reversed-phase c18 columns (ods)

YMC GENERAL CATALOG

1Reversed-PhaseC18 Columns (ODS)

Types and characteristics of C18packing materials

Guides for selecting C18 packing materials

YMC-Triart C18

YMC-UltraHT series 　YMC-UltraHT series 　YMC-UltraHT Pro C18 　YMC-UltraHT Hydrosphere C18

Pro series 　Features of Pro series ODS columns 　YMC-Pack Pro C18 　Hydrosphere C18 　YMC-Pack Pro C18 RS

YMC-Pack ODS series 　YMC-Pack ODS-A 　YMC-Pack ODS-AM 　YMC-Pack ODS-AQ 　YMC-Pack ODS-AL

J,sphere ODS series 　ODS-H80、M80、L80

YMC-Pack PolymerC18

14

15

16～23

24,252627

28～3132～3536～3940～43

44,4546,4748,4950,51

52,53

54,55

13

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Types of C18 packing materialsBase Products

Hybrid silica YMC-Triart C18

Silica

YMC-UltraHT seriesYMC-Pack Pro C18Hydrosphere C18YMC-Pack Pro C18 RSYMC-Pack ODS seriesJ’sphere ODS series

Polymer YMC-Pack PolymerC18

Types and characteristics of C18 (ODS) packing materials

Product namePore size（Å）

Particle size

（µm）

ODSUsable

pH range Characteristics PagesC% Silanol

treatment

YMC-Triart C18120

2

20 Yes 1.0 〜12.0

・ Versatile hybrid silica based ODS column

・Great chemical durability・ Excellent peak shape in every conditions

16 〜 233

5

UltraHT seriesPro C18 120 2 16

Yes 2.0 〜 8.0

・ 2 µm-ODS column for Ultra-fast LC method

・ Superior separation of basic compounds

26

Hydrosphere C18 120 2 12

・ Superior separation of hydrophilic compounds

・ Reduced analysis time while maintaining resolution

27

Pro seriesPro C18 120

316 Yes

2.0 〜 8.0

・ Processed with YMC Co., Ltd.’s advanced endcapping technology

・ Superior separation of basic compounds32 〜 35

5

Hydrosphere C18 1203

12 Yes

・ Superior separation of hydrophilic compounds

・ Can be used with 100% water mobile phase

36 〜 395

Pro C18 RS 803

22 Yes 1.0 〜10.0・Highly durable ODS・ Superior separation of basic compounds

and hydrophobic compounds40 〜 43

5

YMC-Pack ODS series

ODS-A

1203

17

Yes

2.0 〜 7.5

・ Currently in use worldwide 44,45510

200 5 12 ・ODS with wide pore size・ For separation of peptides

and proteins44,4510

300 5 710

ODS-AM 120 3 17 Yes ・ Outstanding lot-to-lot reproducibility 46,475

ODS-AQ120

314

Yes ・ Superior separation of hydrophilic compounds 48,49

510

200 5 1010ODS-AL 120 5 17 No ・ For separation utilizing residual silanol 50,51

J'sphere ODS series ODS-H80 80 4 22 Yes 1.0 〜 9.0 ・High carbon ODS52,53ODS-M80 80 4 14 Yes 2.0 〜 7.5 ・Medium carbon ODS

ODS-L80 80 4 9 Yes ・Low carbon ODSYMC-Pack PolymerC18 — 6 — — 2.0 〜13.0 ・Polymer-based ODS 54,5510 —

Types and characteristics of C18 packing materialsOctadecyl-silica (ODS) is the industry standard packing material for HPLC applications. YMC Co., Ltd. offers packing materials with an impressive assortment of functional groups for liquid chromatography and a selection of ODS columns that is far ahead of all others in variety. We also offer hybrid-silica based C18 packing materials that are widely applicable to a variety of fields, from microanalysis to large-scale isolation.

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Low molecular weight compounds

High molecular weight compounds

Alkaline mobile phase

General organic compounds

Basic compounds

Coordination compounds

Sugars and glycosides

YMC-Triart C18YMC-Pack Pro C18YMC-Pack ODS-AJ’sphere ODS-M80

YMC-Pack Pro C18 RSJ’sphere ODS-H80

YMC-Pack ODS-A (Wide-Pore)J’sphere ODS-L80

Hydrosphere C18YMC-Pack Pro C18 RSYMC-Pack ODS-ALJ’sphere ODS-H80J’sphere ODS-L80YMC-Pack PolymerC18

YMC-Triart C18YMC-Pack Pro C18Hydrosphere C18YMC-Pack Pro C18 RSYMC-Pack ODS-AMYMC-Pack PolymerC18

YMC-Triart C18YMC-Pack Pro C18Hydrosphere C18YMC-Pack Pro C18 RSYMC-Pack ODS-AMYMC-Pack PolymerC18

Hydrosphere C18YMC-Pack ODS-AQJ’sphere ODS-L80

YMC-Pack ODS-A (Wide-Pore)

YMC-Triart C18YMC-Pack Pro C18 RSJ’sphere ODS-H80YMC-Pack PolymerC18

Chemical structures differ slightly among solutes

Hydrophobicity differs greatly among solutes

Difficult to separate using standard ODS

High molecular weight peptides and oligonucleic acids

Guides for selecting C18 packing materialsYMC Co., Ltd. offers twelve types of ODS columns, each with unique separation characteristics. The proper selection of packing material is therefore a key factor in the establishment of efficient separation conditions. Selection of packing materials without sufficient consideration of the separation characteristics may result in an expense of much time and effort in separating a target material.

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Versatile Hybrid Silica Based HPLC Column

YMC-Triart C18YMC-Triart C18 is a hybrid silica based ODS column emphasizing durability, low operating pressure, and excellent performance.YMC-Triart C18 has been developed and is manufactured using state-of-the-art technologies that define materials, granulation and surface modification.

YMC-Triart C18　－Integration of triple artistic technologies－

＜1＞Versatile hybrid material

YMC-Triart C18 is a multi-layer hybrid particle consisting of two types of layers: a silica based layer (inorganic) and a hybrid polymer based layer (organic). This layered structure contributes to Triart's combined physical durability and chromatographic performance.

Image structurefor hybrid-silica gel

inorganic-silane layer

organic-silane layer

Particlesynthesis byMicroreaction

Particlesynthesis byMicroreaction

Versatilehybrid silica

material

Versatilehybrid silica

material

Multi-stageendcappingMulti-stageendcapping

Rugged, long lasting, hybrid particle technologyIntegration of triple artistic technologies

SpecificationsBase ：organic/inorganic hybrid silicaStationary ：C18 (as USP-L1)Particle ：2 µm, 3 µm, 5 µmPore ：120 Å

Carbon % ：20%Bonding ：polymeric typeEndcapping ：multi-stage endcappingpH range ：pH 1〜 12

Features

● Hybrid silica base ▼ Usable over a wide pH range makes the column excellent for method development.

● Great chemical durability ▼ Long column lifetime over wide pH range. 　Low cost per analysis.

● Lower back pressure ▼ Ability to use on both older and newer instrumentation.

● Excellent resolution ▼ Rugged, first choice option for analysis of acids, bases and neutral compounds.

Benefit

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YMC-Triart C18 has a very narrow pore size distribution that enables narrow and reproducible peak shapes for analytes.

Narrow pore distribution

Silica particle for conventional product

350300250200Pore size (Å)

150100500

4500

4000

3500

3000

2500

2000

1500

1000

500

0

d V

p/d

logD

Hybrid particle for competitor's product

Hybrid particle for Triart

＜2＞New granulation process by microreactor technology

Homogeneous and uniform particle

Use of microreactor technology produces homogeneous and uniform particles that results in low operating pressure and very reproducible surface modification making for the ultimate in column-to-column and lot-to-lot reproducibility.

Triart C18

YMC-Triart C18　－Integration of triple artistic technologies－

Column ：5 µm, 150×3.0 mmI.D.Eluent ：20 mM KH2PO4-K2HPO4 (pH 6.9)/acetonitrile (65/35)Flow rate ：0.425 mL/minTemperature ：40 ℃Detection ：UV at 235 nmSample ： 1. Chlorpheniramine　　2. Dextromethorphan

3. Propyl p-hydroxybenzoate

Endcapping methods employed for production of conventional products typically employ a single endcapping compound in one chemical step to "neutralize" highly reactive silanols. Unfortunately, highly reactive silanols are easily hydrolyzed and contribute to poor durability. Additionally, low reactive silanols endcap stubbornly, producing a surface modification that is often non-reproducible, and leading to peak tailing. YMC-Triart C18 solves this problem by using an innovative, newly developed "measured multi-stage/multi-compound surface modification" technology which results in the thorough endcapping of all silanols that are accessible to analytes.

＜3＞Surface modification technology

Multi-stage endcapping

Reaction image for "multi-stage endcapping"

3STEP

Silanol group

Endcapping (EC) group

1STEP

2STEP

BeforeEC

Evaluation of silanol activity

12

3

Triart C18

Endcappingby traditional method

Peak tailing for basic compounds

min15.010.05.00.0

mAU

60

1

2

3

Q090928E

Q090928C

40

20

0

min15.010.05.00.0

mAU

60

40

20

0

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YMC-Triart C18

Quality Control　－Excellent reproducibility－

1. Chlorpheniramine3. Propyl p-hydroxybenzoate

2. Dextromethorphan

Basic compounds

Lot. E

Lot. D

Lot. C

Lot. B

Lot. Amin17.515.012.510.07.55.02.50.0

13

2

J091201Y

YMC-Triart C18 exhibits excellent lot-to-lot reproducibility for all types of compounds including difficult to chromatograph molecules that exhibit basic and coordination compound behavior.

Packing Material

Rigid control of theoretical plate number (N) and tailing factor (Tf) are exhibited for each YMC-Triart C18 packed column.

Packed Column

Lot. E

Lot. D

Lot. C

Lot. B

Lot. Amin14.012.010.08.06.04.02.00.0

1 2

J091201X

1. Hinokitiol2. Methyl benzoate

20000

15000

10000

5000

0

2.5

2.0

1.5

1.0

0.510 20 30 40 50

Theoretical plate number

Tailing factor

Column number

Theo

retic

al p

late

num

ber

Taili

ng fa

ctor

Column ：5 µm, 150×3.0 mmI.D.Eluent ：20 mM KH2PO4-K2HPO4 (pH 6.9)/acetonitrile (65/35)Flow rate ：0.425 mL/minTemperature ：40 ℃Detection ：UV at 235 nm

Column ：5 µm, 150×3.0 mmI.D.Eluent ：acetonitrile/0.1% H3PO4 (40/60)Flow rate ：0.425 mL/minTemperature ：40 ℃Detection ：UV at 254 nm

Column ：5 µm, 150×4.6 mmI.D.Eluent ：acetonitrile/water (60/40)Flow rate ：1.0 mL/minTemperature ：ambientSample ：butyl benzoate

Low back-pressure　－Free from pressure problem－Column pressure and solvents

YMC-Triart C18 is designed for use in all conditions. Even with higher viscosity methanol, YMC-Triart C18 can operate with lower pressure (typically 30% less than conventional columns).

Column ：5 µm, 150×4.6 mmI.D.Eluent ：acetonitrile/water or methanol/waterFlow rate ：1.0 mL/minTemperature ：25 ℃

0 10 20 30 40 50 60 70 80 90 100

Organic solvent rate (%)

Col

umn

pre

ssur

e (M

Pa)

0

2

4

6

8

10

12

14

16

18

20

Triart C18, ACN/W

Pro C18, ACN/W

Triart C18, MeOH/W

Pro C18, MeOH/W

Standard ODS　－Best balance hydrophobicity－Comparison of separation selectivity

Conventional hybrid silica based ODS columns tend to be less hydrophobic than silica based columns. YMC-Triart C18 has a favorable carbon balance and may be used as a "versatile first-choice" method development column.

XTerra MS C18

(S090805A)

Standard ODS

Gemini NX-C18

2.00 4.00 6.00 8.00 10.00 12.00 14.00

Triart C18

Hyd

roge

n-b

ond

ing

cap

acity

α (c

affe

ine/

ben

zene

)

Hydrophobicity k’ (amylbenzene)

The most versatilecolumns as first-choice.

Inertsil ODS-4

Eluent : MeOH/W (80/20) [amylbenzene], MeOH/W (30/70) [caffeine, benzene]

Pro C18 RS

Develosil ODS-SR

Develosil C30-UG

Atlantis T3

Hydrosphere C18

CAPCELL PAK C18 AQ

Pro C18Gemini C18

Hypersil GOLD

XBridge C18Eternity-C18

Cadenza CD-C18L-column2 ODSTSKgel ODS-100S

L-column ODSMightysil RP-18

SunFireC18

Inertsil ODS-3

CAPCELL PAK C18 MGII

CAPCELL PAK C18 MGIII

Unison UK-C18

0.040

0.060

0.080

0.100

0.120

0.140

0.160

0.180


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Great durability　－Wide usable pH range and long lifetime－

Phosphate buffer (pH 11.5, 40 ℃)

0 10 20 30 40 50

Time (hr)

Rat

e of

the

oret

ical

pla

te n

umb

er YMC-Triart C18

Silica based C18Hybrid-silica based C18

0%

20%

40%

60%

80%

100%

120%

Durability in high pH

Durability in high temperature

0 30025020015010050

Time (hr)

Rat

e of

the

oret

ical

pla

te n

umb

er

0%

20%

40%

60%

80%

100%

120%

YMC-Triart C18

Silica based C18

Column ：5 µm, 150×4.6 mmI.D.Eluent ：50 mM K2HPO4-K3PO4 (pH 11.5)/methanol (90/10)Flow rate ：1.0 mL/min Temperature ：40 ℃Sample ：benzyl alcohol

Column ：5 µm, 150×4.6 mmI.D.Eluent ：50 mM triethylamine (pH 11.5)/methanol (90/10)Flow rate ：1.0 mL/minTemperature ：40 ℃Sample ：benzyl alcohol

YMC-Triart C18 shows great chemical stability provided by newly developed hybrid silica. Even in high pH or high temperature conditions, the lifetime of YMC-Triart C18 is more than 10 times greater than conventional ODS columns.

YMC-Triart C18

Time (hr)

Rat

e of

the

oret

ical

pla

te n

umb

er

Silica based C18

Silica based C18alkaline-resistant type

0%

20%

40%

60%

80%

100%

120%

0 100 200 300 400 500 600 700 0 12010080604020

Time (hr)

Rat

e of

ret

entio

n tim

e

0%

20%

40%

60%

80%

100%

120%

YMC-Triart C18

Silica based C18

The columns are kept in acetonitrile/water/TFA (10/90/1), 70 °C,and tested for performance every 20 hours.

