r-biopharm surefood®/surefast®/ gen-ial® r-t pcr kits · 2019-09-25 · 4plex...
TRANSCRIPT
R-Biopharm SureFood®/SureFast®/GEN-IAL® R-T PCR KitsRT-PCR kits for Microbiology, Animal, Allergen/Plant/Beverages and GMO.
For Pricing and Information Enquire Today!
R-Biopharm Kits for Screening and Species Identification using either the R-Biopharm Rida®Cycler or kits compatible with most other R-T PCR instruments.
For more information, see the product brochures on the next page!
Speak to your local Bio-Strategy Representative for further details or call Customer Service on 0800 34 24 66 or email [email protected]
Bio-Strategy Ltd. T: 0800 34 24 66E: [email protected]://shop.bio-strategy.com
Prices & discounts may be subject to change and only available while stocks last. Not valid
with any other special offer or discounts and cannot be applied in retrospect. All
prices exclude shipping & delivery charges. Promotion code (if stated) must be referenced on your purchase order to receive offer prices.
VALID UNTIL:31 December 2019
Ref. Code: RBIO-PCR
20% discount off
all kits
R-Biopharm AG
SureFast® Salmonella ONESimple and straightforward qPCR for sure food
• Integrated DNA preparation
• Just add lysis buffer
• Heat for 10 minutes
• Use the DNA directly for qPCR
• No centrifugation/automation needed
• Open system for most qPCR thermocyclers
• FAM and VIC/HEX for internal
amplification control
Validated for a broad range of food (poultry meat, meat products, dairy products, vegetables (excluding sprouts),
egg products) and feed.
The validation and certification has been performed in accordance with EN ISO 16140-2:2016 and the MicroVal
and AOAC-RI Rules and Certification Scheme version 8.
The following thermocyclers have been used for the validation:
Roche LightCycler® 480 II, ABI 7500, Bio-Rad CFX96™, BMS MIC, Agilent Aria Mx.
Now certified: MicroVal 2014LR43 AOAC-RI 081803
R-Biopharm AG • An der neuen Bergstraße 17, 64297 Darmstadt, Germany • E-mail: [email protected] • www.r-biopharm.com
R-Biopharm AG
09
/20
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Product Description Tests Art. No.
SureFast® Salmonella ONE Qualitative DNA detection 100 reactions incl. prep F5211
Salmonella Serotype 3plex Qualitative DNA detection of S.typhimurium and S. enteritidis
100 reactions F5159
Salmonella qPCR products
SureFast® Salmonella ONE – simple and straightforward
• Minimal hands on time• Rapid and safe results (IAC)• For all common qPCR thermocyclers
Incubation of
25 g food/feed
in BPW for
16 - 24 hours at
37 °C
1
Inter-
pretation
of results
6
Take 0.5 ml
sample from the
stomacher bag
and add 0.5 ml
lysis buffer from
the kit
2
Mix once and
incubate for
10 minutes
at 95 ºC
3
Transfer 5 µl
DNA samples
(controls) to
20 µl master
mix
4
Add the
controls,
close the
tubes/plate
and start the
qPCR
5
R-Biopharm – dedicated to food safety
• Allergens• GMO• Animal Species• Microbiology
SureFood®/SureFast® real-time PCR kits Product Overview – 03/2019
R-Biopharm – dedicated to food safety
SureFood® – qualitative real-time PCR
Oat S7004
Buckwheat S7005
4plex Wheat/Barley/Rye + IAC S7006
Apricot S7007
SureFood® PREP
Advanced S1053
Extraction control detection kit
SureFast® Animal+Plant Control 3plex F4053
SureFood® ALLERGEN – qualitative and/or quantitative real-time PCR*
Gluten S3606
Soya S3601
Almond S3604
Brazil nut S3617
