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From PerkinElmer Life andAnalytical Sciences—Your Total Solutions Provider

Radiomatic Flow Scintillation Analyzers

Flow Cocktails

Radio-HPLC Flow Cells

Radiomatic Flow ScintillationProducts Guide

ProFSA™ and ProFSA Plus for Windows®: The Latest in Radiochromatography SoftwareProFSA and ProFSA Plus (21 CFR Part 11 compatible) offer acomplete approach to method development, direct instrumentcontrol, data evaluation, report generation, and hands-offautomation using the Windows 2000 operating system.

Features include:

• Convenient and easy set up Wizards guide you through themajor instrument functions, including instrument configuration,detector set up, calibration and reporting.

• ProFSA software utilizes quick graphical icon-driven tool barswith single keystroke operation and on-screen prompt lines inthe style of Microsoft Word to simplify the operation andaccess to information and to accelerate the learning curve.

• A single software package can be easily integrated into anyexisting HPLC workstation to provide simultaneous operationand a seamless interface, eliminating the need for an addition-al computer.

• Fully networkable software with multiple trace display andevaluation, quench correction, decay correction, manual andautomatic peak finding, continuous pump control and fraction control.

• A complete evaluation package allows both automatic batch analysis and manual evaluation of measurement and evaluation files.

SDA Processing

A special Statistical Deconvolution Algorithm (SDA) combinedwith patented TR-LSC background reduction provides unprece-dented sensitivity for finding the smallest peaks under low levelcounting conditions. SDA is a statistical image enhancementtechnique applied to radiochromatography. Unlike smoothing, inwhich peaks are typically broadened and sometimes completelylost by being spread into the baseline, SDA provides the ability todiscern if a “blip” on the baseline is real and should be integrat-ed and examined.

Full Connectivity for Increased Productivity

Both ProFSA and ProFSA Plus provide the versatility to connect to third party software and support devices. Full control of gradientHPLC pumps is now possible as well as connection to auto-injectors and fraction collectors. More than one HPLC station can be operated by a single PC. Data from liquid scintillation analyzers can be input either directly or via disk transfer to enable datacomparison and reporting. ProFSA and ProFSA Plus are complete radiochromatography packages and can be used equally effectivelyto quantify the mass channel (UV or RI, etc.) as well as the radioactive channels, since they combine many features from conventionalchromatography systems such as internal and external standardization and peak skimming.

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Evaluation of peaks can be automatic or manual.

Samples are easily added to the measurement que throughthe Add Batch dialogue.

Proven Reliability & PerformanceMakes Us The Leader in FlowScintillation TechnologyThe new PerkinElmer Radiomatic® 610TR and 625TR Flow Scintillation Analyzers continue the tradition of superior detection sen-sitivity, validated performance and proven reliability. Not only do these instruments offer the latest technological innovations withpatented TR-LSC electronic background reduction, luminescence correction and multi-channel analysis, the 610TR and 625TR nowprovide the latest in easy to use chromatography software.

Enhanced Peak Finding AlgorithmProvides Superior Sensitivity

A powerful Peak Finder includes the addition of a Peak Rejectfeature that is independent of the Peak Finder itself whichallows removal of small areas that have been detected as peaksbut are not real. In addition an exclusive feature — LLOD rejec-tion — examines each region and rejects those which are lessthan the Lower Limit of Detection based on a statistical assess-ment of background.

Regulatory and FDA 21 CFR Part 11Compatibility withProFSA Plus ProFSA Plus is designed to fully support GLP and associated regulatory requirements for both laboratory operations and laboratory software design including 21 CFR Part 11 compatibility.

• Configurable Password and user ID protected log in.

• Access Level security controls access to menu itemsand form-level functions to guard against unauthorizedaccess to data.

• Functionality to comply with the FDA 21 CFR Part 11 ruling for electronic signatures and electronic records with system manager configurable control of signature points on key events.

• Complete full GLP audit facilities provide a complete audit trail of analytical and method changes. Audit events are selectable in order to allow the user to define key critical data modification points that require audit. A complete audit trail report with filtering options is available to determine who, when, why, and what.

• An extensive document management system is also included that provides seamless integration of data, tables, graphs and textinto final reports through an automated link to Microsoft Word.

