reflections on genomic editing in nonhuman primates (marina emborg)

8/20/2019 Reflections on Genomic Editing in Nonhuman Primates (Marina Emborg)

1/31

_iblijtfhks hk `ikhnfj icftfk` fk khkaundk prfndtis

Ndrfkd I. Inohr`, N.C. ]a.C.

FLD_ 4?-=0-?=49


2/31

Sai dkfndl nhcil dkc ahw ft fs usic fnpdjt jlfkfjdl trdksldtfhk


3/31

@ikhnfj Icftfk` (@I) fk khk aundk prfndtis (dkc htair spijfis)

NHCILZ SAI_D]FIZ

@I shndtfj@I `irnlfki

Dpplfjdtfhks hb @I



4/31

Oibhri dpplyfk` @ikhnfj Icftfk` fk khkaundk prfndtis….

Pay ch wi ch risidrja fk

khkaundk prfndtis8

• Sh ukcirstdkc cfsidsi

•

Sh tist bfrst-fk-jldss dkc fkvdsfvi tairdpfis


5/31

Nhkeiys vs. rhcikts - Oiadvfhrdl seflls

YJ Cdvfs - K]_J Nhkeiy nhcils prhvfci hutjhni nidsuris tadt advi jlfkfjdl fnpdjt


6/31

Nhkeiys vs. rhcikts ‚ Ordfk jhnplixfty

Nhusi

_aisus Nhkeiy

Nhkeiy nhcils dllhw civilhpnikt hb fkvdsfvinitahcs dkc jdk prhvfci phst nhrtin ivfcikji hbsujjiss hr bdfluri


7/31

]drefkshk‖s cfsidsi (]C)

• ]C fs typfjdlly cisjrfoic ds d prh`rissfvi kiurhci`ikirdtfvi nhvinikt cfshrcir

dsshjfdtic th tai lhss hb CD kiurhks fk tai ZKpj dkc tai prisikji hb LOs dkc LKs

• Ndfkstdy hb tairdpy7 CD ripldjinikt.

_K

JJ

D`fk` ]C

ZK

Rirnflyid dkc Inohr`,, ?=49@hicirt it dl, ?==4


8/31

Zuostdktfd Kf`rd

Jhrtix

Chrsdl Nhthr Kujlius hb tai Rd`us

Icfk`ir-Pistpadl Kujlius

]drdkf`rdl Kujlius

Ayphtadldnus

Hlbdjthry Oulo

Lhjus Jhirulius

Kujlius Odsdlfs hb Niykirt

Zirhthkir`fj _dpai Kujlif

]icukjulhphktfki Kujlius

_itfjuldr Bhrndtfhk hbtai ]hks dkc Nfcordfk

]irfpairdl Zynpdtaitfj @dk`lfd

Fktirnicfhldtirdl Jill Jhlunkhb tai Zpfkdl Jhrc

Bftzpdtrfje it dl, Dktfhx. _ic. ?==6

]C dbbijts nultfpli JKZ dkc ]KZ drids…


9/31

]C synpthns riblijt tai jhnplixfty hb tai cfsidsi

Zwdkshk dkc Inohr`, ?==6


10/31

Padt jdusis ]C8

Pi ch kht ekhw wadt trf``irs]C

Dlpad sykprhtifkd``ri`dtfhk

taind`fjsahp.jhn.du

Afrsja it dl, ?=4:

Padt jdusis ]C8

_fse bdjthrs fciktfbfic7

•

D`fk`

•

Ikvfrhknikt

•

@ikitfjs


11/31

]usjandk, ?=4:

Duthshndl chnfkdkt

ZKJD (]D_E4, ]D_E3)

L__E? (]D_E1)

R]Z:9

IFB3@4

Duthshndl rijissfvi

]D_EFK (]D_E?)