Cashew S3615
Hazelnut S3602
Macadamia S3616
Peanut S3603
Pecan S3618
Pistachio S3614
Walnut S3607
Sesame S3608
Crustaceans S3612
Fish S3610
Molluscs** S3613
Celery S3605
Lupin S3611
Mustard S3609
SureFood® PREP
Basic S1052
Advanced S1053
Add-On (Use of 2 g sample; Additional to SureFood® PREP Basic)
S1055
Extraction control detection kit
SureFast® Animal+Plant Control 3plex F4053
SureFood® GMO
Plant PLUS S2049
Plant 4plex Corn/Soya/Canola/Cotton S2156
Plant 4plex Corn/Soya/Canola + IAC S2158
SureFood® GMO SCREEN – qualitative real-time PCR
35S/NOS/FMV S2026
4plex 35S/NOS/FMV + IAC S2126
4plex BAR/NPTII/PAT/CTP2:CP4 EPSPS S2127
CaMV S2027
P35S:BAR Rice S2022
4plex BAR/PAT/CryIAb/CTP2:CP4 EPSPS S2128
SureFood® GMO ID – qualitative real-time PCR
MIR162 Corn S2035
MON863 Corn S2037
MS8 Canola S2062
Bt63 Rice S2024
Roundup Ready Soya S2030
RR2Y Soya S2034
A2704-12 Soya S2057
4plex Soya I 1 S2161
4plex Soya II 2 S2162
4plex Canola I 3 S2166
4plex Canola II 4 S2167
4plex Corn I 5 S2170
SureFood® GMO QUANT – quantitative real-time PCR
Bt176 Corn S2015
Bt11 Corn S2016
T25 Corn S2017
MON810 Corn S2019
35S Corn S2020
NK603 Corn S2050
MON863 Corn S2051
MIR162 Corn S2135
GA21 Corn S2054
Roundup Ready Soya S2014
35S Soya S2028
RR2Y Soya S2029
GT73 Canola S2061
TC1507 Corn S2081
MON89034 Corn S2071
SureFood® ALLERGEN/Plant
SureFood® GMO
SureFood® ALLERGEN – qualitative real-time PCR
4plex Soya/Celery/Mustard + IAC S3401
4plex Peanut/Hazelnut/Walnut + IAC S3402
4plex Macadamia/Brazil nut/Pecan + IAC S3403
Laboratory reference material for quantitative real-time PCR
SureFood® QUANTARD Allergen 40 S3301
* With Internal Amplification Control (IAC) using FAM/HEX** Qualitative only
Available soon!
Available soon!Neu
1 MON87708+CV127/DP305423/MON87701/MON877692 RR-Soya/RR-2Yield Soya/A2704-12/A5547-1273 MS8/6GT73/T45 Canola4 MON88302/DPO734906/RF3 Canola5 MON810/TC1507/NK603/MON89034
Available soon!Available soon!
SureFast® – qualitative real-time PCR
Bacillus cereus group PLUS F5126
Emetic Bacillus cereus PLUS F5127
Campylobacter PLUS (C. jejuni, C. lari, C. coli) F5112
Clostridium botulinum Screening PLUS F5110
Clostridium estertheticum PLUS F5160
Clostridium perfringens PLUS F5123
Cronobacter PLUS F5114
Cronobacter sakazakii PLUS F5115
Escherichia coli PLUS F5157
Escherichia coli eae gene PLUS F5104
EHEC/EPEC 4plex (stx1, stx 2, ipaH, E.coli/Shigella) F5128
Histomonas meleagridis ONE F5213
Listeria Screening PLUS F5117
Listeria monocytogenes PLUS F5113
ORT PLUS (Ornithobacterium rhinotracheale) F5164
Salmonella PLUS* F5111
Salmonella ONE** F5211
Salmonella Serotype 3plex F5159
Salmonella Species/Enteritidis/Typhimurium 4plex(R&D version)
F5166
Staphylococcus aureus PLUS F5116
STEC Screening PLUS F5105
STEC 4plex (0157, stx1, stx2, eae) F5165
Vibrio 4 plex (V. cholerae, V. parahaemolyticus, V. vulnificus + IAC)
F5161
Yersinia enterocolitica PLUS F5124
Vibrio Screening PLUS F5118
Vibrio parahaemolyticus PLUS F5120
Foodborne Pathogens 4plex* F5175
MRS-A F7117
SureFast® Microbiology
IAAC = Internal Amplification and Animal Control
FISH ID kits contain 50 reactions and are R&D versions
SureFood® ANIMALSureFood® PREP
Basic S1052
Extraction control detection kit
SureFast® Animal+Plant Control 3plex F4053
SureFood® ANIMAL ID – qualitative real-time PCR
4plex Beef/Sheep/Goat + IAAC S6121
4plex Pork/Chicken/Turkey + IAAC S6123