PerkinElmer quality means that Radiomatic flow scintillation analyzers are designed, developed and validatedaccording to ISO 9001 documented procedures. For the European community, PerkinElmer’s radio-HPLC detectorsmeet all European directives as defined by CE standards. A Declaration of Conformity is shipped with everyPerkinElmer radio-HPLC detector.

Ensure data security, integrity and traceability with password protected

instrument log-ins and instrument access security. Rawdata are always protected and never overwritten. Reportsalways include important tracking information includinginstrument serial number, date of acquisition, measurementdata file name and evaluation method.

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Audit trial reports are fully configurable.

A powerful Peak Finder allows the discrimination of smallreal peaks from background noise.

Security levels for each user are configurable by the system administrator with Pro FSA Plus.

Radiomatic® Flow ScintillationAnalyzersSuperior Detection Using TR-LSC® Technologyand Multi-Channel AnalysisPerkinElmer’s Radiomatic flow scintillation analyzers havetaken advantage of the innovations in scintillation countingtechnology to yield an ultra-sensitive radio-HPLC detector.Only on a PerkinElmer radio-HPLC detector can you detectlow-level radiolabeled samples beyond the capabilities of con-ventional radio-HPLC detectors. Quantitate your samplesusing radio-HPLC methods and eliminate the additional stepof fraction collection and liquid scintillation analysis.Performance advantages include:

• Multichannel analyzer (MCA) provides full spectral dataduring chromatographic elution.

• Exclusive Time-Resolved Liquid Scintillation Counting(TR-LSC®) background rejection technology increases signal-to-noise ratio by up to 300%.

• Luminescence detection and correction (LDC) eliminates interferences due to chemiluminescence and reports percentage of luminescence per sampling update.

• Validate Radio-HPLC System Performance with Instrument Self-Normalization and Calibration (SNC) and InstrumentPerformance Assessment (IPA).

IPA measures and stores the eight most critical performance parameters. Detection problems can be identified before they affect your results.

IPA keeps an historical record of critical performance parameters for compliance with GLP.

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High resolution MCA (Multichannel Analyzer) displays a full spectrum enabling you to confirm that there are no surprises—no overlooked impurities and no chemiluminescence interference.

Reduce Waste Disposal Costs with IntegratedFluidics Control

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2. The pressure transducer (1) combined with the flow diverter (2) prevents damage to your flow system. The pressure transducer monitors over-pressure conditions within the plumbing flow path, and the flow diverter directs flow away from the flow cell.

4. Quick change flow cells allow you to change applications in minutes, from beta to gamma to positron detection. PerkinElmer offers a wide selection of flow cell sizes from 35 µL to 5,000 µL and cell types including liquid, packed solid scintillators and external scintillators. Narrowbore and microbore flow cells are available.

3. The waste managerdramatically reduces the volume of hazardouswaste by automatically separating the hot waste from cold waste.

5. The electronic stream splitter allows you to direct anyportion of your valuable sample to a fraction collector. Programmable split ratios maintain peak resolution.

Radiomatic detectors have been designed to increase productivity, minimize the use of bench space and reduce waste disposalcosts. With its integrated, digital fluidics control, a Radiomatic system eliminates external plumbing in front of or on the side of the detector. Flow control functions are digitally controlled and monitored. Simple software instructions setup the detector allowingcontrol of the digital fluidics. This can help you achieve substantial savings in cocktail consumption and waste disposal.

(1)

1. The digital liquid scintillation (LS) pump provides accurate flow rates from 0.1 mL per minute up to 100 mL per minute. Low flow rates are essential for micro-volumeapplications. The LS pump can be turned on and off at any time during the sample run to reduce cocktail consumption and lower waste disposal costs.

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PerkinElmer Provides Integrated Solutions:From a Stand-Alone Detector to PC-Based Radio-HPLC SystemsThe Radiomatic 150TR:

Economizes on Price, Not PerformanceThe Radiomatic 150TR offers unprecedented performance in a low cost system. This radio-HPLC detector is designed to beintegrated into a complete HPLC workstation or operated as astand-alone detector.

• Superior sensitivity with multi-channel analysis and TR-LSCbackground rejection technology.

