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Rashtreeya Sikshana Samithi Trust

R.V. College of Engineering (Autonomous Institution affiliated to VTU, Belagavi)

Department of Biotechnology Master of Technology (M. Tech.)

Biotechnology

Scheme and Syllabus of

Autonomous System w.e.f 2016

Department of Biotechnology M. Tech – Master of Biotechnology

Scheme and Syllabus – 2016 Admission Batch of 47

R.V. College of Engineering, Bengaluru – 59 (Autonomous Institution affiliated to VTU, Belagavi )

Department of Biotechnology

Vision:

A premier department in Biotechnology Education, Research and Innovation with a

focus on sustainable technologies for the benefit of society and environment.

Mission:

• Create state-of-the-art infrastructure for research and training in Biotechnology.

• Develop graduates who are ethical and socially concerned.

• Promoting collaboration with academia, industries and research organizations at

National and International level.

• Contribute to socioeconomic development through sustainable and inclusive

technologies.

Program Educational Objectives (PEO)

M. Tech. in Biotechnology Post Graduates will

PEO1: Have an ability to solve Biotechnology and Engineering problems, communication skills

and team work that prepare them for a successful career in Biotechnology and allied

industries.

PEO2: Function at a technically competent level in formulating and solving problems in

Biotechnology and develop an outlook for higher education and lifelong learning.

PEO3: Gain the ability to comprehend, analyze,design and develop biological processes and

products, exhibit professionalism, ethical attitude to become an entrepreneur.

Program Specific Criteria (PSC)

Lead Society: American Society of Agricultural and Biological Engineers

PSC1: The curriculum of program consists of basics of Biotechnology, Chemical engineering

and informatics knowledge for Biological processes. The curriculum must prepare

students to analyze and develop techniques or methods to solve

PSC2: The curriculum of program includes advance core and electives courses together with

laboratory, seminar, industry visit, assignment and tutorials emphasizing on production,

processing, and management of biological, agricultural, food, Health and natural

resources.

PSC3: The curriculum of program also includes internship and project work which prepare

students to apply biotechnological solutions and professional skills with focus on

production, management and research.



Program Objectives (POs)

PO 1. Scholarship of Knowledge: Use knowledge of principles and applications of

Biotechnology.

PO 2. Critical Thinking: Design and formulate the solutions for various biotechnological

challenges.

PO 3. Problem Solving: Develop methodologies to solve the problems in the fields of health &

pharma, bioprocess, and food & Agriculture.

PO 4. Research Skill: Possess skills appropriate to conduct research in life sciences with

minimal supervision.

PO 5. Usage of modern tools: Apply advanced tools and techniques to the challenges of

biotechnological sector.

PO 6. Collaborative and Multidisciplinary work: Collaborate with the confluence of various

domains of Biotech from academic, industry and research institutes of national or

international repute.

PO 7. Project Management and Finance: Design and develop projects related to

biotechnological and allied branches keeping performance and cost constraints into

consideration.

PO 8. Communication: Inculcate inter and intra personal skills for effective communication.

PO 9. Life-long Learning: Appreciate the breadth and depth of knowledge in biotech and the

necessity for lifelong learning.

PO 10. Ethical Practices and Social Responsibility: Apply bio-engineering solutions to

societal and ethical needs with focus on sustainability.

PO 11. Independent and Reflective Learning: Contemplate on real-time problems and develop

independent interpretations.

Program Specific Outcomes (PSO)

PSO1: The post graduates of the program will be thorough with biotechnology, chemical

engineering and statistics to deal with the Engineering problems related to Biotechnology

and Bioinformatics.

PSO2: The post graduates of the program will be able to deal with chemical engineering and

Biotechnology problems related to upstream and downstream process technology as well

as will be ready to solve societal problems ethically.

PSO3: The post graduates of the program will be able to apply biotechnological tools in

Agriculture, health sector and Fermentation industry with emphasis on production,

management, entrepreneurship and research.



R. V. College of Engineering, Bengaluru – 59. (An Autonomous Institution affiliated to VTU, Belagavi)

. Department of Biotechnology

M. Tech. in Biotechnology

FIRST SEMESTER

Sl.

No

Course Code Course Title BoS CREDIT ALLOCATION Total

Credits Lecture

L

Tutorial

T

Practical

P

Self Study

S

1 16MEM11P Project Management IM 3 1 0 0 4

2 16MBT12 Advanced Biostatistics. BT 4 0 0 0 4

3 16MBT13 Recombinant DNA Technology

(Theory and Practice)

BT 4 0 1 0 5

4 16MBT14 Computational Biology. BT 4 0 0 1 5

5 16MBT15X Elective-1 BT 4 0 0 0 4

6 16HSS16 Professional Skill Development BT 0 0 2 0 2

Total 19 1 3 1 24

Elective -1

16MBT151 Genomics, Proteomics and Microarray. 16MBT152 Enzyme Technology.






SECOND SEMESTER

Sl.

No


Credits Lecture

L

Tutorial

T

Practical

P

Self Study

S

1 16MEM21R Research Methodology IM 3 1 0 0 4

2 16MBT22 Upstream Processing


BT 4 0 1 0 5




6 16MBT26 Minor Project BT 0 0 5 0 5

Total 19 1 6 0 26

Elective -2

16MBT231 Immunotechnology. 16MBT232 Nanobiotechnology.

Elective – 3

16MBT241 Pharmaceutical Biotechnology. 16MBT242 Agricultural Biotechnology.

Elective – 4

16MBT251 Applications of Recombinant DNA Technology 16MBT252 Bioreactor Engineering




Department of Biotechnology


THIRD SEMESTER

Sl.

No


Credits Lecture

L

Tutorial

T

Practical

P

Self Study

S

1 16MBT31 Downstream Processing

& Product Recovery


BT 4 0 1 0 5




5 16MBT35 Internship / Industrial

Training

BT 0 0 3 0 3

6 16MBT36 Technical Seminar BT 0 0 2 0 2

Total 16 0 6 0 22

Elective -5

16MBT321 Stem Cell and Tissue Engineering. 16MBT322 Food Technology.

Elective – 6

16MBT331 Human Diseases. 16MBT332 Process modelling & simulation

Elective-7

16MBT341 Regulatory Affairs & Biobusiness. 16MBT342 Medical Devices.






FOURTH SEMESTER

Sl.

No

Course

Code

Course Title BoS CREDIT ALLOCATION Total

Credits Lecture

L

Tutorial

T

Practical

P

Self Study

S

1 16MBT41 Major Project BT 0 0 26 0 26

2 16MBT42 Seminar BT 0 0 2 0 2

Total 0 0 28 0 28



FIRST SEMESTER ADVANCED BIOSTATISTICS

Course Code : 16MBT12 CIE Marks : 100

Hrs/Week : L:T:P:S:: 4:0:0:0 SEE Marks : 100

Credits : 4 SEE Duration : 3 Hrs

Course Learning Objectives (CLO):

Students are able to:

1. Understand the fundamentals of biostatistics

2. Address the relevance of probability and statistics to advanced engineering problems in

biotechnology.

3. Apply more advanced techniques to analyze the data and modeling the complex systems.

4. Design the biological experiments and its analysis based on the real data.

Unit – I 09Hrs

Fundamentals of Statistics: Introduction to statistics, Measurement of data scale and Central

tendency, Measures of dispersion: range, percentile, variance and standard deviation. Data handling

and statistical variables. Characteristics of biological data, Elementary theory of statistical errors.

Continuous random variables-normal distribution, discrete random variables-Binomial and poisons

distribution. Logarithmic transformations. Application of statistics to biological problems and their

interpretation.

Unit – II 9Hrs

Inferential Statistics : Basics of experimental design, Random block design, stratified design; cohort

studies, case-control studies, and odd ratio. Principles of statistical inference: Parameter estimation,

hypothesis testing. Statistical inference on categorical variables; categorical data and Single- and

Double-blind experiments; Sampling distributions: Bivariate distribution-conditional and marginal

distribution-Discrete distribution-Binomial, Poisson, geometric distribution-Continuous distribution,

Normal, simple problems-properties.

Unit – III 10Hrs

Hypothesis Testing: Null and alternative hypotheses, decision criteria, critical values, type I and type

II errors, Meaning of statistical significance; Power of a test; One sample hypothesis testing:

Normally distributed data: z, t and chi-square tests; F-tests. Binomial proportion testing. Independent

and dependent sample comparison, Wilcoxon Signed Rank Test, Wilcoxon-Mann-Whitney Test,

Kruskal-Wallis test and Analysis of variance: One-way and Two-way Tables.

Unit – IV 10Hrs

Statistical Curve Fitting: Correlation-Correlation coefficient, properties-problems on Karl Pearson

and Spearman Rank correlation coefficient, Rank correlation-Regression equations problems-curve

fitting by the method of least squares-fitting curves of the form ax+b,ax2 +bx+c,abx and axb -

Bivariate correlation application to biological problems. Regression: simple linear regression; Least

squares method; Multiple linear regression model, Optimization strategies with case studies.

Unit – V

Predictive tools in Statistics: Overview of SAS, SAS data sets, SAS functions, Data

Management -sorting and grouping report data, Statistical Analysis-Algorithm and

implementation using numerical methods with case studies using β-version. MINITAB:

Introduction, Accessing Minitab, worksheet menu and session commands, data entering

10Hrs



and analyzing the simple formula for obtaining statistical inference. Application of

computer for analysis, interpretation and validation of statistical data.

COURSE OUTCOMES

After going through this course the student will be able to

1. Organize data graphically and numerically, analyze and interpret/conclude the outcome of the

biological experiments.

2. Develop technical competence in designing research studies and analyzing data pertaining to the

biotechnological context.

3. Compare the utility of multivariate statistical methods in transcultural health research

4. Analyze real-life datasets using statistical predictive techniques and tools.

REFERENCE BOOKS:

1. Wayne W. Daniel , Chad L. Cross., “Biostatistics : Basic Concepts and Methodology for the

Health Sciences” Wiley India Pvt Ltd. 10th Edition, 2014. ISBN: 9788126551897.

2. Shalabh Helge Toutenburg., “Statistical Analysis of Designed Experiments”, Wiley Publication,

Third Edition, 2010. ISBN-13: 978-1441911476

3. Arora P.N and Malhan P.K. “Biostatistics”, Himalaya Publishing House, 2013 ISBN: 81-8318-

691-2.

4. Pullaiah T., Ravindra Reddy B., Sarma K. L. A. P., “Biostatistics”., Daya Publishing House,2013:

ISBN: 9788170359234.

Scheme of Continuous Internal Evaluation (CIE):

CIE will consist of TWO Tests, TWO Quizzes and ONE assignment. The test will be for 30 marks

each and the quiz for 10 marks each. The assignment will be for 20 marks. The total marks for CIE

(Theory) will be 100 marks.

Scheme of Semester End Examination (SEE):

The question paper will have FIVE questions with internal choice from each unit. Each question will

carry 20 marks. Student will have to answer one question from each unit. The total marks for SEE


Mapping of COs with POs

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 CO1 H L H - - - - - - - - CO2 H M H - - - - - - - - CO3 L H H - - - - - - - - CO4 L H H - - - - - - - -

Mapping of COs with PSOs

PSO1 PSO2 PSO3

CO1 H L L

CO2 H H L

CO3 L H L

CO4 M M L



RECOMBINANT DNA TECHNOLOGY


Course Code : 16MBT13 CIE Marks : 100+50

Hrs/Week : L:T:P:S 4:0:1:0 SEE Marks : 100+50

Credits : 5 SEE Duration : 3 + 3hrs



1. Acquire knowledge of central dogma of molecular biology and rDNA technology.

2. Study the techniques of Recombinant DNA technology.

3. Acquire the various methods of genetic transformation of living systems, and selection,

screening and analysis of recombinants.