4plex Beef/Horse/Pork + IAAC S6126
4plex Camel/Horse/Donkey + IAAC S6131
Beef IAAC S6113
Horse IAAC S6118
Horse & Donkey IAAC S6119
Pork SENS PLUS S6017
Pork IAAC S6114
Water Buffalo IAAC S6117
Camel IAAC S6124
Chicken IAAC S6115
Turkey IAAC S6116
Cat & Dog IAAC S6112
Rabbit IAAC S6120
Water Buffalo/Beef + IAAC S6130
Poultry IAAC S6125
SureFood® ANIMAL QUANT – quantitative real-time PCR
Beef S1010
Equus S1016
Pork S1011
Chicken S1014
SureFood® FISH ID – qualitative real-time PCR
Halibut IAAC 3plex (black and white halibut) S6201
Oncorhynchus tshawytscha IAAC (chinook salmon)
S6301
Oncorhynchus mykiss IAAC (rainbow trout) S6302
Oncorhynchus gorbuscha IAAC (pink/humpback salmon)
S6303
Oncorhynchus nerka IAAC (red salmon) S6304
Salmo trutta IAAC (trout) S6305
Salmo salar IAAC (atlantic salmon) S6306
Melanogrammus aeglefinus IAAC (haddock) S6307
Gadus macrocephalus IAAC (pacific cod) S6308
Pollachius virens IAAC (pollock/saithe) S6309
Gadus morhua IAAC (atlantic cod) S6310
Merluccius merluccius (hake) S6311
Merlangius merlangus IAAC (whiting) S6312
Gadus chalcogrammus IAAC (alaska pollock) S6313
Katsuwonus pelamis IAAC S6314
* SureFood® Salmonella PLUS is AOAC-RI (041103) certified.** SureFood® Salmonella ONE is MicroVal (2014LR43) and AOAC-RI (081803) certified.
SureFast® PREP
Bacteria F1021
Aqua F1023
DNA / RNA Virus F1051
Speed Prep F1054
PREP Salmonella F1007
SureFast® real-time Reverse Transcriptase PCR (qualitative)
Norovirus PLUS F7001
Norovirus/Hepatitis A 3plex F7124
Hepatitis A PLUS F7125
Hepatitis E PLUS F7142
Influenza H5/H7/H9 4plex F7139
Influenza A PLUS F7103
SureFast® water analysis
Legionella pneumophila PLUS F5501
Legionella Screen PLUS F5502
Legionella 3plex F5505
Pseudomonas aeruginosa PLUS F5503
Parasitic Water Panel 4plex F5506
Enterobacteriaceae Screening PLUS F5507
Neu
R-Biopharm AG • An der neuen Bergstraße 17, 64297 Darmstadt, Germany • E-mail: [email protected] • www.r-biopharm.com
R-Biopharm – dedicated to food safetyR-Biopharm – dedicated to food safety
SureFood® kits are distributed worldwide by R-Biopharm AG. The test systems are produced by CONGEN Biotechnologie GmbH located in Berlin, Germany.
The SureFood®/SureFast® product range integrates effective sample preparation with high quality real-time PCR kits.
SureFood®/SureFast® PREP nucleic acid extraction kits are characterized by:
• Easy to use spin filter system• Matrix optimized methods• Effective extraction from highly processed food samples• Only standard laboratory equipment required
SureFood®/SureFast® real-time PCR kits are developed based on long year experience of food analysis on DNA/RNA basis. They cover all relevant applications in fields of allergen, GMO and
Benefits
pathogen detection as well as animal speciation with following main specifications:
• High sensitivity and specificity• High reliability• Robustness• Direct application support by producer
The SureFood®/SureFast® kits are available for common real-time PCR devices. For kits with internal amplification control this is realized by the PLUS or IAAC system suitable for common qPCR cyclers:
PLUS 563 - 640 nm
• PLUS kits require a 2-channel device (FAM/HEX) • 3plex kits require a 3-channel device (FAM/HEX/Cy5) • 4plex kits require a 4-channel device (FAM/HEX/ROX/Cy5)
Color compensation kits are mandatory for LightCycler® 2.0 and LC480.