• Menu-driven software from the front panel keypad providesdetector setup and complete instrument control to minimizethe software learning curve.

• Integrates easily and is compatible with any HPLC workstation.Send raw or processed data to your HPLC workstation fordata evaluation.

An Integral Part of a Chromatography WorkstationThe Radiomatic 150TR can send either raw or processed datavia an analog output signal to your HPLC workstation for fur-ther evaluation. You can also store up to five analytical methods, three quench curve correction files for accurate DPM results, and up to nine sample acquisition runs—features not found on any other stand-alone radio-HPLC detector.

The Radiomatic 610TR:

Setting a New Standard for Radio-HPLC DetectorsThe Radiomatic 610TR provides additional features as standardin a sophisticated radio-HPLC system that operates simultaneous-ly with your HPLC workstation.

• ProFSA or ProFSA Plus (21 CFR Part 11 compatible) softwareprovides the latest in radiochromatography instrument con-trol, evaluation and reporting.

• Unequaled detection sensitivity with multichannel full spectralanalysis and TR-LSC background rejection.

• Front panel display and menu-driven keypad for direct instrument control.

• Self-Normalization and Calibration (SNC), automatic celldetect, a full 1,024 channel MCA including interactive spec-trum analysis for region optimization provide the necessarytools for ease of operation.

Whatever your application—from radiopharmaceutical validation studies to drug metabolism or toxicological studies—theRadiomatic 610TR gives you the flexibility, accuracy, and GLP tools to meet regulatory and standard quality requirements. And,with a standard 24-bit analog input signal with positive and negative signals, 0.1 second sampling time, and a programmable fullscale, you can analyze both your radioactive and mass channel signals simultaneously.

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The Radiomatic 625TR:

The Right System for Any Radio-HPLC ApplicationThe Radiomatic 625TR, the most sophisticated analyzer on the market, provides even more standard features, includingadditional GLP tools and an additional analog input channel.

• Displays radiochromatograms in real time on a high resolution color electroluminescent display.

• Validates system performance with exclusive IPA using standards validated with NIST* activity.

• Displays and analyzes multiple traces simultaneously for profiling studies.

The Radiomatic 625TR delivers all the performance of theRadiomatic 150TR and 610TR plus incorporates a high resolu-tion graphics display for viewing of your chromatograms inreal time. With the Radiomatic 625TR, you get maximum sensi-

tivity, GLP instrument self-calibration and validation. The exclusive IPA system monitors the eight most critical performance parame-ters using standards validated with NIST activity. Control charts track sensitivity, reproducibility, and instrument stability; results arestored on hard disk and a hard copy can be printed on demand.

* NIST (National Institute of Standards and Technology)

Radiomatic System Configuration

150TR 610TR 625TR

MCA S S S

TR-LSC S S S

Two Radiochannels S S S

Luminescence Detection and Correction S S S

Self-Normalization and Calibration O S S

Pressure Sensor/Flow Diverter S S S

Digital LS Pump S S S

Spectrum Analysis S S S

ProFSA/ProFSA Plus N S S

Analytical Audit Trail N S* S*

Instrument Performance Assessment N O S

3-D Analysis N O S

Analog Input O 1 channel** 2 channel**

Analog Output S O O

Programmable Stream Splitter O O O

Waste Manager O O O

Lead Shielding O O O

Instrument Printer O N N

Narrowbore O O O

Microbore N O O

S = StandardO = OptionalN = Not available

*With Pro FSA Plus**24-bit analog input signal

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Versatile Flow CellsChoose the Flow Cell that Meets Your Needs• High sensitivity for liquid cocktail and solid scintillation counting

• Analysis of low and high energy gamma entitles

• Mico volume or ID-live mass spectrometry analysis

• “Quick change” cells are interchangable within the same instrument

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Homogeneous

Homogeneous flow cells with volumes from 100 µL to 5,000 µL provide the lowest backgrounds, highestsignal-to-noise ratio, and highest sensitivity of all flow detection schemes. Liquid scintillation cocktailsuch as PerkinElmer’s Ultima-Flo™ environmentally safe cocktail is mixed with the column eluate in ratiosas low as 1:1 without compromising performance. Our exclusive MCA technology eliminates interferencedue to background, chemiluminescence, and the effects of chemical quenching by spectral stripping.