4. Know various advanced techniques of genetic manipulation of microbes, plants and animals.

Unit – I 09 Hrs

Central Dogma of Molecular Biology: Molecular structure of genes and chromosomes, Replication,

transcription and translation in prokaryotes and eukaryotes. Gene regulation: Gene regulation and

Operon concept, Constitutive, Inducible and Repressible systems; Operators and Regulatory

elements; Positive and negative regulation of operon: lac, trp, ara, his, and gal. Promoters and

enhancers, Structure and function of different types of RNA and mRNPs. Regulation of Translation:

global vs mRNA-specific. Translation inhibitors, Posttranslational modifications of proteins. Protein

trafficking and transport.

Unit – II 10 Hrs

Components of rDNA technology: Isolation and purification of DNA (genomic and plasmid) and

RNA. Chemical synthesis of DNA: Phosphoramidite method, use of synthesized oligonucleotides.

Labelling nucleic acids: Radioactive and non-radioactive, end labeling, nick translation, primer

extension. Nucleic acid hybridization, Gel electrophoresis. Restriction enzymes, DNA modifying

enzymes (Nucleases, Polymerases), DNA ligases. Host cells: Prokaryotic and eukaryotic hosts.

Vectors: plasmid, bacteriophage and other viral vectors, cosmids, Ti plasmid, Ri plasmids, Yeast

Episomal Plasmids (YEPs), Yeast integrative plasmids (Yips), Yeast replicative plasmids, Yeast

Artificial Chromosome (YAC), mammalian and plant expression vectors, Gate-way vectors.

Unit – III 10 Hrs

Genetic Transformation and Cloning strategies: Transformation and transfection, Packaging phage

DNA in vitro, Alternative DNA deliver methods: Electroporation, microinjection, biolistic. Cloning

from mRNA: synthesis if cDNA, cloning cDNA in plasmid vectors, cloning cDNA in bacteriophage

vectors. Cloning from genomic DNA: Genomic libraries, preparation of DNA fragments for cloning,

ligation, packaging, and amplification of libraries. Advanced cloning strategies: synthesis and cloning

of cDNA, Expression of cloned DNA molecules, Cloning large DNA fragments in BAC and YAC

vectors.

Unit – IV 9 Hrs

Selection, Screening, and analysis of Recombinants: Genetic selection and screening methods:

Using chromogenic substrates, Insertional inactivation, Complementation of defined mutation, other

genetic selection methods. Screening using nucleic acid hybridization: Nucleic acid probes, Screening

clone banks. Screening using PCR, Immunological screening for expressed genes. Analysis of cloned

genes: Characterization based on mRNA translation in vitro, Restriction mapping, Blotting

techniques, DNA sequencing.



Unit – V 10 Hrs

The Applications of rDNA technology: Production of proteins: Native and fusion proteins, Yeast

expression systems, Baculovirus expression system, mammalian cell lines. Protein engineering:

Rational design, Directed evolution. RNAi technology: si RNA and miRNA mediated gene silencing,

antisense technology. Genome editing: Clustered regularly interspaced short palindromic repeats

(CRISPR)/Cas systems, Zinc finger nucleases, Transcription activator-like effector nuclease

(TALENS). Applications of synthetic Riboswitches, Identification of genes responsible for human

diseases. Gene therapy, DNA profiling, Transgenic plants and animals. Ethical and regulatory issues.

Unit – VI (Lab Component)

1. Isolation and purification of genomic DNA from prokaryotic/ eukaryotic cells

2. Isolation and purification of plasmid DNA

3. Isolation and purification of total RNA

4. Restriction digestion of DNA

5. Constructing recombinant DNA using gene of interest and vector

6. Preparation of competent cells of E.coli and genetic transformation of E.coli

7. Blue and white screening of transformants, confirmation of transformants using colony PCR

8. Agrobacterium mediated genetic transformation of plants

9. Amplification of DNA fragments using PCR

10. Extraction of proteins from prokaryotic/ eukaryotic cells.

Expected Course Outcomes:

After going through this course the student will be able to:

CO1: Explain the mechanism of DNA replication, transcription, translation, gene regulation and

recombinant DNA technology.

CO2: Apply recombinant DNA technology for genetic manipulation of prokaryotes and eukaryotes.

CO3: Analyze and evaluate recombinant proteins/compounds, and genetically modified organisms.

CO4: Design/develop suitable protocol/technique for production of genetically modified organisms or

heterologous proteins/compounds in living system.

Reference Books:

1.

Nicholl DST., An introduction to Genetic Engineering, Cambridge, 3rd edition, 2010,

ISBN:978-0-521-61521-1

2.

Glick BR, Pasternak JJ, and Patten CL, Molecular Biotechnology – Principles and applications

of recombinant DNA, ASM Press, 4th Edition. 2010. ISBN:978-1-55581-498-4.

3. Brown TA. Gene Cloning and DNA Analysis – An Introduction, Wiley-Blackwell Science, 6th

Edition, 2010, ISBN: 9781405181730.

4.

Lodish H, Berk A, Kaiser CA, Krieger M, Scott MP, Bretscher A, Ploegh H and Matsudaira P,

Molecular Cell Biology, Freeman, 8th Edition, 2016, ISBN-13: 978-1464183393.











Scheme of Continuous Internal Evaluation (CIE) for Lab:

CIE for the practical courses will be based on the performance of the student in the laboratory, every

week. The laboratory records will be evaluated for 40 marks. One test will be conducted for 10

marks. The total marks for CIE (Practical) will be for 50 marks.

Scheme of Semester End Evaluation (SEE) for Lab:

SEE for the practical courses will be based on conducting the experiments and proper results for 40

marks and 10 marks for viva-voce. The total marks for SEE (Practical) will be 50 marks.

Mapping of COs with POs PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11

CO1 M M L - - - - L L - -

CO2 H H M M M - - L L - -

CO3 H H M M M - - L L - -

CO4 H H H M H - - L L - -


PSO1 PSO2 PSO3

CO1 L L L

CO2 H M M

CO3 H M L

CO4 H H H



COMPUTATIONAL BIOLOGY


Hrs/Week : L:T:P:S 4:0:0:1 SEE Marks : 100

Credits : 5 SEE Duration : 3 hrs



1. Understand the importance of Information Technology and Stochastic as well as Numerical

approaches to solve the problems related to High Throughput Biological Data

2. Acquire knowledge of Platforms and Workflow of Big Data Analytics with case studies

3. Apply Computational tools and techniques to model and simulate cellular phenomenon

4. Explore practical applications of Computational Biology in Genomics, Proteomics, Cancer

Biology and Immunology

Unit – I 10Hrs

Statistical approach to DNA and Protein sequence analysis: Introduction, scope and applications

of Computational biology. Molecular Biology databases. Analysis of single DNA sequence: shotgun

sequencing, DNA modeling, Scanning long repeats, Analysis of patterns and Counting of overlaps.

Analysis of Multiple DNA or Protein sequences: Frequency comparisons of two sequences. Simple

tests for significant similarity in an alignment. Alignment algorithms for two sequences: Gapped

global comparisons and Dynamic programming algorithms, linear gap model for fitting one sequence

into another and local alignment.

Unit – II 08Hrs

High Throughput Data Analysis: Introduction Next Generation Sequencing(NGS), NGS

Experimental Work Flow, Scope and Applications. NGS Platforms - Illumina Reverse Dye-

Terminator, Ion Torrent Semiconductor sequencing and Pacific Biosciences Single Molecule Real-

Time Sequencing. NGS Data Analysis; Base calling and quality score, Data Quality Control and

Preprocessing, Reads Mapping – Mapping approaches and algorithms, and Tertiary analysis.

Computing Needs for NGS – Data storage, transfer, Computing power, Software needs and

Bioinformatics Skills. Case study – Genotyping and Genomics Variation Discovery by Whole

Genome resequencing.

Unit – III 09Hrs

Molecular Modeling and Simulation: Introduction to Molecular Modeling and Simulation; brief

introduction to protein structure hierarchy, structural databases, and Force fields. Modeling

applications – prediction of secondary structure of Protein and RNA, Prediction of 3D structure of

Proteins, Prediction of Binding pockets, pocket analysis and Molecular docking – algorithms

involved. Application of Simulation – Modeling and simulation of Permeation events, membrane

dynamics and protein dynamics. Case studies of modeling and simulation – simulation of Water

permeation and Fluidic nature of membrane.

Unit – IV 10Hrs

Computational Biology and Cancer research: Mathematical modeling of tumorigenesis - Cellular

automaton, tumor, angiogenesis. One hit and two hit stochastic models - Tumor suppressor gene,

Kolmogorov forward equation, and retinoblastoma. Microsatellite and chromosomal instability in

sporadic - APC gene, colorectal cancer, point mutation. Chromosome loss. Basic models of tumor

inhibition and promotion - Metastatic, Angiogenic tumor cells, Angiogenesis inhibition. Mechanisms

of tumor neovascularization - vasculogenesis, Cancer and Immune responses - Dendritic cell



vaccination, Viruses as antitumor weapons - Tumor load, Viral replication and Oncolytic viruses.

Unit – V 10Hrs

Computational Immunology: Overview of immune system,Introduction to computational

immunology Immunological databases – IMGT – IMGT-GENE-DB, – IMGT-HLA, Tools for the

prediction binding affinity between peptide : TAP:MHC:TCR- MHC: Peptide Binding Prediction -

SYFPEITHI, BIMAS, MHC PRED, - Future of computational modeling and prediction systems in

clinical immunology -overview of models- models for HIV infection.



CO1: Explain conceptually Sequence, Protein Structure Hierarchy, Physical and Virtual mapping of

Biological data

CO2: Apply computational tools and techniques to solve problems in the field of Proteomics,

Genomics, Cancer biology as well as Immunology

CO3: Analyze and evaluate High Throughput Data generated by sequencing/mapping/hybridization

and other projects using Clustering and searching algorithms with case studies

CO4: Design and execute protocols to perform high throughput data analysis in the field of

Proteomics, Genomics, Cancer biology as well as Immunology

Reference Books:

1. Wang X, Next-Generation Sequencing Data Analysis, CRC Press, 2016, ISBN 9781482217896

2. Schlick T, Molecular Modeling and Simulation: An interdisciplinary discipline, Springer, 2nd

edition, 2010, ISBN 9781441963505

3. Flower D, Timmis J. In Silico Immunology, Springer Link, 2007, ISBN: 978-0-387-39238-7

4. Wodarz D, Komarova N. Computational Biology of Cancer: Lecture Notes and Mathematical

Modeling, World Scientific, 2005, ISBN 9789814481878









Mapping of COs with POs and PSOs PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11

CO1 H L L L L M - - - - -

CO2 M L M M H H - - L L L

CO3 M H H L H M - - M M -

CO4 M H H M M M - - M - M


https://www.google.co.in/search?tbo=p&tbm=bks&q=inauthor:%22Xinkun+Wang%22



PSO1

PSO2

PSO3

CO1 L M H

CO2 L H H

CO3 L L L

CO4

H H L



GENOMICS, PROTEOMICS AND MICROARRAY

(Elective-1)






1. Have a basic understanding of the organization of prokaryotic and eukaryotic genomes,

databases and sequencing techniques

2. Acquire molecular insight into the tools and techniques used in genome analysis.

3. Get an overview of the different protein purification and sequencing techniques

4. Get insight into the techniques involved in the identification and characterization of

all the proteins synthesized in a cell or a tissue.