]FKE4 (]D_E2)

CG4 (]D_E0)

@ikis rhoustly dsshjfdtic wfta ]C/]drefkshkfsn

•

Sai L__E? prhtifk fs d ldrì (?9?0 dnfkhdjfcs) @S]-riùldtic sirfki/tarihkfki efkdsitadt jhktdfks sivirdl prhtifk-prhtifk fktirdjtfhk chndfks. Ft fs prisikt fk tai ordfk fk drids

eiy th ]C. Fts rhli fs kht jlidr.

• Zfkji fts cfsjhviry fk ?==3, L__E? nutdtfhks advi oiik fciktfbfic ds tai nhst jhnnhkìkitfj jdusi hb bdnflfdl dkc khk bdnflfdl ]C (4-:=% hb dll ]C jdsis cipikcfk` hkphpuldtfhk stucfic).

• L__E?@?=46Z fs tai nhst briquikt nutdtfhk. ]dtfikts jdrryfk` tafs nutdtfhk sahw d jlfkfjdldkc kiurhpdtahlh`fjdl prhbfli fkcfstfkùfsadoli brhn sphrdcfj ]C.


12/31

]C nhkeiy nhcils jurriktly dvdfldoli

NHCIL]C

itfhlh`y Kiurhprhtijtfvi _isthrdtfvi LFCKhk nhthr

sf`ks

D`fk` 4--? ? 3 3 :

Dlpad sykujlifk `ikitrdksbir 4--? 4--? 4--? 3 9

2-HACD fktrdstrfdtdl 4--? 4--? 4--? 3 9

N]S] systinfj djuti : 3 4 4 ?

N]S] systinfjfktirnicfdti ? 3 4 4 ?

N]S] systinfj jarhkfj 4--? 3 4 4 ?

N]S] fktrdjdrhtfc ? 4--? 4 3 3

N]S] fktrdjdrhtfc + fv : : 4 ? :

Nhcil rdtfk`7 4- nhst suftdoli, 9-fkdciqudti

Jdrcfdj cysduthkhnfd nhcil fk ]C oy systinfj 2-HACD

Inohr`, FLD_ ?==0


13/31

Lfnftdtfhks hb jurrikt nhcils

Dkfndl nhcils ]C

Kiurhjainfjdl bhjus CD CD++, out dlsh KD, 9AS, Dja

Kiurhdkdthnfjdl bhjus ZKj ZKj, ZKr dkc htair JKZ dkc ]KZ systins

Jilluldr pdtahlh`y jill lhss jill lhss, liwy ohcfis

]dtah`ikisfs thxfj ukekhwk thxfj x `ikitfj fktirdjtfhk

Sfni jhursi djuti slhw prh`rissfvi

_ijhviry sf`kfbfjdkt (ukliss lisfhk jhnpliti) prh`rissfvi hkji jhnpiksdtfhk ixadustic

Htair

OOO hpikfk` dsshjfdtic th tridtnikt,`lfdl sjdrs fk fkgijtfhk sftis ****


14/31

Liktfvfrdl-odsic7

•

@B] raisus (Jadk it dl., ?==45 Phlb`dkìt dl., ?==45 Kfu it dl, ?=4=) ndrnhsit(Zdsdef it dl., ?==6).

•

Auktfk`thk‖s cfsidsi @B]+JD@ raisus

auktfk`tfk ripidt (Vdk` it dl, ?==1)

•

]C aundk D9:S-syk jykhnhl`hus (Kfu it

dl., ?=49).

Jhnpdric th htair spijfis KA] trdksìkisfs ads ld`ìc

J_FZ]_-odsic7•

]pdr-` dkc _d`4 (Kfu it dl, ?=43)

• Cujaikki sykcrhni (Jaik it dl, ?=49)• Q-lfkeic dcrikdl ayphpldsfd jhkìkftd

(DAJ) dkc ayph`hkdchtrhpfj

ayph`hkdcfsn (AA), Cdx4 ekhjehut

jykhnhl`hus bitus (Edk` it dl, ?=42)

…prh`riss,

Ndppfk` aundk dkc khk aundk ìkhni,

Fciktfbyfk` cfsidsi-dsshjfdtic ̀ ikis,

Lfnftdtfhks hb jurrikt nhcils dkc tairdpfis, dri builfk` jadkì…


15/31

]usjandk, ?=4:

Duthshndl chnfkdkt

ZKJD (]D_E4, ]D_E3)

L__E? (]D_E1)

R]Z:9

IFB3@4

Duthshndl rijissfvi

]D_EFK (]D_E?)