Microvolume Homogeneous

Microvolume homogeneous flow cells with minimal dead volume fittings and connections are available inboth NarrowBore and MicroBore format and are recommended for FSA/LC/MS applications. NarrowBoreflow cells are available in the range 15 µL to 99 µL while for MicroBore the available range is 3 µL to 14 µL.The combination of microvolume technology with patented TR-LSC background reduction results in bestdetection, thus separating low activity sample components from background. Peak resolution is improved

five-fold which gives enhanced peak separation, elimination of peak tailing and ensures the identification of all sample components.

Heterogeneous

Heterogeneous flow cells with volumes from 50 µL to 300 µL are packed with fine grains of solid scintillatingmaterial within the coiled Teflon® tubing of the flow cell. Heterogeneous detection eliminates consumablecosts, reduces waste, and, because the sample is not mixed with liquid cocktails, allows total samplerecovery. High performance flow cells with volumes of approximately 35 µL, 100 µL, 200 µL and 400 µLoffer heterogeneous detection for applications that require high back pressure and high flow rates. Theseflow cells withstand pressures of up to 2,000 psi. The unique packed cartridge design makes cartridgereplacement easy. This may be necessary if the scintillating material becomes contaminated.

Gamma

Gamma flow cells for low and high energy gamma emitters are ideally suited for the high performanceflow scintillation analysis of samples labeled with low and high energy gamma emitting nuclides. The

Gamma-C flow cell is ideal for low energies below 70 keV such as 125I and 109Cd with counting efficiencybetter than 85% for 125I. The Gamma-B flow cell is suited for mid to high energy nuclides above 70 keVsuch as 99mTc, 131I, and 201Tl. Since the scintillator is in the window, there is no need for liquid cocktail,

thus eliminating cocktail consumption and reducing waste generation.

PET

The PET flow cell is especially suited for the analysis of radiolabeled pharmaceuticals and their metabolitesin Positron Emission Tomography (PET) applications. This flow cell provides PET centers with selective,highly sensitive detection of positron emitters such as 11C, 13N, 15O, and 18F. A unique flow cell designincorporates optically isolated bismuth germanate (BGO) scintillating windows resulting in signal-to-noiseratios unmatched by any other PET flow cell.

Homogeneous Counting—For High Sensitivityand High Efficiency

Homogeneous methods provide the lowest backgrounds, highest sensitivity, highest efficiency andthe best MDA for all isotopes. The homogeneous flow cell consists of Teflon tubing which is coiledbetween two scintillating windows. Teflon tubing is chosen because of its inertness and transparencycharacteristics to maximize light output yielding the highest counting efficiency. The scintillatingwindows form the basis of TR-LSC to achieve unprecedented low backgrounds and the highestsignal-to-noise ratio.

With homogeneous techniques, the column eluate is mixed with the liquid scintillation cocktailand passed through the flow cell. Homogeneous techniques are preferred to heterogeneous tech-niques for low energy isotopes such as 3H and molecules with high molecular weight. Display andanalyze radiolabeled samples using Spectrum Analysis to accurately set energy windows and optimize counting conditions. Quench correction curves are automatically generated to obtain themost accurate DPM results.

Heterogeneous Detection—Reduces Wasteand Recovers SampleFor applications that require total sample recovery, PerkinElmeroffers a wide range of heterogeneous flow cells. Now, you canreduce radioactive waste with cocktail-free counting for a widerange of applications.

Your Choice of Solid Scintillators

Choose from a variety of solid scintillators compatible with manyapplications. Particle sizes vary from 35 µm to 250 µm. Smallparticle sizes mean maximum light output and high countingefficiency. Choose from cerium-activated lithium glass, cerium-doped yttrium silicate, and a new advanced proprietary scintillator,SolarScint™.

High Performance Flow Cells for Normaland Micro-Volume Applications

PerkinElmer’s high performance flow cells are packed with scintillating particles for normal HPLC and micro-volume HPLC.For mass spectrometry applications where peak resolution and minimizing dead volume are important, this specially constructed flow cell provides less than 1 µL dead volume.