5. Understand the basic principles of DNA and Protein microarray

Unit – I 10 Hrs

Introduction to Genomics: Genome evolution and organization in prokaryotes and eukaryotes,

Genome mapping: Genetic and physical mapping. Molecular markers and protein markers,

Genome sequencing, basics, strategies and methodology. Comparative and Functional genomics;

Model systems- Arabidopsis, Human, Drophila and E coli. Serial analysis of gene expression

(SAGE) and targeting induced local lesions in genome (TILLING).Biological databases; Primary

and secondary for nucleic acid and proteins, structural databases, metabolic pathways and

specialized databases. Genome Wide Association Studies (GWAS)

Unit – II 10Hrs

Tools for genomics ; Computational analysis of sequences- finding genes and regulatory regions;

Gene annotation; Similarity searches; Pairwise and multiple alignments; Alignment statistics;

Prediction of gene function using homology, context, structures. Expression sequence tags (ESTs),

Microarrays technology- Principles and applications, FISH, transcriptome analysis and SNPs

determination. Allele mining and single nucteotide polymorphisms (SNPs).Transcriptomics;

Cancer Genomics, Epigenomics, Chemical Genomics; Metabolomics, Nutrigenomics,

interactomics, Metagenomics. Personal Genomics; Social, Legal and Ethical Implications of

Human Genome Research.

Unit – III 9Hrs

Introduction and scope of proteomics, Protein separation techniques: Ion exchange, Size exclusion

and affinity chromatographic techniques, Poly acrylamide gel electrophoresis, isoelectric focusing,

two dimensional poly acrylamide gel electrophoresis, Mass spectrometry based techniques for

protein identification.

Unit – IV 10Hrs

Protein sequencing- Edman degradation, mass fingerprinting, protein synthesis and post

translational modifications. Identification of phosphorylated proteins, characterization of multi-

protein complexes, protein - protein interactions and quantitative proteomics- Characterization of

interaction clusters using two-hybrid systems. Protein arrays-definition, applications- diagnostics,

expression profiling, Functional proteomics, Protein structure analysis, Protein databases, Clinical

and biomedical applications of proteomics.



Unit – V 09Hrs

Microarray techniques: Importance and applications of microarray techniques in biotechnology,

Types – Single and multiple approaches. Challenges of microarray technology. Microarray Probe

preparation, hybridization, Image processing, Transformation of expression ratio and data

normalization. Low and high level information Analysis – Data Preprocess of Chemical

compounds Microarray, Biomolecular microarray - Protein and proteomics Microarray, DNA

Microarray, MicroRNA Population, Cellular and tissue microarray. Microarray Database for Serial

Analysis of Gene Expression, Gene Expression Omnibus of NCBI, Array Express of EMBL and

Antibodies Arrays on Miniature Western Blots methods.



CO1: Explain the construction concepts of various genome maps and large scale sequencing

CO2: Apply diagnostic tools for plant, animal and human diseases

CO3: Analyse proteomics to solve complex biological problems regardless of types of organism.

CO4: Develop the basic concepts of microarrays and analyze the differential gene expression.

Reference Books:

1. Sandor Suhai, Genomics and Proteomics: Functional and Computational aspects, Kluwer

academic publishers, 2007, ISBN: 9780306468230

2. Liebler,D.C. Introduction to Proteomics: Tools for the New Biology, Humana Press,

2002. ISBN-13: 978-0896039926

3

R.M. Twyman, Principles of Proteomics, garland Science/BIOS Scientific publishers, 2004,

ISBN-10: 1-85996-273-4.

4

Steven Russell, Lisa A. Meadows and Roslin R. Russell. Microarray Technology in Practice:

2nd Edition, Academic Press, 2013. ISBN 13: 978-0-12-372516-5



each and the quiz for 10 marks each. The assignment will be for 20 marks. The total marks for

CIE (Theory) will be 100 marks.


The question paper will have FIVE questions with internal choice from each unit. Each question

will carry 20 marks. Student will have to answer one question from each unit. The total marks for

SEE (Theory) will be 100 marks.


PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11

CO1 H M M L L - - M M L L

CO2 H H H H M - - M M L L

CO3 H H H H M - - M M L L

CO4 H M M L L - - M M L L

Mapping of COs with PSOs PSO1 PSO2 PSO3

CO1 L M L

CO2 M M M

CO3 M M M

CO4 L M L



ENZYME TECHNOLOGY

(Elective-1)






1. Get an overview of isolation, purification and assay of enzymes of commercial

importance.

2. Learn the properties of enzymes, enzyme kinetics and enzyme inhibitors and their

practical application.

3. Understand the techniques of enzyme immobilization and its utility in therapeutics,

analytics and manufacturing applications

4. Get an insight about the industrial application of enzymes and the challenges ahead.

Unit – I 8 Hrs

Introduction- Nomenclature and classification, Application of enzymes in process industries and

health care. Enzymes as biocatalysts: advantages and disadvantages over chemical catalysts and

characteristics, microbial production and purification of enzymes, Assay of enzyme activity and

specific activity, Coenzymes, Cofactors. Bioreactors for enzyme catalyzed reactions.

Unit – II 12Hrs

Enzyme-Ligand interaction, collision theory and transition state theory and role of entropy in

catalysis, Methods for investigating the kinetics of Enzyme catalyzed reactions – Initial velocity

studies, End point, Steady state and Pre steady state Kinetics, Estimation of Michaelis-Menten

parameters, kinetics of single and multisubstrate enzymes catalyzed reactions (Ping-Pong

mechanism and King and Altman procedure). Allostericity and cooperativity. Effect of pH and

temperature on enzyme activity. Numerical in enzyme kinetics.

Unit – III 10Hrs

Enzyme inhibition- irreversible and reversible competitive, noncompetitive, uncompetitive, mixed,

inhibitions. Kinetic differentiation and graphical methods. Examples. Determination of inhibitor

constant, therapeutic, diagnostic and industrial applications of enzyme inhibitors. Numerical in

enzyme inhibition.

Unit – IV 10Hrs

Techniques of enzyme immobilization; kinetics of immobilized enzymes, effect of solute, activity

& kinetics of immobilized enzymes; applications of immobilized enzyme technology-: Enzyme

sensors for clinical analysis, therapeutic medicine, Environmental applications. Economic

argument for immobilization. Case studies on therapeutic enzymes.

Unit – V 8Hrs

Industrial Applications of Enzyme Technology: Textile industry, detergents, pulp and paper,

leather, wood, animal feed, food and dairy industry - amylases, proteases, lipases, pectinases.

Enzyme Engineering: Glucose isomerase, subtilisin, redesigned lactate dehydrogenase. Synthetic



enzymes- peroxidase. Catalytic antibodies. Challenges in enzyme technology.

Course Outcomes:


CO 1. Develop strategy for isolation, its purification and measure its activity.

CO 2. Apply the enzyme and inhibition kinetics to problems and find the optimum values for

parameters affecting enzyme activity.

CO 3. Analyze and find the best method of immobilizing enzymes that can solve the therapeutic

challenge.

CO 4. Predict the enzymes to suit a given job or modify / customize the enzyme properties

required for industrial application.

Reference Books:

1. Klaus Buchholz, Volker Kasche and Uwe Theo Bornscheuer. Biocatalysis and Enzyme

Technology. 1st edn. Wiley-VCH, 2005.

2. Wolfgang Aehle. Enzymes in industry-production and applications. 3rd edn. Wiley-VCH,

2007.

3. Krishna Prasad N., Enzyme Technology: Pacemakers of Biotechnology, 1st ed., PHI learning,

New Delhi, ISBN:978-81-203-4239-2

4. Price N. C. and L Stevens. Fundaments of Enzymology: 3rd edn. Oxford University Press.

2003.











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PROFESSIONAL SKILL DEVELOPMENT

Course Code : 16HSS16 CIE Marks : 50

Hrs/Week : L:T:P:S 0:0:4:0 Credits : 02



1. Understand the importance of verbal and written communication

2. Improve qualitative and quantitative problem solving skills

3. Apply critical and logical think process to specific problems

4. Manage stress by applying stress management skills

UNIT 1 5 hours

Communication Skills: Basics of Communication, Personal Skills & Presentation Skills,

Attitudinal Development, Self Confidence, SWOC analysis.

Resume Writing: Understanding the basic essentials for a resume, Resume writing tips

Guidelines for better presentation of facts.

UNIT 2 6 hours

Quantitative Aptitude and Data Analysis: Number Systems, Math Vocabulary, fraction

decimals, digit places etc. Reasoning and Logical Aptitude, - Introduction to puzzle and games

organizing information, parts of an argument, common flaws, arguments and assumptions.

Verbal Analogies – introduction to different question types – analogies, sentence completions,

sentence corrections, antonyms/synonyms, vocabulary building etc. Reading Comprehension,

Problem Solving UNIT 3 4 hours

Interview Skills: Questions asked & how to handle them, Body language in interview,

Etiquette, Dress code in interview, Behavioral and technical interviews, Mock interviews -

Mock interviews with different Panels. Practice on Stress Interviews, Technical Interviews,

General HR interviews

UNIT 4 5 hours

Interpersonal and Managerial Skills: Optimal co-existence, cultural sensitivity, gender

sensitivity; capability and maturity model, decision making ability and analysis for brain

storming; Group discussion and presentation skills;

UNIT 5 4 hours

Motivation and Stress Management: Self motivation, group motivation, leadership abilities

Stress clauses and stress busters to handle stress and de-stress; professional ethics, values to be

practiced, standards and codes to be adopted as professional engineers in the society for

various projects.

Note: The respective departments should discuss case studies and standards pertaining to their

domain

Course Outcome:

The Graduate will be able to :

CO1: Develop professional skill to suit the industry requirement

CO2: Analyze problems using quantitative and reasoning skills

CO3: Develop leadership and interpersonal working skills

CO4: Demonstrate verbal communication skills with appropriate body language.



Reference Books:

Scheme of Continuous Internal Examination (CIE)

Evaluation will be carried out in TWO Phases.

Phase Activity Weightage

I After 5 weeks - Unit 1, 2 & Part of Unit 3 50%

II After 10 weeks – Unit 3, 4, 5 50%

CIE Evaluation shall be done with weightage as follows:

1. Writing skills 10%

2. Logical Thinking 25%

3. Verbal Communication & Body Language 35%

4. Leadership, Interpersonal and Stress Bursting Skills 30%

Mapping of Course Outcomes (CO) to Program Outcomes (PO)


CO1 - - - - - - - H H M H

CO2 - - - - - - - H H M H

CO3 - - - - - - - H H H H

CO4 - - - - - - - H H H h

Mapping of Course Outcomes (CO) to Program Specific Outcomes (PSO)

PSO1 PSO2 PSO3

CO1 L H M

CO2 M H M

CO3 M H H

CO4 H H L



SECOND SEMESTER

UPSTREAM PROCESSING






1. Know the advanced techniques of upstream processing.

2. Describe the role of preparation and optimization of media in production of bio-products

3. Comprehend different process controllers and parameters to be evaluated during fermentation.

4. Design and cost analysis of the bioreactor for the plant, animal and microbial boiprocesses

Unit – I 10Hrs

Introduction to upstream process technology: Typical fermentation flowsheets for plant, animal

and microbial processes. Advance culturing techniques for plant and animal cells. Growth kinetics of

microbial, plant and animal cells in Bioreactor. Specialized animal cell culturing techniques for

production of immune based compounds. Different modes of operating the upstream processes in

batch, fed batch and continuous operations. Production of industrial important compounds from

microbes, plant and animal-metabolites, antibodies, vaccines, enzymes, rDNA proteins and

biotransformation molecules.

