclear

ribonuclear proteins (snRNP,

pronounces “snurps”).

There are 5 snrnp’s

U1, U2, U4, U5, U6

● Spliceosome act by formation

of-

COMPLEX E

COMPLEX A

COMPLEX B

COMPLEX C

u1

u2

u4

u5

u6

E complex

a complex

a complex

b complex

b complex

c complex

u1 bbpu2af65

u2af36u1

u1

u1

u1bbp

u1

u2

u2

u2af65u2af36

u2

U6, u4, u5

u4

U6, u4, u5

u2

Rna splicing

IntroductionWhy intron

are important

process Spliceosome Complex

Reference

http://genetics.thetech.org/ask/ask326

http://www2.oakland.edu/biology/chaudhry/pics/chapter24f.pdf

http://www.dnalc.org/resources/animations/rna-splicing.html

http://highered.mheducation.com/olcweb/cgi/pluginpop.cgi?it=swf::535::535::/site

s/dl/free/0072437316/120077/bio30.swf::How+Spliceosomes+Process+RNA

http://www.evolutionnews.org/2013/09/the_spliceosome_1076371.html

http://www.evolutionnews.org/2011/12/finding_treasur054241.html