In addition, the glass cartridges are replaceable. All packed scintillator flow cells, whether in Teflon tubing or glass cartridges, will eventually become contaminated. Replacing cartridges is more cost effective than replacing the entire flow cell.

PerkinElmer also offers calcium fluoride solid scintillator cells through special order.

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Liquid 3H 14C

Background 5 CPM 8 CPM

Efficiency (unquenched) 52% 93%

Efficiency (quenched) 45% 88%

MDA 120 DPM 60 DPM

PerkinElmer’s proprietary SolarScint™ cocktail provides superbresolution without the memory effects of many other packedscintillators.

Yttrium Silicate

Yttrium silicate is probably the most efficient of the solid scintillatorsbut it is also the one most prone to contamination and it produces luminescence at low pH. Contamination problems are due to the polycrystalline nature of the material. Yttrium silicate is TR-LSCcompatible resulting in low backgrounds and high signal-to-noise ratio.

Lithium Glass

Lithium glass is the most rugged solid scintillator and is extremely resistant to contamination. It can withstand particularly rigorous decontamination procedures. It gives acceptable but lower counting efficiencies for 14C than either yttrium silicate or calcium fluoride, butis rarely used for 3H measurement. However, it is the fastest to darkadapt and the least affected by extremes of pH.

SolarScint

SolarScint’s inertness means compounds with complex matrices will notabsorb to the scintillating particles. Peak broadening, contamination andmemory effects are reduced to a minimum. With small particle sizes of35 to 60 µm, efficiency for 14C is better than 65%. SolarScint is especiallygood for inositol phosphates.

Gamma Flow Cells with the Performance of aDiscrete Gamma Counter

PerkinElmer’s Gamma-C and Gamma-B flow cells are ideally suited forhigh performance flow scintillation analysis without cocktails of sampleslabeled with low and high energy gamma emitters.

The Gamma-C flow cell incorporates europium-doped calcium fluoridescintillating windows to yield better than 85% efficiency for 125I and other“soft” gamma emitters.

The Gamma-B flow cell for mid to high energy gamma emitters such as111In, 22Na, and 99mTc uses special BGO scintillating windows. The highspecific gravity of the Gamma-B flow cell nears and sometimes exceeds theperformance of a discrete gamma counter using a 3-inch NaI detector.

Yttrium Silicate 3H 14C


Efficiency 2.3% 70%

MDA 2,500 DPM 200 DPM

Particle size 35–60 µm 35–60 µm

pH range 3–8 3–8

SolarScint 3H 4C


Efficiency 3% 70%



pH range 2–8.5 2–8.5

Lithium Glass 3H 14C


Efficiency 1.2% 48%



pH range 0–9 0–9

Gamma-C, Gamma-B and PET flow cells

Gamma-C 125I 109Cd


Efficiency 85% 34%

Energy windows 1 0–85 keV 5–60 keV

Gamma-B 99mTc 131I


Efficiency 64% 58%

Energy windows 40–300 keV 220–950 keV

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Ordering Information

Product Cat. No. Size

Flo-Scint II 6013529 2 x 5 L

Flo-Scint III 6013539 2 x 5 L

Flo-Scint IV 60133547 2 x 5 L

Ordering Information

Product Cat. No. Size

Ultima-Flo M 6013579 2 x 5 L

Ultima-Flo M 6013572 20 L

Ultima-Flo AP 6013599 2 x 5 L

Ultima-Flo AP 6013592 20 L

Ultima-Flo AF 6013589 2 x 5 L

Flow Detection CocktailsOptimum Performance and Safety

The Ultima-Flo series can replace the complete range of Flo-Scint and similar cocktails. Ultima-Flococktails outperform the classical cocktails on mixing ratio (up to 1:1). They also provide the user withthe safety features related to the high flash-point solvent system.

Ultima-Flo™ Safer Flow Detection Cocktail LinePerkinElmer’s biodegradable cocktails for use in flow scintillationanalyzers, the Ultima-Flo family, were developed to addressthe concerns of environmental safety. These environmentallysafer cocktails satisfy all of the applications covered by theoriginal, classical solvent-based Flo-Scint™ cocktails. The highloading capacity of the Ultima-Flo cocktails means less cocktailrequired, longer residence times and higher sensitivity. Eachcocktail has low viscosity and non-gelling properties—even athigh sample loads—ensuring trouble free counting and lowerback pressures. Ultima-Flo cocktails resist quench, providinghigher counting efficiencies and more counts per peak.