Unit – II 08Hrs

Media and sterilization: Stoichiometric growth equation for various biomass and simple products –

ethanol. Raw materials selection and media formulations with examples. Media optimization using

statistical methods-Plackett Burman, Central Composite Design and Response Surface and rising

ridge methods. Sterilization: Batch, continuous (Direct and indirect) and filter sterilization for plant

and animal media.

Unit – III 10Hrs

Instrumentation in Bioreactors: Microbial bioreactors-fully automated and sterilizable fermenters.

Instruments required to monitor the various process parameters Measurement of temperature,

pressure, pH, Dissolved Oxygen, foam, product activity, substrate concentration and critical

components and agitation control. Measurement of flow rate of liquid and gases; online estimation of

process parameters by SCADA and case study on SCADA.

Unit – IV 10Hrs

Design of a Bioreactor: Functions, materials of construction, and maintenance of aseptic conditions

of a bioreactor. Types of fermenters: Membrane, animal bioreactors, plant bioreactors, fluidized bed,

Immobilized, packed bed bioreactors and disposable bio reactors. Design of jacketed vessel for batch

and continuous processes. Calculation of thermodynamic aspects for SS416, SAE40 grade and

borosilicate fermenters. Scale up and scale down methods for fermentation, good manufacturing

practices, Estimation of OTR by static and dynamic method. Computer applications in fermentation

technology.



Unit – V 10Hrs

Upstream economics and effluent treatment: Unit operations involved in upstream processing of

various compounds generated from fermentation industry. Cost estimation and development of

balance sheet for various upstream processes. Market value for biotechnological compounds. Effluent

treatment: characteristics of the effluent generated from upstream processes. Physical, chemical and

biological methods of treating the effluents. Case study: Very high value and low volume compound.



CO1. Remember and understand the processes for the production of various bio-products.

CO2. Implement the techniques for Upstream Processing and its parameters optimization

CO3. Analyze the scale up techniques, process economics and effluents management.

CO4. Design of bioreactors involved in upstream processing.

PRACTICALS

1. Cultivation of yeast in fermenter to produce ethanol.

2. Production and extraction of secondary metabolites from callus culture.

3. Isolation of primary cell lines and its maintenance.

4. Scale-up production of wine.

5. Production of antibiotics from bacterial and fungal species, its inhibition studies.

6. Estimation of metabolic respiration rate of yeast.

7. Microbial Pectinase production and purification.

8. Production of proteases from microbes and estimation of its activity.

9. Production and estimation of citric acid from microbes.

10. Production of lactic acid using bacterial culture and its quantitative estimation.

Reference Books:

1. Brian McNeil and Linda Harvey, “Practical Fermentation Technology”, Wiley publishers, 2008.

ISBN: 0470725281

2. Peter F. Stanbury, Allan Whitaker, Stephen J Hall, “Principles of Fermentation Technology”, 2nd

edition Pergamon press., 2003, ISBN: 0080946437.

3. Pauline M. Doran, “Bioprocess engineering principles”, 2d edition, academic press, 2012. ISBN:

978-0-12-220851-5.

4. Michael Butler, “Cell Culture and Upstream Processing”, 1st edition, Taylor & Francis, 2013.

ISBN: 978-0415399692.

http://as.wiley.com/WileyCDA/Section/id-302477.html?query=Brian+McNeil

http://as.wiley.com/WileyCDA/Section/id-302477.html?query=Linda+Harvey












CIE for the practical courses will be based on the performance of the student in the laboratory, every

week. The laboratory records will be evaluated for 40 marks. One test will be conducted for 10

marks. The total marks for CIE (Practical) will be for 50 marks.


SEE for the practical courses will be based on conducting the experiments and proper results for 40

marks and 10 marks for viva-voce. The total marks for SEE (Practical) will be 50 marks.



CO1 H H M M M M - L L

CO2 L H H M H L H - L L

CO3 L L M M L H H - L L

CO4 H H H M L H M - L L


PSO1 PSO2 PSO3

CO1 L M H

CO2 L M H

CO3 L L H

CO4 H M L



IMMUNOTECHNOLOGY

(Elective-2)






1. Understand and utilize various components and mechanism of immune response and reactions

2. Comprehend structure and function of immunoglobulin, antibody and other immunological

adjuvants

3. Apply various techniques for understanding intricacies of immunology

4. Analyse and apply transplantation tools and techniques for immunological reactions.

Unit – I 9 Hrs

Introduction to Immune System, organs, cells and molecules involved in innate and adaptive

immunity.Hematopoiesis and its regulation, role of cytokines, chemokines and leukotrienes,

phagocytosis and microbicidal mechanisms. Immediate hypersensitivity: role of eosinophils, and

mast cells. Genetic bases of immune response – Heterogenecity; Immune modulators.

Unit – II 10 Hrs

Receptors of innate immunity: Toll-like receptors, opsonization, Immunochemistry of Antigens -

Immunogenicity, Antigenicity, haptens, super antigens , Toxins-Toxiods, Hapten carrier system. B

and T cell epitopes, T cell receptors, Activation of T cells, APC-T cell interaction, Differentiation and

activation of B cells, BCR and editing, Major Histocompatibility Complex: genetic organization of

H2 and HLA complexes. Class I and class II MHC molecules, MHC restriction. Antigen processing

and presentation pathways

Unit – III 9 Hrs

Antibody structure and function: Classification of immunoglobulins, immunoglobulin domains,

concept of variability, isotypes, allotypes and idiotypic markers. Antigen-antibody interactions,

Immunoglobulin genes, VJ/VDJ rearrangements and genetic mechanisms responsible for antibody

diversity,Class switching, soluble forms of immunoglobulin, The complement system: classical and

alternative pathways,

Unit – IV 9 Hrs

Hybridoma, monoclonal antibodies, and antibody engineering, Immunological Techniques antibody

generation, detection of molecules using ELISA, RIA, Western blot, immunoprecipitation,

flowcytometry, immunofluorescence microscopy, Insitu localization techniques such as FISH,GISH,

Monoclonal antibodies and their role in cancer.

Unit – V 10 Hrs

Application of immunological principles- Transplantation immunology- immunologic basis of graft

rejection, clinical manifestations, immunosuppressive therapy, Immunostimulants, Vaccines: types,

recombinant vaccines and clinical applications, Tumor immunology and autoimmunity,



CO1: Apprehend the concepts of immunity and immune reactions.

CO2: Analyze the various types of immune responses

CO3: Apply the knowledge of immunology to identify various immunological reactions and

interactions



CO4: Evaluate the significance and applications of various immunological techniques.

Reference Books:

1. Ashim K. Chakravarthy. Immunology and Immunotechnology Oxford University Press. 2006.

2. Kindt, T. R. Goldsby, B. A. Osborne, Kuby Immunology, W. H. Freeman, 6th edition, 2006.

3. Roitt, I. Essential Immunology by Blackwell Scientific Publications, Oxford. 2001

4. Benjamini E. and Leskowitz S. Immunology: A short course, Wiley Liss, NY. 2003.









Mapping of COs with Pos


CO1 - H M L H M - - - - L

CO2 - H M M M H - - - - L

CO3 L M H M M H - - L - H

CO4 L H H M H H - - L H M


PSO 1 PSO 2 PSO 3

CO 1 H M L

CO 2 M M L

CO 3 H H H

CO 4 H H H



NANOBIOTECHNOLOGY

(Elective-2)




Course Learning Objectives:


1. Understand the fundamentals of nanomaterials.

2. Describe methods for their synthesis, characterization and their applications

3. To have awareness about the nanosensors used in diagnostic and therapeutic use.

4. To design a concept for a nanoscale product and their applications in medical field.

Unit – I 9 Hrs

Fundamentals of Nanoscience and Engineering: History, Types of nanomaterials: Fullerenes,

Nanoshells, Quntum dots, Dendrimers, Nanocarriers, Nanofibers, Approaches of Fabrication: Top-

Down and Bottom-up methods of nanofabrication and Nanosynthesis, Biosynthesis of Nanoparticles,

Microbial Nanoparticle production Biomineralization, Magnetosomes. Nanolithography: hard and

soft lithography. Characterization of nanomaterials using spectroscopic (UV-Vis, FTIR and Raman)

and microscopic methods (SEM, TEM, STM and AFM).

Unit – II 9 Hrs

Nanobiomaterials: DNA and Protein based Nano structures. Biomaterial nanocircuitry; Protein

based nanocircuitry; Neurons for network formation. DNA nanostructures for mechanics and

computing and DNA based computation; DNA based nanomechanical devices. Function and

application of DNA based nanostructures. Bionanomaterials in Nature: Lotus leaf as a model self

cleansing system. Gecko foot as a case study for biological generation of adhesive forces. Diatoms as

an example for silicon biomineralization. Mussel inspired nanofiber for tissue engineering.

Biomechanical strength properties of Spider silk.

Unit – III 9 Hrs

Micro & Nano Electromechanical systems and Microfluidics: BioMEMS/BioNEMS: Types of

transducers: mechanical, electrical, electronic, magnetic and chemical transducers. Nano sensors:

Types: Electronic nose and electronic tongue, magnetic nanosensors. mechanical nanosensors:

Cantilever Nanosensors, Microfludics: Laminar flow, Hagen-Peouiselle equation, basic fluid

ideas, Special considerations of flow in small channels, micro mixing, microvalves & micropumps,

Body on a chip and lab on a chip.

Unit – IV 10 Hrs

Nanosensors: Nanofabricated devices to separate and interrogate DNA, Interrogation of immune and

neuronal cell activities through micro- and nanotechnology based tools and devices. Types of

Nanosensors and their applications. Electromagnetic nanosensors: Electronic nose and electronic

tongue, Magnetic nanosensors. Mechanical nanosensors: Cantilever Nanosensors, NanoBiosensors:

NanoBiosensors in modern medicine.

Unit – V 10 Hrs

Medical Nano biotechnology in Diagnostics, therapeutics, drug delivery, Nano Surgery and Tissue

Engineering . Drug Delivery Applications, Bioavailability, Sustained and targeted release. Benefits of

Nano drug delivery system. Use of Microneedles and nanoparticles for targeted and highly

controlled drug delivery. Nano robots in drug delivery and cleaning system. Design of nanoparticles

for oral delivery of peptide drugs. Nanotoxicity assessment: In-vitro laboratory tests on the



interaction of nanoparticles with cells.

Expected Course Outcome:


CO1: Understand and apply the knowledge of nanomaterials and nanobiomaterials to enable health

sector advancements.

CO2. Interpret and apply the techniques of manufacturing and characterization processes.

CO3. Apply the knowledge for various applications in Biomedical field.