Accessories
AccessoriesSureCC Color Compensation Kit I
Color Compensation for multiplex application of SureFood® kits on LC480
For 3 calibration runs F4009
SureCC Color Compensation Kit II
Color Compensation for multiplex application of SureFood® kits on LC2.0
For 3 calibration runs F4010
Product Description No. of Tests / Amount Code No.
The SureFast® ONE kits contains the relevant lysis buffer for fast DNA preparation. An additional DNA preparation kit is not needed.
03/2
019
Animal and Plant Control + ICD (Art. No. F4053)Nearly all food and feed comprises of plant or animal origin. To ensure a successful DNA extraction result under given circumstances (matrix, PREP kit and laboratory); this extraction control may be used. Adding an internal control DNA (ICD) to the sample preparation helps to control the extraction procedure. Moreover, a differentiation between the DNA of vertebrates and plants is possible. In case of a positive animal signal, the food cannot be declared as vegetarian.
R-Biopharm • An der neuen Bergstraße 17, 64297 Darmstadt, Germany • E-mail: [email protected] • www.r-biopharm.com
R-Biopharm – dedicated to food safety
Molecularbiological wine analysis
with real-time PCR Yeasts and Bacteria
• Precoated PCR tubes• Integrated yeast lysis• Reduced pipetting steps
New
Desirable or undesirable bacteria
For a controlled malolactic fermentation,
Oenococcus oeni is added to the wine. It effects
the transformation of malic acid into milder lactic
acid. However, depending on the desired type of
wine, the presence of Oenococcus oeni may also be
undesirable.Undesirable bacteria include these ones
forming acetic acid.
R-Biopharm – dedicated to food safety
Yeasts analysis for wine becoming increasingly important
Usually, cellar technically controlled fermentation
runs with the addition of selected yeasts mostly
from Saccharomyces strains and possibly the use of
Oenococcus oeni to control the optional malolactic
fermentation.
A spontaneous fermentation with wild yeasts
present in the environment can be difficult to
control. But even with a controlled fermentation,
wild yeasts and specialized bacteria can cause a
sensory change and adverse effects on the wine.
The analyses of the yeast Brettanomyces (Dekkera
bruxellensis) is becoming increasingly interesting.
It is the most important wild yeast in wine, forming
phenolic metabolic products 4-EP (4-ethylphenol)
and 4-EG (4-Etlygujacol). Mainly, it causes blemished
smells described as leather, sweat and/or horse.
Brettanomyces, however, can also be specifically
used in low concentrations to achieve a stronger
sensory effect.
An early quantitative identification of this yeast is of
great interest. The traditional microbiology needs
an incubation time of about 10 days and moreover,
the differentiation of Dekkera bruxellensis requires a
lot of experience to avoid any misinterpretations.
With the help of a DNA standard (DBST 0100)
of 200,000 cfu and derived DNA, a simple
quantification in FAM channel can be done even
with dark red wine. In ROX or HEX channel, an
internal amplification control is performed for each
sample. Optionally, other yeasts are of interest such
as Pichia strains.
Scientific methods are now applied in modern wine production. Wine-making starts
with grapes and the production of grape juice, followed by maceration,
fermentation, aging, clarification and stabilization before ending with the bottled
wine. Many parameters must be or can be tested during wine production in order to
allow decision-making at different stages.
Mikroskopic picture of Dekkera bruxellensis
(LVWO Weinsberg)
The new multiplex kit GEN-IAL QuickGEN First-Wine
PCR Kit (Art. No. QTPWS0096 high/low/white)
detects
• Lactobaccillus, Pediococcus, Oenococcus oeni
(FAM)
• Acetic acid forming bacteria (ROX)
• Yeasts (Cy5)
• Internal Amplification Control (HEX)
and enables a fast overview of the microbiological
status of the wine.
GEN-IAL® First Yeast Dekkera bruxellensis Art. No. TPYDB 0050 FR
Precoated PCR strips – a unique solution for convenient handling
* 4plex assays for MyGo Pro requires a specific kit (QTPWS0096lowMG).
