rb biochemistry - center for cancer researchredox biology: biochemistry david a. wink and murali c....

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Page 1: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

RB biochemistry

Page 2: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

Biochemistry Summary

Page 3: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

Formation and Abatement of Oxidants

Page 4: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

Oxygen Reduction

Page 5: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

Redox Cycling

Page 6: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

Menadione and Dopamine

Page 7: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

Pro-Oxidant vs Antioxidant

Page 8: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

Vitamins Working in Concert

Page 9: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

Xenobiotic Metabolism

Page 10: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

Mitochondrial Sources of ROS

Page 11: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

Energetics of ROS Formation

Page 12: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

Inner-Sphere Reduction Examples

Page 13: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

P450 System

Page 14: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

Enzymatic Generation Purpose

Page 15: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

Hydrogen Peroxide Formation Example

Page 16: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

Xanthine Oxidase

Page 17: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

Xanthine Oxidase

Page 18: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

NADPH Oxidase

Page 19: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

NADPH Oxidase

Page 20: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

Nitric Oxide Synthase

Page 21: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

Nitric Oxide Synthase

Page 22: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

NOS Biochemistry

Page 23: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

Heme Oxygenase

Page 24: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

Biochemistry of Prevention

Page 25: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

Biochemistry Reactive Oxygen Species

Page 26: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

Preventive Mechanisms

Page 27: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

Chemical Biology of Nitric Oxide

Page 28: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

Scavenging of NO

Page 29: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

Glutathione Peroxidase Biochemistry

Page 30: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

Peroxiredoxin

Page 31: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

Seleno-Cysteine Reduction

Page 32: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

Glutathione Metabolism

Page 33: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

Abatement of Nitrosative Stress

Page 34: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

Glutathione and Ascorbate Pools

Page 35: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

Urate and Uric Acid

Page 36: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

Interaction of NO and ROS

Page 37: RB biochemistry - Center for Cancer ResearchRedox Biology: Biochemistry David A. Wink and Murali C. Krishna National Institutes of Health National Cancer Institute Radiation Biology

Oxygen and NAPDH Essential