Hybrid-silica based C18

Column ：5 µm, 50×2.0 mmI.D.Eluent ：20 mM KH2PO4-K2HPO4 (pH 6.9)/acetonitrile (90/10)Flow rate ：0.2 mL/minTemperature ：70 ℃Sample ：phenol

Test conditions Column ：5 µm, 50×2.0 mmI.D. Eluent ：acetonitrile/water (60/40) Flow rate ：0.2 mL/min Temperature ：37 ℃ Sample ：butyl benzoate

Reduced column bleed　－Operate at high sensitivity－Bleed test by LC/MS

No column

Triart C18Scan ES+TIC

0.0E+00

4.0E+08

8.0E+08

1.2E+09

min121086420

－

－

Bleeding of stationary phase can often contribute to high background signals when employing MS and other hyphenated detection techniques. In the example cited above, use of a 50×2.0 mmI.D. YMC-Triart C18 column shows no contribution to the signal observed by the MS positive total ion current (TIC) trace. Such performance allows for low limits of detection (high S/N ratio) for MS and other detection techniques. The inherent hybrid particle strength is also superior to conventional silica materials for detection techniques that are sensitive to particle degradation.

Column ：5 µm, 50×2.0 mmI.D.Eluent ： A) water/formic acid (100/0.1)

B) acetonitrile/formic acid (100/0.1) 5% B (0-1 min), 5-100% B (1-5 min), 100% B (5-10 min), 100-5% B (10-10.1 min), 5% B (10.1-12.5 min)

Flow rate ：0.4 mL/minTemperature ：40 ℃Detection ：ESI positive, TIC (Mass Range: 50-1000)

Triethylamine (pH 11.5, 40 ℃)

pH 6.9, 70 ℃ pH 1, 70 ℃

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YMC-Triart C18

Excellent resolution　－Without adsorption and tailing－Analysis of basic compounds

Hybrid-silica based C18Brand I8

5 µm, 150×4.6 mmI.D.

Silica based C18Brand D5

5 µm, 150×4.6 mmI.D.

Silica based C18Brand N1

3 µm, 150×3.0 mmI.D.

YMC-Triart C185 µm, 150×3.0 mmI.D.

0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min

21

3

21

3

2

1

3

2

13

Q090928C

Q090123F

Q090821C

J091111K

Column ：150×3.0 mmI.D. or 150×4.6 mmI.D.Eluent ：20 mM KH2PO4-K2HPO4 (pH 6.9)/acetonitrile (65/35)Flow rate ： 0.425 mL/min for 3.0 mmI.D.

1.0 mL/min for 4.6 mmI.D.Temperature ：40 ℃Detection ：UV at 235 nm

Ingredients in a cough/cold medication

N

N

CH3

H3C

Cl

H3C

O

O

OH

O

NH

CH3

CH3

Chlorpheniramine

Propyl p-hydroxybenzoate

Dextromethorphan

1.

3.

2.

YMC-Triart C18's innovative surface modification technology results in excellent peak shapes even for the basic compounds that often exhibit tailing shapes on conventional silica and hybrid silica based ODS columns.

Hybrid-silica based C18Brand I8

5 µm, 150×4.6 mmI.D.

Silica based C18Brand N1

3 µm, 150×3.0 mmI.D.


0.0 2.0 4.0 6.0 8.0 10.0 12.0 14.0 min

2

1

21

2

1

Q090925G

Q090120A

J091110B

Column ：150×3.0 mmI.D. or 150×4.6 mmI.D.Eluent ：acetonitrile/0.1% H3PO4 (40/60)Flow rate ： 0.425 mL/min for 3.0 mmI.D.


Hinokitiol

O OCH3

OH

CH3

CH3

O

Methyl benzoateHinokitiol

2.1.

YMC-Triart C18 has an extremely low level of metal impurities, much lower than conventional products.YMC-Triart C18 is able to provide excellent peak shape for coordination compounds.

Hybrid-silica based C18Brand G4

5 µm, 150×4.6 mmI.D.


0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 min

1

2

3

1

2

3

Q090605C

Q090717A

Column ：150×3.0 mmI.D. or 150×4.6 mmI.D.Eluent ：acetonitrile/0.1% H3PO4 (5/95)Flow rate ： 0.425 mL/min for 3.0 mmI.D.


YMC-Triart C18 is synthesized utilizing methodology borrowed from microreactor technology. This synthesis technique allows for a reduction of impurities that contribute to peak tailing during the analysis of some types of acidic compounds.

Organic acid1. Formic acid2. Acetic acid3. Propionic acid

Analysis of coordination compounds

Analysis of acidic compounds

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Excellent versatility　－Buffer independency－Comparison of clemastine analysis with conventional columns

YMC-Triart C1850×2.0 mmI.D.

methanol acetonitrile

Silica based C18Brand C150×2.1 mmI.D.

Silica based C18Brand D550×2.0 mmI.D.

Silica based C18Brand I650×2.1 mmI.D.

Hybrid-silicabased C18Brand I8 50×2.1 mmI.D.

methanol acetonitrile

Tf 0.97

Tf 1.21

Tf 0.93

Tf 1.03

Tf 1.14

Tf 1.11

Tf 1.56

Tf 2.83

Tf 3.87

Tf 1.88

Tf 1.03

Tf 1.13

Tf 1.05

Tf 1.13

Tf 1.22

Tf 1.16

Tf 1.43

Tf 2.21

Tf 1.88

Tf 1.78

10.0 11.0 12.0 6.0 7.0 8.0 8.0 8.0 min7.06.09.0 10.0 minminmin

F090731H07 F090731I07 F090731F07 F090731G07

F090722H07 F090722I07 F090722F07 F090722G07

F090714G07F090714F07F090717I07F090717H07

F090803G07F090727F07F090727I07F090727H07

F090817G07F090818F07F090818I07F090818H07

10 mM phosphate buffer (pH 6.7)/organic solvent 10 mM CH3COONH4/organic solvent

Clemastine is a well known basic compound which can easily tail on conventional ODS columns. YMC-Triart C18 can separate clemastine sharply using many buffer/solvent combinations.

Clemastine

N O

Cl

H3CCH3

Column ：5 µm, 50×2.0 mmI.D. or 50×2.1 mmI.D.Eluent ： A) 10 mM KH2PO4-K2HPO4 (pH 6.7) or 10 mM CH3COONH4 (pH 6.7)

B) methanol or acetonitrile 5-90% B (0-10 min), 90% B (10-15 min)

Flow rate ：0.2 mL/minTemperature ：25 ℃Detection ：UV at 230 nm

Improvement of loadability　－Minimizing strong solvent/sample loading effects－Influence on peak shapes by injection volume

1

2

1 2

min1.5 2.0 2.5

Hybrid-silica based C18

Silica based C18

YMC-Triart C181

2

Peak anomaly at 1.5 µL

YMC-Triart C18 can tolerate larger injection volumes of samples containing strong solvents (e.g., acetonitrile) while allowing for better peak shape than traditional columns. This can be important for difficult to solubilize samples that need higher concentrations of organic solvent for solubilization prior to analysis.

Column ：5 µm, 50×2.0 mmI.D. or 2.1 mmI.D.Eluent ： A) water/formic acid (100/0.1)

B) acetonitrile/formic acid (100/0.1) 5% B（0-0.5 min）, 5-100% B (0.5-2.5 min)


Propranolol (50 µg/mL) Verapamil (50 µg/mL)

1. 2.OCH2CHCH2NHCH(CH3)2

H3CO

H3CO

CH(CH3)2

CN CH3OCH3

OCH3C (CH2)3NCH2CH2OH

acetonitrile 1.0 µL1.5 µL2.0 µL3.0 µL

solvent for sample injection volume

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YMC-Triart C18

YMC-Triart C18 3 µm, 50×2.0 mmI.D.

80% reduction in run time

5%B, 0.4 mL/min, 12.2 MPa

1

2

R091201B

mAU

25

20

15

10

5

0

0 1 2 3 4 5 min

Baseline separation,But long analysis time

5%B, 0.2 mL/min, 5.6 MPa

12

R091127C

mAU

25

20

15

10

5

0

0 5 10 15 20 25 min


Fast analysis of paraquat and diquat

Insufficientseparation

10%B, 0.2 mL/min, 5.7 MPamAU

25

20

15

10

5

0

0 2 4 6 8 10 12 14 min

12

R091126J


Eluent ： A) water/HFBA* (100/0.1) B) acetonitrile/HFBA* (100/0.1)

Temperature ：37 ℃Detection ：UV at 290 nmInjection ：1 µL (0.1 mg/mL)*heptafluorobutyric acid

Diquat

N+N+·2Br− N+N+H3C CH3

·2Cl−

Paraquat

1. 2.

Downsizing

Eluent optimization

Column ： YMC-Triart C18 (5 µm, 120 Å) 250×4.6 mmI.D.

Eluent ： A) water/formic acid (90/10) B) acetonitrile/methanol/water/formic acid 　 (22.5/22.5/40/10) 20-28% B (0-30 min), 28-70% B (30-40 min), 100% B (40-45 min)

Flow rate ：1.0 mL/minTemperature ：25 ℃Detection ：UV/VIS at 535 nmSample ：commercial bilberry powder (1.25 mg/mL)

12

3

4

5

6

7

8

9

1011

12

13

14

min0 5 10 15 20 25 30 35 40

mAU

0

20

40

60

80

100

15

1617

18

19

20

Anthocyanin 1. Delphinidin-3-O-galactoside 2. Delphinidin-3-O-glucoside 3. Cyanidin-3-O-galactoside 4. Delphinidin-3-O-arabinoside 5. Cyanidin-3-O-glucoside 6. Petunidin-3-O-galactoside 7. Cyanidin-3-O-arabinoside 8. Petunidin-3-O-glucoside 9. Peonidin-3-O-galactoside10. Petunidin-3-O-arabinoside11. Delphinidin12. Peonidin-3-O-glucoside13. Malvidin-3-O-galactoside14. Peonidin-3-O-arabinoside 15. Malvidin-3-O-glucoside16. Malvidin-3-O-arabinoside17. Cyanidin18. Petunidin19. Peonidin20. Malvidin

A p p l i c a t i o n （Q091110E）

第01章.indd 22 11.1.28 10:39:38 AM

23

1

Reversed-P

hase C1

8 C

olumns

（OD

S

）

0 5 10 15 20 min

0

25

50

75

100

mAU

1 6

5

4

3

2

25

Peptide1. Oxytocin (MW 1,007)2. Met-Enkephalin (MW 574)3. Leu-Enkephalin (MW 556)4. Neurotensin (MW 1,673)5. γ-Endorphin (MW 1,859)6. β-Endorphin (MW 3,465)


Eluent ： A) water/TFA (100/0.1) B) acetonitrile/TFA (100/0.1) 20-45% B (0-25 min)


A p p l i c a t i o n （R091009C）

Ordering information

Analytical columnsParticle size（µm）

Pore size（Å）

Column sizeinner diameter×length（mm） Product number

S-3 120 2.0× 20 TA12S03-0202WT2.0× 30 TA12S03-0302WT2.0× 50 TA12S03-0502WT2.0× 75 TA12S03-L502WT2.0×100 TA12S03-1002WT2.0×150 TA12S03-1502WT3.0× 50 TA12S03-0503WT3.0×100 TA12S03-1003WT3.0×150 TA12S03-1503WT4.6× 35 TA12S03-H546WT4.6× 50 TA12S03-0546WT4.6× 75 TA12S03-L546WT4.6×100 TA12S03-1046WT4.6×150 TA12S03-1546WT

S-5 120 2.0× 20 TA12S05-0202WT2.0× 30 TA12S05-0302WT2.0× 50 TA12S05-0502WT2.0× 75 TA12S05-L502WT2.0×100 TA12S05-1002WT2.0×150 TA12S05-1502WT3.0× 50 TA12S05-0503WT3.0× 75 TA12S05-L503WT3.0×100 TA12S05-1003WT3.0×150 TA12S05-1503WT4.6× 50 TA12S05-0546WT4.6× 75 TA12S05-L546WT4.6×100 TA12S05-1046WT4.6×150 TA12S05-1546WT4.6×250 TA12S05-2546WT

Please inquire for 2 µm columns.

Semi-preparative columnsParticle size（µm）

Pore size（Å）


S-5 120 10×150 TA12S05-1510WT10×250 TA12S05-2510WT20×100 TA12S05-1020WT20×150 TA12S05-1520WT20×250 TA12S05-2520WT

Semi-preparative columns YMC-Actus Triart C18S-5 120 20× 50 TA12S05-0520WX

20×100 TA12S05-1020WX30× 50 TA12S05-0530WX30× 75 TA12S05-L530WX30×100 TA12S05-1030WX

See P138-141 for details of YMC-Actus series.

Guard cartridge columns (with inner diameter 2.0 mm: 2/pack, 4.0 mm: 3/pack)S-3 120 2.0×10 TA12S03-0102CC

4.0×23 TA12S03-G304CC

S-5 120 2.0×10 TA12S05-0102CC4.0×23 TA12S05-G304CC

A cartridge holder will need to be purchased separately before using this product for the first time.

Cartridge holderSemi-micro guard cartridge holder(inner diameter: 1.0, 1.5, or 2.0 mm)

XPCHSMW

Cartridge holder(inner diameter: 4.0 mm)

XPCHW

Guard columnsS-5 120 10×30 TA12S05-0310WTG

20×50 TA12S05-0520WTG

0 2 4 6 8 10 12 14 Time [min]0

1

2

3

4

5

64x10

Intens .

100ng_mL_RB4_01_2291.d: EIC 343.051±0.01 +All MS100ng_mL_RB4_01_2291.d: EIC 343.078±0.01 +All MS

Courtesy of J.Watanabe, Bruker Daltonics K. K.

1

2 Mixture 100 ppb

High resolution extracted chromatograms for［M+H］+

LC/MS analysis for Triazolam and Etizolam


Eluent ： A) 10 mM HCOOH, B) acetonitrile 25-50% B (0-10 min), 25% B (10-15 min)

Flow rate ：0.2 mL/minTemperature ：40 ℃Detection ：ESI, positive mode (Bruker Daltonics micrOTOF)Injection ：5 µL

A p p l i c a t i o n （S100121A）

Cl

N

Cl

N

NCH3

N

N

Cl

S

N

NCH3

NCH3

Triazolam (MW 343.22) Etizolam (MW 342.85)

1. 2.

第01章.indd 23 11.1.28 10:39:40 AM

24

1

Reversed-P

hase C1

8 C

olumns

（OD

S

）

Ultra-Fast LC column

Characteristics of 2 µm packing material

YMC-UltraHT series shows nearly the same efficiency as sub-2 µm columns for ultra fast LC system and reduces the column pressure significantly.

