reduction 2020 technology update
TRANSCRIPT
Reduction 2020TechnologyUpdate
StefanTöpflDeutschesInstitutfürLebensmitteltechnike.V.
• Functionalization• Structure modification• Fermentation
Salt,fat and sugar reductionStrategies
Saltreduction
Example:Proteinfunctionalization bypressure
Reduction of salt content inarange of 1.2– 2.2%
Abb.1:Drip loss (%)of scalded sausage with varying salt content
• WHC– drip loss
èReducing salt contentresults inlower WHC
Lowsalt meat productsScalded sausage
Serotype 4b(3987/04)
p-T isokineticity diagrams for 5 log inactivation of 3 pathogenic strains of Listeria monocytogenes in “Black Forest Prosciutto” (Ham) after 15-240 s.
Serotype CLIP 74903Serotype CLIP 74902 1/2a
HighPressure ProcessingMicrobial inactivation
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Oligomeric Structure
Monomere Structure
Intermediate, melted state
Unfolding
Aggregation
0,1 MPa 100 – 200 MPa > 700 MPa300 – 500 MPa
Pressure treatmentImpactonproteins
Abb.3:(1)Control,(2)HPP100MPa– Saltaddition prior to HPP,(3)HPP400MPa– Saltaddition prior to HPP,(4)HPP100MPa– Saltaddition afterHPP,(5)HPP400MPa– Saltadddition afterHPP,(6)Saltand phosphateaddition (0,06%)
• Saltcontent 1.8%• 100 MPa
• 400 Mpa
• No phosphate (except 6)
Nr.2und6
• Good structure• Good WHC
1 2 3 4 5 6
Lowsalt meat productsHPPconditioning
Ø Declarationwiththeclaim“reducedinsalt”ispossible
Cooked sausage Cooked ham
NaCl 1.26% 1 % 1.33 % 1.1%Phosphate 0.25 % 0.25 % 0.25% 0.25%KCl - 0.26% - 0.2%Processconditions
- - 100MPaaftertumbling
100MPaaftertumbling
Finalsaltreduction
30% 45 % 30 % 42%
Lowsalt meat productsResults
Fat reduction
Example:Replacing fatby lean meat
HighPressure ProcessingHPPstructure modification
HPP treatment of lean meat (e.g. turkey breast or thigh)
• 600 MPa 3 min at ambient temperature
• Freezing
• Use to replace fat or lard in meat products
Benefits
• Reduction of fat content < 10 %
• No use of plant based oils or other animal types
• „Clean-Label“ – no gums, thickeners or fibers
DIL– HighProSticks•Proteincontent >50%(dependent onmoisture removal)
•Fatcontent<10%•Fitness,bodybuilders,kids …
ExampleformulationTurkeythigh70%Turkeybreast(600MPa,3min)30%Curingsalt2,0%Dextrose0,25%Pepper0,2%Startercultures
HighPressure ProcessingHPPstructure modification
Fat reduction
Example:Reducing fat indispersesystems
Example of a high pressure gap
Stansted Fluid Power HydLok
• pressure up to 400 MPa
• 2 – stage pressure system
• 100 l/h continuous flow rate
• 15 kW power consumption
• 8 bar pneumatic air supply
HighPressure HomogenizationEquipment
Structuralmodification
• Particlesizereduction(Nanoparticles,nanosuspensions)
• Modificationofconstituents(proteins,starchetc.)