The QuickGEN kits contain 8-well strips which
are precoated with the reagents for up to 4
different parameters per tube. 8-well strips
detecting one to four parameters can be used
for 8 samples/reactions. As extension of this
multiplexing each tube of a 8-well strip may
contain different parameters – this allows a
multiplex panel for up to 4 x 8 parameters for
one sample in a 8-well strip.
Templates are available for MyGo Pro and
qTOWER3. These prepared templates contain
the settings for dedicated kits and allow a direct
start of the real-time PCR without the need to
program the settings:
Open the template of dedicated kit/
parameter
Add the sample names
Start run
1
2
3
Different combinations of screening and identification possibilities are available,
thus allowing a cost effective and customized routine analysis.
High Low White
Agilent MX3005P IT-IS MyGo Pro* Roche Lightcycler® 480 II
Applied Biosystems® ABI 7500 or higher
Agilent AriaMX BioRad CFX96™
ThermoFisher QuantStudio®5 or higher
BioRad CFX96™ Analytik Jena qTOWER3
Product Description No. of Tests/Amount Art. No.
GEN-IAL® Products
DNA preparation
Simplex® Easy Wine kit DNA preparation of wine samples 100 reactions SEW0100
QuickGEN Sample preparation centrifugation
DNA preparation of beverage samples 100 reactions CSE 0100*
Qualitative real-time PCR
GEN-IAL® QuickGEN* First-Wine PCR Kit
DNA screening and differentiation of wine spoilage bacteria and yeasts: Lactobacillus; Pediococcus; Oenococcus oeni; acetic acid bacteria; yeast
96 reactions QTPWS0096 highQTPWS0096 lowQTPWS0096 low MGQTPWS0096 white
GEN-IAL® QuickGEN* First-Wine PCR Kit
DNA Screening of wine spoilage bacteria: Lactobacillus; Pediococcus; Oenococcus oeni; acetic acid bacteria
96 reactions QTPWSOH0096 highQTPWSOH0096 lowQTPWSOH0096 white
GEN-IAL® QuickGEN* First-Oenococcus Oeni
Specific DNA detection of Oenococcus oeni 48 reactions QTPOE0048 highQTPOE0048 lowQTPOE0048 white
GEN-IAL® First-Wine Screening Biogene Amine
Specific DNA detection of bacteria forming biogenic amines
50 reactions BAM0050
GEN-IAL® QuickGEN* Acetic acid bacteria
Specific DNA detection of acetic acid bacteria 48 reactions QTPA0048 low QTPA0048 white
GEN-IAL®QuickGEN*First-Yeast PCR Kit Dekkera spp.
Specific DNA detection of Dekkera spp. 48 reactions QTPYD0048 high QTPYD0048 low QTPYD0048 white
GEN-IAL® Dekkera bruxellensis Specific DNA detection of Dekkera bruxellensis 48 reactions QTPYDB0048 high QTPYDB0048 low QTPYDB0048 white
GEN-IAL® Zygosaccharomyces bailii
Specific DNA detection of Zygosaccharomyces bailii 48 reactions QTPYZB0048 high QTPYZB0048 low QTPYZB0048 white
GEN-IAL® QuickGEN*First-Yeast PCR Kit Wild Yeast
DNA screening and differentiation of wild yeast 96 reactions QTPWY0096 lowQTPWY0096 white
GEN-IAL® QuickGEN*First-Yeast differentiation PCR Kit
DNA screening and differentiation of 12 yeasts 96 reactions QYDIF0096 high QYDIF0096 low QYDIF0096 white
GEN-IAL® Dekkera anomala Specific DNA detection of Dekkera anomala 50 reactions TPYDA 0050
GEN-IAL® Pichia anomala Specific DNA detection of Pichia anomala (Wickerhamomyces anomalus)
50 reactions TPYPA 0050
Accessories Multiplex Screening
GEN-IAL® Dekkera bruxellensis Standards
DNA standards for Dekkera bruxellensis quantification 200.000 cfu DBST 0100
Color Compensation Kit LightCycler® 480
Color compensation kit for multiplex assays 5 reactions PP1TCC 0005
Color Compensation Kit LightCycler® 2.0
Color compensation kit for multiplex assays 5 reactions CCFH 0005
Washing solution Washing solution for SEW 0100 43 ml WS 0100
* Compatible only with the QuickGEN line.** Lyticase is already lyophilized in PCR tubes already submitted.
