rekayasa genetik bakteri
TRANSCRIPT
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REKAYASA GENETIKA BAKTERI
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TRANSFER DNA PD BAKTERI
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RECOMBINAN GENETIK
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ENDONUKLEASE RESTRIKSI
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DNA LIGASE:• Enzim yg memperbaiki potongan DNA
& menggabungkan 2 fragmen DNA mjd molekul
double-stranded
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TAHAP-TAHAP KLONING
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ISOLASI DNA PADA REKAYASA GENETIK
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CONTOH BBRP VEKTOR YG DIGUNAKAN PD REKAYASA GENETIK
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KLONING PD PLASMID DLM JUMLAH BESAR
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KLONING DALAM PLASMID
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PENGGUNAAN PLASMID pBR 322 SBG VEKTOR KLONING
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FAGA LAMBDA PD JALUR LITIK & LISOGENIK
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LIFE CYCLE OF A LYSOGENIC OR TEMPERATE VIRUS
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PROSEDUR PENGGUNAANBAKTERIOFAGA LAMBDASBG VEKTOR KLONING
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KLONING GEN SOMATOSTATIN DLM E. COLI Lac operon Somatostatin gene Plasmid DNA-----P---O---B-gal- TACwwwwwwwwwwwwACT------
Transcription ↓ ↓ Induction with Lactose Somatostatin mRNA ------AUGwwwwwwwwwwwwUGA-----
↓ ↓Translation B-gal-met-----Somatostatin-----stop
↓ Cyanogen bromide
B-gal-met + -----Somatostatin-----
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KLONING GEN INSULIN DLM BACILLUS SUBTILIS
Trp operon Insulin A genePlasmid DNA----P---O---trpE --TACwwwwwwwwwwwwACT------
↓ ↓ Derepression of Transcription Trp operon Insulin A mRNA mRNA------------AUGwwwwwwwwwwwwUGA------
↓ ↓Translation TrpE-met-------Insulin A-------stop
↓ Cyanogen bromide cleavage
TrpE fragments + -------Insulin A-------
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KLONING GEN INTERFERON DLM E.COLI
control elements (RPO) Interferon genePlasmid DNA---Beta-lactamase gene--TACwwwwwwwwwwwwACT------
Transcription ↓ ↓
interferon mRNA mRNA------------AUGwwwwwwwwwwwwUGA------
↓Translation met -------interferon-------stop
Cyanogen bromide cleavage ↓ met + -------interferon-------
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