CO4. Design devices and systems for various biological applications

Reference Books:

1. Gabor L. H., Dutta J., Tibbals H. F., Rao A., Introduction to Nanosciences, 2008, CRC press,

ISBN- 1420048058

2. Murthy B.S., Shankar P., Raj,B., Rath, B.B. and Murday, J. Textbook of Nanosciences and

Nanotechnology, 2013, Springer, Co-publication with University Press (India) Pvt. Ltd. VCH,

XII. ISBN- 978-3-642-28030-6.

3. Vinod kumar Khanna, Nanosensors: 2013, Physical, Chemical and Biological, CRC press,

ISBN 9781439827123

4. Sandra J. Rosenthal, David W. Wright, NanoBiotechnology Protocols, Springer (1st edition,

2005, ISBN-10 1588292762), 2nd edition, Humana Press, 2013. ISBN- 13 978-158829276










CO1 H M M M L H - - H H H CO2 M H H M H M - - M H M CO3 H H H M H M - - - H H CO4 H H H M H H - - - M M


PSO1 PSO2 PSO3

CO1 H L M

CO2 H L M

CO3 H L M

CO4 H L M



PHARMACEUTICAL BIOTECHNOLOGY

(Elective-3)



Credits : 04 SEE Duration : 100



1. Develop an appreciation and understanding to the pharmaceutical research and development.

2. Determine parameters related to stability and formulation of biopharmacetical products.

3. Acquire the knowledge of drug dose relationship and mechanism of action of drugs

4. Understand and evaluate the different pharmaceutical products and their effects on mankind.

Unit – I 09 Hrs

INTRODUCTION: Configuration and conformation of drug molecules, rational drug design,

various approaches in drug discovery, drug targets and pharmacophores. Physical properties of drugs

- physical form, polymorphism, particle size, shape, density, wetting, dielectric constant, solubility,

dissolution, organoleptic property and their effect on formulation. DRUGS & COSMETIC ACT.

cGMP concepts – Development, Manufacturing Record, Analytical & process Validation,

Equipment & utilitiy Qualification and Calibration, Personnel procedures; Regulatory bodies &

requirements - Indian FDA, WHO GMP,U.S FDA. Schedule-Y. Pre-clinical study requirements,

clinical trial phases, Types of trials and Bioethics, Bioavailability and Bio equivalance studies.

Detection Pharmacopoeia.

Unit – II 08 Hrs

Molecular Modeling in Drug Discovery: Drug discovery process, Lipinski “rule of 5”, Partition

coefficient, Hammet contant, Hansch analysis. Role of Bioinformatics in drug design. Target

identification and validation, lead optimization and validation, Structure and ligand based drug

design, Modeling of target-small molecule interactions, Molecular Simulations, Protein modeling.

Structure Activity Relationship - QSARs and QSPRs, QSAR Methodology, Various Descriptors used

in QSARs

Unit – III 10 Hrs

Drug Pharmacokinetics and Pharmacodynamics: Principles of basic and clinical

pharmacokinetics and pharmacodynamics. Physiology of the absorbing membranes. Mechanisms of

drug absorption - passive and active transport - Fick’s first law - affect of membrane permeability on

oral absorption. Factors affecting bioavailability-Physiological, Adverse drug reactions. Drug

interactions, Bioassay of drugs and biological standardization of immunoagents.

Routes: Oral, Sublingual, Buccal, Parenteral, Topical, Rectal and Inhalation. The pharmacokinetic

implications of various routes of administration- Advantages and Disadvantage of various routes of

administration.

Unit – IV 09.Hrs

Introduction to Vaccinology Classification, active immunization, vaccines technology, perspective

vaccines, means of passive immunization, antibodies in therapy, antibody engineering, monoclonal

antibodies, immunoconjugates - specific drug targeting, immunotoxins.

Immuno-Therapeutics: Overview of drug discovery and development of immuno-drugs. Cytokines

classification, pathways of activation, Therapeutic use of cytokines. Immunomodulators

classification, thymic hormones and synthetic immunostimulators. Compliment pathways

diagnostics, ELISA, Flow cytometry, ELISPOT, immnuno radiology, Basic immunotoxicology -

Principles of testing of immunomodulating and immunotoxicological properties of drugs and



Xenobiotics

Unit – V 12 Hrs

Drug Pharmacology: Chemical transmission and drug action in the CNS. Diuretics, Drugs altering

the pH of urine, excretion of organic molecules. Molecular Cardiology: Congenital Heart Disease,

Inherited Cardiomyopathies, Coronary Atherosclerosis, Derived Nitric Oxide and Control of

Vascular Tone, Hypertension, Cardiac Arrhythmias, Cardiovascular Gene Therapy.

Pulmonology: Asthma, Pulmonary Emphysema. Lung Cancer: The Role of Tumor Suppressor

Genes – Strategies for controlling the diseases.

Drugs acting on GIT: Antacids and anti-ulcer drugs, Laxatives and Anti-diarrheal drugs, Appetite

stimulants and suppressants, Emetics and anti-emetics.

Thyroid hormones and anti-thyroid drugs ACTH and corticosteroids, Androgens and anabolic

steroids, oral contraceptives. Treatment of poisoning, Heavy metals and heavy metal antagonists,

Acute, Sub acute and Chronic toxicity



CO1: Understand the effects and mechanism of action of pharmaceutical products.

CO2. Evaluate the quality of pharmaceutical products and discuss the impact of pharma-products

CO3 Apply knowledge/theory to new situations e.g. the formulation of hypotheses and experimental

design.

CO4. Describe approved biotech products, e.g., indications, advantages, disease impact, & product

limits, & status of pipeline products, e.g., development issues

Reference Books:

1

Pharmaceutical Biotechnology: Fundamentals and Applications by Daan J. A. Crommelin,

Robert D. Sindelar, Bernd Meibohm. Springer Science & Business Media, 2013. ISBN:

1461464862, 9781461464860

2

Pharmaceutical Biotechnology Volume 655 of Advances in Experimental Medicine and Biology

by Carlos A. Guzmán, Giora Z. Feuerstein, Springer Science & Business Media, 2010. ISBN:

1441911324, 9781441911322

3 Pharmaceutical Biotechnology: Drug Discovery and Clinical Applications by Oliver Kayser,

Heribert Warzecha, John Wiley & Sons, 2012, ISBN: 352765125X, 9783527651252

4 Goodman and Gilman's Manual of Pharmacology and Therapeutics by Laurence L. Brunton,

Randa Hilal-Dandan. McGraw Hill Professional, 2013. ISBN: 007176917X, 9780071769174










CO1 M H H H H L L - M H -

CO2 M M M M M - - - L H -

CO3 - H M H - - L - - - H



CO4 H - - - M - - - L M -

Mapping of CO with PSO

PO1 PO2 PO3

CO1 M H H

CO2 L H H

CO3 H L L

CO4 L L L



AGRICULTURAL BIOTECHNOLOGY

(Elective-3)






1 Understand the principles and fundamentals of plant and animal cell cultures and its application.

2. Emphasize on various breeding techniques for crop and livestock improvement.

3. Comprehend the potential applications of genetically modified organisms.

4. Analyze and Apply mechanism for livestock improvement and ethical issues concern.

Unit – I 9 Hrs

Concepts and scope of Agricultural biotechnology. Tissue culture in crop improvement,

Micropropagation. Meristem culture and production of virus-free plants. Haploids in plant breeding;

Anther and microspore culture. Embryo and ovary culture. Somatic hybridization; Protoplast

isolation and fusion, cybrids. Somaclonal variation. Synthetic seeds. Cryopreservation, Production

of secondary metabolites, Elicitation with various biotic and abiotic elicitors.

Unit – II 10 Hrs

Classical and molecular plant breeding: Breeding methods for self and cross pollinated crops.

Conventional methods for crop improvement (Heterosis breeding, Mutation breeding, ploidy

breeding). Self incompatibility and male sterility in crop breeding for crop improvement. Molecular

Breeding: – Molecular tagging of genes/traits. Marker-assisted selection of qualitative and

quantitative traits, Screening and validation; Trait related markers and characterization of genes

involved, Gene pyramiding, Transcript mapping techniques.

Unit – III 10 Hrs

Genetic engineering for crop improvement: Manipulation of Photosynthesis, Nitrogen fixation,

Nutrient uptake efficiency. Molecular mechanisms of biotic stress resistance (Insects, fungi,

bacteria, viruses, weeds) and abiotic stress tolerance (drought and salt) plants. Genetic engineering

for quality improvement of Protein, lipids, carbohydrates, vitamins & mineral nutrients, Concept of

map-based cloning and their application in transgenics.

Unit – IV 10 Hrs

Animal Biotechnology: Fundamentals of animal cell culture. Classical and Molecular breeding in

animals, Marker assisted selection. Animal cloning; Transgenic animals, cloning of animals,

Overview of Embryo Transfer in Farm Animals; Somatic Cell Nuclear Transfer and Other Assisted

Reproductive Technologies. Basic principles for the production of transgenic fish, poultry breeds.

Biosafety: Introduction to Biological Safety cabinets. Biosafety guidelines and Regulatory

frameworks in India, GMOs & LMOs; Roles of Institutional Biosafety Committee, RCGM, GEAC

etc. for GMO applications in agriculture.

Unit – V 9 Hrs

Commercialization of agricultural biotechnology: Green food production, Green house

technology and protected cultivation: Types of Green house, Various component of green house,

Design, criteria and calculation. Green house irrigation system, Pytotrons: Hydroponics and

aeroponics. Organic Farming: Concept of Integrated nutrient management and Integrated pest

management, molecular farming in animals and plants.




After going through this course students will be able to:

CO1:Remember and explain various fundamentals of Agricultural Biotechnology with reference to

breeding techniques and regulatory frameworks

CO2: Apply the knowledge of modern tools to analyze the improvement of agricultural practices

and livestocks

CO3: Evaluate and analyze various parameters of transgenics for crop and livestock improvement

CO4: Create paraphernalia for better usage and production of agri based products

Reference Books:

1. Purohit SS, Agricultural Biotechnology, Agribios India, 2nd ed. 2003, digitalized 2011,

ISBN:81-7754-156-0.

2. Stuart J. Smyth, Peter W.B. Phillips and David Castle, Handbook on Agriculture,

Biotechnology and Development, Edward Elgar Publications, 1st ed,2015 ISBN: 978178347

1355.

3. Adrian Slater, Nigel Scott and Mark Fowler, Plant Biotechnology-The genetic manipulation of

plants, Oxford university press, 2nd ed, 2010, ISBN-13:9780199282616.

4. Maarten J. Chrispeels and David E. Sadava , Plants, Genes, And Crop Biotechnology, Jones

and Bartlett Publishers, 2nd ed. 2003, ISBN-13: 978-0763715861.










PO1 PO

2

PO

3

PO4

PO5 PO6 PO7 PO8 PO9 PO10 PO11

CO1 H H M H H - M L L

CO2 H H M H H - H H L

CO3 M M M H H - M H L

CO4 H H H H L - H H H


PSO1 PSO2 PSO3

CO1 L H L

CO2 H H H

CO3 M H H

CO4 L M L



APPLICATIONS OF RECOMBINANT DNA TECHNOLOGY

(Elective-4)






1. Know the principles and applications of heterologous proteins and other products.

2. Gain the knowledge of recombinant DNA technology for production of important products in

prokaryotes and eukaryotes.

3. Acquire advanced knowledge on molecular farming in plants.

4. Understand the techniques required for animal cell manipulation for production of important

products.