Interstitial linear velocity (mm/sec)

HE

TP (m

m/p

late

s)

0.000

0.005

0.010

0.015

0.020

0 1 2 3 4 5 6 7 8 9 10 11

Brand I7 1.7 µm

Brand L1 1.8 µm

YMC 5 µm

YMC 3 µm

YMC 2 µm

Optimum flow rate for 5 µm Optimum flow rate for 2 µm

　Van Deemter curves

(0.2 mL/min for 2 mmI.D.) (0.3-0.8 mL/min for 2 mmI.D.)

0 1 2 3 4 5 6 7 8 9 10 11

Interstitial linear velocity (mm/sec)

0

10

20

30

40

50

60

70

80

Col

umn

pre

ssur

e (M

Pa)

YMC 2 µm

YMC 5 µm

YMC 3 µm

Brand L1 1.8 µm

Brand I7 1.7 µm

　Column pressure

(0.3-0.8 mL/min for 2 mmI.D.)Optimum flow rate for 2 µm

applicable pressure range for conventional LC

Column : 50×2.0 or 2.1 mmI.D.Eluent : acetonitrile/water (60/40)Temperature : 25 ℃Sample : Butyl benzoate

YMC-UltraHT seriesPro C18 Hydrosphere C18

Particle Size (µm) 2 2Pore Size (Å) 120 120Specific Surface area (m2/g) 330 330Carbon Content 16% 12%Bonding Type Monomeric MonomericAvailable pH Range 2 - 8 2 - 8Characteristics Standard type For polar analytes

2 µm ODS column ideal for Ultra-Fast LC

Features

● Superior column performance at higher flow rate and higher pressure● Reduced analysis time while maintaining excellent resolution● Excellent resolution with back pressure less than that of sub-2 µm● Applicable to both conventional HPLC and specific ultra pressure LC system● Same selectivity and superior peak shapes as 3 µm and 5 µm Pro series● Simple method transfer from conventional HPLC on Pro series; no need to

change elution conditions

YMC-UltraHTYMC-UltraHT series is a C18 bonded-phase based on highly efficient 2 µm spherical silica particles. YMC-UltraHT series columns are specifically designed for high-speed and high throughput analysis in various fields such as pharmaceuticals, foods, and environmentals.YMC-UltraHT series has two products. Pro C18 as our

standard ODS, and Hydrosphere C18 for hydrophilic compounds. Both of them show the same selectivity and superior peak shapes as 3 µm and 5 µm Pro series, allowing for an easy method transfer from conventional HPLC to ultra-fast HPLC without changing elution conditions.

第01章.indd 24 11.1.28 10:39:43 AM

25

1

Reversed-P

hase C1

8 C

olumns

（OD

S

）

Ultra Fast analysis for flavonoids

This example shows the method transfer from conventional HPLC method to Ultra-fast LC method using a YMC-UltraHT column. Since the column efficiency is not decreased even when the flow rate is 3 times as fast as the conventional condition, it can be shorten analysis time by 90% without losing the separation characteristics.

conventional LC method ： YMC-Pack Pro C18 150×4.6 mmI.D. 5 µm

tR(7) 24.3 minN(7) 77,000 /m

90% Decrease in analysis time90% Increase in efficiency

tR(7) 2.7 minN(7) 144,000 /m

TriplelinearVeloci

tyUltra-fast LC method ： YMC-UltraHT Pro C18

50×2.0 mmI.D. 2 µm

1.0 mL/min5 µL injection8.5 MPa

0.6 mL/min1 µL injection30 MPa

Flavonoids1. Myricetin2. Quercetin3. Apigenin4. Kaempferol5. Baicalein6. Chrysin7. Acacetin

Eluent : acetonitrile/water/formic acid (35/65/0.1)Temperature : 40 ℃Detection : UV at 260 nm

Applicable for conventional LC system

Conventional LC system （8 µL, cell length 10 mm）

Conventional semi-micro LC system （2.5 µL, cell length 5 mm）

N(1) = 6300N(2) = 6300N(3) = 7500N(4) = 7800Rs(1, 2) = 2.21

N(1) = 3500N(2) = 3800N(3) = 6100N(4) = 6800Rs(1, 2) = 1.61

80

60

40

0

mAU

20

0 1 2 3 min

12

3

4

(N070606B)

0 1 2 3 min

150

100

50

0

mAU

12 3

4

(N070606E)

50×3 mmI.D.

Flow rate : 0.8 mL/minInjection : 2 µL


YMC-UltraHT series can be used for fast analysis with conventional LC system, as its pressure is lower than commercial sub-2 µm columns. Considering the extra column band broadening, the column of 3.0 mml.D. is more applicable for conventional LC system than the column of 2.0 mml.D..

Conventional LC system （8 µL, cell length 10 mm）

Conventional semi-micro LC system （2.5 µL, cell length 5 mm）

N(1) = 3700N(2) = 4100N(3) = 6100N(4) = 6900Rs(1, 2) = 1.61

N(1) = 1400N(2) = 1500N(3) = 3600N(4) = 4500Rs(1, 2) = 0.90

80

60

40

0

mAU

20

0 1 2 3 min

12 3

4

(N070608E)

0 1 2 3 min

150

100

50

0

mAU

1 23

4

(N070608B)

50×2 mmI.D.



Cephalosporin antibiotics 1. Cephalexin 2. Cefaclor 3. Cephaloglycin 4. Cephaloridine

Column ：YMC-UltraHT Pro C18Eluent ：acetonitrile/20 mM KH2PO4

　(10/90)Temperature ：37 ℃Detection ：UV at 260 nm

第01章.indd 25 11.1.28 10:39:45 AM

26

1

Reversed-P

hase C1

8 C

olumns

（OD

S

）

Analytical columns

YMC-UltraHT Pro C18● Superior column performance at higher flow rate and higher pressure● Excellent resolution with back pressure less than 60% of commercial sub-2 µm● Same selectivity and superior peak shapes as 3 µm and 5 µm Pro C18● Simple method transfer from conventional HPLC

F i r s t c h o i c e O D S c o l u m n f o r U l t r a - f a s t L CYMC-UltraHT Pro C18 is a C18 bonded-phase based on highly efficient 2 µm spherical silica particles. YMC-UltraHT Pro C18 is designed for high-speed and high throughput analysis, advanced endcapping technology

makes YMC-UltraHT Pro C18 highly appropriate for analysis of basic compounds. The physical properties and separation characteristics are strictly controlled, so it is highly suitable as the first choice column for ultra fast LC.

■Pore size：120 Å■Carbon content：16％■Usable pH range：2.0 〜 8.0

Excellent peak shapes

N(5) 106,000/mTf(5) 1.0730 MPa

12

34

5

0

60mV

40

20

0.0 1.61.20.80.4 2.0[min]

YMC-UltraHT Pro C1850×2.0 mmI.D 2 µm

Alkaloids1. Scopolamine2. Atropine3. Cinchonine4. Quinine5. Dihydroquinine

Column : 50×2.0 or 2.1 mmI.D.Eluent : 20 mM CH3COOH-CH3COONH4 (pH 4.9)/ acetonitrile (80/20)Flow rate : 0.6 mL/minTemperature : 40 ℃Detection : UV at 220 nm

sub-2 µm ODS

N(5) 89,000/mTf(5) 3.9949 MPa

12

3

45

0

60mV

40

20

0.0 1.61.20.80.4 2.0[min]

sub-2 µm ODS

N(5) 78,000/mTf(5) 2.3537 MPa

12 3

45

0

60mV

40

20

0.0 1.61.20.80.4 2.0[min]

YMC-UltraHT Pro C18 exhibits greater peak shapes of alkaloids, which are highly basic compounds compared to commercial silica based sub-2 µm columns.

Peptide mapping

0%B (0-5 min) 0-100%B (5-40 min) 100%B (40-45 min)

YMC-Pack Pro C18150×2.0 mmI.D. 5 µm

mV

80

40

0

45 min30150

(06082204.D)

＊

Eluent ：A) acetonitrile/water/TFA (10/90/0.1) 　B) acetonitrile/water/TFA (35/65/0.1)Flow rate ：0.2 mL/minTemperature ：37 ℃Detection ：UV at 220 nmSample ：tryptic degest of Cytochrome c

Analysis of a tryptic digest of cytochrome C is shown below. A 50mm length UltraHT Pro C18 column of 2 µm particle can be shorten the analysis time by one-thirds compared to a 150 mm column packed with 5 µm Pro C18.

*undigested Cytochrome c

YMC-UltraHT Pro C1850×2.0 mmI.D. 2 µm

0%B (0-1.65 min) 0-100%B (1.65-13.35 min) 100%B (13.35-15 min)

mV

200

100

0

15 min1050

（N060811I）

＊

same flow rate, column length 1/3x, gradient time 1/3x


Pore size（Å）


S-2 120 2.0× 30 AS12S02-0302WT2.0× 50 AS12S02-0502WT2.0× 75 AS12S02-L502WT2.0×100 AS12S02-1002WT3.0× 50 AS12S02-0503WT3.0× 75 AS12S02-L503WT3.0×100 AS12S02-1003WT


第01章.indd 26 11.1.28 10:40:11 AM

1

Reversed-P

hase C1

8 C

olumns

（OD

S

）

27

Analytical columns

YMC-UltraHT Hydrosphere C18● Can be used with 100% aqueous as mobile phase● Superior column performance at higher flow rate and higher pressure● Reduced analysis time while maintaining excellent resolution

T h e b e s t c o l u m n f o r h y d r o p h i l i c c o m p o u n d sYMC-UltraHT Hydrosphere C18 is a C18 bonded phase based on highly efficient 2 µm spherical silica particles.

It is designed to maintain hydrohilicity on the packing surface for superior separation of hydrophilic compounds.


Ultrafast analyses of soy isoflavones

12.5-30% B (0-10 min)1.5 mL/min3 µL injection

12.5-30% ACN (0-10 min)0.3 mL/min

12.5-30% ACN (0-3.3 min)0.9 mL/min

流速3倍、グラジエント条件最適化

(N070130I)

2 60 4 10 min8

(N060509E)

12

3

4 56 7 8

9 1011

12

2 60 4 10 min8

12

3

4 56 7

8

9 1011

12

1 20 3.3 min3

(N070130L)

1

2

3

45

6 7 8

9 10

11

12

Rs(10,11)=2.38

Rs(7,8)=2.46 Rs(10,11)=2.91

Rs(7,8)=1.75

Rs(10,11)=2.64

粒子サイズ、カラム内径をダウンサイジング

Hydrosphere C18　50×4.6 mmI.D. 3 µm

YMC-UltraHT Hydrosphere C18 2μm　50×2.0 mmI.D.

YMC-UltraHT Hydrosphere C18 2μm　50×2.0 mmI.D.

MPa

MPa

Examples illustrated shows the optimization process of isoflavones separation by 2 µm YMC-UltraHT Hydrosphere C18. It has the same selectivity as 3 µm Hydrosphere C18, so there is no need to change the condition at downsizing. Maintaining resolution, the analysis time can be reduced by using 2 µm packing material.

Soy isoflavones1. Daidzin2. Glycitin 3. Genistin4. 6"-O-Malonyldaidzin5. 6"-O-Malonylglycitin6. 6"-O-Acetyldaidzin7. 6"-O-Acetylglycitin8. 6"-O-Malonylgenistin9. Daidzein

10. Glycitein11. 6"-O-Acetylgenistin12. Genistein

12.5-30% B (0-10 min)0.3 mL/min0.6 µL injection

(N070130I)

2 60 4 10 min8

12

3

4 56 7

8

9 1011

12

Rs(10,11)=2.91

YMC-UltraHT Hydrosphere C18　50×2.0 mmI.D. 2 µm

12.5-30% B (0-3.3 min)0.9 mL/min0.6 µL injection

YMC-UltraHT Hydrosphere C18　50×2.0 mmI.D. 2 µm

1

2

3

45

6 7 8

9 10

11

12

(N070130L)

Rs(10,11)=2.64

1 20 3.3 min3

downsizing the particle size and column inner diameter

triple flow rate and optimizing the gradient condition

Eluent ：A) water/acetic acid (100/3) 　B) acetonitrile/acetic acid (100/3)Temperature ：35 ℃Detection ：UV at 254 nm


Pore size（Å）


S-2 120 2.0× 30 HS12S02-0302WT2.0× 50 HS12S02-0502WT2.0× 75 HS12S02-L502WT2.0×100 HS12S02-1002WT3.0× 50 HS12S02-0503WT3.0× 75 HS12S02-L503WT3.0×100 HS12S02-1003WT


第01章.indd 27 11.1.28 10:40:30 AM

28

1

Reversed-P

hase C1

8 C

olumns

（OD

S

）

High performance reversed-phase column

Features of Pro series ODS columnsPro series high performance reversed-phase columns feature advanced endcapping technology and quality assurance which is ready for validation. The Pro series includes three kinds of ODS, each with unique separation characteristics.

We provide products with excellent performance and stable quality all over the world to fulfill the needs of the pharmaceutical industry, in which production, as well as research and development, are increasingly globalized.

Providing products with stable quality all over the world

Feature

● Silica gel base, low in metal impurities● Processed with advanced endcapping

technology● Superior separation of basic compounds● Useful for LC/MS● Excellent durability● Excellent reproducibility● Two types of test reports are included

with each column

YMC-Pack Pro C18 RS is a high

carbon "polymeric type" ODS bonding,

characterized by high resolution and

high durability. It is applicable to a

wide range of compounds. The

separation of compounds with relatively

high hydrophobicity and those that differ

only slightly in hydrophobicity, such as

structural isomers, is outstanding. It

also has excellent resistance to acid

and alkali, making it useful under

demanding separation conditions.

High carbon ODS column, useful for separation of highly hydrophobic compounds

YMC-Pack Pro C18 RS

YMC-Pack Pro C18 is a high

performance monomeric type ODS

column providing standard

hydrophobicity, high resolution, high

durability and excellent reproducibility.

It is highly versatile and suitable as the

first choice column in almost all fields,

including pharmaceutical products,

agricultural chemicals, foods, and

natural products.

Standard ODS columnwith high versatility

YMC-Pack Pro C18

Hydrosphere C18 is an ODS column

designed to maintain maximum

hydrophilicity without activating the

silica surface. It can be used with

100% water eluent, a problematic

eluent for conventional ODS columns.

Hydrosphere C18 is useful for

separating hydrophilic compounds.

