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Quit Diffusion and Osmosis Start Slide 2 Quit TABLE OF CONTENTS Diffusion Osmosis Living Cell Osmosis Diffusion and Osmosis Slide 3 Quit DIFFUSION Dialysis Equipment Equipment Set Up Results Diffusion and Osmosis Slide 4 Quit Diffusion (Dialysis Equipment) pipet closures glass dish glucose test strips glucose test strips dialysis tubing dialysis tubing Diffusion and Osmosis Slide 5 Quit StarchGlucoseWater Initially, the dialysis tubing is filled with a super saturated glucose + starch solution continue animation dialysis tubing Diffusion and Osmosis Slide 6 Quit StarchGlucoseWater The filled dialysis tubing is placed in a dish with a solution of iodine and water continue animation WaterIodine The dialysis tubing membrane is selectively permeable The dialysis tubing membrane is selectively permeable Diffusion and Osmosis Slide 7 Quit Diffusion (Equipment Set Up) iodine and water starch, glucose and water starch, glucose and water DIALYSIS TUBING Diffusion and Osmosis Slide 8 Quit In DIFFUSION, particles travel from an area of high concentration to an area of low concentration As particles migrate through a membrane in this way, movement is described to be traveling down a concentration gradient Diffusion and Osmosis Slide 9 Quit When particles migrate through a selectively permeable membrane some particles are able to pass through but not others Diffusion and Osmosis Slide 10 Quit StarchGlucoseWater There is a GLUCOSE gradient across the membrane continue animation WaterIodine GLUCOSE molecules fit through the membrane and travel down a concentration gradient to the outside of the dialysis tubing testing indicates GLUCOSE now in dish NONE HIGH Diffusion and Osmosis Slide 11 Quit StarchGlucoseWater There is a IODINE gradient across the membrane continue animation Water Iodine IODINE molecules fit through the membrane and travel down a concentration gradient to the inside of the dialysis tubing testing indicates IODINE now in dialysis tubing as Starch turns PURPLE NONE HIGH Diffusion and Osmosis Slide 12 Quit StarchGlucoseWater There is a STARCH gradient across the membrane continue animation Water Iodine However, STARCH molecules cannot fit through the dialysis tubing membrane water in dish is not PURPLE NONE HIGH Diffusion and Osmosis Slide 13 Quit Starch Glucose Water There is a WATER gradient across the membrane continue animation WaterIodine WATER molecules fit through the membrane and travel down a concentration gradient to the inside of the dialysis tubing dialysis tubing is much fuller than before LOW HIGH Diffusion and Osmosis Slide 14 Quit Diffusion (Results) dialysis tubing in iodine and water Diffusion and Osmosis Slide 15 Quit OSMOSIS Osmometer Equipment Equipment Set Up Results Diffusion and Osmosis Slide 16 Quit OSMOSIS (Osmometer Equipment) pipet cover straw cup bell animal disc membrane animal disc membrane rubber band Diffusion and Osmosis Slide 17 Quit ASSEMBLING THE OSMOMETER cup with lid straw inserted into narrow end of bell bell opened at both ends selectively permeable membrane stretched across wide end of bell continue animation Diffusion and Osmosis Slide 18 Quit FILLING THE OSMOMETER The cup is filled one half full of water The bell is filled with a super saturated lactose solution (lactose and water) The solution is dyed to make it visible in the bell The position of the solution in the bell is marked continue animation Diffusion and Osmosis Slide 19 Quit A super saturated solution has a relatively low water concentration because of a VERY HIGH solute concentration The osmometer solution has a large amount of lactose solute dissolved in a small amount of water Diffusion and Osmosis Slide 20 Quit The osmometer is assembled continue animation Diffusion and Osmosis Slide 21 Quit Osmosis (Equipment Set Up) water super saturated lactose and water solution super saturated lactose and water solution fluid level Diffusion and Osmosis Slide 22 Quit OSMOMETER Diffusion of water takes place through the membrane. Water is pulled from an area of high concentration (in the cup) to an area of low concentration (in the bell) continue animation Diffusion and Osmosis Slide 23 Quit OSMOMETER As water diffuses through the membrane down the gradient from the cup into the bell the overall fluid level rises up the straw continue animation Diffusion and Osmosis Slide 24 Quit OSMOMETER The membrane is selectively permeable the lactose solute particles because of their larger size are prevented from migrating through the membrane and rise up the straw along with the water continue animation Diffusion and Osmosis Slide 25 Quit Osmosis (Results) fluid level water is pulled in through selectively permeable membrane water is pulled in through selectively permeable membrane osmometer solution rises up the straw osmometer solution rises up the straw Diffusion and Osmosis Slide 26 Quit LIVING CELL OSMOSIS Elodea Before NaCl Application 400x Elodea Before NaCl Application 1000x Elodea After NaCl Application 400x Elodea After NaCl Application 1000x Diffusion and Osmosis Slide 27 Quit Elodea Before NaCl Application 400x Diffusion and Osmosis Slide 28 Quit Elodea Before NaCl Application 1000x cell wall cell membrane chloroplasts continue animation Diffusion and Osmosis Slide 29 Quit The cell is initially in an isotonic solution Water within the cell keeps the cell membrane against the cell wall 100% water Chloroplasts are evenly spread throughout the cytoplasm continue animation Diffusion and Osmosis Slide 30 Quit Elodea 1000x continue animation The cell is placed in a hypertonic salt solution Diffusion and Osmosis Slide 31 Quit When the cell is placed in a hypertonic solution 100% water continue animation there is a salt gradient across the cell wall and membrane Water goes down the gradient from a high water concentration inside of the cell... to a low water concentration outside of the cell 90% water 10% salt Hypertonic Solution water Diffusion and Osmosis Slide 32 Quit Elodea After NaCl Application 400x continue animation Diffusion and Osmosis Slide 33 Quit The rigid cell wall is porous and remains intact... but without water inside of the cell, the cell membrane is pulled from the cell wall continue animation Hypertonic Solution water Hypertonic Solution Chloroplasts are draw in with the cell membrane and collect in the middle of the cell Diffusion and Osmosis Slide 34 Quit Elodea After NaCl Application 1000x chloroplasts cell wall cell membrane Water is pulled out of cell through the cell wall into hypertonic solution The cell membrane crenates pulling chloroplasts into a small bunch The cell membrane separates from the cell wall End Diffusion and Osmosis