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Quantitative traits of host-pathogen
interaction, and their contribution to
epidemic development in the wheat leaf
rust pathosystem.
H. Goyeau, G. Azzimonti, J. Papaïx, C. Lannou
Resistance components Aggressiveness components
Disease
HOST PATHOGEN
Quantitative traits of the host-pathogen
interaction
Assessment of quantitative traits
Growth Sporulation Infection
…In the glasshouse
A B
LP Latent Period SPL
SPS
LS
Sporulation per lesion
Per unit area
Lesion Size
40x
IE Infection Efficiency
Resistance components Aggressiveness components
HOST PATHOGEN
Disease
Quantitative traits of the host-pathogen
interaction
Questions :
• Relationships between different traits • Relationships between traits and field disease severity?
0
10
20
30
40
50
1999 2000 2001 2002 2003 2004 2005 2006 2007 2008
P3 P4 P5
Fréquence(%)
SID
CIE
CAMINS
BUS
FRA
LD7
AND
TRE
BAL
PBI
ECR
SOI
APA
0
0.2
0.4
0.6
0.8
1
RA
UD
PC
SID
CIE
CAMINS
BUS
FRA
LD7
AND
TRE
BAL
PBI
ECR
SOI
APA
0
0.2
0.4
0.6
0.8
1
RA
UD
PC
X
Experimental settings
13 Cultivars 3 PATHOTYPES
P3 P4 P5
2009 2010 2011
FIELD
3 years, 3 assessments per year
GLASSHOUSE
Quantitaive Traits (IE,
Latency, Sporulation)
Data Analysis
Distribution of variables
Bayesian Modelling -> Posterior Distribution
Regression to the estimated values
• Between quantitative traits • Between quantitative traits and field disease severity
• Quantitative traits (glasshouse) • Disease severity (field)
Correlations
PCA
Multiple colinearities
Simple linear Relationships between quantitative traits
LP
IE
SPS
IE
Positive rel. => increased R
LS
SPS
Negative rel. => Trade Off
SPL
SPS
NS (P=0,245)
Most of the components were correlated
Eigenvalues0
.00
.51
.01
.52
.02
.5 Multiple colinearities between quantitative traits
IE
SPS
SPL
LP
LS
PC1
PC2
Relationships between quantitative traits and field severity
IE
Date 1 Date 2 Date 3
All the quantitative traits were correlated with field disease severity, except for SPL.
P=1 P=1 P=1
Field
Disease
severity
Multiple Linear Regression
Disease severity at date i ~ PC1 * PC2
PC1 = axis 1 PCA (LP, LS, SPS and IE)
PC2 = axis 2 PCA (SPL)
Variable P value PC1 P value PC2
P value PC1*PC2
R2
Severity date 1
0.00029 ns ns 0.64
Severity date 2
0.00007 ns ns 0.72
Severity date 3
0.00005 ns ns 0.73
Infection Efficiency Latent Period Lesion Size Sporulation per unit area
Field disease severity correlated to
Multiple colinearities between quantitative traits and field severity
• Most of the quantitative traits were related (>0 or trade-off) [pleiotropic QTLs, trade-off]
• Field disease severity was correlated to quantitative traits assessed in controlled conditions [all the QTLs expressed in the glasshouse were expressed in the field]
• Assessment of quantitative traits in glasshouse experiments: – Different isolates => assessment of specificity
– Identification of diversified sources of resistance, to be combined
=> Durable Resistance
Conclusions
(Azzimonti et al., Plant Pathology, 2013)
Nicolas Lecutier
Anne-C. Zippert
Bénédicte Pariaud
Lucette Duveau
Thank you to …