]FKE4 (]D_E2)

CG4 (]D_E0)

@ikis rhoustly dsshjfdtic wfta ]C/]drefkshkfsn

• Pfta ixjiptfhk hb ZKJD trfshny, ]C ads oiik lfkeic th sfk`li phfkt nutdtfhks.

•

Safs su``ists tadt tai jldssfj dpprhdja hb trdks`ikfj hvirixprissfhk hb d nutdticprhtifk ndy oi liss ibbijtfvi dt prhcujfk` tai aundk ]C paikhtypi tadk fktrhcujfk` tai

fciktfjdl dllilfj vdrfdkt bhukc fk tai aundk cfsidsi-dsshjfdtic dllili.

• @I jdk prhvfci d shlutfhk.


16/31


NHCILZ SAI_D]FIZ


Dpplfjdtfhks hb @I



17/31

]usjandk, ?=4:

Duthshndl chnfkdkt

ZKJD (]D_E4, ]D_E3)

L__E? (]D_E1)

R]Z:9

IFB3@4

Duthshndl rijissfvi

]D_EFK (]D_E?)

]FKE4 (]D_E2)

CG4 (]D_E0)

Padt `iki fs rilivdkt th tai cfsidsi8


18/31

Ndrnhsit dt cdy 49Zjaultz-Cdreik it dl, ?=49

_aisus dt cdy 49

Padt spijfis8


19/31

Inoryh

Srdksbir

rijfpfikt

FRB/FJZF

L__E? @?=46Z

Srdks`ikfj Ndrnhsits bhr Srdksldtfhkdl Ztin Jill _isidrja

f]ZJ

Sairdpiutfj

jills

D J_FZ]_ dpprhdja

Wy`htfj jythpldsnfj fkgijtfhk

@ikhnfj icftichbbsprfk`


20/31

Zjaultz-Cdreik it dl., Civ ]syja. ?=49

Jhnnhk ndrnhsit nhkeiys civilhpniktdl nflisthkis


21/31

Ch khknhthr sf`ks pricfjt ]C8

Khripfkiparfki lhss & fkjridsi fk thxfj nitdohlftis

ricujic RNDS? ixprissfhk

Lhjus jhirulius

Kihjhrtix

JKZ7 fnpdfric jh`kftfvi bukjtfhks, ciprissfhk,sliip cfsturodkji, dkxfity

Jdrcfdj/duthkhnfj7 hrtahstdtfj ayphtiksfhk, bdtf`ui, cysraytanfds

Fkftfdti padrndjhlh`fjdl hr lfbistyli strdti`fis th dttikudti burtair chpdnfkilhss & ricuji thxfj nitdohlftis

"#$#%$&'( ') %'*(&$&+# ,&*(, - '$.#/ =&,'/=#/, 4$ 4*# /4(*#

') */#4$#,$ ?" /&,:3 924*&(* ') &204&/#= #1#%6$&+#

)6(%$&'(3 "#$#%$&'( ') $'1&% 2#$47'5&$# &( 0#/&0.#/


22/31


NHCILZ SAI_D]FIZ


Dpplfjdtfhks hb @I



23/31

@B] JC21 JC: JC1

@BD]

(kh kfssli)

K f ` r d

] u t d n i k

ALD-C_

(kh kfssli)(kh Kfssl jhuktirstdfkfk`)