Unit – I 10 Hrs

Therapeutic agents in Microbes: Protein therapeutics: Pharmaceuticals (Human interferons, human

growth hormone, Tumdour Necrosis factor Alpha), Enzymes (Alginate Lyase, Phenylalanine

Ammonia Lyase), HIV inhibitor, Recombinant antibodies (Human monoclonal antibodies, anticancer

antibodies). Nucleic acids as therapeutic agents: Antisense RNA/Oligonucleotides, Ribozymes,

Aptamers, Interfering RNAs.

Unit – II 9 Hrs

Vaccines in microbes: Vaccines: Subunit Vaccines (Herpes Simplex Virus, Foot-and-Mouth

Disease, Cholera, SARs, Staphylococcus aureus, Human Papillomavirus), Peptide vaccines (Foot-

and-Mouth Disease), DNA vaccines, Attenuated Vaccines (Cholera, Salmonella species), Vector

vaccines (Vaccinia virus, Mycobacterium tuberculosis)

Unit – III 9 Hrs

Commercial products in microbes: Restriction endonucleases, Lipase, Amino acids (L-Glutamic

acid), Antibiotics (Undecylprodigiosin, 7-aminodeacetoxycephalospopranic acid), Biopolymers

(Xanthan gum, Melanin, Polyhydroxyalkanoates, Hyaluronic acid), Microbial insecticides (Cry

proteins, Chitinase), Isopropanol, Cellulase, Recombinant spider silk protein.

Unit – IV 10 Hrs

Heterologous proteins in Plants: Amino acids (methionine), Lipids (omega-3 and omega-6 fatty

acids), Vitamins (Vitamin E, β-Carotene), Iron, Starch (α-amylase and glucose isomerase in potato),

Flower pigmentation, Antibodies (IgG antibodies), Edible Vaccines (Cholera),

Unit – V 9 Hrs

Heterologous proteins in Animals: Production of biopharmaceuticals from Milk [Cystic fibrosis

transmembrane regulator (CFTR)], Immunotherapeutic Potential of Antibodies Produced in Chicken

Eggs, Increased muscle mass in Transgenic mice, High levels of omega-6-fatty acids, improving

growth rate (Fish),





CO1: Explain the techniques involved in heterologous protein production in microbes, plant and

animal cells.

CO2: Apply techniques required for recombinant DNA technology for production of compounds in

prokaryotes and eukaryotes.

CO3: Compare and contrast between therapeutic proteins, vaccines between recombinant product

developed from microbes, plants and animals.

CO4: Develop methodology for production of heterologous proteins in microbes, plants and animals.

Reference Books:

1.

Glick BR, Pasternak JJ, and Patten CL, Molecular Biotechnology – Principles and applications

of recombinant DNA, ASM Press, 4th Edition. 2010. ISBN:978-1-55581-498-4.

2.

Brown T.A. Gene Cloning and DNA Analysis – An Introduction, Wiley-Blackwell Science, 6th

Edition, 2010, ISBN: 9781405181730.

3. Clark DP, Biotechnology, Academic Cell, 2 edition, 2015, ISBN-13: 978-0123850157.

4. Wagner R, Hauser H, Animal Cell Biotechnology, De Gruyter,1st edition, 2014, ISBN-13: 978-

3110278866.











CO1 L L L L L - - L M M -

CO2 H M M M M - - L M M -

CO3 H M M M M - - L M M -

CO$ H M M M M - - L M M -

Mapping of Cos with POs

PSO1 PSO2 PSO3

CO1 L L L

CO2 H M M

CO3 L L L

CO4 H H H

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BIOREACTOR ENGINEERING

(Elective-4)






1. Acquire the knowledge of rate law and analyze the rate data of homogeneous reactions

2. Understand the ideal reactor operation, the nonideal flow and residence time distribution

function

3. Understand the models of microbial growth kinetics and their applications

4. Learn about internal and external mass transfer effects and methods of minimizing the

resistances for a heterogeneous system

Unit – I 8 Hrs

Homogeneous reactions: Definition of reaction rate, temperature dependency from Arrhenius

law, integral and differential method of analysis of rate equation, general reaction kinetics for

biological systems: zero, first and second order irreversible reactions (final integral rate equation,

no derivation), numericals.

Unit – II 12Hrs

Ideal reactor operation: Batch reactor operation with Michaelis-Menten kinetics, cell culture in

batch reactor, fed batch operation of mixed reactor, Mixed reactor operation with Michaelis-

Menten kinetics,Continuous cell culture: Optimum dilution rate and critical dilution rate in Ideal

Chemostat, chemostat with immobilized cells, chemostat cascade, Plug flow reactor with

Michaelis-Menten kinetics, comparison between major modes of reactor operation, numericals.

Unit – III 10Hrs

Non Ideal Flow: Interpretation of RTD curve: C, E and F curves, step and impulse input response

for the non ideal reactors. Exit age distribution of fluid in reactors, RTD’s for CSTR and PFR,

calculation of conversion for first order reaction.

Unit – IV 8Hrs

Growth Kinetics of Microorganisms: Transient growth kinetics, Substrate limited growth,

Models with growth inhibitors, Logistic equation, Filamentous cell growth model. Growth

associated and non growth associated product formation kinetics, Leudeking – Piret models,

substrate and product inhibition on cell growth and product formation

Unit – V 10Hrs

Heterogeneous system: Interaction between mass transfer and reaction, concentration gradients

and reaction rates in solid catalysts, steady state shell balance, thiele modulus and effectiveness

factor, concentration profiles for spherical geometry and flat geometries, internal and external

mass transfer effects, minimizing internal and external mass transfer effects

Course Outcomes:

CO 1. Apply the rate law to problems in homogeneous system

CO 2. Apply the design equation of ideal reactors to problems of single reactions

CO 3. Use the microbial growth and inhibition models to solve problems

CO 4. Evaluate the rate equation for catalytic reactions and determine the parameters of rate

expression



REFERENCE BOOKS:

1) Doran P.M., Bioprocess Engineering Principles; Academic Press; 2nded; 2012;

ISBN:978012220851

2) Shuler M. and Kargi F., Bioprocess Engineering; Prentice Hall; 2nd ed; 2002;

ISBN:0130819085

3) Fogler H.S., Elements of Chemical Reaction Engineering; Prentice Hall; 4th ed; 2006;

ISBN:0130473944

4) Panda T., Bioreactors: analysis and design, Tata McGraw Hill education Pvt Ltd, New

Delhi, 1st ed. 2011











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THIRD SEMESTER

Downstream Processing & Product Recovery (Theory and Practice)





Graduates shall be able to

1) Learn about the different methods of cell separations and clarification of broth

2) Understand the basics of purification technology and its applications in Bioprocess

industries

3) Know the new technologies adopted in industries

4) Understand different techniques of viral reduction in biological processes and explore case

studies of current biological products.

Unit – I 08 Hrs

Introduction:

Introduction to downstream processing, biological processes, purification technologies, current

scenario in the bioprocess industries. Process design criteria for low volume high value products

and high volume low value products. Process economics, costing for purification of a bio product.

Unit – II 12Hrs

Filtration and Purification:

Filtration, pre-filtration, depth filtration, mechanism of depth filtration, modules of depth filtration.

Flow rates and pressure variations in depth filtration.

Broth clarification different types of broths and methods for cell removal, rheology of fermentation

broth. Membrane filtration, microfiltration, ultrafiltration, Diafiltration, cross-flow filtration,

transmembrane pressure and Flux calculations with numericals.

Unit – III 12Hrs

Chromatography:

Introduction to chromatography, types of chromatography, protein-A based Affinity

chromatography, mechanism. Membrane chromatography, types of membranes used in bioprocess,

compatibility of membranes, biofouling of membranes, concentration polarization and methods of

control. Membrane chromatography modules and mechanism and scale-up techniques for

purification of bio molecules. Capillary Electrochromatography and simulated moving bed

chromatography.

Unit – IV 08Hrs



Final polishing methods: Drying curve, Batch and continuous dryers, Freeze drying, spray

drying, crystallization.

Virus removal methods: Viral removal and deactivation methods, Viral filtration, uv-radiation,

membrane filtration for virus removal. Methods of operation and scale-up activities for viral

clearance

Unit – V 08Hrs

Case-Studies:

Purification Case studies on monoloclonal antibodies (mAbs), recombinant proteins, bacterial

vaccines, traditional and cell culture based viral vaccines. Downstream processing of albumin and

clotting factors.

PRACTICALS

1) Cell disruption of intracellular biomolecules (ex: yeast cells) and to assay the total protein

or enzyme content

2) Calculation of terminal settling velocity of disrupted yeast cells & to design of thickener

for batch sedimentation (under gravity) using Kynch’s theory

3) Determination of clean water flux (CWF) and to calculate the flux and area of membrane

required for the clarification of known cell broth (ex: yeast cells) in given time

4) Extraction of an antibiotic (ex: ceftriaxone and sulbatum) using different aqueous-organic

solvent systems and determination of distribution coefficient and percentage extraction.

5) To carry out bulk precipitation of protein/enzyme from given suspension (ex; yeast cells)

using ammonium sulfate and find the % cut of ammonium sulfate where the protein is

highest precipitated

6) Determination of the partition coefficient and yield of total protein present in intracellular

or extracellular compounds (such as yeast cells/pigments) using Polyethylene Glycol and

salt system in single or multiple stages.

7) Identification of the unknown pigments such as (leaves of green leafy vegetables) by

comparing its Rf value with Rf value of the standards using thin layer chromatography

8) Determination of the constants of Freundlich equation by adsorbing BSA on silica.

9) Determination of the rate of drying for the given sample in a vacuum tray drier or by



osmotic dehydration (ex: vegetables such as potatoes)

10) Purification of biomolecules (ex: pigments) using gel chromatography or ion exchange

chromatography

Course Outcomes:

CO1: Summarize the current process involved in industrial purification of biological products

CO2: Acquire the knowledge on different filtration and purification techniques.

CO3: Understand the different types of chromatography and viral reduction, removal and its

importance

CO4: Overview on case studies pertaining to bioproducts currently in market

REFERENCE BOOKS:

1) Uwe Gottschalk, Process Scale Purification Of Antibodies, John Wiley & Sons, 2nd

edition, 2017, ISBN: 978-1-119-12691-1

2) Harrison R.G. Todd P. Rudge S.R. and D.P. Petrides, Bioseparations Science and

Engineering, Oxford University Press, 2nd edition, 2015, ISBN: 9780195391817

3) Mokesh Doble, Principles of Downstream Processing in Biological and Chemical

Processes. CRC Press, Taylor & Francis group, 1st edition, 2015, ISBN 9781771881401

4) Nooralabettu Krishna Prasad, Downstream Process Technology: A New Horizon in

Biotechnology, PHI Learning Publications, 1st edition, 2010, New Delhi. ISBN: 978-

81-203-4040-4


CIE will consist of TWO Tests, TWO Quizzes and ONE assignment. The test will be for 30

marks each and the quiz for 10 marks each. The assignment will be for 20 marks. The total marks

for CIE (Theory) will be 100 marks. Scheme of Semester End Examination (SEE):





CIE for the practical courses will be based on the performance of the student in the laboratory,

every week. The laboratory records will be evaluated for 40 marks. One test will be conducted for

10 marks. The total marks for CIE (Practical) will be for 50 marks.