Low carbon ODS column, useful for separation of hydrophilic compounds

Hydrosphere C18

Types of Pro series ODSPro C18 Hydrosphere C18 Pro C18 RS

Particle Size (µm) 2 , 3 , 5 , 10 2 , 3 , 5 3 , 5Pore Size (Å) 120 120 80Specific Surface area (m2/g) 330 330 510Carbon Content 16% 12% 22%Bonding type monomeric monomeric polymericAvailable pH Range 2 - 8 2 - 8 1 - 10Characteristics Standard type Separation for hydrophilic compounds High durability

第01章.indd 28 11.1.28 10:40:32 AM

29

1

Reversed-P

hase C1

8 C

olumns

（OD

S

）

The three kinds of ODS columns in the Pro series each have unique carbon content and separation characteristics, including hydrophobicity, plane recognition ability, and hydrogen-bonding capacity. Selection according to the analyte and mobile phase of Pro C18 with standard hydrophobicity, Pro C18 RS with high hydrophobicity, or Hydrosphere C18 with low hydrophobicity, facilitates method development.

Three kinds of ODS, each with different separation characteristics

Hydrophobicity and plane recognition ability of Pro series ODS

5 10 15 20 250 minJ020805E

1

2

3

4

5

YMC-Pack Pro C18 RS

1. Uracil2. n-Butylbenzene3. o-Terphenyl4. n-Amylbenzene5. Triphenylene

Column size ：150×4.6 mmI.D.Eluent ：methanol/water (80/20)Flow rate ：1.0 mL/minTemperature ：37 ℃Detection ：UV at 254 nm

5 10 15 200 minJ020805B

1

2

3

4

5

YMC-Pack Pro C18

5 10 15 200 minJ020805D

1

2

3

4

5

Hydrosphere C18

Comparison of hydrophobicity and hydrogen-bonding capacity of various columns

XTerra MS C18

(S090805A)

Gemini NX-C18

2.00 4.00 6.00 8.00 10.00 12.00 14.00

Hyd

roge

n-b

ond

ing

cap

acity

α (c

affe

ine/

ben

zene

)

Hydrophobicity k’ (amylbenzene)

Inertsil ODS-4

Eluent : MeOH/W (80/20) [amylbenzene], MeOH/W (30/70) [caffeine, benzene]

Pro C18 RS

Develosil ODS-SR

Develosil C30-UG

Atlantis T3

Hydrosphere C18

CAPCELL PAK C18 AQ

Pro C18Gemini C18

Hypersil GOLD

XBridge C18Eternity-C18

Cadenza CD-C18L-column2 ODSTSKgel ODS-100S

L-column ODSMightysil RP-18

SunFireC18

Inertsil ODS-3

CAPCELL PAK C18 MGII

CAPCELL PAK C18 MGIII

Unison UK-C18

0.040

0.060

0.080

0.100

0.120

0.140

0.160

0.180

Hydrophobicity: lowHydrogen-bonding capacity: high

Hydrophobicity: intermediateHydrogen-bonding capacity: middle

Hydrophobicity: highHydrogen-bonding capacity: low

第01章.indd 29 11.1.28 10:40:35 AM

30

1

Reversed-P

hase C1

8 C

olumns

（OD

S

）

High performance reversed-phase column

Features of Pro series ODS columns

Comparison of separation behavior on the three kinds of ODS columns in the Pro series

Column ：150×4.6 mmI.D.Eluent ：methanol/water (55/45)Flow rate ：1.0 mL/minTemperature ：30 ℃Detection ：UV at 270 nm

Parabenzoates

3 types of ODS are available to separate the para-benzoates shown in the left, but the best separation between peaks 5 and 6 is achieved on Pro C18 RS.

1

2

3 4

65

10 20 300 min（J020827E）

Pro C18 RS

1

2

3 4

65

10 200 min（J020827D）

Pro C18

1

2

3 4

65

10 200 min（J020827C）

Hydrosphere C18

Methyl p-hydroxybenzoate

OH

COOCH3

Ethyl p-hydroxybenzoate

Propyl p-hydroxybenzoate

Butyl p-hydroxybenzoate

Isopropyl p-hydroxybenzoate

Isobutyl p-hydroxybenzoate

COOCH

CH3

CH3

OH

OH

COOCH2CH3

COO(CH2)2CH3

OH

COOCH2CH

CH3

CH3

OHCOO(CH2)3CH3

OH

1.

2.

3.

4.

5.

6.

Flavonoid glycosides

Pro C18 RS

Pro C18

Hydrosphere C18

5 10 150 min

1

2

3

J020828F

4

1

2

3

4

J020828A

1

2

3

4

J020828C

O

O

HO

OH

HO

OR

HO

Hydrogen-bonding interactions significantly contribute to the retention of hydrophilic compounds, including glycosides. Therefore, the retention is longer in the Hydrosphere C18, which has strong hydrogen-bonding capacity, than in Pro C18 RS, which has highly hydrophobic packing materials. To separate such hydrophilic compounds, the Hydrosphere C18 is suitable for precise separation of multicomponent samples, while Pro C18 RS and Pro C18 are suitable for rapid separation of simple components.

Column ：150×4.6 mmI.D.Eluent ：acetonitrile/10 mM H3PO4 (25/75)Flow rate ：1.0 mL/minTemperature ：40 ℃Detection ：UV at 260 nm

1. Rutin2. Isoquercitrin3. Quercitrin4. Quercetin

R = rutinoseR = glucoseR = rhamnoseR = H

第01章.indd 30 11.1.28 10:40:39 AM

31

1

Reversed-P

hase C1

8 C

olumns

（OD

S

）

Terphenyl isomers

1

2

3

10 200 minJ020719E

Pro C18 RS

1.

2.

3.

m-Terphenyl

o-Terphenyl

p-Terphenyl

Since Pro C18 RS has excellent hydrophobic selectivity and shape selectivity, it works well in the separation of structural isomers that are not easily separated by other ODS.

Column ：150×4.6 mmI.D.Eluent ：methanol/water 　(85/15)Flow rate ：1.0 mL/minTemperature ：37 ℃Detection ：UV at 254 nm

1

2

3

10 200 minJ020719J

Pro C18

1

2

3

10 200 minJ020722A

Hydrosphere C18

10 200 minJ020719K

Brand C1

1

2,3

Saikosaponins

Pro C18

Hydrosphere C18

Pro C18 RS

Brand B2

10 20 30 400

F030224E

1

23

4

4

4

1

1

2,3

2,3

F030222C

F030222A

min

1

23

4

F030222B

Peaks 2 and 3, which are not easily separated by other ODS, can be completely separated by Hydrosphere C18. It is thought that hydrogen-bonding interaction of hydrophilic functional groups in sugar chain and terpene skeleton as well as hydrophobic interaction has great contribution to the separation of saikosaponins.Hydrosphere C18 is the most appropriate in the separation of this kind of class of compound.

O

glucose glucose64rhamnose

O

H

OH

Saikosaponin c

O

fucose glucose3

O

H

OH

CH2OH

Saikosaponin a

Saikosaponin b2

O

fucose glucose3

O

H

OH

CH2OH

Saikosaponin d

1.

2.

3.

4.

fucose glucose3

O

OH

CH2OH

CH2OH

Column ：150×4.6 mmI.D.Eluent ：acetonitrile/water (38/62)Flow rate ：1.0 mL/minTemperature ：37 ℃Detection ：UV at 210 nm

第01章.indd 31 11.1.28 10:40:44 AM

32

1

Reversed-P

hase C1

8 C

olumns

（OD

S

）

Analytical columns

YMC-Pack Pro C18● Superior separation of basic

compounds● Processed with advanced

endcapping technology

● Excellent reproducibility● Utilizes highly pure silica gel base● Two types of test reports are included

with each column

P e r f e c t l y e n d c a p p e d O D SYMC-Pack Pro C18 includes more advanced endcapping technology for strictly controlled processing of residual silanol groups that are likely to affect quality. Pro C18 is highly appropriate for basic compounds, including pharmaceutical products and agricultural chemicals.

Two test reports are included with each product; a Column Test Report, indicating the column performance, and a Packing Material Test Report, detailing strictly controlled physical properties and separation characteristics of the packing material.


Useful for separation of basic compounds

Column ：YMC-Pack Pro C18 (5 µm, 120 Å) 　150×4.6 mmI.D.Eluent ：20 mM KH2PO4-K2HPO4 (pH6.9)/ 　methanol (25/75)Flow rate ：1.0 mL/minTemperature ：37 ℃Detection ：UV at 254 nm

Tricyclic antidepressants

（A961015G）0 10 20 min

1

2 3

4Advanced endcapping technology results in good peak shapes even for basic compounds that often elute with poor tailing shapes on competitive columns.

CHCH2CH2NCH3

CH3

HCI・N

CH2CH2CH2N

CH2CH2CH2NHCH3

CH3

CH3

HCI・

NHCI・

1.

Desipramine hydrochloride

2.

Acenaphthene(I.S.)

3.

Imipramine hydrochloride

4.

Amitriptyline hydrochloride

Basic peptides

（A990123B）

1

23

4

56

0 5 10 15 20 min

Pro C18YMC-Pack Pro C18 is processed using advanced endcapping technology and produces a better chromatogram than that obtained using ODS-A for separating basic peptides.Similar chromatograms have been obtained with YMC-Pack Pro C18 and ODS-A for separation of neutral peptides.

（A990121A）

1

2

3

4

56

0 5 10 15 20 min

ODS-A

Neutral peptides

（A990121B）0 5 10 15 20 min

1

2

3 45

6

Pro C18

（A990119B）0 5 10 15 20 min

1

2

34

56

ODS-A

1. Bradykinin2. Dynorphin A (Human,1-13)3. Eledoisin related peptide4. Neurotensin5. Bombesin6. Substance P

Column ：YMC-Pack Pro C18 and ODS-A (5 µm, 120 Å) 　75×4.6 mmI.D.Eluent ：A) water/TFA (100/0.1) 　B) acetonitrile/TFA (100/0.1) 　20-40％ B (0-20 min,linear)Flow rate ：1.0 mL/minTemperature ：37 ℃Detection ：UV at 220 nm

1. Oxytocin2. Met-Enkephalin3. Leu-Enkephalin4. Angiotensin l5. α-Mating factor6. Insulin

第01章.indd 32 11.1.28 10:40:48 AM

33

1

Reversed-P

hase C1

8 C

olumns

（OD

S

）

Excellent lot-to-lot reproducibilitybatch-to-batch

0 4 8 min

Lot C

Lot B

Lot A

Test B

1. Caffeine2. Pyridine3. Phenol

A three-lot comparison is shown on the left.Excellent reproducibility is achieved in separating hydrophilic, basic and acidic compounds, as well as hydrophobic compounds.

0 5 10 15 min

Lot C

Lot B

Lot A

Test A

1. Uracil2. 4-n-Butoxybenzoic acid3. Ethylbenzene4. n-Propylbenzene

column-to-column

Advanced packing technology results in high column efficiency. All packed columns are inspected and controlled according to the strictest quality control criteria for column performance ever, including theoretical plate number testing.

24000

21000

18000

15000

12000

9000

6000

300020 40 60 80 100

Column No.

Theoretical plate number

150×4.6 mml.D. theoretical plate number

Two types of test reports〈Packing Material Test Report〉

Each YMC-Pack Pro C18 includes both, a Column Test Report and a Packing Material Test Report, to certify lot-to-lot and column-to-column reproducibility.

〈Column Test Report〉

This report describes basic physical properties of silica gel, including particle size, pore size and metal content, carbon loading after modification, and the specifications and results of separation characteristics tests.

The Column Test Report shows the theoretical plate number and asymmetry factor to indicate column performance. For convenience in deterioration evaluation, this report includes all analytical conditions including sample concentration as well.

第01章.indd 33 11.1.28 10:40:51 AM

34

1

Reversed-P

hase C1

8 C

olumns

（OD

S

）

Analytical columns

YMC-Pack Pro C18

1

2

3 4 5

6

min0 5 10 15 20

F040119B

Pro C18

Column ：150×4.6 mmI.D.Eluent ：A) 20 mM KH2PO4-H3PO4 (pH 2.5) 　B) methanol 　20-90% B (0-15 min), 90% B (15-20 min)Flow rate ：1.0 mL/minTemperature ：37 ℃Detection ：UV at 260 nm

1 2

3 4 5

6

min0 5 10 15 20

F030130C

Brand I4

min0 5 10 15 20

F040130E

1

2

34, 5

6

Brand L1

Naphazoline Chlorpheniramine

Methyl p-hydroxybenzoate Benzethonium chloride

LidocaineMaleic acid

1 2 3 4

5 6

CHCOOHCHCOOH CH3)2N(CH2NHCCH2

OCH3

CH3 CH2

N

N

HCHCH2CH2N

CH3CH3

N

Cl

OH

COOCH3 CH2N

CH3

CH3

(OCH2CH2)2 Cl-+C

CH3

CH3

CH2C

CH3

CH3

CH3

nasal drops (OTC)

2

3 4

5

1

0 10 20 min

catechins1. (-)-Epigallocatechin2. (+)-Catechin3. (-)-Epicatechin4. (-)-Epigallocatechin gallate5. (-)-Epicatechin gallate

Column : YMC-Pack Pro C18 (5 µm, 120 Å) 150×4.6 mmI.D.Eluent : acetonitrile/ethyl acetate/water/TFA (9/3/88/0.1)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 280 nm

12

3

4

6 7

5

0 5 10 15 min

antioxidants1. n-Propyl gallate(PG)2. 2,4,5-Trihydroxybutyrophenone (THBP)3. tert-Butylhydroquinone(TBHQ)4. Nordihydroguaiaretic acid (NDGA)5. 4-Hydroxymethyl-2,6-di-tert-butylphenol (HMBP)6. n-Octyl gallate(OG)7. n-Dodecyl gallate(DG)

Column ：YMC-Pack Pro C18 (5 µm, 120 Å) 　75×4.6 mm I.D.Eluent ：A) acetonitrile/methanol/5% acetic acid (20/20/60) 　B) acetonitrile/methanol/5% acetic acid (45/45/10) 　0-100% B (0-5 min), 100% B (5-15 min)Flow rate ：1.0 mL/minTemperature ：30 ℃Detection ：UV at 280 nm

A p p l i c a t i o n

A p p l i c a t i o n （N050620A）

A p p l i c a t i o n （A970526C）

第01章.indd 34 11.1.28 10:40:55 AM

35

1

Reversed-P

hase C1

8 C

olumns

（OD

S

）


Pore size（Å）


S-3 120 1.0× 35 AS12S03-H501WT1.0× 50 AS12S03-0501WT1.0×100 AS12S03-1001WT1.5× 35 AS12S03-H5P5WT1.5× 50 AS12S03-05P5WT1.5×100 AS12S03-10P5WT2.0× 35 AS12S03-H502WT2.0× 50 AS12S03-0502WT2.0× 75 AS12S03-L502WT2.0×100 AS12S03-1002WT2.0×150 AS12S03-1502WT3.0× 50 AS12S03-0503WT3.0×100 AS12S03-1003WT3.0×150 AS12S03-1503WT4.6× 35 AS12S03-H546WT4.6× 50 AS12S03-0546WT4.6× 75 AS12S03-L546WT4.6×100 AS12S03-1046WT4.6×150 AS12S03-1546WT6.0× 50 AS12S03-0506WT6.0× 75 AS12S03-L506WT6.0×100 AS12S03-1006WT

S-5 120 1.0×150 AS12S05-1501WT1.0×250 AS12S05-2501WT1.5×150 AS12S05-15P5WT1.5×250 AS12S05-25P5WT2.0× 35 AS12S05-H502WT2.0× 50 AS12S05-0502WT2.0× 75 AS12S05-L502WT2.0×100 AS12S05-1002WT2.0×150 AS12S05-1502WT2.0×250 AS12S05-2502WT3.0× 50 AS12S05-0503WT3.0× 75 AS12S05-L503WT3.0×100 AS12S05-1003WT3.0×150 AS12S05-1503WT3.0×250 AS12S05-2503WT4.6× 50 AS12S05-0546WT4.6× 75 AS12S05-L546WT4.6×100 AS12S05-1046WT4.6×150 AS12S05-1546WT4.6×250 AS12S05-2546WT6.0×150 AS12S05-1506WT6.0×250 AS12S05-2506WT

S-10 120 4.6×150 AS12S11-1546WT4.6×250 AS12S11-2546WT


Pore size（Å）


S-5 120 10×150 AS12S05-1510WT10×250 AS12S05-2510WT20×100 AS12S05-1020WT20×150 AS12S05-1520WT20×250 AS12S05-2520WT

S-10 120 20×100 AS12S11-1020WT20×150 AS12S11-1520WT20×250 AS12S11-2520WT

See P142 for preparative columns other than those listed above.

Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)S-3 120 1.0×10 AS12S03-0101CC

1.5×10 AS12S03-01P5CC2.0×10 AS12S03-0102CC4.0×23 AS12S03-G304CC

S-5 120 1.0×10 AS12S05-0101CC1.5×10 AS12S05-01P5CC2.0×10 AS12S05-0102CC4.0×23 AS12S05-G304CC

A guard cartridge holder will need to be purchased separately before using this product for the first time.


XPCHSMW


XPCHW

YMC guard cartridge packS-3 120 4.0×23 AS12S03-G304CCPK

S-5 120 4.0×23 AS12S05-G304CCPK

A guard cartridge pack includes a cartridge holder (1 set), a guard cartridge column, and a column coupler (produced by PEEK).

Guard columnsS-5 120 10×30 AS12S05-0310WTG

20×50 AS12S05-0520WTG

S-10 120 20×50 AS12S11-0520WTG

When columns packed with material from different lots are needed for validation purposes, please contact us.


第01章.indd 35 11.1.28 10:40:56 AM

36

1

Reversed-P

hase C1

8 C

olumns

（OD

S

）

Analytical columns

Hydrosphere C18● Strong retention of hydrophilic compounds● Can be used with 100% water mobile phase● Superior separation of basic compounds● Excellent reproducibility

● Utilizes highly pure silica gel base● Two types of test reports are included with

each column

H y d r o p h i l i c O D SHydrosphere C18 is designed to maintain adequate hydrophilicity on the packing surface for superior separation of hydrophilic compounds. Hydrophilic compounds are retained with much greater affinity than with conventional ODS columns, permitting the product to be used

with 100% water eluent, a problematic eluent for conventional ODS columns. Hydrosphere C18 is useful for separating a wide range of compounds, including nucleic acids and their derivatives, organic acids, saccharides, glycosides and peptides.


Appropriate for separation of hydrophilic compoundsSeparation of organic acids

0 5 10 minA000310E

1

2

34

5

Hydrosphere C18High hydrophilicity of the Hydrosphere C18 packing material surface enables longer retention and superior separation of citric acid and fumaric acid as compared to a conventional ODS column (Brand Ⅰ).

1

2

3,4

5

5 100 minA000615A

conventional ODS column （Brand Ⅰ）

1. Lactic acid2. Acetic acid3. Citric acid4. Fumaric acid5. Succinic acid

Column ：150×4.6 mm I.D.Eluent ：20 mM NaH2PO4-H3PO4 (pH 2.8)Flow rate ：1.0 mL/minTemperature ：30 ℃Detection ：UV at 210 nm

Reproducibility of retention time when used with 100% water mobile phase

S0515A10 200 min

※

21

3

4 5

Hydrosphere C18When conventional ODS columns are used with 100% water mobile phase, the apparent hydrophobicity decreases due to repulsive interactions between water and hydrophobic groups on the surface of the packing materials, reducing retention of compounds. In the experiment illustrated here, columns filled with 100% water mobile phase were left to stand overnight. Retention times of nucleic acid bases were analyzed before and after standing and compared. The retention times of adenine after standing decreased to 89% of the original value for Brand II and to 71% of the original Brand III value. By contrast, Hydrosphere C18 retained 98% of its original retention for adenine. Hydrosphere C18 is designed to maintain adequate hydrophilicity on the packing surface so that hydration can be achieved, and when Hydrosphere C18 is used with mobile phase containing no organic solvent, the retention time is not significantly shortened and highly reproducible chromatograms can be obtained.

S0518H10 200 min

※

2

1

3

4 5

conventional ODS column （Brand Ⅱ）

S0518F10 200 min

※

21

3

45

conventional ODS column （Brand Ⅲ）

S0517A

※ 98％

10 200 min

89％

S0519A

※

10 200 min

71％

S0519C

※

10 200 min

1. Cytosine2. Uracil3. Guanine4. Thymine5. Adenine

Column ：150×4.6 mm I.D.Eluent ：20 mM KH2PO4-K2HPO4 (pH 6.9)Flow rate ：1.0 mL/minTemperature ：37 ℃Detection ：UV at 254 nm

Afte

r sta

ndin

gBe

fore

sta

ndin

g

After completion of analysis, the flow was stopped and the columns were left to stand overnight (ca. 15 hours).

第01章.indd 36 11.1.28 10:41:01 AM

37

1

Reversed-P

hase C1

8 C

olumns

（OD

S

）

Differences in separation characteristics from conventional ODS columns

0 10 20 30 40 minS000309A

1

2

34

5 6

Hydrosphere C18The chromatograms shown were obtained in tests conducted to compare separation characteristics using a mixed sample containing compounds with different characteristics. The packing material surface of Hydrosphere C18 has higher hydrophilicity and lower C18 ligand density than those of Pro C18. This results in shorter retention of hydrophobic compounds, including n-propylbenzene and n-butylbenzene, and planar compounds like acenaphthene. By contrast, retention of testosterone, a compound with large hydrogen-bonding properties, is longer on Hydrosphere C18 and on Brand IV, which also has a highly hydrophilic packing surface.Amitriptyline, a basic compound, caused tailing peaks in Brand IV and Brand V. By contrast, Hydrosphere C18 and Pro C18 produce good peak shapes for such basic compounds. Although the retention times of amitriptyline on Hydrosphere C18 and Pro 18 are similar, there is a big difference between the two columns with regard to resolution of n-butylbenzene and amitriptyline. This difference is considered to be due to the hydrophilicity of the Hydrosphere C18 surface.These results demonstrate that Hydrosphere C18, having different separation characteristics from conventional ODS columns, enables separation of compounds that cannot be separated using other ODS columns.0 10 20 30 40 min

12

34

5 6

S990607B

Pro C18

10 20 30 40 50 600 minF000614B

12

34

5

6

Brand Ⅳ

10 20 30 40 50 60 70 80 900 minF000614C

12

3 4 5

6

Brand Ⅴ

1. Toluene2. Testosterone3. n-Propylbenzene4. Acenaphthene5. n-Butylbenzene6. Amitriptyline hydrochloride

Column ：150×4.6 mm I.D.Eluent ：20 mM KH2PO4-K2HPO4 (pH 6.9)/methanol (35/65)Flow rate ：1.0 mL/minTemperature ：37 ℃Detection ：UV at 254 nm

第01章.indd 37 11.1.28 10:41:04 AM

38

1

Reversed-P

hase C1

8 C

olumns

（OD

S

）

Analytical columns

Hydrosphere C18

5 10 15 20 250 min

1 2

3

4

5

6

Nicotinic acid and derivatives1. Nicotinamide adenine dinucleotide phosphate oxidized form (NADP)2. Nicotinic acid3. Nicotinamide adenine dinucleotide phosphate reduced form (NADPH)4. Nicotinamide5. β-Nicotinamide adenine dinucleotide (NAD)6. β-Nicotinamide adenine dinucleotide reduced form (NADH)

Column ：Hydrosphere C18 (5 µm, 120 Å) 　150×4.6 mm I.D.Eluent ：20 mM KH2PO4-K2HPO4 (pH 6.9)/acetonitrile (99/1)Flow rate ：1.0 mL/minTemperature ：37 ℃Detection ：UV at 260 nm　

A p p l i c a t i o n （F000707B）

Short analysis time

Column ：50×4.6 mm I.D. (3 µm, 120 Å)Eluent ：acetonitrile/methanol/5% acetic acid (35/35/30)Flow rate ：1.0 mL/minTemperature ：30 ℃Detection ：UV at 280 nm

Hydrosphere C18 can reduce the time necessary for analyzing compounds with high hydrophobicity by about 20% as compared with conventional ODS columns.

5 10 150 minJ010216B

1

3

4

5

2

6

conventional ODS column

5 10 150 minJ010215B

1

3

4 52 6

Hydrosphere C18

Antioxidants1. Nordihydroguaiaretic acid (NDGA)2. Butyl hydroxyanisol (BHA)3. 4-Hydroxy-2,6-di-tert-butylphenol (HMBP)4. n-Octyl gallate (OG)5. Dibutyl hydroxytoluene (BHT)6. n-Dodesyl gallate (DG)

1

2

3

4

6

7

5

5 10 15 200 min

8

911

10

Nucleotides1. 5’-UTP 7. 5’-ATP2. 5’-UDP 8. 5’-IMP3. 5’-CMP 9. 5’-ADP4. 5’-UMP 10. 5’-TMP5. 5’-dCMP 11. 5’-AMP6. 5’-GMP

Column ：Hydrosphere C18 (5 µm, 120 Å) 　150×4.6 mm I.D.Eluent ：100 mM KH2PO4-K2HPO4 (pH 5.5)Flow rate ：1.0 mL/minTemperature ：30 ℃Detection ：UV at 260 nm

A p p l i c a t i o n （A000922A）

第01章.indd 38 11.1.28 10:41:07 AM

39

1

Reversed-P

hase C1

8 C

olumns

（OD

S

）


Pore size（Å）


S-3 120 1.0× 35 HS12S03-H501WT1.0× 50 HS12S03-0501WT1.0×100 HS12S03-1001WT1.5× 35 HS12S03-H5P5WT1.5× 50 HS12S03-05P5WT1.5×100 HS12S03-10P5WT2.0× 35 HS12S03-H502WT2.0× 50 HS12S03-0502WT2.0× 75 HS12S03-L502WT2.0×100 HS12S03-1002WT2.0×150 HS12S03-1502WT3.0× 50 HS12S03-0503WT3.0×100 HS12S03-1003WT3.0×150 HS12S03-1503WT4.6× 35 HS12S03-H546WT4.6× 50 HS12S03-0546WT4.6× 75 HS12S03-L546WT4.6×100 HS12S03-1046WT4.6×150 HS12S03-1546WT6.0× 50 HS12S03-0506WT6.0× 75 HS12S03-L506WT6.0×100 HS12S03-1006WT

S-5 120 1.0×150 HS12S05-1501WT1.0×250 HS12S05-2501WT1.5×150 HS12S05-15P5WT1.5×250 HS12S05-25P5WT2.0× 35 HS12S05-H502WT2.0× 50 HS12S05-0502WT2.0× 75 HS12S05-L502WT2.0×100 HS12S05-1002WT2.0×150 HS12S05-1502WT2.0×250 HS12S05-2502WT3.0× 50 HS12S05-0503WT3.0× 75 HS12S05-L503WT3.0×100 HS12S05-1003WT3.0×150 HS12S05-1503WT3.0×250 HS12S05-2503WT4.6× 50 HS12S05-0546WT4.6× 75 HS12S05-L546WT4.6×100 HS12S05-1046WT4.6×150 HS12S05-1546WT4.6×250 HS12S05-2546WT6.0×150 HS12S05-1506WT6.0×250 HS12S05-2506WT


Pore size（Å）


S-5 120 10×150 HS12S05-1510WT10×250 HS12S05-2510WT20×100 HS12S05-1020WT20×150 HS12S05-1520WT20×250 HS12S05-2520WT

Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)S-3 120 1.0×10 HS12S03-0101CC

1.5×10 HS12S03-01P5CC2.0×10 HS12S03-0102CC4.0×23 HS12S03-G304CC

S-5 120 1.0×10 HS12S05-0101CC1.5×10 HS12S05-01P5CC2.0×10 HS12S05-0102CC4.0×23 HS12S05-G304CC



XPCHSMW


XPCHW

YMC guard cartridge packS-3 120 4.0×23 HS12S03-G304CCPK

S-5 120 4.0×23 HS12S05-G304CCPK


Guard columnsS-5 120 10×30 HS12S05-0310WTG

20×50 HS12S05-0520WTG


第01章.indd 39 11.1.28 10:41:08 AM

40

1

Reversed-P

hase C1

8 C

olumns

（OD

S

）

Analytical columns

YMC-Pack Pro C18 RS● Excellent acid resistance and alkali

resistance (pH 1 to 10)● Superior separation of structural isomers● Superior separation of basic compounds

● Excellent reproducibility● Utilizes highly pure silica gel base● Two types of test reports are included

with each column

H i g h l y d u r a b l e O D SYMC-Pack Pro C18 RS is a polymeric type high carbon ODS column characterized by high resolution and high durability. It is applicable to a wide range of compounds, providing good separation of basic compounds that easily cause

tailing peaks. The separation selectivity for compounds that differ only slightly in hydrophobicity is outstanding. It also has excellent resistance to acid and alkali, making it useful under demanding separation conditions.


Excellent durability

0

20

40

60

80

100

0 20 40 60 80 100(hours)

YMC-Pack Pro C18 RSAcid-resistant column(manufactured by other company)

Monomeric ODS

Hours exposure to acetonitrile/water/TFA (10/90/1), change in packing material hydrophobicity at 70 ℃.

%

Retention

of

butyl

benzoate

The Pro C18 RS packing material hydrophobicity is hardly changed under the harsh conditions of pH1 and column temperature 70 ℃.

pH 1

0

20

40

60

80

100

0 200 400 600 800(hours)

Pro C18 RSMonomeric ODS

Sequential analysis using 20 mM H3BO3-NaOH (pH 9.8)/methanol (50/50)

%

Theoretical

Plate

Number (

N)

of

methyl

benzoate.