24/31


25/31

Jill ripldjinikt fs nhri tadk pldyfk` d puzzli…

Ofk` fnd`is


26/31

Ahst dkc chkhr advi d rhli fk `rdbt ibbfjdjy

Bftzpdtrfje it dl, Dktfhx. _ic. ?==6

• Jill ripldjinikt fk d @I ]C nhcil prhvfci dk hpphrtukfty th dssiss tai fnpdjt hb

nutdtfhks fk jill survfvdl

• @I Jill `rdbts jdk dlsh dllhw th dssiss dltirkdtfvis bhr pirshkdlfzic nicfjfki

• @I bhr C_IDCC dpprhdja th nhculdti F]ZJs-cirfvic chpdnfki kiurhks djtfvfty


27/31


NHCILZ SAI_D]FIZ


Dpplfjdtfhks hb @I



28/31

….hb aundks dkc khk aundk prfndtis

attp7//www.ouflcdoidr.jhn


29/31

Jhksfcirdtfhks OIBH_I dpplyfk` @I bhr KA] cfsidsi nhcilfk`

•

Cfsidsi-rildtic fssuis7 nutdtfhk,

dì hb hksit, synpthns

•

Zpijfis7 ìkhni, lfbispdk,

civilhpniktdl pdttirks,

ahusfk` kiics, oriicfk` cindkcs,

fnpdjt hb fkcfvfcudl ìkitfj

odje`rhukc (hutoric vs. fkoric)

•

Sistfk`7 spijfbfj th cfsidsi, dì,

spijfis

•

@I-rildtic fssuis7 nitahcs bhr pldsnfc

fkhjuldtfhk, ukfplix vs. nultfplix,rdkchn fksirtfhks/cilitfhks, hbb

tdrìt ibbijts, nhsdfjfsn Rirnflyid dkc Inohr`, G Kiurhsjf Nita ?=49

Eiip fk nfkc “wadt fb„ sjikdrfh dkc oi ridcy wfta jlfkfjdl

prfndti vitirfkdrfdk supphrt.


30/31

]rdjtfjis tadt ailp istdolfsa itafjdl odsfs bhr fkftfdtfk` bfrst-fk-aundk trfdls

Efnnilndk it dl, Nhv Cfs. ?==6

Jhkjirk ]rdjtfjis

Fktirkdl vdlfcfty D prfhrf ayphtaisfs stdtic

D prfhrf phwir jdljuldtfhks

_dkchnfzdtfhk

Olfkcic tridtnikt dllhjdtfhk

Olfkcic hutjhni dssissnikts

Cfsjussfhk hb nfssfk` cdtd

Ixtirkdl vdlfcfty Ixpldkdtfhk bhr silijtfhk hb dkfndl nhcil

Ixpldkdtfhk hb silijtfhk hb hutjhni nidsuris

Jhrrisphkcikji hb trfdl wfta prijlfkfjdljhkcftfhks

Hptfnfsn dkc Jrftfjdl rivfiw hb prijlfkfjdl cdtd

puolfjdtfhk ofds Bull puolfjdtfhk hb prijlfkfjdl stucfis


31/31

Zpijfdl Sadkes

Safs risidrja wds supphrtic oy `rdkts brhn tai YP FJS_,YP RJ_@I dkc tai KFA

Tuistfhks8 inohr`Mprfndti wfsj icu

YP - Ndcfshk7

Zjhtt Rirnflyid

Zjhtt @utarfiEdt Orduk

Sic @hlhs

Gikkd Erhpp

@ri` Pfipz

Nfjadil Niyir

Zu-Jauk Wadk`

Gdnfi Sahnshk

Kdkjy Zjaultz-Cdreik

Ghk Livfki

“Ouccy„ Jdpudkh

KFA7

Ndre Jhheshk

Jdrki``fi Nillhk

Dlix Lhkchk

Ndj@fll Ykfvirsfty7

Ghkdtadk Efnnilndk

reflections on genomic editing in nonhuman primates (marina emborg)

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