SEE for the practical courses will be based on conducting the experiments and proper results for

40 marks and 10 marks for viva-voce. The total marks for SEE (Practical) will be 50 marks.


https://books.google.co.in/url?id=ZNhCDgAAQBAJ&pg=PA527&q=http://www.wiley.com&linkid=1&usg=AFQjCNEs0B7PUb0bRXCx5QOfOemsEE4qcQ&source=gbs_pub_info_r

https://www.google.co.in/aclk?sa=L&ai=DChcSEwiaoPyR0tvSAhXXJ70KHRDHBlYYABABGgJ0aA&sig=AOD64_25A2n4QmuyObiwWCkqigUcqtokzQ&ctype=5&q=&ved=0ahUKEwjK0faR0tvSAhUgTY8KHd-xAFEQ9A4IWQ&adurl=

https://www.google.co.in/aclk?sa=L&ai=DChcSEwiaoPyR0tvSAhXXJ70KHRDHBlYYABABGgJ0aA&sig=AOD64_25A2n4QmuyObiwWCkqigUcqtokzQ&ctype=5&q=&ved=0ahUKEwjK0faR0tvSAhUgTY8KHd-xAFEQ9A4IWQ&adurl=




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STEM CELL AND TISSUE ENGINEERING






1. Know the types and applications of stem cells.

2. Learn techniques involved in isolation, selection and maintenance of stem cells.

3. Apply the technique for basic growth and differentiation of tissues.

4. Learn methods for the growth of tissues and organs.

Unit – I 9 Hrs

Stem Cells: Concepts and Types of Stem cells: Embryonic, Adult and Induced stem cells.

Embryonic stem cells: Pluripotent, Totipotent and Multipotent cells. Adult stem cells:

Hematopoietic, Neural stem cells, Epidermal and Epithelial stem cell.

Unit – II 9 Hrs

Growth and applications of stem cells: Cell culture methods, Cell isolation, selection, maintenance

of primary and early passage cultures. Clinical potential of stem cells: Organ and tissue regeneration,

cardiovascular treatment, Cell deficiency therapy, treatment of any brain related defects.

Unit – III 9 Hrs

Introduction to Tissue Engineering: History and scope of tissue engineering. The isolation and

handling of human and animal tissue. The major methods of preparing a primary culture. Introduction

to cell adhesion: cell–cell adhesion, cell–matrix adhesion and signalling, cell proliferation, and

differentiation.

Unit – IV 9 Hrs

Basic growth and Differentiation of Tissues: Morphogenesis and tissue engineering-gene

expression, cell determination and differentiation. In vitro control of tissue development: In vitro

culture parameters, growth factors, mechanobiology, tissue development and organ engineering. In

vivo synthesis of Tissue and Organs.

Unit – V 9 Hrs

Tissue engineering for tissue regeneration: using bone marrow mesenchymal stem cells (MSCs)

and adipose derived stem cells (ASCs). Therapeutic strategy for repairing the injured spinal cord

using stem cells. Wound and Disc repair using stem cells. Engineering of tissues: cartilage, bone and

skin. Biomaterials in tissue engineering.



CO1: Explain the importance of stem cell, characteristics and tissue functions for

specialized applications

CO2: Compare various kinds of stem cells and tissues used for regeneration purpose.

CO3: Interpret the techniques for using organ regeneration.

CO4: Apply knowledge for tissue engineered blood vessels, oral bone regeneration and



disc Repair.









devices, Springer, 2014,

Reference Books

1. Song Li, Nicolas L’ Heureux and Jennifer Elisseeff,; Stem cell and Tissue Engineering, world

scientific publications, 2014, ISBN: 13978-981-4317-05-04

2. R Lanza, Langer R and Vacanti J: Principles of Tissue Engineering. Elsevier. 2013. ISBN:

978-0-12-398358-9

3. John P. Fisher, A G Mikos and Joseph D Bronzino; Tissue Engineering. CRC Press. 2007.

ISBN: 0849390265

4. JD Bronzino; Tissue Engineering and Artificial organs, Taylor and Francis, 4th edition 2006,

ISBN: 0849321239.



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FOOD TECHNOLOGY


Hrs/Week L:T:P:S : 4:0:0:0 SEE Marks : 100



Graduates shall be able to:

1.Understand the components of food, principles of food spoilage and techniques for food processing.

2.Illustrate the application of food preservation and food production with improved nutritional

benefits

3.Analyze various modern tools and techniques for food processing and packaging.

4.Evaluate various food adjuvants needed in processing along with regulatory norms.

Unit – I 08 Hrs

Basic constituents, nutritive value and metabolic function of food, Food quality characteristics,

engineering properties of foods, colloidal systems in food, rheological properties-viscosity and

texture, aerodynamics and hydrodynamic characteristics, pneumatic handling Food Microbiology:

Microbial growth and contaminants of food, mechanism of food spoilage. Biochemical changes

caused by microorganism, food borne intoxicants and mycotoxins.

Unit – II 09 Hrs

Principles and objectives of Food Processing Technology, impact of food processing on food

constituents. Principle behind Post-harvest processing technology, processing of coffee, tea, cocoa

and spices. Food preservation by high and ultra-high temperatures, food dehydration: fixed tray,

cabinet drying, tunnel drying. Preservation by freezing. Freeze dehydration and storage, Food

preservation by irradiation treatment. Frozen foods. Food freezing equipment: Air blast, plate and

immersion freezers. Bacteriocins. Fermented food products: yoghurt, pro and prebiotics, Soya foods,

dietary foods, nutritional food supplements, edible films.

Unit – III 09 Hrs

Food additives-definition and classification, acidulants, preservatives, emulsifiers and gums,

Humectants, conditioners and enhancers, Nutritional additives, Sweeteners–Natural and synthetic,

Chelating agents, anti-browning agents, antinutritional factors in foods, nutrition value and nutritional

labeling, functional foods. Food safety levels as per the specifications, determination of acute and

chronic toxicity-NOEL, ADI, PFA regulations in India, Codex, US FDA, FSSAI and GRAS.

Unit – IV 09 Hrs

Introduction to Food packaging, Packaging materials and their physico-chemical characteristics.

quality of packaging materials; metal cans, glass containers, plastic containers and pouches, paper and

paperboard, environmental factors in packaging, Shelf Life estimation, Vacuum Packaging,

packaging materials for newer techniques like radiation processing, microwave and radiowave

processing, high pressure processing, thermal processing as retortable pouches, active packaging,

CO2 and oxygen scavenging, modified atmosphere packaging, cushioning materials, Biodegradable

packaging. Concept of personalized nutrition, Concept of nutraceuticals and nutrigenomics.

Unit – V 09 Hrs

EMERGING PROCESSING TECHNOLOGIES Principles of radiation processing, applications like

disinfestation, pasteurization and sterilization, advantages and limitations; ionizing radiations,

mechanisms of action, High Pressure Processing – principles, mechanism of action, advantages and

disadvantages over conventional processing; Equipment and applications in food industry; Equipment



and applications in food industry. Ohmic heating of foods - principles, mechanism of action,

advantages and disadvantages over conventional processing. Retort processing, UHT, Extrusion - hot

and cold.

Expected Course Outcomes: After going through this course the student will be able to:

CO1: Understand the components of food and principles of food spoilage and techniques for food preservation.

CO2: Know the application of biotechnology for food preservation and food production with improved

nutritional benefits.

CO3: Acquire and apply various food processing techniques to increase the nutritional content and shelf life of

food.

CO4: Comprehend the knowledge of modern tools and applications in food technology with emphasis on

safety norms.

Reference Books:

1. James M, Jay. Food Biotechnology, CBS Publishers, 2nd Edition, 2005.

2. Kalidas Shetty. Food Biotechnology, CRC Press, 1st Edition, 2006.

3. Byong H. Lee Fundamentals of Food Biotechnology 2nd , Kindle Edition,Wiley-Blackwell 2014

4. Smith, P.G. “Introduction to Food Process Engineering”, Springer, 2005.




(Theory) will be 100 marks. Scheme of Semester End Examination (SEE):




Mapping of COs with POs PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11

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HUMAN DISEASES


Hrs/Week : L: T: P: S 4:0:0:0 SEE Marks : 100


Course Learning Objectives:


1. Understand the techniques involved in diagnostics of various types of disease.

2. Differentiate between infection and infestation, pathogenesis, symptoms and diagnosis.

3. Identify certain diseases that are caused due to improper functioning of human organs system.

4. Acquire knowledge on allergies, metabolic and psychological disorders.

Unit – I

10 Hrs

Types of human diseases:

Infectious Diseases: Overview of infectious diseases. Types and Causes of Infectious Diseases.

Bacterial diseases: Pneumonia, Typhoid, Tuberculosis, Diarrhoeal Diseases, Leprosy, Viral diseases:

influenza, dengue, chickenpox, human immunodeficiency virus, Ebola, hepatitis B, Protozoan diseases

Malaria, Leishmaniasis, Fungal diseases: ringworm, athlete's foot, Candidiasis, Tine.

Unit – II 9 Hrs

Non–Infectious Diseases: Diabetes Mellitus – types, Diagnosis and treatment. Cancer- Types and

causes, Cellular genes in cancer – oncogenes, Regulation of cell growth/proliferation, Tumor

Suppressors, Metastasis. Cardiovascular disease-coronary heart disease, cerebrovascular disease,

peripheral arterial disease, rheumatic heart disease congenital heart disease, deep vein thrombosis and

pulmonary embolism.

Unit – III

9 Hrs

Diseases of Immune system: Immuno deficiency- Severe combined immunodeficiency (SCID),

Meningitis, Temporary acquired immune deficiencies. Overactive Immune system- Asthma and

Bronchitis, Eczema, Allergic rhinitis. Autoimmune disease-Type 1 diabetes, Rheumatoid arthritis,

Lupus.

Unit – IV

10 Hrs

Congenital Diseases: Introduction to congenital disease. Causes of congenital disease: Single gene

mutation: alkaptonuria, phenylketonuria, albinism, sickle-cell anemia, hemophilia, color blindness.

Chromosomal aberrations: Down’s syndrome, Klinefelter’s syndrome, Turner’s syndrome.

Environmental factors (cleft palate, harelip).

Unit – V

10 Hrs



Course Outcomes:


CO1: Explain the classification and etiological factors of diseases

CO2: Illustrate techniques of diagnosis for various human diseases.

CO3: Appraise various knowledge areas in the platelets and bleeding disorders.

CO4: Analyze various techniques involved in diagnosis of diseases.

Reference Books:

1. Drew Provan, Andrew Krentz “Oxford Handbook of Clinical and Laboratory Investigation”, Oxford

medical publications, 3rd Edition, 2010, ISBN 0 19-263283-3.

2. Mahajan & Gupta, “Textbook of Preventive and Social Medicine”, 5th Edition, 2013, ISBN: 978-93-

5090-187-8.

3. Harsh Mohan, “Textbook of Pathology”, Jaypee Brothers Medical Publishers., 6th Edition, 2013,

ISBN 978-81-8448-702-2.

4. David A. Warrell, Timothy M. Cox, John D. Firth, Edward J., J R., M.D. Benz, “Oxford Textbook

of Medicine” Oxford Press, 6th Edition, 2014, ISBN: 9812-53-121-1.

Scheme of Continuous Internal Evaluation (CIE) for Theory

CIE will consist of TWO Tests, TWO Quizzes and ONE assignment. The test will be for 30 marks each

and the quiz for 10 marks each. The assignment will be for 20 marks. The total marks for CIE (Theory)

will be 100 marks.