Unlike conventional ODS columns, Pro C18 RS can be used with an alkali mobile phase.

pH 10

Pro C18 RS with excellent acid and alkali resistance can be used over a wide pH range, and it is easy to optimize the separating conditions. In addition, by downsizing from the column (particle 5 µm, length 150 mm) to a shorter column (particle 3 µm, length 50 mm), with high separation ability, it becomes possible to shorten the analysis time by one third without affecting the separation.

1. Flurbiprofen2. Methyl benzoate3. Lidocaine4. Diltiazem5. Verapamil

Column ：YMC-Pack Pro C18 RSEluent ：20 mM acetate buffer/methanol (35/65)Flow rate ：1.0 mL/minTemperature ：30 ℃Detection ：UV at 220 nm

A wide choice of mobile phases facilitates method development

0 5 10 15 20 min 0 5 10 15 20 min F040714EF040716A

3,4,5

1

2 21

3

5

4

0 5 10 15 20 minF040714C

21

3

5

4

150×4.6 mmI.D. 5 µm

pH 3.2 pH 6.8 pH 8.8

0 5 10 minF040714D

21 3

5

4

50×4.6 mmI.D. 3 µm

pH 8.8

Downsizing

第01章.indd 40 11.1.28 10:41:12 AM

41

1

Reversed-P

hase C1

8 C

olumns

（OD

S

）

Separation of basic compounds

Amitriptyline is a basic compound that can easily tail on some silica based columns. In comparison with competing acid-resistant columns, Pro C18 RS produces good peak shapes without tailing.

Tf(4) 1.16

10 200 minJ011114B

1

2

3 4

YMC-Pack Pro C18 RS

Tf(4) 2.87

Acid-resistant column manufacturedby other company（Brand D3）

1

2

34

10 200 minJ020523D

1. Desipramine 2. Diphenyl (I.S.)3. Imipramine 4. Amitriptyline

Column ：150×4.6 mmI.D.Eluent ：20 mM KH2PO4-K2HPO4 (pH 6.9) /methanol (25/75)Flow rate ：1.0 mL/minTemperature ：37 ℃Detection ：UV at 254 nm

Separation of structural isomers

Pro C18 RS with a high C18 density is excellent in hydrophobicity recognition ability and plane recognition ability, and can be applied to separate structural isomers which are difficult to separate with other ODS. In addition, by using 3 µm column packing, resolution can be increased, and it becomes possible to achieve a baseline separation of linolenate isomers.

F040713D0 10 20 30 min

2

1

Brand F1(standard-type ODS 5 µm)

Rs=0.8

F0407140 10 20 30 min

2

1

Rs=1.24

Pro C18 RS5 µm

Methyl linolenate isomers1. Methyl linolenate2. Methyl γ-linolenate

Column ：150×4.6 mmI.D.Eluent ：acetonitrile/THF/water (35/30/35)Flow rate ：1.0 mL/minTemperature ：30 ℃Detection ：UV at 210 nm

F040713A0 10 20 30 min

2

1

Pro C18 RS3 µm

Rs=1.54

第01章.indd 41 11.1.28 10:41:15 AM

42

1

Reversed-P

hase C1

8 C

olumns

（OD

S

）

Analytical columns

YMC-Pack Pro C18 RS

Separation of peptides by LC/MS

400 600 800 1000 1200(m/z)0

100

%

530.95

531.47

1060.27

5.00 10.00 15.00 20.00（Time）18

100

% 1

2 3

4

5 6

Bradykinin(MW: 1059.56)M+2

M+1

Pro C18 RS produces good peak shapes when it is used with mobile phase containing low TFA concentration, making it suitable for LC/MS.

1. [Arg8]-Vasopressin2. Bradykinin3. Angiotensin Ⅱ4. Oxytocin5. Angiotensin Ⅰ6. Leu-Enkephalin

Column ：YMC-Pack Pro C18 RS (5 µm, 80 Å) 　150×2.0 mmI.D.Eluent ：A) water/TFA (100/0.01) 　B) acetonitrile/TFA (100/0.01) 　10-35% B (0-15 min)Flow rate ：0.2 mL/minTemperature ：37 ℃Detection ：ESI positive-mode

1

2

3

10 200 minJ011026B

Column ：YMC-Pack Pro C18 RS (5 µm, 80 Å) 　150×4.6 mmI.D.Eluent ： acetonitrile/water/acetic acid

(30/70/1)Flow rate ：1.0 mL/minTemperature ：37 ℃Detection ：UV at 230 nm

Chloroacetophenone isomers

10 200 minJ011113D

1

2

3

Chlorophenol isomers

Column ：YMC-Pack Pro C18 RS (5 µm, 80 Å) 　150×4.6 mmI.D.Eluent ：acetonitrile/water (40/60)Flow rate ：1.0 mL/minTemperature ：30 ℃Detection ：UV at 254 nm

1.

2.

3.

m-Chloroacetophenone

COCH3

Cl

o-Chloroacetophenone

COCH3Cl

COCH3

Clp-Chloroacetophenone

1.

2.

3.

m-Chlorophenol

o-Chlorophenol

p-Chlorophenol

OH

Cl

OHCl

OH

Cl

A p p l i c a t i o n

第01章.indd 42 11.1.28 10:41:19 AM

43

1

Reversed-P

hase C1

8 C

olumns

（OD

S

）


Pore size（Å）


S-3 80 1.0× 35 RS08S03-H501WT1.0× 50 RS08S03-0501WT1.0×100 RS08S03-1001WT1.5× 35 RS08S03-H5P5WT1.5× 50 RS08S03-05P5WT1.5×100 RS08S03-10P5WT2.0× 35 RS08S03-H502WT2.0× 50 RS08S03-0502WT2.0× 75 RS08S03-L502WT2.0×100 RS08S03-1002WT2.0×150 RS08S03-1502WT3.0× 50 RS08S03-0503WT3.0×100 RS08S03-1003WT3.0×150 RS08S03-1503WT4.6× 35 RS08S03-H546WT4.6× 50 RS08S03-0546WT4.6× 75 RS08S03-L546WT4.6×100 RS08S03-1046WT4.6×150 RS08S03-1546WT6.0× 50 RS08S03-0506WT6.0× 75 RS08S03-L506WT6.0×100 RS08S03-1006WT

S-5 80 1.0×150 RS08S05-1501WT1.0×250 RS08S05-2501WT1.5×150 RS08S05-15P5WT1.5×250 RS08S05-25P5WT2.0× 35 RS08S05-H502WT2.0× 50 RS08S05-0502WT2.0× 75 RS08S05-L502WT2.0×100 RS08S05-1002WT2.0×150 RS08S05-1502WT2.0×250 RS08S05-2502WT3.0× 50 RS08S05-0503WT3.0× 75 RS08S05-L503WT3.0×100 RS08S05-1003WT3.0×150 RS08S05-1503WT3.0×250 RS08S05-2503WT4.6× 50 RS08S05-0546WT4.6× 75 RS08S05-L546WT4.6×100 RS08S05-1046WT4.6×150 RS08S05-1546WT4.6×250 RS08S05-2546WT6.0×150 RS08S05-1506WT6.0×250 RS08S05-2506WT


Pore size（Å）


S-5 80 10×150 RS08S05-1510WT10×250 RS08S05-2510WT20×100 RS08S05-1020WT20×150 RS08S05-1520WT20×250 RS08S05-2520WT

Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)S-3 80 1.0×10 RS08S03-0101CC

1.5×10 RS08S03-01P5CC2.0×10 RS08S03-0102CC4.0×23 RS08S03-G304CC

S-5 80 1.0×10 RS08S05-0101CC1.5×10 RS08S05-01P5CC2.0×10 RS08S05-0102CC4.0×23 RS08S05-G304CC



XPCHSMW


XPCHW

YMC guard cartridge packS-3 80 4.0×23 RS08S03-G304CCPK

S-5 80 4.0×23 RS08S05-G304CCPK


Guard columnsS-5 80 10×30 RS08S05-0310WTG

20×50 RS08S05-0520WTG


第01章.indd 43 11.1.28 10:41:20 AM

44

1

Reversed-P

hase C1

8 C

olumns

（OD

S

）

Analytical columns

YMC-Pack ODS-A● Conventional ODS column● Currently in use worldwide

S t a n d a r d O D SYMC-Pack ODS-A has a highly endcapped surface structure and appropriate hydrophobicity for separation of a wide range of compounds. It is produced under strict quality control with respect to 50 or more parameters in

order to ensure stable quality.This product is highly regarded as the standard YMC-Pack packing material in various countries around the world.

■Pore size：120 Å, 200 Å, 300 Å■Carbon content：17％ (120 Å), 12％ (200 Å), 7％ (300 Å)■Usable pH range：2.0 〜 7.5

Quality control system for excellent performance

10

8

6

4

2

01 2 3 4 5 6 7 8 9 10

tR (B

utyl

ben

zoat

e), m

in

Lot Number

The graph indicates lot-to-lot reproducibility with respect to hydrophobic interaction for 10 lots, an essential column performance characteristic. Extremely stable lot-to-lot reproducibility of the hydrophobic surface is achieved by strict control of variables.In addition to measurement of the physical properties, a wide range of compounds including hydrophobic, acidic, basic and coordination compounds are analyzed under optimal conditions to evaluate column performance.

3

2

1

4

3020100 min

Analgesics

Column ：YMC-Pack ODS-A 　(5 µm, 120 Å) 　150×4.6 mmI.D.Eluent ：methanol/water (40/60)Flow rate ：0.7 mL/minTemperature ：30 ℃Detection ：UV at 220 nm

3CH

Acetaminophen

1 OHCOHN

3CH

3H C

O

O

3CH

Caffeine

2N

N

N

N

2CH

3H C

3H C

2

2

CHCH=

Apronalide

42CONH

2 5OC H

Ethenzamide

3CHCHCONHCONH

2

1

20100 min

Carotenes1.α-Carotene2.β-Carotene

Column ：YMC-Pack ODS-A 　(5 µm, 120 Å) 　150×4.6 mmI.D.Eluent ：acetonitrile/methanol/THF 　(50/45/5)Flow rate ：1.0 mL/minTemperature ：30 ℃Detection ：UV at 450 nm


Pore size（Å）


S-3 120 2.0× 35 AA12S03-H502WT2.0× 50 AA12S03-0502WT2.0× 75 AA12S03-L502WT2.0×100 AA12S03-1002WT2.0×150 AA12S03-1502WT3.0× 50 AA12S03-0503WT3.0×100 AA12S03-1003WT3.0×150 AA12S03-1503WT

Particle size（µm）

Pore size（Å）


S-3 120 4.6× 75 AA12S03-L546WT4.6×100 AA12S03-1046WT4.6×150 AA12S03-1546WT6.0×100 AA12S03-1006WT6.0×150 AA12S03-1506WT

A p p l i c a t i o n （S930422D） A p p l i c a t i o n （G910523Q）


第01章.indd 44 11.1.28 10:41:23 AM

45

1

Reversed-P

hase C1

8 C

olumns

（OD

S

）


Pore size（Å）


S-5 120 1.0×150 AA12S05-1501WT1.0×250 AA12S05-2501WT1.5×150 AA12S05-15P5WT1.5×250 AA12S05-25P5WT2.0× 75 AA12S05-L502WT2.0×150 AA12S05-1502WT2.0×250 AA12S05-2502WT3.0×150 AA12S05-1503WT3.0×250 AA12S05-2503WT4.6× 75 AA12S05-L546WT4.6×100 AA12S05-1046WT4.6×150 AA12S05-1546WT4.6×250 AA12S05-2546WT4.6×300 AA12S05-3046WT6.0×100 AA12S05-1006WT6.0×150 AA12S05-1506WT6.0×250 AA12S05-2506WT6.0×300 AA12S05-3006WT

200 4.6×150 AA20S05-1546WT4.6×250 AA20S05-2546WT

300 1.0×150 AA30S05-1501WT1.0×250 AA30S05-2501WT1.5×150 AA30S05-15P5WT1.5×250 AA30S05-25P5WT2.0× 75 AA30S05-L502WT2.0×150 AA30S05-1502WT2.0×250 AA30S05-2502WT4.6× 75 AA30S05-L546WT4.6×100 AA30S05-1046WT4.6×150 AA30S05-1546WT4.6×250 AA30S05-2546WT4.6×300 AA30S05-3046WT6.0×100 AA30S05-1006WT6.0×150 AA30S05-1506WT6.0×250 AA30S05-2506WT6.0×300 AA30S05-3006WT

S-10 120 4.6×100 AA12S11-1046WT4.6×150 AA12S11-1546WT4.6×250 AA12S11-2546WT4.6×300 AA12S11-3046WT6.0×100 AA12S11-1006WT6.0×150 AA12S11-1506WT6.0×250 AA12S11-2506WT6.0×300 AA12S11-3006WT

300 4.6×100 AA30S11-1046WT4.6×150 AA30S11-1546WT4.6×250 AA30S11-2546WT4.6×300 AA30S11-3046WT6.0×100 AA30S11-1006WT6.0×150 AA30S11-1506WT6.0×250 AA30S11-2506WT6.0×300 AA30S11-3006WT


Pore size（Å）


S-5 120 10×150 AA12S05-1510WT10×250 AA12S05-2510WT10×300 AA12S05-3010WT20×100 AA12S05-1020WT20×150 AA12S05-1520WT20×250 AA12S05-2520WT

200 20×150 AA20S05-1520WT20×250 AA20S05-2520WT

300 10×150 AA30S05-1510WT10×250 AA30S05-2510WT10×300 AA30S05-3010WT20×150 AA30S05-1520WT20×250 AA30S05-2520WT


Pore size（Å）


S-10 120 10×150 AA12S11-1510WT10×250 AA12S11-2510WT10×300 AA12S11-3010WT20×150 AA12S11-1520WT20×250 AA12S11-2520WT

200 20×150 AA20S11-1520WT20×250 AA20S11-2520WT

300 10×150 AA30S11-1510WT10×250 AA30S11-2510WT10×300 AA30S11-3010WT20×150 AA30S11-1520WT20×250 AA30S11-2520WT


Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)S-3 2.0×10 AA12S03-0102CC

4.0×23 AA12S03-G304CC

S-5 120 1.0×10 AA12S05-0101CC1.5×10 AA12S05-01P5CC2.0×10 AA12S05-0102CC4.0×23 AA12S05-G304CC

200 4.0×23 AA20S05-G304CC

300 1.0×10 AA30S05-0101CC1.5×10 AA30S05-01P5CC2.0×10 AA30S05-0102CC4.0×23 AA30S05-G304CC



XPCHSMW


XPCHW

YMC guard cartridge packS-3 120 4.0×23 AA12S03-G304CCPK

S-5 120 4.0×23 AA12S05-G304CCPK


Guard columnsS-3 120 4.0×10 AA12S03-0104WFG

5.0×10 AA12S03-0105WFG

S-5 120 4.0×10 AA12S05-0104WFG5.0×10 AA12S05-0105WFG 10×30 AA12S05-0310WTG 20×50 AA12S05-0520WTG

200 4.0×10 AA20S05-0104WFG 20×50 AA20S05-0520WTG

300 4.0×10 AA30S05-0104WFG5.0×10 AA30S05-0105WFG 10×30 AA30S05-0310WTG 20×50 AA30S05-0520WTG

S-10 120 4.0×10 AA12S11-0104WFG5.0×10 AA12S11-0105WFG 10×30 AA12S11-0310WTG 20×50 AA12S11-0520WTG

200 4.0×10 AA20S11-0104WFG5.0×10 AA20S11-0105WFG 20×50 AA20S11-0520WTG

300 4.0×10 AA30S11-0104WFG5.0×10 AA30S11-0105WFG 10×30 AA30S11-0310WTG 20×50 AA30S11-0520WTG

Analytical columns Semi-preparative columns


第01章.indd 45 11.1.28 10:41:24 AM

46

1

Reversed-P

hase C1

8 C

olumns

（OD

S

）

Analytical columns

YMC-Pack ODS-AM● Similar to ODS-A in selectivity● Excellent reproducibility● Useful for quality control purposes

O D S w i t h o u t s t a n d i n g l o t - t o - l o t r e p r o d u c i b i l i t yYMC-Pack ODS-AM is a product which especially emphasizes lot-to-lot reproducibility of the packing material. Strict control is performed on all aspects of quality, including physical properties of silica gel base

and surface modifying processes, in order to ensure stable quality. Double endcapping is conducted to ensure that lot-to-lot reproducibility is maintained.