R-Biopharm – dedicated to food safety
R-Biopharm • An der neuen Bergstraße 17, 64297 Darmstadt, Germany • E-mail: [email protected] • www.r-biopharm.com
02
/20
19
Real-time PCR
• Quick results (approx. 2 hours)
• Highly specific
• Easy handling: Lyticase precoated PCR tubes
• QuickGEN reduces the pipetting steps:
less effort and increased safety
• Even performable in small wine laboratories
R-Biopharm AG
R-Biopharm – dedicated to food safety.
RIDA®CYCLERTemplate and assay overview
Real-time PCR setup
• SureFood® ALLERGEN• SureFood® ANIMAL• SureFood® GMO• SureFast® MICROBIOLOGY
R-Biopharm AG
The tables below show the assays which can be run in parallel on the RIDA®CYCLER. Pick one template and the corresponding assays.
SureFood®ALLERGEN
Art. No. Assay nameTemplate Profile SureFood® ALLERGENS7004 SureFood® OatS7005 SureFood® BuckwheatS3601 SureFood® ALLERGEN SoyaS3602 SureFood® ALLERGEN HazelnutS3603 SureFood® ALLERGEN PeanutS3604 SureFood® ALLERGEN AlmondS3605 SureFood® ALLERGEN CeleryS3606 SureFood® ALLERGEN GlutenS3607 SureFood® ALLERGEN WalnutS3608 SureFood® ALLERGEN SesameS3609 SureFood® ALLERGEN MustardS3610 SureFood® ALLERGEN FishS3611 SureFood® ALLERGEN LupinS3612 SureFood® ALLERGEN CrustaceansS3613 SureFood® ALLERGEN Molluscs (qualitative only)S3614 SureFood® ALLERGEN PistachioS3615 SureFood® ALLERGEN CashewS3616 SureFood® ALLERGEN MacadamiaS3617 SureFood® ALLERGEN Brazil nutS3618 SureFood® ALLERGEN Pecan
Profile SureFood® ALLERGEN 4plex/Profile SureFast® Animal+Plant Control
F4053 SureFast® Animal + Plant Control + ICDS3401 SureFood® ALLERGEN 4plex Peanut/Hazelnut/Walnut + IACS3402 SureFood® ALLERGEN 4plex Soya/Celery/Mustard + IACS3403 SureFood® ALLERGEN 4plex Macadamia/Brazil nut/Pecan + IAC
SureFood® ANIMAL
Template Profile SureFood® GMOS6121 SureFood® ANIMAL ID 4plex Beef/Sheep/Goat + IAACS6123 SureFood® ANIMAL ID 4plex Pork/Chicken/Turkey + IAACS6126 SureFood® ANIMAL ID 4plex Beef/Horse/Pork + IAACS6130 SureFood® ANIMAL ID 3plex Water Buffalo/Beef + IAACS6113 SureFood® ANIMAL ID Beef IAACS6118 SureFood® ANIMAL ID Horse IAACS6119 SureFood® ANIMAL ID Horse & Donkey IAACS6017 SureFood® ANIMAL ID Pork SENS PLUSS6124 SureFood® ANIMAL ID Camel IAACS6117 SureFood® ANIMAL ID Waterbuffalo IAACS6115 SureFood® ANIMAL ID Chicken IAACS6116 SureFood® ANIMAL ID Turkey IAACS6112 SureFood® ANIMAL ID Cat & Dog IAACS6120 SureFood® ANIMAL ID Rabbit IAAC
Art. No. Assay name
Template Profile SureFood® ANIMAL QUANT Beef S1010/ Profile SureFood® ANIMAL QUANT Pork S1011
S1010 SureFood® ANIMAL QUANT BeefS1011 SureFood® ANIMAL QUANT Pork
Profile SureFood® ANIMAL QUANT Chicken S1014/ Profile SureFood® ANIMAL QUANT Equus S1016
S1014 SureFood® ANIMAL QUANT ChickenS1016 SureFood® ANIMAL QUANT Equus
SureFood® GMO