Foodpreservation
• Reductionofmicrobialload• Enzymeinactivation
control 100 MPa
200 MPa 300 MPa
HighPressure HomogenizationEquipment
1. Preparation
- Water 56 %
- Egg yolk (PLA2 modified)* 8 %
- Sugar 2 %
- Mustard 1 %
- Salt 1 %
2. Oil addition and acidification
- Slow oil addition 28 %- Vinegar 3 %
- Citric acid 1 %
3. Homogenisation
- Variation of pressure level (100-350 MPa)
HighPressure HomogenizationLowfat mayonnaise
0,1 mm
0,1 mm 0,1 mm
APV (18/5 MPa)
300 MPa200 MPa
100 MPa
0,1 mm
300 MPa200 MPa
100 MPa
Light microscopyConfocal laser scanning microscopy (CLSM)
APV (18/5 MPa)
HighPressure HomogenizationLowfat mayonnaise
Flow rate: 360 l/h
Max. Pressure: 3800 bar (380 MPa)
Input Power: 56 kW
UHPHequipmentDILdevelopment
High pressure pump
Valves:
1st Stage und 2nd Stage
Fat reduction
Example:Reducing fat uptake duringfrying
PEFtreatment influences the structure of potatoes
Pulsed Electric FieldsStructure impact onfat uptake
Pulsed Electric FieldProcessingCutting improvement
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Productquality
• Texturesofteningandsmoothercut
• Higheryield(lessbreakage),Longerfries
• Reducedoiluptake(upto10%)
• Possibilitytodevelopnewcutsandshapes(e.g.Latticecut)
Processing
• Energyandwatersavings
• Increasedknifedurability(upto60%)
PEF
Oilcontent
5.7% 5.1%
Pulsed Electric FieldProcessingBenefits for FrenchFriesProcessors
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Productquality:• Reducedfat→smoothercut→countercurrentmasstransfer
• Crisperchip
PEF
OilcontentFryingtime:– 25%
Fastermoisturerelease→shorterfryingtime
0
2
4
6
8
10
12
14
16
160 180 200 220
Moisture%
Fryingtimeat175°C[s]
untreated PEF
Fryingtime:– 25%
0
2
4
6
8
10
12
14
16
160 180 200 220
Moisture%
Fryingtimeat175°C[s]
untreated PEF
1%
Fryingtime:– 25% 38.3% 25.5%
Pulsed Electric FieldProcessingPotato Snacks
-33 %
Equipment design
Sugarreduction
Example:Replacing sucrose by othersugars /sugar alcohols
SugarremovalBakery products
Pleasure Project, Le Bail, 2015
Dough rheology:Nativeegg(55%)foamed with Saccharoseor Fructose(45%)with addition of 25%wheat flour and 0,5%salt
SugarreplacementBakery products
SugarreplacementBakery products
DSC:Nativeegg(55%)foamed with Saccharoseor Fructose(45%)with additionof 25%wheat flour and 0,5%salt
Globuar proteins
native
Heat
Shear
unfolded denaturéd
60 – 70°C > 80°C
Stable due to
molecular interactions
Stabilized by covalent
bonds (-S-S-)
poss.
reversible
Instable, potential for
interactions and reactions
SugarreplacementProteindenaturation
Fructose SaccharoseSugar alcohol
Xylit
SugarreplacementBakery products
Colour (Melanoidins)
Volatiles, roasted and bitter flavours
Saccharose: non-reducing sugar
Maillard Reaction
Non-enzymatic
reaction of
sugars and
amino acids
SaccharoseFructose
!
SugarreplacementBrowning
Sugar alcohols
à No reducing sugar
à no Maillard-Reaction?!
Water removal during baking: 2
à increased reactivity
à dehydration
à Maillard-Reaktion
Slow reaction with complex
reaction paths
Sugar alcohol
Xylit
Xylit
SugarreplacementBrowning
25°C 35°C 45°C 55°C
65°C 75°C 85°C 95°C
Dough with Saccharose
SugarreplacementBaking
25°C 35°C 45°C 55°C
65°C 75°C 85°C 95°C
Dough with Fructose
SugarreplacementBaking
Dough with
Fructose
25°C 95°C
Dough with
Saccharose
SugarreplacementBaking
Sugarreduction
Example:Reducing sugar byfermentation
SugarreplacementEnzymatic hydrolysis
Pleasure Project, Le Bail, 2015
SugarreplacementEnzymatic hydrolysis
Pleasure Project, Le Bail, 2015
SugarremovalFruit products
In purees up to 30 % reduction reached, no significant change in fruit ingredients.
Pleasure Project, Lötzbeyer, 2015
SugarremovalFruit products
Pleasure Project, Lötzbeyer, 2015
SugarremovalFruit products
Pleasure Project, Lötzbeyer, 2015
Biotechnological approach for sugar reduction processes, other reaction
pathways e.g. gluconic acids possible as well
Structure design
Example:Snackand highmoistureextrusion
Extrusion
65 % protein concentrate (soy, pea, dairy, algae…)
35 % waterFat free impingement or mold baking
Targeted processing allows structure modification and functionalityenhancement:
• Pressure induced protein unfolding can increase water binding capacity andallow partialreplacement of salt
• Reduction of lipid droplet size by ultra highpressure homogenization allowsviscosity increase and reduction fat content
• Structure modification of planttissue results incutting improvement andlower fat uptake
• Sugarreduction can be achieved by replacement or fermentation
• Extrusionallows designof low fat carbohydrate and protein based structures
Conclusions