Raman spectroscopy Raman spectroscopy

Mr/ Abd El mo’ez Ahmed Mohammed “3th group”

“Neutron physics and application in material science and nuclear

application”physics departmentFaculty of ScienceSohag University

Egypt

Faculty of Science

Physics Department

JINR’s group

Supervisor : prof . S.I. Tutunnikov.

Technician : Mr. Kovalev Yura.

Why we use Raman spectroscopy?

Raman spectroscopy is used to

determine the molecular motions,

especially the vibrational one .

Now what is the application?Now what is the application?

We can use Raman spectroscopy in:-We can use Raman spectroscopy in:- Tissue characterization.Tissue characterization.

Tissue Imaging.Tissue Imaging.

Live Cell Imaging.Live Cell Imaging.

Tissue characterizationTissue characterization

Tissue Imaging

Live Cell ImagingAll the sexiness, without the dyes!!All the sexiness, without the dyes!!

h

h(-+) 1)

h 3

2

1

0 S1

3

2

1

0 S0

Ene

rgy

Virtual Level

Rayleigh Raman (inelastic)(elastic) Scattering Scattering

Inelastic Scattering

difference in energy

• Energy transferred from incident light to molecular vibrations

Raman Spectrometer Main view of SOLAR-TII setup

Raman spectrometer’s mechanism.

The laser beam falls on the filter and then it passes to the objective and collides with the Sample.The light will be scattered in all directions and will be reflected to the objective again. After the reflected light reaches the objective it falls down on the spectrometer system and finally to theCCD or PMT detectors.

Principle of Confocal Microscopy

Experimental data of a ceramic material.

400 450 500 550 600 650 700 750

-200

0

200

400

600

Inte

nsity

Wave lenght (nm)

Acknowledgement

Finally I’d like to thank

1- Mr/ Kovalev Yura.

2- Mr/ Valantine.

for their help and efforts.