Template Profile SureFood® GMOS2049 SureFood® GMO Plant PLUSS2056 SureFood® GMO PLANT PlantS2026 SureFood® GMO SCREEN 35S + NOS + FMVS2126 SureFood® GMO SCREEN 4plex 35S/NOS/FMV + IACS2127 SureFood® GMO SCREEN 4plex BAR/NPTII/PAT/CTP2:CP4 EPSPSS2027 SureFood® GMO SCREEN CaMVS2022 SureFood® GMO SCREEN P35S:BAR RiceS2063 SureFood® GMO SCREEN CrylAbS2024 SureFood® GMO ID Bt63 RiceS2035 SureFood® GMO ID MIR162 CornS2037 SureFood® GMO ID MON863 CornS2062 SureFood® GMO ID MS8 CanolaS2024 SureFood® GMO ID Bt63 RiceS2030 SureFood® GMO ID Roundup Ready SoyaS2034 SureFood® GMO ID RR2Y SoyaS2057 Su reFood® GMO ID A2704-12 SoyaS2161 SureFood® GMO ID 4plex Soya IS2166 SureFood® GMO ID 4plex Canola IIS2167 SureFood® GMO ID 4plex Canola IIS2015 SureFood® GMO QUANT Bt176 CornS2016 SureFood® GMO QUANT Bt11 CornS2017 SureFood® GMO QUANT T25 CornS2019 SureFood® GMO QUANT MON810 CornS2020 SureFood® GMO QUANT 35S CornS2050 SureFood® GMO QUANT NK603 CornS2051 SureFood® GMO QUANT MON863 CornS2135 SureFood® GMO QUANT MIR162 CornS2054 SureFood® GMO QUANT GA21 CornS2014 SureFood® GMO QUANT Roundup Ready SoyaS2028 SureFood® GMO QUANT 35S SoyaS2029 SureFood® GMO QUANT RR2Y Soya
Profile SureFood® GMO Plant 4plexS2156 SureFood® GMO Plant 4plex Corn/Soya/Canola/CottonS2158 SureFood® GMO Plant 4plex Corn/Soya/Canola + IAC
SureFood® ANIMAL
This video shows you how to upload a template in the RIDA®CYCLER software
R-Biopharm AG
R-Biopharm AG • An der neuen Bergstraße 17, 64297 Darmstadt, Germany • E-mail: [email protected] • www.r-biopharm.com
08/2
018
SureFast® MICROBIOLOGY
Art. No. Assay name
Template Profile SureFast® Salmonella PLUSF5111 SureFast® Salmonella PLUS
Profile SureFast® MICROBIOLOGYF5104 SureFast® Escherichia coli eae gene PLUSF5105 SureFast® STEC Screening PLUSF5110 SureFast® Clostridium botulinum Screening PLUSF5126 SureFast® Bacillus cereus group PLUSF5127 SureFast® Emetic Bacillus cereus PLUSF5112 SureFast® Campylobacter PLUS (C. jejuni, C. lari, C. coli)F5113 SureFast® Listeria monocytogenes PLUSF5114 SureFast® Cronobacter PLUSF5116 SureFast® Staphylococcus aureus PLUSF5117 SureFast® Listeria Screening PLUSF5123 SureFast® Clostridium perfringens PLUSF5124 SureFast® Yersinia enterocolitica PLUSF5128 SureFast® EHEC/EPEC 4plex (stx1, stx 2, ipaH, E.coli/Shigella)F5157 SureFast® Escherichia coli PLUSF5159 SureFast® Salmonella Serotype 3plexF5160 SureFast® Clostridium estertheticum PLUSF5161 SureFast® Vibrio 4 plexF5164 SureFast® ORT PLUS (Ornithobacterium rhinotracheale)F5165 SureFast® STEC 4plex (0157, stx1, stx2, eae)F5211 SureFast® Salmonella ONEF5213 SureFast® Histomonas meleagridis ONEF5501 SureFast® Legionella pneumophila PLUSF5502 SureFast® Legionella Screen PLUSF5503 SureFast® Pseudomonas aeruginosa PLUSF5505 SureFast® Legionella 3plexF5506 SureFast® Parasitic Water Panel 4plexF5507 SureFast® Enterobacteriaceae Screening PLUS
Profile SureFast® MICROBIOLOGY RNAF7001 SureFast® Norovirus PLUSF7124 SureFast® Norovirus/Hepatitis A 3plexF7125 SureFast® Hepatitis A PLUS
Profile SureFast® MICROBIOLOGY RNA60F7103 SureFast® Influenza A PLUSF7139 SureFast® Influenza H5/H7/H9 4plexF7142 SureFast® Hepatitis E PLUS
R-Biopharm AG
R-Biopharm – dedicated to food safety.