Ultima-Flo MUltima-Flo M is formulated for multipurpose flow countingapplications. This safer cocktail has a high sample acceptancefor a wide range of dilute HPLC eluents, and methanol and acetonitrile gradients. Ultima-Flo M has low viscosity, uniquerapid mixing properties and is resistant to chemiluminescence.

Ultima-Flo APUltima-Flo AP accepts gradients up to 2.0 M ammonium phosphate with fast and easy mixing. Ultima-Flo AP demonstrates high counting efficiency and quench resistance for a wide variety of sample types.

Ultima-Flo AFUltima-Flo AF is formulated to accept gradients up to 2.0 Mammonium formate at a 1:1 ratio with fast and easy mixing. Thiscocktail is appealing to those who use ammonium formate buffersto elute radiolabeled inositol phosphates from HPLC columns.

Flo-Scint™ Classical Flow Cocktail LineThe classical Flo-Scint cocktails have gained an excellent reputation when gradients with methanol and acetonitrile especially have to be counted. They are resistant to chemi-luminescence and are non-gelling.

Flo-Scint IIFlo-Scint II is a pseudocumene-based flow cocktail that can be used with polar solvents and dilute buffers affording goodsample loading capacity and low viscosity.

Flo-Scint IIIFlo-Scint III is designed to work with methanol and acetonitrilegradients as well as moderately buffered solutions, includingphosphates. Flo-Scint III has good sample loading capacity, lowviscosity and high counting efficiency.

Flo-Scint IVFlo-Scint IV is designed to accept high molarity ammoniumformate and ammonium phosphate gradients (up to 2 M) andalso accepts concentrated salt solutions. Flo-Scint IV is resistantto chemiluminescence, has high counting efficiency, low viscosity and high sample loading capacity.

Dedicated Worldwide Support NetworkPeople and Applications SupportingPerformance Excellence PerkinElmer is recognized worldwide for advanced instrumentation systems and outstanding customer support. We continue to be your partner in life science research providing instruments for scintillation, gamma, and microplate counting; quantitativeimaging; and liquid handling. We are committed to providing the best instrument application support, field service, and telephonesupport. We are proud of our tradition of working with laboratories to accelerate the pace of life science research.

People Putting Technology to Work for You—All Over the World

With over 5,000 instrument installations worldwide, we provide researchers with a total solution of high quality instrumentation andapplication expertise.We have more than 300 highly trained service engineers in more than 60 countries. This makes PerkinElmerthe largest, most well trained radio-HPLC instrument service organization in the world.

Radio-HPLC Bibliography and Application Notes

PerkinElmer’s Radiomatic flow scintillation analyzers are the most cited flow detection systems worldwide. Our current applicationbibliography, available on request, cites over 1,400 articles in open scientific literature describing results of research conductedusing Radiomatic flow scintillation analyzers. Topics include chemical synthesis, drug metabolism, toxicology, other pharmaceuticalstudies, and agricultural/industrial quality control. We continually publish application papers from researchers, and our ownapplication specialists help inform researchers about the latest in radio-HPLC technology.

PerkinElmer products are designed and manufactured under an ISO 9001 certified system. They are designed, built, and tested inconformance with appropriate CSA, FCC, and IEC standards.

For a complete listing of our global offices, visit www.perkinelmer.com/lasoffices

©2003 PerkinElmer, Inc. All rights reserved. PerkinElmer is a registered trademark of PerkinElmer, Inc. All trademarks depicted are the property of their respective holders or owners.PerkinElmer reserves the right to change this document at any time and disclaims liability for editorial, pictorial or typographical errors.

P11019 11/03 Printed in U.S.A.

PerkinElmer Life andAnalytical Sciences710 Bridgeport AvenueShelton, CT 06484-4794 USAPhone: (800) 762-4000 or(+1) 203-925-4602www.perkinelmer.com

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