replicazionedel dna - people.unica.it · dna replication in e. coli and other prokaryotes and in...
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Replicazione del DNA
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Esperimento di Meselson e Stahl
(1958)
14N = azoto leggero
15N = azoto pesante
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Processività: caratteristica degli enzimi che operano susubstrati polimerici.
Nel caso della DNA-polimerasi è definita dal numero (medio) di desossi-nucleotidi che l’enzima aggiunge ogni volta che silega alla struttura primer-stampo.
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Ogni DNA-polimerasi ha una propria caratteristicaprocessività, che varia da pochi nucleotidi a oltre 50.000 nucleotidi aggiunti per evento di legame.
La tappa limitante è data dal legame iniziale (circa 1 secondo).
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Fase di Inizio
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Fase di Elongazione
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