Special attention is given to lot-to-lot reproducibility

α (M

ethy

lpar

ben

/2,6

-Dim

ethy

lpyr

idin

e)

10

8

6

4

2

01 2 3 4 5 6 7 8 9 10

Lot Number

The separation factor (α) of methylparaben/2,6-dimethylpyridine for 10 lots of ODS-AM packing material is plotted on the left graph. Excellent lot-to-lot reproducibility is achieved even in separation of basic compounds.

Acidic compounds

1

2

4

5

3

1. Uracil2. Benzoic acid3. p-Toluic acid4. p-Ethylbenzoic acid5. Methy benzoate

Secondary interaction of the stationary phase surface may affect peak shapes of acidic compounds.ODS-AM can provide chromatograms without tailing peaks as shown above.


ODS with a high degree ofmetal impurities

ODS-AM

1

23 1

2

3

1. Uracil2. Acetylacetone3. 8-Quinolinol

The peak shapes of coordination compounds, including 8-quinolinol and acetylacetone, may be affected by the presence of metal impurities on the surface of the silica. The distribution level of metal impurities on the column is strictly controlled for ODS-AM, providing excellent peak symmetry.

第01章.indd 46 11.1.28 10:41:26 AM

47

1

Reversed-P

hase C1

8 C

olumns

（OD

S

）

1

2

34

5

6

7

89

20 40 60 800 min

Alkaloids1. Hordenine sulfate2. Pilocarpine chloride3. Scopolamine bromide4. Atropine sulfate5. Cinchonine6. Sparteine sulfate7. Quinine8. Papaverine chloride9. Narcotine chloride

Column ：YMC-Pack ODS-AM (5 µm, 120 Å) 　150×4.6 mmI.D.Eluent ：acetonitrile/20 mM KH2PO4 (20/80)Flow rate ：1.0 mL/minTemperature ：30 ℃Detection ：UV at 210 nm

A p p l i c a t i o n （G910523J）


Pore size（Å）


S-3 120 2.0× 35 AM12S03-H502WT2.0× 50 AM12S03-0502WT2.0× 75 AM12S03-L502WT2.0×100 AM12S03-1002WT2.0×150 AM12S03-1502WT3.0× 50 AM12S03-0503WT3.0×100 AM12S03-1003WT3.0×150 AM12S03-1503WT4.6× 75 AM12S03-L546WT4.6×100 AM12S03-1046WT4.6×150 AM12S03-1546WT6.0×100 AM12S03-1006WT6.0×150 AM12S03-1506WT6.0×250 AM12S03-2506WT

S-5 120 1.0×150 AM12S05-1501WT1.0×250 AM12S05-2501WT1.5×150 AM12S05-15P5WT1.5×250 AM12S05-25P5WT2.0× 75 AM12S05-L502WT2.0×100 AM12S05-1002WT2.0×150 AM12S05-1502WT2.0×250 AM12S05-2502WT3.0×150 AM12S05-1503WT3.0×250 AM12S05-2503WT4.6× 75 AM12S05-L546WT4.6×100 AM12S05-1046WT4.6×150 AM12S05-1546WT4.6×250 AM12S05-2546WT4.6×300 AM12S05-3046WT6.0×100 AM12S05-1006WT6.0×150 AM12S05-1506WT6.0×250 AM12S05-2506WT6.0×300 AM12S05-3006WT

Semi-preparative columnsS-5 120 10×150 AM12S05-1510WT

10×250 AM12S05-2510WT10×300 AM12S05-3010WT20×100 AM12S05-1020WT20×150 AM12S05-1520WT20×250 AM12S05-2520WT


Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)Particle size（µm）

Pore size（Å）


S-3 120 2.0×10 AM12S03-0102CC4.0×23 AM12S03-G304CC

S-5 120 1.0×10 AM12S05-0101CC1.5×10 AM12S05-01P5CC2.0×10 AM12S05-0102CC4.0×23 AM12S05-G304CC



XPCHSMW


XPCHW

YMC guard cartridge packS-3 120 4.0×23 AM12S03-G304CCPK

S-5 120 4.0×23 AM12S05-G304CCPK


Guard columnsS-3 120 4.0×10 AM12S03-0104WFG

5.0×10 AM12S03-0105WFG

S-5 120 4.0×10 AM12S05-0104WFG5.0×10 AM12S05-0105WFG 10×30 AM12S05-0310WTG 20×50 AM12S05-0520WTG


第01章.indd 47 11.1.28 10:41:27 AM

48

1

Reversed-P

hase C1

8 C

olumns

（OD

S

）

Analytical columns

YMC-Pack ODS-AQ● Useful for separation of hydrophilic compounds● Can be used with 100% water in mobile phase● Different selectivity from conventional ODS

H y d r o p h i l i c O D SYMC-Pack ODS-AQ has moderate hydrophobicity and hydrogen-bonding capacity. It shows different retention behavior from that of YMC-Pack ODS-A, for samples with relatively high hydrophilicity.

It is useful in fields including carbohydrate chemistry for oligosaccharides and glycosides, pharmacognosy and natural product chemistry.

■Pore size：120 Å, 200 Å■Carbon content：14％ , 10%■Usable pH range：2.0 〜 7.5

Useful for separation of sugars

Column ：YMC-Pack ODS-AQ (5 µm, 120 Å) 　150×4.6 mmI.D.Eluent ：methanol/water (5/995)Flow rate ：1.0 mL/minTemperature ：37 ℃Detection ：RI, 8×10-6RIU/FS

This product is useful for separations using mobile phases in which water content is relatively high, such as separation of sugars and glycosides.

1. Maltoundecaose（G11）2. Maltododecaose（G12）3. Maltotridecaose（G13）4. Maltotetradecaose（G14）5. Maltopentadecaose（G15）

（M910604M）

Hydrogen-bonding capacity

ODS-A

3

2

1

4 5

Chromatograms of purine alkaloids are shown on the left.As compared with ODS-A, ODS-AQ and ODS-AL show longer retention of caffeine and theophylline relative to phenol, an internal standard.

ODS-AQ

3

2

1

45

ODS-AL

3

2

1

4

5

G11

G12

G13

G14

G15

10 20 30 min

1. Trigonelline2. Theobromine3. Theophylline4. Caffeine5. Phenol

1.8

1.6

1.4

1.2

1.0

3.0 3.1 3.2 3.3 3.4

α ( P

heno

l/Caf

fein

e) ODS-A

ODS-AQ

ODS-AL

(60 ℃) (20 ℃)10 T,K/3 -1

Change of separation factor (α) of caffeine/phenol according to temperature change in ODS-AQ, ODS-A and ODS-AL is plotted on the graph shown on the left. Although caffeine is considered to have stronger hydrogen-bonding properties than phenol, the hydrogen-bonding capacity of stationary phase may affect the α of caffeine/phenol. The smaller the hydrogen-bonding capacities are, the higher α of phenol/caffeine. ODS-AQ shows different retention behavior from that of ODS-A since it has moderate hydrogen-bonding capacity.

第01章.indd 48 11.1.28 10:41:30 AM

49

1

Reversed-P

hase C1

8 C

olumns

（OD

S

）

1

23 4

5

67

8

10 20 30 400 min

Crude drugs (glycosides)1. Arbutin2. Salicin3. Esculine4. G-Strophantine(Ouabain)5. Baicalin6. Barbaloin7. Glycyrrhizin8. Digitoxine

Column ：YMC-Pack ODS-AQ (5 µm, 120 Å) 　250×4.6 mmI.D.Eluent ：A) methanol/20 mM NH4H2PO4 (5/95) 　B) methanol/20 mM NH4H2PO4 (80/20) 　0-100％ B (0-20 min,linear),100％ B (20-40 min)Flow rate ：0.6 mL/minTemperature ：30 ℃Detection ：UV at 250 nm

A p p l i c a t i o n （G910607E）


Pore size（Å）


S-3 120 2.0× 35 AQ12S03-H502WT2.0× 50 AQ12S03-0502WT2.0× 75 AQ12S03-L502WT2.0×100 AQ12S03-1002WT2.0×150 AQ12S03-1502WT3.0× 50 AQ12S03-0503WT3.0×100 AQ12S03-1003WT3.0×150 AQ12S03-1503WT4.6×100 AQ12S03-1046WT4.6×150 AQ12S03-1546WT6.0×100 AQ12S03-1006WT6.0×150 AQ12S03-1506WT

S-5 120 1.0×150 AQ12S05-1501WT1.0×250 AQ12S05-2501WT1.5×150 AQ12S05-15P5WT1.5×250 AQ12S05-25P5WT2.0× 75 AQ12S05-L502WT2.0×150 AQ12S05-1502WT2.0×250 AQ12S05-2502WT3.0×150 AQ12S05-1503WT3.0×250 AQ12S05-2503WT4.6× 75 AQ12S05-L546WT4.6×100 AQ12S05-1046WT4.6×150 AQ12S05-1546WT4.6×250 AQ12S05-2546WT4.6×300 AQ12S05-3046WT6.0×100 AQ12S05-1006WT6.0×150 AQ12S05-1506WT6.0×250 AQ12S05-2506WT6.0×300 AQ12S05-3006WT

200 4.6×150 AQ20S05-1546WT4.6×250 AQ20S05-2546WT

Semi-preparative columnsS-5 120 10×150 AQ12S05-1510WT

10×250 AQ12S05-2510WT10×300 AQ12S05-3010WT20×100 AQ12S05-1020WT20×150 AQ12S05-1520WT20×250 AQ12S05-2520WT

200 20×150 AQ20S05-1520WT20×250 AQ20S05-2520WT

S-10 120 10×150 AQ12S11-1510WT10×250 AQ12S11-2510WT10×300 AQ12S11-3010WT20×150 AQ12S11-1520WT20×250 AQ12S11-2520WT

200 20×150 AQ20S11-1520WT20×250 AQ20S11-2520WT



Pore size（Å）


S-3 120 2.0×10 AQ12S03-0102CC4.0×23 AQ12S03-G304CC

S-5 120 1.0×10 AQ12S05-0101CC1.5×10 AQ12S05-01P5CC2.0×10 AQ12S05-0102CC4.0×23 AQ12S05-G304CC

200 4.0×23 AQ20S05-G304CCA guard cartridge holder will need to be purchased separately before using this product for the first time.


XPCHSMW


XPCHW

YMC guard cartridge packS-3 120 4.0×23 AQ12S03-G304CCPK

S-5 120 4.0×23 AQ12S05-G304CCPK


Guard columnsS-3 120 4.0×10 AQ12S03-0104WFG

5.0×10 AQ12S03-0105WFG

S-5 120 4.0×10 AQ12S05-0104WFG5.0×10 AQ12S05-0105WFG 10×30 AQ12S05-0310WTG 20×50 AQ12S05-0520WTG

200 4.0×10 AQ20S05-0104WFG 20×50 AQ20S05-0520WTG

S-10 120 10×30 AQ12S11-0310WTG 20×50 AQ12S11-0520WTG

200 20×50 AQ20S11-0520WTG


第01章.indd 49 11.1.28 10:41:32 AM

50

1

Reversed-P

hase C1

8 C

olumns

（OD

S

）

Analytical columns

YMC-Pack ODS-AL● ODS with residual silanol groups● Utilizes secondary interaction caused by silanol groups

N o n - e n d c a p p e d O D SYMC-Pack ODS-AL uses not only hydrophobic interaction but also secondary interaction caused by silanol groups that affect separation. This results in a different selectivity from conventional ODS columns.

When ionic interaction is utilized, it is preferable to use a buffer in the mobile phase to achieve reproducibility of chromatograms.


Effects of silanol group

ODS-AL

1

2

3

4

α(4/3)=1.46

The separation factor (α) of internal standards methylparaben/2,6-dimethylpyridine for ODS-AM is different from that of ODS-AL, which is not endcapped. ODS-AL, which is affected by residual silanol, shows relatively great retention of pyridines.

ODS-AM

1 2 3

4

α(4/3)=2.801. Uracil2. Pyridine3. 2,6-Dimethylpyridine4. Methylparaben

Utilizes residual silanol groups for separation

ODS-AL

3

4

2

1

0 5 10 15 20 min

The figure shows separation of disinfectants under the conditions described in the Japanese Pharmacopeia. The object is to select the column permitting the elution of benzoic acid, salicylic acid and theophylline, in this order, insuring complete separation of these peaks. With ODS-AQ, separation of salicylic acid and theophylline is incomplete. By contrast, ODS-AL provides excellent separation. Thus, ODS-AL may provide excellent separation when the separation conditions cannot be optimized on other ODS columns.

ODS-AQ

4

2,3

1

0 5 10 15 20 min

Column ：YMC-Pack ODS-AL and ODS-AQ (5 µm, 120 Å) 　150×4.6 mmI.D.Eluent ：100 mM KH2PO4-Na2HPO4 (pH 7.0)/methanol (75/25)Flow rate ：0.8 mL/minTemperature ：30 ℃Detection ：UV at 270 nm(Conditions described in Japanese Pharmacopoeia 15th ed.)

