radioisotope laboratory techniques day 2 environmental health & safety radiation control &...

Radioisotope Laboratory TechniquesDay 2

Environmental Health & SafetyRadiation Control & Radiological Services

UNIVERSITY OF FLORIDARADIATION SAFETY SHORT COURSE

Our Goals

•To provide you with the information to work smarter and safer!

•To fulfill the requirements of the University of Florida’s Radioactive Materials license.

Lab Safety Checklist• Complete Radiation Safety Inspection• Quarterly• Swipe survey• Lists any deficiencies

Lab Safety Checklist1. Caution Signsa. Doorsb. Hoodsc. Hardwared. Work Arease. Refrigerators / Freezersf. Waste Areas

Lab Safety Checklist2. Required Postingsa. Emergency Spill Proceduresb. Notice to Employeesc. Emergency Notificationd. Laboratory Safety Rules

Lab Safety Checklist3. Survey Instrumentsa. Battery Checkb. Calibrated within the last 9 months?c. Proper meter for isotopes in use?d. Is LSC information the same as last

inspection?

Lab Safety Checklist4. Waste Areasa. Properly Shielded?b. Properly Segregated?c. Stored properly?d. Are appropriate catchtrays under

liquids?

Lab Safety Checklist5. Protective Clothinga. Lab coat?b. Appropriate gloves?c. Eye protection?

Lab Safety Checklist6. Lab Surveys and Documentationa. RC-1 Forms current?b. Radioisotope forms maintained

(track RAM from cradle to grave)?

c. Radioisotope transfer forms maintained?

d. Adequate survey frequency and documentation?

e. Spills involving personnel or > 100 mCi?

f. Follow-up surveys, documentation, and decon of contaminated areas > 100mCi?

g. Waste disposal receipts?

UNIVERSITY OF FLORIDA RADIOACTIVE MATERIALS USER STATEMENT OF TRAINING AND EXPERIENCE

(To be completed by ALL personnel who will be working with radioactive materials at the University of Florida)

NAME: DEPARTMENT: PHONE:

CLASSIFICATION (Faculty, Technician, Student, etc.):

RADIOACTIVE MATAERIAL TO BE USED:

PRINCIPAL INVESTIGATOR:

RADIATION SAFETY TRAINING

SUBJECT

WHERE TRAINED

DATES AND DURATION OF TRAINING

PRECEPTOR/ ON THE JOB

FORMAL COURSE

A. Principles and practices of radiation protection

Yes No

Yes No

B. Radioactivity Measurement, standardization, monitoring techniques, and instruments

Yes No

Yes No

C. Mathematics and calculations basic to use and measurement of radioactivity

Yes No

Yes No

D. Biological effects of radiation exposure

Yes No

Yes No

E. Transportation of radioactive materials

Yes No

Yes No

F. Operating and Emergency procedures

Yes No

Yes No

RADIOACTIVE MATERIAL HANDLING EXPERIENCE

RADIONUCLIDE USED

MAXIMUM AMOUNT

WHERE EXPERIENCE WAS GAINED

DATES AND DURATION OF EXPERIENCE

TYPE OF USE

Have radiation exposure records been maintained for you at another institution? Yes No I have read and will abide by the University regulations as set forth in the RADIATION CONTROL GUIDE. Signature: __________________________________________________ Date:

If additional space is needed, use the back of this sheet. Keep a copy and return original to: RADIATION CONTROL DEPARTMENT - 212 Nuclear Sciences Center - Box 118340

RADIONUCLIDE UTILIZATION FORM PRINCIPAL INVESTIGATOR: Dr. No ROOM NO: DEN-252

RADIONUCLIDE: P-32 CHEMICAL/PHYISICAL FORM: liq/aqueous

INITIAL ACTIVITY RECEIVED (Ci or mCi): 5 mCi DATE RECEIVED: 06/04/08

Transferred from PI: Transfer Approval Date:

(USE A SEPARATE FORM FOR EACH SHIPMENT OF EACH RADIONUCLIDE RECEIVED)

Date

Activity Removed Ci-mCi

Activity Remaining Ci-mCi

Use

Final Disposal

Users Initials

06/04/18 0.7 mCi 4.3 mCi Gel shift Liquid waste JT

06/11/08 2.0 mCi 2.3 mCi RNase protection Solid waste DD

06/11/08 0.5 mCi 1.8 mCi Northern Blot Anal. Liquid waste DD

06/12/08 1.8 mCi 0 Disposed remain. activity Solid waste JT

NOTE: When this particular shipment of radioactive material has been completely utilized, decayed or

disposed, maintain this form in laboratory files for review by State and University Radiation Control inspectors.