Scheme of Semester End Examination (SEE) for Theory




Mapping of Course Outcomes (CO) to Program Outcomes (PO)


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PROCESS MODELLING & SIMULATION






1) Understand the levels and procedure of optimization of a process

2) Learn desirable and undesirable features in the formulation of an optimization problem

3) Understand and solve the mathematical models using different numerical techniques

4) Get an insight of simulation models and their applications to reactors

Unit – I 08 Hrs

Introduction- Scope and hierarchy of optimization, examples of applications of optimization, the

essential features and general procedure of solving optimization problems, obstacles to

optimization. Classification of models, selecting functions to fit empirical data, the method of least

squares, factorial experimental designs, fitting a model to data subject to constraints.

Unit – II 08 Hrs

Basic concepts of optimization: Continuity of functions, Nonlinear Program (NLP) statement.

Unimodal versus Multimodel functions. Convex and Concave functions and their determination,

Necessary and sufficient conditions for an extremism of an unconstrained function one-

dimensional search quadratic approximation.

Unit – III 12Hrs

Numerical methods, function of one variable, scanning and bracketing procedures, Newton’s,

Quasi-Newton’s and Secant methods of uni-dimensional search, region elimination methods,

polynomial approximation methods, multivariable optimization: Direct methods, random search,

grid search, uni-variate search, simplex method, conjugate search directions, Powell’s method,

indirect methods- first order, gradient method, conjugate method, indirect method- second order:

Newton’s method forcing the Hessain matrix to be positive definite, movement in the search

direction, termination, summary of Newton’s method

Unit – IV 12Hrs



Solution of General form of dynamic models, dimensionless models. General form of linear

systems of equations, nonlinear function. General state-space form. Solving homogeneous, linear

ODEs with distinct and repeated Eigen values. Solving non-homogeneous equation, equation with

time varying parameters. Introduction to systems and modeling - discrete and continuous system -

Limitations of simulation, areas of application - Monte Carlo Simulation. Discrete event

simulation . Random number generation and their techniques - tests for random numbers Random

variable generation

Unit – V 08 Hrs

Analysis of simulation data - Input modeling – verification and validation of simulation

models – output analysis for a single model related to linear regression and generalization

of linear regression technique. Stirred tank heaters: model equations, Isothermal

continuous stirred tank chemical reactors, Biochemical reactors: model equations,

linearization. Case studies.

Course Outcomes:

1) Develop the strategy for selecting functions to fit empirical data

2) Develop the governing equation for systems and solve them using numerical techniques

3) Analyze and solve homogeneous and non-homogeneous equation with time varying

parameters

4) Analyze the simulation data and model the reactor

Reference Books:

1) Edgar, T.F., Himmelblau DM and Leon S Lasdon, Optimization of chemical processes by

Mc-Graw. Hill.2001 ISBN-0-07-0393591

2) Wayne Bequette B., Process Control: Modeling, Design and Simulation, Prentice Hall,

Upper Saddle River, NJ, 2003, ISBN-13: 978-0133536409

3) Jenson, V. G. and Jeffreys, F. V., Mathematical methods in Chemical Engineering,2nd

edition, Academic press, Elsevier, India, 2012, ISBN 13: 9780123844569

4) Jana, Aimya K., Chemical Process Modeling and Computer Simulation, 2nd edition, PHI

Learning Private Limited, New Delhi, India, 2011, ISBN978-81-203-4477-8











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REGULATORY AFFAIRS & BIOBUSINESS

(curriculum should be prepared for 44 hrs)


Hrs/Week : L:T:P:S:: 4:0:0:0 SEE Marks : 100



5. Understand the basic concept of regulatory affairs.

6. Address the safety and quality requirement related to biotechnology and other allied domains.

7. Apply the knowledge for addressing the legal intricacies in product development and

commercialization

8. Design the business model for the reproducible and justiciable bio enterprise

Unit – I 10Hrs

Regulatory framework: USFDA: history, regulatory organization, regulated products: biologics,

drugs, medical devices, combinations & others, overview of regulatory operations for FDA

applications. Regulatory framework in India governing GMOs-Recombinant DNA Advisory

Committee (RDAC), Institutional Biosafety Committee (IBC), Review Committee on Genetic

Manipulation, Genetic Engineering Approval Committee (GEAC), Recombinant DNA Guidelines

(1990), Revised Guidelines for Research in Transgenic Plants (1998), Prevention Food Adulteration

Act (1955), The Food Safety and Standards Bill (2005), Regulation for Import of GM Products Under

Foreign Trade Policy (2006-2007),

Unit – II 10Hrs

Regulatory compliance: Quality System Regulations (QSR), Good Manufacturing Practice (GMP),

Good Laboratory Practices (GLP), Good Clinical Practice (GCP), and FDA inspections and

enforcement, product liability, compliance with non-FDA regulations.

Quality systems: Evolution of quality concepts & practices, quality system planning: objectives,

quality manual and quality plans. Elements of quality system, Unique approaches to quality

management: Risk based approach, ISO, TQM and six sigma, quality systems for research.

Quality control: Development of Product & process specifications, selection of analytical test

methods, reference standards/materials, method validation, control of products, process, raw materials

& manufacturing environment,

Unit – III 10Hrs

Nonclinical studies: nonclinical studies & risk assessment, biopharmaceutical delivery,

pharmacokinetics, pharmacodynamics, applications of pharmacokinetics & pharmacodynamics in

biopharmaceutical development, Safety assessment of biopharmaceuticals: toxicology, Design of a

safety assessment program: in vitro screens, in vivo tests on animal models, test product formulation,

route of delivery, and dosing design, elements of nonclinical study design, quality of nonclinical

studies & GLP and its elements..

Clinical studies: Introduction, organization of clinical research: phases of clinical trials, science of

clinical research, quality in clinical research & GCP, Infrastructure for a clinical trial: individuals,

documents & protocols, collection of clinical data & reporting results (CDM). Clinical trial

operations: Phase 1 Clinical Trials – Frist time in man, Phase 2 Clinical Trials – Proof of Concept,

Phase 3 Clinical Trials – Therapeutic Confirmatory, Phase 4 Clinical Study & REMS, Clinical Trials

for New populations and Global clinical trials. Quality Systems for clinical trials: GCP and its

elements.



Unit – IV 08Hrs

Biomanufacturing: Overview of biomanufacturing requirements, Design in biomanufacture,

technical considerations for biomanufacturing, biomanufacturing life cycle, Quality in

biomanufacturing & GMP, biomanufacturing process and products, analysis & testing (GLP &

NABL), labeling, biomanufacturing facilities, utilities & equipment and their qualification,

Commercialization of Biotech products & Processes: Basic regulatory framework with respect to

Regulated and Non-regulated market practices and procedures. Nature of bioscience enterprises,

Strategies in bio enterprises: startups, spinoffs & makeovers, Factors for promotion of bio

entrepreneurship: Role of science parks, Universities, Finance, Government, public support and

industries,

Unit – V 08Hrs

Bioenterprises: Business plans, Business models, funding of biotech business: Financing

alternatives, Angel funding, Venture Capital funding, funding for biotech in India, Exit strategy,

licensing strategies and valuation. Business laws applied to Biotech industries in India. Organizations

supporting biotech growth, areas of scope, funding agencies in India and biotech policy initiatives.

Bio entrepreneurship in rural areas. Support mechanisms for entrepreneurship: Bio entrepreneurship

efforts in India,

History of pioneer biotech companies: Alembic, Shanta Biotech & Biocon,

COURSE OUTCOMES


5. Comprehend the concept and knowledge of regulatory affairs and bio-business

6. Plan for quality control & quality assurance of a biotech products

7. Analyze the factors influencing the bioenterprises.

8. Develop the business and regulatory approval model for biotechnological domains

REFERENCE BOOKS:

1. Michael J Roy; Biotechnology Operations: Principles and Practices; CRC Press, 2011, ISBN

1439830282, 9781439830284,

2. D. Hyne & John Kapeleris; Innovation and entrepreneurship in biotechnology an International

Perspective: Concepts, theories & cases; Edward Elgar, 2006, ISBN 1843765845,

9781843765844

3. The Business of Biotechnology: From the Bench of the Street: By Richard Dana Ono Published

Butterworth- Heinemann, 2011.

4. Regulatory Framework for GMOs in India (2012) Ministry of Environment and Forest,

Government of India, New Delhi Scheme of Continuous Internal Evaluation (CIE):

CIE will consist of TWO Tests, TWO Quizzes and ONE assignment. The test will be for 30 marks each and

the quiz for 10 marks each. The assignment will be for 20 marks. The total marks for CIE (Theory) will be

100 marks.







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MEDICAL DEVICES


Hrs/Week

L:T:P:S : 4:0:0:0 SEE Marks : 100




1. Understand the principle and applications of using medical devices.

2. Acquire knowledge to use the devices in healthcare.

3. Gain the knowledge of biological phenomenon that helps in the design of engineering

devices.

4. Appreciate the use of engineered devices that mimics biological systems.

Unit – I 10 Hrs

Introduction to Implants: Introduction to medical implants and prosthetics used to mimic natural

body organs or parts. The requirement of implants and various materials used to make implants.

The oral implants: The jaw replacement, artificial single tooth or full denture, and palate

replacement.

Unit – II 9 Hrs

Orthopedic implants: The Implant to correct the problems related to bones, various types of material

used to make artificial bone. The replacement of limb bones and supporting implant. The knee

replacement, types of material to make artificial knee and surgical methods for implant. The partial or

full hip bone replacement.

Unit – III 9 Hrs

Cardiovascular implants: Implants for common problems related to cardiovascular system. Types

of “Stents” for arterial blockages. Types and method for the implant of pace maker.

Replacement/correction of heart valves. The complete artificial heart device in case of total failure of

heart functioning.

Unit – IV 9 Hrs

Auditory implants: The implants used related to auditory system: hearing aids, external ear for the

cosmetic purpose, the middle ear and cochlea implant to correct the sense of hearing.

Optical: Lenses and contact lenses for vision correction. Replacement of opaque lens (cataract) by

artificial lens. The recent advances in retina replacement.

Unit – V 10 Hrs

Non invasive Wearable Medical devices: Explain details of purpose, design and working of signal,

data storage, data integration into information technology and work flow related to following devices:

Overall health record, Stay Fit and Energetic, Continuous Glucose Monitors (CGM) with release of

Insulin, Cardiac Monitoring Device





CO1: Explain the principle of sensing of various bio-devices.

CO2: Apply the bio-devices in the field of healthcare, agriculture, military and environmental sectors.

CO3: evaluate the principles of biological systems as applied to the design of engineering devices

CO4: Assess the potentials and limitations of various biosensors to a given problem.

Reference Books:

1. Tiwari A (Ed), Nordin AN (Co-Ed), Advanced Biomaterials and Biodevices, Wiley,

2014, ISBN: 978-1-118-77363-5

2. Evtugyn G, Biosensors: Essentials, Springer, 2014, ISBN: 978-3-642-40241-8

3. Lantada AD (Ed), Handbook on advanced design and manufacturing technologies for

biomedical devices, Springer, 2014,

4. Jenkins, C.H. Bioinspired Engineering, NY: Momentum press, 2012, ISBN:

97816066502259




(Theory) will be 100 marks. Scheme of Semester End Examination (SEE):






CO1 M M L L M _ _ L L L _

CO2 M M M M H _ _ M L L L

CO3 M M M M H _ _ M L M L

CO5 M M M L M _ _ M L M _


PSO1 PSO2 PSO3

CO1 L L L

CO2 L M M

CO3 L M M

CO4 L M M

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