PhenolTheophylline (I.S.)

4.3.

OH

Salicylic acid

2.1. COOH

OHBenzoic acid

COOH

HO

O

CH

CH

N N

NN3

3

Disinfectants

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Reversed-P

hase C1

8 C

olumns

（OD

S

）

0 10 20 30 40 min

1

3

2Phenacyl esters of bile acids from pig gall1. Cholic acid (CA)2. Hyodeoxycholic acid (HDCA)3. Chenodeoxycholic acid (CDAC)

Column ： YMC-Pack ODS-AL (5 µm, 120 Å) 250×4.6 mmI.D.

Eluent ：acetonitrile/water (60/40)Flow rate ：1.0 mL/minTemperature ：40 ℃Detection ：UV at 240 nm

A p p l i c a t i o n （G900417B）


Pore size（Å）


S-5 120 1.0×150 AL12S05-1501WT1.0×250 AL12S05-2501WT1.5×150 AL12S05-15P5WT1.5×250 AL12S05-25P5WT2.0×150 AL12S05-1502WT2.0×250 AL12S05-2502WT4.6× 75 AL12S05-L546WT4.6×100 AL12S05-1046WT4.6×150 AL12S05-1546WT4.6×250 AL12S05-2546WT4.6×300 AL12S05-3046WT6.0×100 AL12S05-1006WT6.0×150 AL12S05-1506WT6.0×250 AL12S05-2506WT6.0×300 AL12S05-3006WT

Semi-preparative columnsS-5 120 10×150 AL12S05-1510WT

10×250 AL12S05-2510WT10×300 AL12S05-3010WT20×100 AL12S05-1020WT20×150 AL12S05-1520WT20×250 AL12S05-2520WT



Pore size（Å）


S-5 120 1.0×10 AL12S05-0101CC1.5×10 AL12S05-01P5CC2.0×10 AL12S05-0102CC4.0×23 AL12S05-G304CC



XPCHSMW


XPCHW

Guard columnsS-5 120 4.0×10 AL12S05-0104WFG

5.0×10 AL12S05-0105WFG10×30 AL12S05-0310WTG20×50 AL12S05-0520WTG


第01章.indd 51 11.1.28 10:41:36 AM

52

1

Reversed-P

hase C1

8 C

olumns

（OD

S

）

Analytical columns

J’sphere ODS-H80, ODS-M80, ODS-L80● Useful for method development● High theoretical plate number

T h r e e t y p e s o f O D S , e a c h w i t h d i f f e r e n t l i g a n d c o v e r a g eJ’sphere offers a choice from three kinds of ODS made from the same silica gel base, each with different ligand coverage.Differences in ligand coverage considerably affect the hydrophobic retention behavior of solutes, as well as the separation behavior

resulting from solute functional groups or tertiary structure.J’sphere is useful for the optimization of separation conditions, since there is almost no need to consider interactions other than hydrophobic and hydrogen-bonding interactions (e.g., ionic or coordinate interaction).

■Pore size：80 Å■Carbon content：ODS-H80 22％ , ODS-M80 14% , ODS-L80 9％■Usable pH range：ODS-H80 1.0 〜 9.0, ODS-M80・L80 2.0 〜 7.5

Three types of ODS

Column ：J’sphere 　150×4.6 mmI.D.Eluent ：acetonitrile/water (75/25)Flow rate ：1.0 mL/minTemperature ：37 ℃Detection ：UV at 254 nm

ODS-H80

ODS-M80

ODS-L80

0 10 20 30 40 50 min

n 2n+1C (n=0-8)

Alkylbenzenes

H

Differences in separation characteristics

Column ：Eluent ：Flow rate ：Temperature ：Detection ：

150×4.6 mmI.D.acetonitrile/water (75/25)1.0 mL/min37 ℃UV at 254 nm

Column ：Eluent ：Flow rate ：Temperature ：Detection ：

150×4.6 mmI.D.acetonitrile/20 mM KH2PO4 (15/85)1.0 mL/min37 ℃UV at 254 nm

10 20

16.5 29.6

15.9

15.2

15.2

15.1

15.0

13.1

6.2

300k’(octylbenzene)

J’sphere ODS-H80

J’sphere ODS-M80

J’sphere ODS-M80

J’sphere ODS-L80

J’sphere ODS-H80BRAND-B1BRAND-F1YMC-Pack ODS-AMBRAND-G1J’sphere ODS-M80BRAND-A2BRAND-C3

J’sphere ODS-L80

0.20 0.25

0.24

0.21

0.24

0.25

0.25

0.25

0.27

0.30

0.30

0.30α(caffeine/phenol)

J’sphere ODS-H80BRAND-F1BRAND-G1YMC-Pack ODS-AMBRAND-C3BRAND-B1J’sphere ODS-M80BRAND-A2J’sphere ODS-L80

Comparison

of

hydrophobicity

of

ODS

columns

produced

by

various

manufacturers

Comparison

of

hydrogen

bonding

capacity

of

ODS

columns

produced

by

various

manufacturers

ODS columns with different ligand coverage have different hydrophobicity, hydrogen bonding capacity and steric selectivity for tertiary structures. ODS-H80 has exceptionally high hydrophobicity and low hydrogen bonding capacity, ODS-M80 has moderate hydrophobicity and hydrogen bonding capacity and ODS-L80 has low hydrophobicity, comparable to that of C8 and high hydrogen bonding capacity. J’sphere can be used with confidence to identify structural differences of solutes and is useful for improving the efficiency of separation optimization.

ODS-H80 ODS-M80 ODS-L80

Image of stationary phase

第01章.indd 52 11.1.28 10:41:39 AM

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1

Reversed-P

hase C1

8 C

olumns

（OD

S

）

Analytical columnsPacking material


Pore size（Å）


ODS-H80 S-4 80 1.0×150 JH08S04-1501WT1.0×250 JH08S04-2501WT1.5×150 JH08S04-15P5WT1.5×250 JH08S04-25P5WT2.0× 75 JH08S04-L502WT2.0×100 JH08S04-1002WT2.0×150 JH08S04-1502WT2.0×250 JH08S04-2502WT3.0×150 JH08S04-1503WT3.0×250 JH08S04-2503WT4.6× 75 JH08S04-L546WT4.6×150 JH08S04-1546WT4.6×250 JH08S04-2546WT6.0×150 JH08S04-1506WT6.0×250 JH08S04-2506WT

ODS-M80 S-4 80 1.0×150 JM08S04-1501WT1.0×250 JM08S04-2501WT1.5×150 JM08S04-15P5WT1.5×250 JM08S04-25P5WT2.0× 75 JM08S04-L502WT2.0×100 JM08S04-1002WT2.0×150 JM08S04-1502WT2.0×250 JM08S04-2502WT3.0×150 JM08S04-1503WT3.0×250 JM08S04-2503WT4.6× 75 JM08S04-L546WT4.6×150 JM08S04-1546WT4.6×250 JM08S04-2546WT6.0×150 JM08S04-1506WT6.0×250 JM08S04-2506WT

ODS-L80 S-4 80 1.0×150 JL08S04-1501WT1.0×250 JL08S04-2501WT1.5×150 JL08S04-15P5WT1.5×250 JL08S04-25P5WT2.0× 35 JL08S04-H502WT2.0× 75 JL08S04-L502WT2.0×100 JL08S04-1002WT2.0×150 JL08S04-1502WT2.0×250 JL08S04-2502WT3.0×150 JL08S04-1503WT3.0×250 JL08S04-2503WT4.6× 75 JL08S04-L546WT4.6×150 JL08S04-1546WT4.6×250 JL08S04-2546WT6.0×150 JL08S04-1506WT6.0×250 JL08S04-2506WT

Semi-preparative columnsPacking material


Pore size（Å）


ODS-H80 S-4 80 10×150 JH08S04-1510WT10×250 JH08S04-2510WT20×150 JH08S04-1520WT20×250 JH08S04-2520WT

ODS-M80 S-4 80 10×150 JM08S04-1510WT10×250 JM08S04-2510WT20×150 JM08S04-1520WT20×250 JM08S04-2520WT

ODS-L80 S-4 80 10×150 JL08S04-1510WT10×250 JL08S04-2510WT20×150 JL08S04-1520WT20×250 JL08S04-2520WT

Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)ODS-H80 S-4 80 1.0×10 JH08S04-0101CC

1.5×10 JH08S04-01P5CC2.0×10 JH08S04-0102CC4.0×23 JH08S04-G304CC

ODS-M80 S-4 80 1.0×10 JM08S04-0101CC1.5×10 JM08S04-01P5CC2.0×10 JM08S04-0102CC4.0×23 JM08S04-G304CC

ODS-L80 S-4 80 1.0×10 JL08S04-0101CC1.5×10 JL08S04-01P5CC2.0×10 JL08S04-0102CC4.0×23 JL08S04-G304CC



XPCHSMW


XPCHW

YMC guard cartridge packODS-H80 S-4 80 4.0×23 JH08S04-G304CCPK

ODS-M80 S-4 80 4.0×23 JM08S04-G304CCPK

ODS-L80 S-4 80 4.0×23 JL08S04-G304CCPK


Guard columnsODS-H80 S-4 80 10×30 JH08S04-0310WTG

20×50 JH08S04-0520WTG

ODS-M80 S-4 80 10×30 JM08S04-0310WTG20×50 JM08S04-0520WTG

ODS-L80 S-4 80 10×30 JL08S04-0310WTG20×50 JL08S04-0520WTG


第01章.indd 53 11.1.28 10:41:40 AM

54

1

Reversed-P

hase C1

8 C

olumns

（OD

S

）

YMC-Pack PolymerC18● Utilizes polymer base which is not affected by silanol● Different separation characteristics from silica-based ODS● Excellent pH stability

P o l y m e r t y p e C18PolymerC18 is a C18 column made from methacrylate polymer. It has excellent pH stability and it is useful for separation of basic compounds because there are no silanol or metal impurities to cause secondary interaction.

Since π electrons of the carbonyl group or the hydroxyl group on the surface of the base material show particular interactions with solutes, PolymerC18 can show different separation characteristics from silica-based ODS.

■Usable pH range：2.0 〜 13.0

Analytical columns

Excellent resistance to acid and alkali

Alkali resistance

1

23

1

23

〈Prior to eluent flow〉〈After 6 hours〉

0 10 20 min 0 10 20 min

Column ：Eluent ：Flow rate ：Temperature ：Time ：

YMC-Pack PolymerC18 150×4.6 mmI.D.0.1N NaOH (pH 13)/acetonitrile (80/20)0.5 mL/minambient6 hours

〈Flow conditions〉

Eluent ：Flow rate ：Temperature ：Detection ：Sample ：

acetonitrile/water (60/40)0.5 mL/min30 ℃UV at 270 nm1.Uracil2.Methyl benzoate3.Butyl benzoate

〈Analytical conditions〉

Acid resistance1

23

1

23

〈Prior to eluent flow〉〈After 100 hours〉

0 10 20 min 0 10 20 min

Column ：Eluent ：Flow rate ：Temperature ：Time ：

YMC-Pack PolymerC18 150×4.6 mmI.D.100 mM H3PO4 (pH 1.7)0.5 mL/minambient100 hours

〈Flow conditions〉

Eluent ：Flow rate ：Temperature ：Detection ：Sample ：

acetonitrile/water (60/40)0.5 mL/min30 ℃UV at 270 nm1.Uracil2.Methyl benzoate3.Butyl benzoate

〈Analytical conditions〉

Can be used with alkali mobile phase1. Barbital2. Phenobarbital3. Pentobarbital4. Hexobarbital5. Secobarbital

Column ：YMC-Pack PolymerC18 　150×4.6 mmI.D.Eluent ：50 mM Na2HPO4-Na3PO4 (pH 11.0)/methanol (75/25)Flow rate ：0.5 mL/minTemperature ：30 ℃Detection ：UV at 254 nm

1

2

3

4

5

0 10 20 min

第01章.indd 54 11.1.28 10:41:43 AM

55

1

Reversed-P

hase C1

8 C

olumns

（OD

S

）

Different separation characteristics from silica-based ODS (aromatic compounds)

Column ：YMC-Pack PolymerC18 and YMC-Pack ODS-A 　150×6.0 mmI.D.Eluent ：methanol/water (40/60)Flow rate ：1.0 mL/minTemperature ：30 ℃Detection ：UV at 280 nm

PolyphenolYMC-Pack PolymerC18

1

2

3

0 10 20 30 min

YMC-Pack ODS-A

1

2

3

0 5 10 min

1. 2. 3.

Phloroglucinol Resorcinol Phenol

HO OH

OH

OH

OH OH

Different separation characteristics from silica-based ODS (unsaturated compounds)

Column ：YMC-Pack PolymerC18 and YMC-Pack ODS-AM 　150×4.6 mmI.D.Eluent ：methanol/water (45/55) for PolymerC18 　methanol/water (50/50) for ODS-AMFlow rate ：0.8 mL/minTemperature ：50 ℃Detection ：UV at 210 nm

Triterpene saponinsYMC-Pack PolymerC18

1

2

0.00 7.50 15.00 22.50 min

YMC-Pack ODS-AM

22.50 min15.007.500.00

1,2COO-β-D-Glc

CH OH2

HO

HO

1.

18

COO-β-D-Glc

CH OH2

HO

HO

2. 19

Analytical columnsParticle size

（µm）Column size

inner diameter×length（mm） Product number

S-6 2.0× 75 PC99S06-L502WT2.0×150 PC99S06-1502WT4.6×150 PC99S06-1546WT4.6×250 PC99S06-2546WT6.0×150 PC99S06-1506WT6.0×250 PC99S06-2506WT

S-10 4.6×250 PC99S10-2546WT4.6×300 PC99S10-3046WT6.0×250 PC99S10-2506WT6.0×300 PC99S10-3006WT

Semi-preparative columnsS-6 10×250 PC99S06-2510WT

S-10 10×250 PC99S10-2510WT20×300 PC99S10-3020WT

Guard cartridge columns (with inner diameter 2.0 mm: 2/pack, 4.0 mm: 3/pack)Particle size

（µm）Column size

inner diameter×length（mm） Product number

S-6 2.0×10 PC99S06-0102CC4.0×23 PC99S06-G304CC



XPCHSMW


XPCHW

Guard columnsS-6 4.0×10 PC99S06-0104WFG

5.0×10 PC99S06-0105WFG 10×30 PC99S06-0310WTG

S-10 4.0×10 PC99S10-0104WFG5.0×10 PC99S10-0105WFG 10×30 PC99S10-0310WTG 20×50 PC99S10-0520WTG


第01章.indd 55 11.1.28 10:41:47 AM

第01章.indd 56 11.1.28 10:41:47 AM

reversed-phase c18 columns (ods)

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