Lab Safety Checklist6. Lab Surveys and Documentationa. RC-1 Forms current?b. Radioisotope forms

maintained (track RAM from cradle to grave)?













AFTER EACH USE!








University limits for removable contamination:

Beta/Gamma 100 dpm / 100 cm²Alpha 50 dpm / 100 cm²

RADIATION/CONTAMINATION SURVEY

PRINCIPAL INVESTIGATOR: Dr. Who

SURVEYOR: Jack ROOM NO: 13

RADIONUCLIDES USED: P-32 SURVEY NO: 10

SURVEY TYPE: WEEKLY DATE: 05/10/08

Yes No N/A Caution Signs Posted

X

Waste Facilities Posted & Neat

X

Inventory up to Date

X

Work Areas Clean

X

Survey Meter Calibrated

X

Survey Meter Operation Verified with Dedicated Check Source

X

DETAILED LABORATORY LAYOUT

RADIATION READINGS: Survey Meter Model & Serial # Ludlum L-3 SN 5288974

Work Area 0.004 mR/hr Waste Facilities 0.003 mR/hr Storage Areas 0.004 mR/hr CONTAMINATION SURVEY RESULTS Counting Instrument Model & Serial # Beckman LS-8000 SN 8577571152 Detection Efficiency 75% for Iosotope P-32 # Swipe

Identification Net cpm

Net dpm*

Post Decon dpm

1 Bench 74 99

2 Floor 250 333 54

3 Sink 20 26

4 Door 50 67

5 Floor 5822 7762 88

6 Phone 40 53

7 Cabinet 10 13

8 Fuge 44 59

9 Hot plate 13 17

10 Liq waste jug 55 73

11 Desk 10 13

12 Floor 60 80

*Areas of greater than 100 dpm/swipe must be resurveyed and documented. DPM = Gross CPM minus background divided by the efficiency of the counting instrument for the particular radioisotope.

9

8

7

6

5

4

3

2

1

10

12

11

When to document a

survey?

A survey must be documented within seven

days of isotope use(Best to schedule one day

a week)OR

If the lab has isotope storage only (no use) or waste still on hand…a documented survey once a month








Minor Spill

< 100 µCi of activity< 5 mR/hr at one foot

In the Event of a Minor Spill

Notify everyone in the areaPrevent the spread of

contaminationCall for help if needed Decontaminate

• Only trained staff may handle spill• Monitor potentially contaminated staff

first (before leaving the area)• Lab coat and gloves (shoe covers if

necessary)• Start from the outside and work your way

in• Use absorbent paper and detergent as

needed

Survey• Below 100 dpm / 100 cm²

Major Spills

• Personal contamination or injury

• >100 µCi of activity• >5 mR/hour at one foot

In the Event of a Major Spill

Remove individuals from danger

Call 911 if serious injury

Limit the spread of contamination & shield if possible

Call Radiation Control

Close, lock and post the room

Decontaminate under the direction of Radiation Control

Personal Decontamination:

Remove contaminated clothing Use sticky tape Flush with water Soap and warm water Mild abrasives, soft brush and

water Detergents Mild organic acid (citric) Wear gloves

Lab Safety Checklist7. General Lab Safetya. Fume hood calibrated every 12

months?b. Absorbent paper used in

designated work areas (plastic side down)?

c. Food or drinks in work area?d. Proper shielding for isotopes?e. Adequate security?f. Survey of hands and feet after

every use?

Top Priority….. SECURITY!

Must be locked at all times when no one is present

Includes radioactive waste

Challenge everyone that you do not know!

Lab Safety Checklist8. Personnel Monitoringa. Whole body badge?b. Extremity badge (P-32 in

quantities > 1mCi)?c. Exposure reports readily

available?d. Thyroid counts required (I-125 >

1mCi in any 1 month)e. Urine bioassay required for

quantities of H-3 > 25 mCi in any 1 month)

Action Steps

Deficiencies Corrected ASAP

Memos Sent to PI, Department Chair,

Asst. RCO, RCO 10 days to resolve problems

and reply Three memos in 12 months

Radiation Control Committee takes direct action with PI and Department Chair

Questions?

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radioisotope laboratory techniques day 2 environmental health & safety radiation control &...

Documents

lab surveys

radioisotope forms

foot slide

waste areas

month slide

laboratory safety rules

radioisotope transfer

employees c