research infrastructures in structural biology - nmr lucia banci cerm, florence, italy...

Research Infrastructures in

Structural Biology - NMR

Lucia BanciCERM, Florence, Italy

[email protected]

Workshop "Future Needs for Research Infrastructures in Biomedical Sciences“, Brussels, 16-03-05

Biomolecular structures determination• First X-ray protein structures (myoglobin and hemoglobin) in the late 1950’s (Perutz and Kendrew, Nobel Prize 1962)

Protein Data Bank (PDB) created in 1970-1971

• First NMR protein structure (Proteinase Inhibitor IIa) in 1985 (Wüthrich, Nobel Prize 2002)

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1974 1988 2000 2004

NMRX-ray


but……

NMR goes beyond structure !

.dynamics, protein-protein interactions,

EU NMR Infrastructures in Structural Biology

Equipped with: spectrometers from 900 MHz down, 900, 800, and 500MHz with Cryoprobes a WB 700 MHz for SSNMR

CERM, Florence, IT

Equipped with: spectrometers from 900 MHz down (a 600MHz with cryoprobe)

SONNMR, Utrecht, NL

Equipped with: spectrometers from 900 MHz down, several with Cryoprobes a WB 600 MHz for SSNMR

BMRZ, Frankfurt, DManchester, UK

Paris, F

Ljubljana, SLO

Göteborg, S

Helsinki, FIN

Madrid, E

Lyon, F


Previous Funding to the three EU NMR RI (1994-2004)

Access is still provided on voluntary basis to meet the request of the scientific community

FP3 Limited Transnational access funded to the individual Infrastructures (RI)

FP6 Funding for access has been discontinued • “Design study” project under negotiation

FP4 Transnational access funded to each RI which formed a cluster • Concerted Action involving other EU NMR laboratories

• RTD’s

FP5 Transnational access funded to each RI which formed a cluster • Cluster formalization through ICN common access protocol • RTD’s


Future Needs for existing NMR RI

• Support for technological/methodological advancement of the Infrastructures

“Design studies” are an excellent instrument for forefront advancement of RI

• More funds for instruments: – keep the instrumentation updated

EC could support about 20% depreciation cost/year

– acquire new advanced equipment

• Reestablish access for European researchers:

– Remote Access should be promoted

Increased support for technical staff


Future Scientific Needs

Integrated Structural Biology Infrastructure for Europe


Bioinformatics Clinical records

PrioritizationModule

Structural Biology

HT Expression Module

Chemical Biology

Biomedical Applications

FESP Forum for European Structural Proteomics

www.ec-fesp.org

Lucia Banci, Centro Risonanze Magnetiche, University of Florence, Italy

Wolfgang Baumeister, Max-Planck-Institute of Biochemistry, Martinsried, Germany

Udo Heinemann, Max-Delbrück-Centrum für Molekulare Medizin, Berlin Germany

Gunter Schneider, Karolinska Institutet, Stockholm Sweden

Joel Sussman, Weizmann Institute of Science, Rehovot Israel - Coordinator


Goals

• Assess Structural Genomics/Proteomics projects already in action at national, European, and global level.

• Assess existing Infrastructures (synchrotrons, NMR, EM,

Protein Production, Bioinformatics) with respect to the needs for Structural Genomics/Proteomics in Europe, and comparison with the status in the rest of the world

• Develop strategic plans for an European policy in Structural Genomics/ Proteomics


Outcomes

• A database of the Structural Genomics/Proteomics projects worldwide

• A position paper which includes -- assessment of Structural Genomics/Proteomics Infrastructures and activities in Europe and worldwide

-- a strategic road map to guide future directions of SG and SP initiatives in EU


To achieve these goals the active contribution of the entire SG/SP community is needed and sought for

Contributions will be collected from:

visits to the Infrastructures involved in SG/SP activities installation of a web site www.ec-fesp.org collecting contributions to the debate targeted workshops


research infrastructures in structural biology - nmr lucia banci cerm, florence, italy...

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