RIDA®CYCLERThe multiplex real-time PCR instrument
User-friendly magnetic induction real-time PCR system for SureFood®and SureFast® PCR kits• Easy to use • Fast• Compact
R-Biopharm AG
Features
Compact Portable and compact qPCR instrument with Bluetooth technology
Fast Fast qPCR results through rapid heating and cooling using a patented magnetic induction technology and fan forced air for cooling
Easy High speed centrifugation ensures samples spin down at the start of a run – no extra centrifuge needed
Pre-loaded oil overlay inside each tube prevents evaporation and condensation
User-friendly software set-up with pre-loaded thermoprofiles
Operate up to 10 instruments from one workstation
Dimensions 130 x 150 x 150 mm (H x W x L)
Weight 2.1 kg
Optical
Detectors Photodiode per channel
Excitation Sources High power LED for each channel
4 Channels Green Ex 465nm Em 510nm
Yellow Ex 540nm Em 570nm
Orange Ex 585nm Em 618nm
Red Ex 635nm Em 675nm
Tube format 0.1mL strips of four tubes and matching caps 48 samples per instrument
Thermal performance Temperature accuracy: ± 0.25 °CTemperature uniformity: ± 0.05 °C
Ramp rates Heating: 4 °C/s (fast mode)Cooling: 3 °C/s (fast mode)
Computer minimum requirements
• Windows® 7, 32-bit (English version) Operating System• .NET Framework 4.5 or higher • Intel i5 processor, 2.4 GHz• 4 GB of RAM• 1 GB free hard drive capacity
• Pointer device • USB Drive• Adobe® Reader® must be installed to be able to view reports in PDF format• Bluetooth®, if this is the preferred connection
Calibration
No calibration required – RIDA®CYCLER is a non-contact system. The rotor is not in contact with anything physical just the induction field.
Specifications
RIDA®CYCLER Software
4
21
3
Absolute Quantification: Quantify the unknown concentration of a sample using a standard curve generated with samples of a known quantity
Results
Analysis options:
Cycling: Determination of the quantification cycle (Cq)/ cycle treshold (ct) and reaction efficiency of each sample
Start RunEnter Samples Names
Select the Assay(s)Select the template
Report options: Configure the report as needed and export it e.g. as PDF
User-friendly software set up with all SureFast® and SureFood® assays pre-loaded. Start the qPCR run in only 4 steps:
qPCR Run
R-Biopharm AG
R-Biopharm AG • An der neuen Bergstraße 17, 64297 Darmstadt, Germany • E-mail: [email protected] • www.r-biopharm.com
Software Software Art. No.
RIDA®CYCLER 4 channel qPCR instrument, incl. 1 box of tubes ZRCYCLER
RIDA®CYCLER-MIC-Tubes Box with 960 tubes and caps ZRC-MIC-TUBES
03/2
018
Product range:
• Allergens• GMO• Animal species• Microbiology
SureFast® and SureFood® real-time PCR Kits
Ordering information
All kits are validated on the RIDA®CYCLER
SureFood®/SureFast® real-time PCR kits Product Overview
Size – qPCR instrument with a small footprintModular – Connect multiple instruments wireless to one PCRConvenient – Pre-loaded assays – no more programmingReliable results – Validated for SureFast® and SureFood® real-time PCR Kits
• Simple
• Fast
• Accurate results
Benefits
For further information please visit: www.r-biopharm.com